{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T22:03:21Z","timestamp":1776204201237,"version":"3.50.1"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2014,9,18]],"date-time":"2014-09-18T00:00:00Z","timestamp":1410998400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EcoHealth"],"published-print":{"date-parts":[[2015,3]]},"DOI":"10.1007\/s10393-014-0973-4","type":"journal-article","created":{"date-parts":[[2014,9,17]],"date-time":"2014-09-17T18:34:47Z","timestamp":1410978887000},"page":"183-187","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested-PCR"],"prefix":"10.1007","volume":"12","author":[{"given":"Ana S.","family":"Faria","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria das Neves","family":"Paiva-Cardoso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica","family":"Nunes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Carreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9lia M.","family":"Vale-Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oct\u00e1via","family":"Veloso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catarina","family":"Coelho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o A.","family":"Cabral","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madalena","family":"Vieira-Pinto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria L.","family":"Vieira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2014,9,18]]},"reference":[{"key":"973_CR1","first-page":"109","volume":"293","author":"S Baptista","year":"2004","unstructured":"Baptista S, Quaresma A, Aires T, Kurtenbach K, Santos-Reis M, Nicholson M, Collares-Pereira M (2004) Lyme borreliosis spirochetes in questing ticks from mainland Portugal. International Journal of Medical Microbiology 293:109-116. doi: 10.1016\/S1433-1128(04)80016-0","journal-title":"International Journal of Medical Microbiology"},{"key":"973_CR2","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1186\/1471-2105-10-421","volume":"10","author":"C Camacho","year":"2008","unstructured":"Camacho C, Coulouris G, Avagyan V, Ma\u00a0N, Papadopoulos J, Bealer K, Madden TL (2008) BLAST\u00a0+\u00a0: architecture and applications. BMC Bioinformatics 10:421. doi: 10.1186\/1471-2105-10-421","journal-title":"BMC Bioinformatics"},{"issue":"7","key":"973_CR3","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1023\/A:1007420008690","volume":"13","author":"F Christiann","year":"1997","unstructured":"Christiann F, Rayet P, Patey O, Ngueodjibaye DB, Theron-le Gargasson JF, Lafaix C (1997) Lyme borreliosis in central France: a sero-epidemiologic examination involving hunters. European Journal of Epidemiology 13(7):855. doi: 10.1023\/A:1007420008690","journal-title":"European Journal of Epidemiology"},{"issue":"3","key":"973_CR4","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1128\/JCM.42.3.1316-1318.2004","volume":"42","author":"M Collares-Pereira","year":"2004","unstructured":"Collares-Pereira M, Couceiro S, Franca I, Kurtenbach K, Sch\u00e4fer SM, Vitorino L, Gon\u00e7alves L, Baptista S, Vieira ML, Cunha C (2004) First isolation of Borrelia lusitaniae from a human patient. Journal of Clinical Microbiology 42(3):1316-1318. doi: 10.1128\/JCM.42.3.1316-1318.2004","journal-title":"Journal of Clinical Microbiology"},{"issue":"11","key":"973_CR5","doi-asserted-by":"crossref","first-page":"e81337","DOI":"10.1371\/journal.pone.0081337","volume":"8","author":"P Costa","year":"2013","unstructured":"Costa P, Ferreira AS, Amaro A, Albuquerque T, Botelho A, Couto I, Cunha MV, Viveiros M, In\u00e1cio J (2013) Enhanced detection of tuberculous mycobacteria in animal tissues using a