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ECM synthesis and degradation was quantified in cartilage explants, human mesenchymal stem cells, and SKBR3 breast cancer cells under various external stimuli, including inflammation, mechanical stimulus, and drug treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The click chemistry method reliable quantified ECM turnover across platforms. It detected reduced glycan and protein synthesis after 24-hour inflammatory challenge and enabled longitudinal tracking of ECM degradation in cartilage explants. The technique demonstrated high sensitivity, measuring increased ECM deposition by\u2009~\u200910,000 human mesenchymal stem cells in 12-hour intervals and substrate stiffness-dependent synthesis by SKBR3 cells. Additionally, the approach supported osteoarthritis drug screening by identifying compounds that mitigated inflammation-induced ECM degradation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Compared to traditional biochemical or histological assays, the click chemistry-based technique provides higher sensitivity, reduced sample requirements, and improved temporal resolution for quantifying ECM turnover. Its versatility enables broad application in tissue engineering, regenerative medicine, disease modeling, and high-throughput drug evaluation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s10439-026-04152-3","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T19:15:03Z","timestamp":1778613303000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Click Chemistry-Based Quantification of Extracellular Matrix Turnover for Drug Screening and Regenerative Medicine"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8207-0357","authenticated-orcid":false,"given":"Annie","family":"Porter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songshan","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengxi","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilu","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz","family":"Alanazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6905-6887","authenticated-orcid":false,"given":"X. 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