{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:57:08Z","timestamp":1790207828211,"version":"4.1.0"},"reference-count":46,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T00:00:00Z","timestamp":1679875200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T00:00:00Z","timestamp":1679875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Angiogenesis"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>APJ has been extensively described in the pathophysiology of angiogenesis and cell proliferation. The prognostic value of APJ overexpression in many diseases is now established. This study aimed to design a PET radiotracer that specifically binds to APJ. Apelin-F13A-NODAGA (AP747) was synthesized and radiolabeled with gallium-68 ([<jats:sup>68<\/jats:sup>Ga]Ga-AP747). Radiolabeling purity was excellent (&gt;\u200995%) and stable up to 2\u00a0h. Affinity constant of [<jats:sup>67<\/jats:sup>Ga]Ga-AP747 was measured on APJ-overexpressing colon adenocarcinoma cells and was in nanomolar range. Specificity of [<jats:sup>68<\/jats:sup>Ga]Ga-AP747 for APJ was evaluated in vitro by autoradiography and in vivo by small animal PET\/CT in both colon adenocarcinoma mouse model and Matrigel plug mouse model. Dynamic of [<jats:sup>68<\/jats:sup>Ga]Ga-AP747 PET\/CT biodistributions was realized on healthy mice and pigs for two hours, and quantification of signal in organs showed a suitable pharmacokinetic profile for PET imaging, largely excreted by urinary route. Matrigel mice and hindlimb ischemic mice were submitted to a 21-day longitudinal follow-up with [<jats:sup>68<\/jats:sup>Ga]Ga-AP747 and [<jats:sup>68<\/jats:sup>Ga]Ga-RGD<jats:sub>2<\/jats:sub> small animal PET\/CT. [<jats:sup>68<\/jats:sup>Ga]Ga-AP747 PET signal in Matrigel was significantly more intense than that of [<jats:sup>68<\/jats:sup>Ga]Ga-RGD<jats:sub>2<\/jats:sub>. Revascularization of the ischemic hind limb was followed by LASER Doppler. In the hindlimb, [<jats:sup>68<\/jats:sup>Ga]Ga-AP747 PET signal was more than twice higher than that of [<jats:sup>68<\/jats:sup>Ga]Ga-RGD<jats:sub>2<\/jats:sub> on day 7, and significantly superior over the 21-day follow-up. A significant, positive correlation was found between the [<jats:sup>68<\/jats:sup>Ga]Ga-AP747 PET signal on day 7 and late hindlimb perfusion on day 21. We developed a new PET radiotracer that specifically binds to APJ, [<jats:sup>68<\/jats:sup>Ga]Ga-AP747 that showed more efficient imaging properties than the most clinically advanced tracer of angiogenesis, [<jats:sup>68<\/jats:sup>Ga]Ga-RGD<jats:sub>2<\/jats:sub>.\n<\/jats:p>","DOI":"10.1007\/s10456-023-09875-8","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T12:04:42Z","timestamp":1679918682000},"page":"463-475","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Design and preclinical evaluation of a novel apelin-based PET radiotracer targeting APJ receptor for molecular imaging of angiogenesis"],"prefix":"10.1007","volume":"26","author":[{"given":"B\u00e9atrice","family":"Louis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Nail","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oriane","family":"Nachar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahlem","family":"Bouhlel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana\u00efs","family":"Moyon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Balasse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Simoncini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrien","family":"Chabert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Fernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Brige","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Hache","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aura","family":"Tintaru","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9ment","family":"Morgat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Dignat-George","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Garrigue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Guillet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,3,27]]},"reference":[{"key":"9875_CR1","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1007\/s10967-019-06654-y","volume":"321","author":"H