semi-nested probe-based real-time PCR. PLoS ONE 8(11):e81337. doi: 10.1371\/journal.pone.0081337","journal-title":"PLoS ONE"},{"issue":"2","key":"973_CR6","first-page":"77","volume":"28","author":"S Couceiro","year":"2003","unstructured":"Couceiro S, Baptista S, Franca I, Gon\u00e7alves L, Vieira ML, Collares-Pereira M (2003) Cultura vs PCR: que apoio ao diagn\u00f3stico de Borreliose de Lyme? Acta Reumatol\u00f3gica Portuguesa 28(2):77-82","journal-title":"Acta Reumatol\u00f3gica Portuguesa"},{"key":"973_CR7","first-page":"261","volume":"12","author":"JA David de Morais","year":"1989","unstructured":"David de Morais JA, Filipe AR, N\u00fancio MS (1989) Doen\u00e7a de Lyme em Portugal: caso cl\u00ednico. Revista Portuguesa de Doen\u00e7as Infecciosas 12:261-274","journal-title":"Revista Portuguesa de Doen\u00e7as Infecciosas"},{"issue":"6","key":"973_CR8","doi-asserted-by":"crossref","first-page":"2128","DOI":"10.1128\/JCM.38.6.2128-2133.2000","volume":"38","author":"S Michelis De","year":"2000","unstructured":"De Michelis S, Sewell HS, Collares-Pereira M, Santos-Reis M, Schouls LM, Benes V, Holmes EC, Kurtenbach K (2000) Genetic diversity of Borrelia burgdorferi sensu lato in ticks from mainland Portugal. Journal of Clinical Microbiology 38(6):2128-2133","journal-title":"Journal of Clinical Microbiology"},{"issue":"1","key":"973_CR9","first-page":"55","volume":"167","author":"JM Doby","year":"1991","unstructured":"Doby JM, Betremieux C, Rolland C, Barrat J (1991) The large forest mammals reservoirs for Borrelia burgdorferi agent of the lyme disease? Serological examination of 543 deers and wild boars. Recueil de M\u00e9decine V\u00e9t\u00e9rinaire 167(1):55-61","journal-title":"Recueil de M\u00e9decine V\u00e9t\u00e9rinaire"},{"key":"973_CR10","unstructured":"ECDC (2014) Emerging and Vector-borne Diseases Programme. http:\/\/www.ecdc.europa.eu\/en\/activities\/diseaseprogrammes\/emerging_and_vector_borne_diseases\/Pages\/index.aspx . Accessed May 28, 2014"},{"issue":"3\u20134","key":"973_CR11","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/s10493-005-3271-6","volume":"37","author":"A Estrada-Pe\u00f1a","year":"2005","unstructured":"Estrada-Pe\u00f1a A, Os\u00e1car JJ, Pichon B, Gray JS (2005) Hosts and pathogen detection for immature stages of Ixodes ricinus (Acari: Ixodidae) in North-Central Spain. Experimental & Applied Acarology 37(3-4):257-268. doi: 10.1007\/s10493-005-3271-6","journal-title":"Experimental & Applied Acarology"},{"key":"973_CR12","unstructured":"EUCALB (2014) European Concerted Action on Lyme Borreliosis (EUCALB). http:\/\/meduni09.edis.at\/eucalb\/cms_15\/index.php . Accessed May 28, 2014"},{"key":"973_CR13","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1159\/000213068","volume":"37","author":"L Gern","year":"2009","unstructured":"Gern L (2009) Life cycle of Borrelia burgdorferi sensu lato and transmission to humans. Current Problems in Dermatology. 37:18-30. doi: 10.1159\/000213068","journal-title":"Current Problems in Dermatology"},{"key":"973_CR14","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1159\/000213069","volume":"37","author":"Z Hubalek","year":"2009","unstructured":"Hubalek Z (2009) Epidemiology of Lyme Borreliosis. Current Problems in Dermatology 37:31-50. doi: 10.1159\/000213069","journal-title":"Current Problems in Dermatology"},{"issue":"3","key":"973_CR15","first-page":"331","volume":"51","author":"Z Juricova","year":"1996","unstructured":"Juricova Z, Halouzka J, Forejtek P, Hubalek Z (1996) Detection of antibodies to Borrelia burgdorferi in hares, wild boars and pheasants in the Czech Republic. Biologia 