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In addition to their antibacterial activity, newly discovered 14-membered macrolides exhibit other therapeutic potentials, such as anti-proliferative and anti-protistal activities. Combinatorial biosynthetic approaches will allow us to create structurally diversified macrolide analogs, which are especially important during the emerging post-antibiotic era. This review focuses on recent advances in the discovery of new 14-membered macrolides (also including macrolactones) from microorganisms and the current status of combinatorial biosynthetic approaches, including polyketide synthase (PKS) and post-PKS tailoring pathways, and metabolic engineering for improved production together with heterologous production of 14-membered macrolides.<\/jats:p>","DOI":"10.1007\/s10295-018-2095-4","type":"journal-article","created":{"date-parts":[[2018,11,10]],"date-time":"2018-11-10T05:56:07Z","timestamp":1541829367000},"page":"445-458","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Recent advances in the discovery and combinatorial biosynthesis of microbial 14-membered macrolides and macrolactones"],"prefix":"10.1093","volume":"46","author":[{"given":"Je Won","family":"Park","sequence":"first","affiliation":[{"name":"0000 0001 0840 2678 grid.222754.4 School of Biosystem 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