{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:06:25Z","timestamp":1760043985513},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"},{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Rimocidin is a polyene macrolide that exhibits a strong inhibitory activity against a broad range of plant-pathogenic fungi. In this study, fermentation optimization and ribosome engineering technology were employed to enhance rimocidin production in Streptomyces rimosus M527. After the optimization of fermentation, rimocidin production in S. rimosus M527 increased from 0.11\u2009\u00b1\u20090.01 to 0.23\u2009\u00b1\u20090.02\u00a0g\/L during shake-flask experiments and reached 0.41\u2009\u00b1\u20090.05\u00a0g\/L using 5-L fermentor. Fermentation optimization was followed by the generation of mutants of S. rimosus M527 through treatment of the strain with different concentrations of gentamycin (Gen) or rifamycin. One Genr mutant named S. rimosus M527-G37 and one Rifr mutant named S. rimosus M527-R5 showed increased rimocidin production. Double-resistant (Genr and Rifr) mutants were selected using S. rimosus M527-G37 and S. rimosus M527-R5, and subsequently tested. One mutant, S. rimosus M527-GR7, which was derived from M527-G37, achieved the greatest cumulative improvement in rimocidin production. In the 5-L fermentor, the maximum rimocidin production achieved by S. rimosus M527-GR7 was 25.36% and 62.89% greater than those achieved by S. rimosus M527-G37 and the wild-type strain S. rimosus M527, respectively. Moreover, in the mutants S. rimosus M527-G37 and S. rimosus M527-GR7 the transcriptional levels of ten genes (rimA \u00a0sr to rimK \u00a0sr) located in the gene cluster involved in rimocidin biosynthesis were all higher than those in the parental strain M527 to varying degrees. In addition, after expression of the single rimocidin biosynthetic genes in S. rimosus M527 a few recombinants showed an increase in rimocidin production. Expression of rimE led to the highest production.<\/jats:p>","DOI":"10.1007\/s10295-019-02146-w","type":"journal-article","created":{"date-parts":[[2019,1,29]],"date-time":"2019-01-29T18:07:37Z","timestamp":1548785257000},"page":"697-708","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Sequential improvement of rimocidin production in <i>Streptomyces rimosus<\/i> M527 by introduction of cumulative drug-resistance mutations"],"prefix":"10.1093","volume":"46","author":[{"given":"Yanfang","family":"Zhao","sequence":"first","affiliation":[{"name":"0000 0004 1755 1108 grid.411485.d Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences China Jiliang University Xueyuan Street, Xiasha Higher Education District 310018 Hangzhou Zhejiang People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhangqing","family":"Song","sequence":"additional","affiliation":[{"name":"0000 0004 1755 1108 grid.411485.d Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences China Jiliang University Xueyuan Street, Xiasha Higher Education District 310018 Hangzhou Zhejiang People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Ma","sequence":"additional","affiliation":[{"name":"0000 0004 1755 1108 grid.411485.d Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences China Jiliang University Xueyuan Street, Xiasha Higher Education District 310018 Hangzhou Zhejiang People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Bechthold","sequence":"additional","affiliation":[{"name":"grid.5963.9 Institute for Pharmaceutical Sciences, Pharmaceutical Biology and Biotechnology University of Freiburg 79104 Freiburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoping","family":"Yu","sequence":"additional","affiliation":[{"name":"0000 0004 1755 1108 grid.411485.d Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences China Jiliang University Xueyuan Street, Xiasha Higher Education District 310018 Hangzhou Zhejiang People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,5,1]]},"reference":[{"issue":"18","key":"2021033103503605800_CR1","doi-asserted-by":"publisher","first-page":"6636","DOI":"10.1128\/AEM.05780-11","article-title":"New nystatin-related antifungal polyene macrolides with altered polyol region generated via biosynthetic engineering of Streptomyces 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