51(3):331-333","journal-title":"Biologia"},{"key":"973_CR16","first-page":"145","volume":"45","author":"Z Juricova","year":"2000","unstructured":"Juricova Z, Halouzka J, Hubalek Z (2000) Prevalence of antibodies to Borrelia burgdorferi in game animals in south Moravia, Czech Republic. Veterin\u00e1rn\u00ed Medic\u00edna 45:145-148","journal-title":"Veterin\u00e1rn\u00ed Medic\u00edna"},{"issue":"5","key":"973_CR17","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1089\/vbz.2008.0125","volume":"9","author":"Z Juricova","year":"2009","unstructured":"Juricova Z, Hubalek Z (2009) Serologic survey of the wild boar (Sus scrofa) for Borrelia burgdorferi sensu lato. Vector Borne and Zoonotic Diseases 9(5):479-482. doi: 10.1089\/vbz.2008.0125","journal-title":"Vector Borne and Zoonotic Diseases"},{"issue":"2","key":"973_CR18","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1111\/j.1948-7134.2013.12050.x","volume":"38","author":"D Kiewra","year":"2013","unstructured":"Kiewra D, Zale\u015bny G (2013) Relationship between temporal abundance of ticks and incidence of Lyme borreliosis in Lower Silesia regions of Poland. Journal of Vector Ecology 38(2):345\u2013352. doi: 10.1111\/j.1948-7134.2013.12050.x","journal-title":"Journal of Vector Ecology"},{"issue":"10","key":"973_CR19","doi-asserted-by":"crossref","first-page":"4926","DOI":"10.1128\/AEM.67.10.4926-4929.2001","volume":"67","author":"K Kurtenbach","year":"2001","unstructured":"Kurtenbach K, De Michelis S, Sewell HS, Etti S, Sch\u00e4fer SM, Hails R, Collares-Pereira M, Santos-Reis M, Hanin\u00e7ov\u00e1 K, Labuda M, Bormane A, Donaghy M (2001) Distinct combinations of Borrelia burgdorferi sensu lato genospecies found in individual questing ticks from Europe. Applied and Environmental Microbiology 67(10):4926-4929. doi: 10.1128\/AEM.67.10.4926-4929.2001","journal-title":"Applied and Environmental Microbiology"},{"issue":"4","key":"973_CR20","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1099\/00207713-47-4-921","volume":"47","author":"A Fleche Le","year":"1997","unstructured":"Le Fleche A, Postic D, Girardet K, Peter O, Baranton G (1997) Characterization of Borrelia lusitaniae sp. nov. by 16S ribosomal DNA sequence analysis. International Journal of Systematic Bacteriology 47(4):921-925. doi: 10.1099\/00207713-47-4-921","journal-title":"International Journal of Systematic Bacteriology"},{"key":"973_CR21","unstructured":"Lindgren E, Jaenson TGT (2006) Lyme Borreliosis in Europe: influences of climate and climate change, epidemiology, ecology and adaptation measures. WHO Regional Office for Europe. http:\/\/www.euro.who.int\/en\/publications\/abstracts\/lyme-borreliosis-in-europe.-influences-of-climate-and-climate-change,-epidemiology,-ecology-and-adaptation-measures . Accessed May 28, 2014"},{"issue":"10","key":"973_CR22","doi-asserted-by":"crossref","first-page":"257","DOI":"10.2807\/esm.11.10.00650-en","volume":"11","author":"I Lopes de Carvalho","year":"2006","unstructured":"Lopes de Carvalho I, N\u00fancio MS (2006) Laboratory diagnosis of Lyme borreliosis at the Portuguese National Institute of Health (1990-2004). Eurosurveillance 11(10):257-60","journal-title":"Eurosurveillance"},{"issue":"5","key":"973_CR23","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1089\/vbz.2008.0210","volume":"10","author":"I Lopes de Carvalho","year":"2010","unstructured":"Lopes de Carvalho I, Zeidner N, Ullmann A, Hojgaard A, Amaro F, Z\u00e9-Z\u00e9 L, Alves MJ, de Sousa R, Piesman J, Nuncio MS (2010) Molecular characterization of a new isolate of Borrelia lusitaniae derived from Apodemus sylvaticus in Portugal. Vector Borne and Zoonotic Diseases 