{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:13:08Z","timestamp":1777525988742,"version":"3.51.4"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T00:00:00Z","timestamp":1538352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"},{"start":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T00:00:00Z","timestamp":1538352000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"name":"the Special Fund for Agro-scientific Research in the Public Interest","award":["201303046"],"award-info":[{"award-number":["201303046"]}]},{"name":"the Independent Innovation of Agricultural Sciences Program of Jiangsu Province","award":["cx (14)2089"],"award-info":[{"award-number":["cx (14)2089"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Vaccine immunization is now one of the most effective ways to control porcine reproductive and respiratory syndrome virus (PRRSV) infection. Impurity is one of the main factors affecting vaccine safety and efficacy. Here we present a novel innovative PRRSV purification approach based on surface display technology. First, a bifunctional protein PA-GRFT (protein anchor-griffithsin), the crucial factor in the purification process, was successfully produced in Escherichia coli yielding 80\u00a0mg\/L of broth culture. Then PRRSV purification was performed by incubation of PA-GRFT with PRRSV and gram-positive enhancer matrix (GEM) particles, followed by centrifugation to collect virions loaded onto GEM particles. Our results showed that most of the bulk impurities had been removed, and PA-GRFT could capture PRRSV onto GEM particles. Our lactic acid bacteria-based purification method, which is promising as ease of operation, low cost and easy to scale-up, may represent a candidate method for the large-scale purification of this virus for vaccine production.<\/jats:p>","DOI":"10.1007\/s10295-018-2061-1","type":"journal-article","created":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T15:26:56Z","timestamp":1532532416000},"page":"889-898","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Surface-displayed porcine reproductive and respiratory syndrome virus from cell culture onto gram-positive enhancer matrix particles"],"prefix":"10.1093","volume":"45","author":[{"given":"Lan","family":"Li","sequence":"first","affiliation":[{"name":"0000 0001 0017 5204 grid.454840.9 National Research, Center of Engineering and Technology for Veterinary Biologicals Jiangsu Academy of Agricultural Sciences 210014 Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuwen","family":"Qiao","sequence":"additional","affiliation":[{"name":"0000 0001 0017 5204 grid.454840.9 National Research, Center of Engineering and Technology for Veterinary Biologicals Jiangsu Academy of Agricultural Sciences 210014 Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Chen","sequence":"additional","affiliation":[{"name":"0000 0001 0017 5204 grid.454840.9 National Research, Center of Engineering and Technology for Veterinary Biologicals Jiangsu Academy of Agricultural Sciences 210014 Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanpeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"0000 0001 0017 5204 grid.454840.9 National Research, Center of Engineering and Technology for Veterinary Biologicals Jiangsu Academy of Agricultural Sciences 210014 Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qisheng","family":"Zheng","sequence":"additional","affiliation":[{"name":"0000 0001 0017 5204 grid.454840.9 National Research, Center of Engineering and Technology for Veterinary Biologicals Jiangsu Academy of Agricultural Sciences 210014 Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jibo","family":"Hou","sequence":"additional","affiliation":[{"name":"0000 0001 0017 5204 grid.454840.9 National Research, Center of Engineering and Technology for Veterinary Biologicals Jiangsu Academy of Agricultural Sciences 210014 Nanjing Jiangsu China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses 225009 Yangzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2018,10,1]]},"reference":[{"key":"2021033104031061700_CR1","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.jchromb.2016.01.060","article-title":"Purification of porcine reproductive and respiratory syndrome virus using ultrafiltration and liquid chromatography","volume":"1017\u20131018","author":"Bohua","year":"2016","journal-title":"J Chromatogr B Anal Technol Biomed Life Sci"},{"key":"2021033104031061700_CR2","doi-asserted-by":"publisher","first-page":"880","DOI":"10.1128\/AEM.72.1.880-889.2006","article-title":"Novel surface display system for proteins on non-genetically modified gram-positive bacteria","volume":"72","author":"Bosma","year":"2006","journal-title":"Appl Environ Microbiol"},{"key":"2021033104031061700_CR3","doi-asserted-by":"publisher","first-page":"838","DOI":"10.1111\/j.1365-2958.2008.06211.x","article-title":"LysM, a widely distributed 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