Ma","year":"2019","unstructured":"Ma H, Liu S, Zhang Z et al (2019) Preliminary biological evaluation of 68Ga-labeled cyclic RGD dimer as an integrin \u03b1v\u03b23-targeting radiotracer for tumor PET imaging. J Radioanal Nucl Chem 321:857\u2013865","journal-title":"J Radioanal Nucl Chem"},{"key":"9875_CR2","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1177\/1535370220911604","volume":"245","author":"Y Xiao","year":"2020","unstructured":"Xiao Y, Wang T, Song X, Yang D, Chu Q, Kang YJ (2020) Copper promotion of myocardial regeneration. Exp Biol Med 245:911\u2013921","journal-title":"Exp Biol Med"},{"key":"9875_CR3","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1007\/s10456-017-9562-9","volume":"20","author":"C Viallard","year":"2017","unstructured":"Viallard C, Larriv\u00e9e B (2017) Tumor angiogenesis and vascular normalization: alternative therapeutic targets. Angiogenesis 20:409\u2013426","journal-title":"Angiogenesis"},{"key":"9875_CR4","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2021.104189","volume":"138","author":"G Armani","year":"2021","unstructured":"Armani G, Pozzi E, Pagani A et al (2021) The heterogeneity of cancer endothelium: the relevance of angiogenesis and endothelial progenitor cells in cancer microenvironment. Microvasc Res 138:104189","journal-title":"Microvasc Res"},{"key":"9875_CR5","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1007\/s11307-018-1255-2","volume":"20","author":"F Man","year":"2018","unstructured":"Man F, Lammers T, de Rosales TMR (2018) Imaging Nanomedicine-based drug delivery: a review of clinical studies. Mol Imaging Biol 20:683\u2013695","journal-title":"Mol Imaging Biol"},{"key":"9875_CR6","first-page":"15","volume":"8","author":"I Velikyan","year":"2018","unstructured":"Velikyan I, Lindhe \u00d6 (2018) Preparation and evaluation of a 68Ga-labeled RGD-containing octapeptide for noninvasive imaging of angiogenesis: biodistribution in non-human primate. Am J Nucl Med Mol Imaging 8:15\u201331","journal-title":"Am J Nucl Med Mol Imaging"},{"key":"9875_CR7","doi-asserted-by":"publisher","DOI":"10.4274\/mirt.30502","author":"MF Bozkurt","year":"2018","unstructured":"Bozkurt MF, \u00d6zcan Z (2018) The evolving role of nuclear medicine and molecular imaging: theranostics and personalized therapeutic applications. Mol Imaging Radionucl Ther. https:\/\/doi.org\/10.4274\/mirt.30502","journal-title":"Mol Imaging Radionucl Ther"},{"key":"9875_CR8","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1016\/j.acvd.2019.03.005","volume":"112","author":"E Donal","year":"2019","unstructured":"Donal E, Lafitte S, Sportouch C (2019) The new place of imaging in cardiology, from diagnosis to treatment. Arch Cardiovasc Dis 112:543\u2013545","journal-title":"Arch Cardiovasc Dis"},{"key":"9875_CR9","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1053\/j.semnuclmed.2019.02.002","volume":"49","author":"A Farolfi","year":"2019","unstructured":"Farolfi A, Lima GM, Oyen W, Fanti S (2019) Molecular imaging and theranostics\u2014A multidisciplinary approach. Semin Nucl Med 49:247\u2013254","journal-title":"Semin Nucl Med"},{"key":"9875_CR10","doi-asserted-by":"crossref","unstructured":"Wu M, Shu J. (2018) Multimodal molecular imaging: current status and future directions. Contrast Media Mol Imaging. vol 2018. https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6008764\/. Accessed 16 Apr 2020","DOI":"10.1155\/2018\/1382183"},{"key":"9875_CR11","doi-asserted-by":"publisher","first-page":"4821","DOI":"10.2147\/OTT.S140671","volume":"10","author":"A Yordanova","year":"2017","unstructured":"Yordanova A, Eppard E, K\u00fcrpig S et al (2017) Theranostics in nuclear medicine practice. Onco Targets Ther 10:4821\u20134828","journal-title":"Onco Targets Ther"},{"key":"9875_CR12","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1038\/s41571-018-0123-y","volume":"16","author":"EGE de Vries","year":"2019","unstructured":"de Vries EGE, Kist de Ruijter L, Lub-de Hooge