10(5):531-534. doi: 10.1089\/vbz.2008.0210","journal-title":"Vector Borne and Zoonotic Diseases"},{"issue":"2","key":"973_CR24","doi-asserted-by":"crossref","first-page":"186","DOI":"10.2460\/javma.1994.205.02.186","volume":"205","author":"TN Mather","year":"1994","unstructured":"Mather TN, Fish D, Coughlin RT (1994) Competence of dogs as reservoirs for Lyme disease spirochetes (Borrelia burgdorferi). Journal of the American Veterinary Medical Association 205(2):186-8","journal-title":"Journal of the American Veterinary Medical Association"},{"issue":"6","key":"973_CR25","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1093\/jmedent\/44.6.1109","volume":"44","author":"F M\u00f3ran Cadenas","year":"2007","unstructured":"M\u00f3ran Cadenas F, Rais O, Humair PF, Douet V, Moret J, Gern L (2007) Identification of host bloodmeal source and Borrelia burgdorferi sensu lato in field-collected Ixodes ricinus ticks in Chaumont (Switzerland). Journal of Medical Entomology 44(6):1109-1117. doi: 10.1603\/0022-2585(2007)44[1109:IOHBSA]2.0.CO;2","journal-title":"Journal of Medical Entomology"},{"issue":"2","key":"973_CR26","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1111\/j.1462-2920.2012.02834.x","volume":"15","author":"AC Norte","year":"2012","unstructured":"Norte AC, Ramos JA, Gern L, N\u00fancio MS, Lopes de Carvalho I (2012) Birds as reservoirs for Borrelia burgdorferi s.l. in Western Europe: circulation of B. turdi and other genospecies in bird\u2013tick cycles in Portugal. Environmental Microbiology 15(2):386-397. doi: 10.1111\/j.1462-2920.2012.02834.x","journal-title":"Environmental Microbiology"},{"key":"973_CR27","first-page":"175","volume":"16","author":"M N\u00fancio","year":"1993","unstructured":"N\u00fancio M, P\u00e9ter O, Alves M, Bacellar F, Filipe A (1993) Isolamento e caracteriza\u00e7\u00e3o de borr\u00e9lias de Ixodes ricinus em Portugal. Revista Portuguesa de Doen\u00e7as Infecciosas 16:175-179","journal-title":"Revista Portuguesa de Doen\u00e7as Infecciosas"},{"issue":"12","key":"973_CR28","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.3201\/eid0812.010519","volume":"8","author":"D Richter","year":"2002","unstructured":"Richter D, Allgower R, Matuschka FR (2002) Co-feeding transmission and its contribution to the perpetuation of the Lyme disease spirochete Borrelia afzelii. Emerging Infectious Diseases 8(12):1421-1425. doi: 10.3201\/eid0812.010519","journal-title":"Emerging Infectious Diseases"},{"issue":"27","key":"973_CR29","first-page":"19906","volume":"16","author":"A Rizzoli","year":"2011","unstructured":"Rizzoli A, Hauffe H, Carpi G, Vourc HG, Neteler M, Rosa R (2011) Lyme borreliosis in Europe. Eurosurveillance 16(27):19906","journal-title":"Lyme borreliosis in Europe. Eurosurveillance"},{"key":"973_CR30","volume-title":"Reconocimiento de sexo y edad en especies cineg\u00e9ticas","author":"M S\u00e1enz de Buruaga","year":"1991","unstructured":"S\u00e1enz de Buruaga M, Lucio AJ, Purroy FJ (1991) Reconocimiento de sexo y edad en especies cineg\u00e9ticas. Vitoria-Gasteiz, Spain: Diputaci\u00f3n Foral de Alava"},{"issue":"5","key":"973_CR31","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1089\/vbz.2013.1495","volume":"14","author":"C Silaghi","year":"2014","unstructured":"Silaghi C, Pfister K, Overzier E (2014) Molecular investigation for bacterial and protozoan tick-borne pathogens in wild boars (Sus scrofa) from southern Germany. Vector-Borne and Zoonotic Diseases 14(5):371-373. doi: 10.1089\/vbz.2013.1495","journal-title":"Vector-Borne and Zoonotic Diseases"},{"issue":"1","key":"973_CR32","first-page":"47","volume":"41","author":"M