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Imaging studies revealing morphological changes in these soft tissues have not distinguished between superficial and intramuscular adipose distribution, despite the recognition that intramuscular fat levels indicate reduced tolerance to mechanical loading. Furthermore, it is unclear how these changes may alter tissue tone and stiffness, which are key features in prosthetic socket design. This study was designed to compare the morphology and biomechanical response of limb tissues to mechanical loading in individuals with and without transtibial amputation, using magnetic resonance imaging in combination with tissue structural stiffness. The results revealed higher adipose infiltrating muscle in residual limbs than in intact limbs (residual: median 2.5% (range 0.2\u20138.9%); contralateral: 1.7% (0.1\u20135.1%); control: 0.9% (0.4\u20131.3%)), indicating muscle atrophy and adaptation post-amputation. The intramuscular adipose content correlated negatively with daily socket use, although there was no association with time post-amputation. Residual limbs were significantly stiffer than intact limbs at the patellar tendon site, which plays a key role in load transfer across the limb-prosthesis interface. The tissue changes following amputation have relevance in the clinical understanding of prosthetic socket design variables and soft tissue damage risk in this vulnerable group.<\/jats:p>","DOI":"10.1007\/s10439-021-02858-0","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T14:21:47Z","timestamp":1632752507000},"page":"3176-3188","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Changes in Tissue Composition and Load Response After Transtibial Amputation Indicate Biomechanical Adaptation"],"prefix":"10.1007","volume":"49","author":[{"given":"J. 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