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This study investigated whether specific micro-topographies can modulate platelet deposition under LVAD supraphysiological wall shear stress (WSS) conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      A custom microfluidic platform was developed to generate a linear WSS gradient from 16 to 130\u00a0Pa. Microchannels were patterned with reverse cones and hemispheres in small (1\u20133\u00a0\n                      <jats:italic>\u00b5<\/jats:italic>\n                      m) and large (3\u20139\u00a0\n                      <jats:italic>\u00b5<\/jats:italic>\n                      m) sizes using two-photon polymerization and nanoimprinting. Human blood was perfused through the microchannels, and platelet deposition was quantified over time as the area coverage ratio (\n                      <jats:italic>A<\/jats:italic>\n                      \u209a\u2097\u209c\/\n                      <jats:italic>A<\/jats:italic>\n                      \u209c\u2092\u209c) and area under the curve (AUC). Platelet detachment events were counted as an indicator of thrombus stability, and computational simulations supported the interpretation of local shear conditions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Consistent trends emerged,\u00a0although no statistical differences were observed relative to flat controls.\n                      <jats:italic>A<\/jats:italic>\n                      \u209a\u2097\u209c\/\n                      <jats:italic>A<\/jats:italic>\n                      \u209c\u2092\u209c increased with WSS for all surfaces. At 16\u00a0Pa, small and large cones reduced platelet adhesion by approximately 84 and 98%, respectively, compared to flat controls. At 49\u00a0Pa, the reduction was about 95% for small cones and 80% for large cones. Conical geometries also promoted platelet washout at higher WSS. Small hemispherical features showed more than 50% lower platelet adhesion than flat surfaces for WSS\u2009&gt;\u200916\u00a0Pa, with limited thrombus growth.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Conical micropatterns may be most effective at limiting platelet adhesion at lower WSS, while small hemispheres may perform better at higher WSS. These trends suggest a surface\u2013shear interaction that warrants further investigation for LVAD surface optimization.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s10439-026-04108-7","type":"journal-article","created":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T20:17:48Z","timestamp":1774642668000},"page":"4082-4093","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Influence of Micropatterned Surfaces on Platelet Adhesion to Reduce the Risk of Thrombus Formation in Left Ventricular Assist Devices"],"prefix":"10.1007","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3667-0965","authenticated-orcid":false,"given":"Marta","family":"Bonora","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stjepan","family":"Perak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Kopp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josu","family":"Garcia Morales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Benauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Lunzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"M\u00fchlberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Ruthmeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Messner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael Bernhard","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Moscato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,3,27]]},"reference":[{"key":"4108_CR1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-10-636480","author":"A Nascimbene","year":"2016","unstructured":"Nascimbene, A., S. 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