{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T16:59:17Z","timestamp":1785517157587,"version":"3.56.0"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100003790","name":"Hiroshima University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003790","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Jpn J Ophthalmol"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To investigate the prevalence and spectrum of microbial contamination of donor corneoscleral rims and storage medium in keratoplasty and to report a case of donor-derived fungal keratitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study design<\/jats:title>\n                    <jats:p>Single-center, retrospective study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We reviewed 285 eyes that underwent keratoplasty at Hiroshima University Hospital between October 2022 and January 2025. Donor corneas were preserved using Optisol\n                      <jats:sup>\u00ae<\/jats:sup>\n                      -GS. The donor rim and storage medium were separately cultured intraoperatively, and the minimum inhibitory concentrations (MICs) were determined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Culture positivity was identified in 10 of the 285 eyes (3.5%): six from donor rims and four from storage media, with no eye yielding positive results in both. All tested bacterial isolates were Gram-positive; three of six tested were not susceptible to levofloxacin and gatifloxacin (MIC \u22654\u00a0\u03bcg\/mL), including methicillin-resistant\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      , but retained susceptibility to vancomycin and meropenem. All fungal isolates were\n                      <jats:italic>Candida<\/jats:italic>\n                      with low MICs for voriconazole and micafungin.\n                      <jats:italic>C. albicans<\/jats:italic>\n                      and\n                      <jats:italic>C. glabrata<\/jats:italic>\n                      were recovered from the mate corneas of a single donor; the recipient of the cornea contaminated with\n                      <jats:italic>C. albicans<\/jats:italic>\n                      developed keratitis requiring repeat penetrating keratoplasty, whereas the recipient of the\n                      <jats:italic>C. glabrata-<\/jats:italic>\n                      contaminated cornea did not.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Adding storage medium to the rim culture improved the detection of donor-associated microorganisms. When donor-derived\n                      <jats:italic>C. albicans<\/jats:italic>\n                      keratitis developed, susceptibility data from contemporaneous cultures enabled prompt targeted antifungal therapy and globe preservation, although repeated penetrating keratoplasty was ultimately required.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s10384-026-01402-8","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T09:03:45Z","timestamp":1785143025000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microbial contamination of donor corneoscleral rims and storage medium in keratoplasty"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0539-884X","authenticated-orcid":false,"given":"Atsuhiko","family":"Fukuto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzu","family":"Deie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurie","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Sakaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiichiro","family":"Chikama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,27]]},"reference":[{"key":"1402_CR1","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1001\/archopht.123.5.605","volume":"123","author":"M Taban","year":"2005","unstructured":"Taban M, Behrens A, Newcomb RL, Nobe MY, McDonnell PJ. 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Fukuto, Grants or contracts to the author\u2019s institution (AMO, Alcon), Payment or honoraria for lectures, presenta tions, speakers bureaus, manuscript writing or educational events (Nidek, Senju, Santen); S. Deie, Payment or hono raria for lectures, presentations, speakers bureaus, manu script writing or educational events (Senju);Y. Shimizu, Payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing or educational events (Senju); H. Sakaguchi, Lecture fee (Santen, Senju, Chugai, Alcon, HOYA, Ono, AMO); T. Chikama, Grants (Tarsus), Consult ing fees (Aurion Biotech, J-TEC, Tarsus), Lecture honorar ium (Rohto-Nitten, Viatris, Santen, Senju, Chugai, GSK, Amgen, Daiichi Sankyo, Aurion Biotech, CynosBio, Kowa, Genmab, J-TEC), Travel support for conference attend ance (Tarsus), Advisory board \/ advisory contract (Aurion Biotech)","order":1,"name":"Ethics","label":"Conflicts of interest","group":{"name":"EthicsHeading","label":"Declarations"}}]}}