Situm","year":"2000","unstructured":"Situm M, Grahovac B, Markovi\u0107 S, Lipozenci\u0107 J, Poje G, Dobri\u0107 I, Marinovi\u0107 B, Bolanca-Bumber S, Misi\u0107-Majerus L (2000) Detection and genotyping of Borrelia burgdorferi sensu lato by polymerase chain reaction. Croatian Medical Journal 41(1):47-53","journal-title":"Croatian Medical Journal"},{"issue":"12","key":"973_CR33","doi-asserted-by":"crossref","first-page":"668","DOI":"10.17221\/1975-VETMED","volume":"53","author":"B Skotarczak","year":"2008","unstructured":"Skotarczak B, Adamska M, Sawczuk M, Maciejewska A, Wodecka B, Rymaszewska A (2008) Coexistence of tick-borne pathogens in game animals and ticks in western Poland. Veterin\u00e1rn\u00ed Medic\u00edna 53(12):668\u2013675","journal-title":"Veterin\u00e1rn\u00ed Medic\u00edna"},{"key":"973_CR100","unstructured":"Wodecka B (2007) Significance of red deer (Cervus elaphus) in the ecology of Borrelia burgdorferi sensu lato. Wiadomo\u015bci Parazytologiczne 53(3):231\u2013237"},{"issue":"1","key":"973_CR34","first-page":"9","volume":"16","author":"B Wodecka","year":"2009","unstructured":"Wodecka B, Rymaszewska A, Sawczuk M, Skotarczak B (2009) Detectability of tick-borne agents DNA in the blood of dogs undergoing treatment for Borreliosis. Annals of Agricultural and Environmental Medicine 16(1):9\u201314","journal-title":"Annals of Agricultural and Environmental Medicine"},{"issue":"3","key":"973_CR35","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1099\/jmm.0.013508-0","volume":"59","author":"B Wodecka","year":"2010","unstructured":"Wodecka B, Leo\u0144ska A, Skotarczak B (2010) A comparative analysis of molecular markers for the detection and identification of Borrelia spirochaetes in Ixodes ricinus. Journal of Medical Microbiology 59(3):309-314. doi: 10.1099\/jmm.0.013508-0","journal-title":"Journal of Medical Microbiology"},{"issue":"4","key":"973_CR36","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1007\/s10493-013-9763-x","volume":"62","author":"B Wodecka","year":"2014","unstructured":"Wodecka B, Rymaszewska A, Skotarczak B (2014) Host and pathogen DNA identification in blood meals of nymphal Ixodes ricinus ticks from forest parks and rural forests of Poland. Experimental & Applied Acarology 62(4):543-55. doi: 10.1007\/s10493-013-9763-x","journal-title":"Experimental & Applied Acarology"},{"issue":"4","key":"973_CR37","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S0147-9571(99)00015-6","volume":"22","author":"P Zeman","year":"1999","unstructured":"Zeman P, Januska J (1999) Epizootiologic background of dissimilar distribution of human cases of Lyme borreliosis and tick-borne encephalitis in a joint endemic area. Comparative Immunology, Microbiology and Infectious Diseases 22(4):247-60. doi: 10.1016\/S0147-9571(99)00015-6","journal-title":"Comparative Immunology, Microbiology and Infectious Diseases"}],"container-title":["EcoHealth"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10393-014-0973-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10393-014-0973-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10393-014-0973-4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,2]],"date-time":"2024-06-02T16:46:24Z","timestamp":1717346784000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10393-014-0973-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,18]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["973"],"URL":"https:\/\/doi.org\/10.1007\/s10393-014-0973-4","relation":{},"ISSN":["1612-9202","1612-9210"],"issn-type":[{"value":"1612-9202","type":"print"},{"value":"1612-9210","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,18]]}}}