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These fat-selective images were subsequently subtracted from the MT-weighted 3D-UTE images to mitigate fat contamination in knee joint tissue imaging. This method was evaluated in an ex vivo human knee specimen and in three healthy volunteers at 3\u00a0T. Voxel-wise and ROI-based quantitative MT modeling were both performed to estimate macromolecular fraction (MMF), and results with and without fat suppression were compared.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Fat-selective images revealed off-resonance artifacts from surrounding fatty tissues that extended into adjacent joint structures. Subtraction of the fat-selective images effectively suppressed these artifacts and improved visualization of cartilage, meniscus, and tendons. In ex vivo data, fat suppression stabilized MMF estimates by correcting voxels with abnormally elevated values (&gt;\u200950%). MMF values were increased after fat suppression in articular cartilage (11.9\u2009\u00b1\u20091.3% vs. 17.3\u2009\u00b1\u20091.5%), meniscus (15.9\u2009\u00b1\u20090.8% vs. 18.2\u2009\u00b1\u20091.9%), patellar cartilage (13.5\u2009\u00b1\u20091.0% vs. 18.3\u2009\u00b1\u20090.4%), and patellar tendon (17.4\u2009\u00b1\u20092.8% vs. 24.6\u2009\u00b1\u20090.4%). Tissue contrast-to-noise ratios were also markedly improved.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Subtraction of fat-selective images provides a simple and effective approach for suppressing fat interference in UTE-MT imaging, improving both image contrast and MT measurements.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s10334-026-01370-7","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T15:35:32Z","timestamp":1779809732000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fat-suppressed quantitative ultrashort echo time magnetization transfer (UTE-MT) imaging of the knee joint"],"prefix":"10.1007","author":[{"given":"Soo Hyun","family":"Shin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyo S.","family":"Athertya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaji","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Lo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheronda M.","family":"Statum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine B.","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajun","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,26]]},"reference":[{"issue":"4","key":"1370_CR1","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1002\/jmri.24713","volume":"41","author":"EY Chang","year":"2015","unstructured":"Chang EY, Du J, Chung CB (2015) UTE imaging in the musculoskeletal system. 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