MN, Dierckx RA, Elias SG, Oosting SF (2019) Integrating molecular nuclear imaging in clinical research to improve anticancer therapy. Nature Rev Clin Oncol 16:241\u2013255","journal-title":"Nature Rev Clin Oncol"},{"key":"9875_CR13","first-page":"75","volume":"85","author":"MR Stacy","year":"2012","unstructured":"Stacy MR, Maxfield MW, Sinusas AJ (2012) Targeted molecular imaging of angiogenesis in PET and SPECT: a review. Yale J Biol Med 85:75\u201386","journal-title":"Yale J Biol Med"},{"key":"9875_CR14","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1007\/s10456-012-9319-4","volume":"16","author":"H Orbay","year":"2013","unstructured":"Orbay H, Hong H, Zhang Y, Cai W (2013) PET\/SPECT imaging of hindlimb ischemia: focusing on angiogenesis and blood flow. Angiogenesis 16:279\u2013287","journal-title":"Angiogenesis"},{"key":"9875_CR15","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1007\/s00259-010-1499-9","volume":"37","author":"I Dijkgraaf","year":"2010","unstructured":"Dijkgraaf I, Boerman OC (2010) Molecular imaging of angiogenesis with SPECT. Eur J Nucl Med Mol Imaging 37:104\u2013113","journal-title":"Eur J Nucl Med Mol Imaging"},{"key":"9875_CR16","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1053\/j.semnuclmed.2016.04.001","volume":"46","author":"JS Eo","year":"2016","unstructured":"Eo JS, Jeong JM (2016) Angiogenesis imaging using 68Ga-RGD PET\/CT: therapeutic implications. Semin Nucl Med 46:419\u2013427","journal-title":"Semin Nucl Med"},{"key":"9875_CR17","doi-asserted-by":"publisher","first-page":"2199","DOI":"10.1021\/bc900167c","volume":"20","author":"S Liu","year":"2009","unstructured":"Liu S (2009) Radiolabeled cyclic RGD peptides as integrin \u03b1v\u03b23-targeted radiotracers: maximizing binding affinity via bivalency. Bioconjug Chem 20:2199\u20132213","journal-title":"Bioconjug Chem"},{"key":"9875_CR18","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1007\/s11307-009-0284-2","volume":"12","author":"S Liu","year":"2010","unstructured":"Liu S, Liu Z, Chen K et al (2010) 18F-Labeled galacto and PEGylated RGD dimers for PET imaging of \u03b1v\u03b23 integrin expression. Mol Imaging Biol 12:530\u2013538","journal-title":"Mol Imaging Biol"},{"key":"9875_CR19","doi-asserted-by":"publisher","first-page":"78","DOI":"10.7150\/thno.13242","volume":"6","author":"H Chen","year":"2016","unstructured":"Chen H, Niu G, Wu H, Chen X (2016) Clinical application of radiolabeled RGD peptides for PET imaging of integrin \u03b1 v \u03b2 3. Theranostics 6:78\u201392","journal-title":"Theranostics"},{"key":"9875_CR20","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.9b01134","author":"Y Gai","year":"2019","unstructured":"Gai Y, Jiang Y, Long Y, Sun L, Liu Q, Qin C, Zhang Y, Zeng D, Lan X (2019) Evaluation of an integrin \u03b1v\u03b23 and aminopeptidase N dual-receptor targeting tracer for breast cancer imaging. Mol Pharmaceutics. https:\/\/doi.org\/10.1021\/acs.molpharmaceut.9b01134","journal-title":"Mol Pharmaceutics"},{"key":"9875_CR21","doi-asserted-by":"publisher","first-page":"109778","DOI":"10.1016\/j.apradiso.2021.109778","volume":"174","author":"N D\u00e9nes","year":"2021","unstructured":"D\u00e9nes N, Kis A, Szab\u00f3 JP et al (2021) In vivo preclinical assessment of novel 68Ga-labelled peptides for imaging of tumor associated angiogenesis using positron emission tomography imaging. Appl Radiat Isot 174:109778","journal-title":"Appl Radiat Isot"},{"key":"9875_CR22","doi-asserted-by":"publisher","first-page":"657","DOI":"10.21873\/invivo.12750","volume":"36","author":"G Farkasinszky","year":"2022","unstructured":"Farkasinszky G, D\u00e9nes N, R\u00e1cz S et al (2022) In vivo imaging of ischemia\/reperfusion-mediated aminopeptidase N expression in surgical rat model using 68Ga-NOTA-c(NGR). In Vivo 36:657\u2013666","journal-title":"In Vivo"},{"key":"9875_CR23","doi-asserted-by":"publisher","first-page":"5544","DOI":"10.3390\/ijms22115544","volume":"22","author":"A Florea","year":"2021","unstructured":"Florea A, Mottaghy FM, Bauwens M (2021) Molecular imaging of angiogenesis in oncology: current preclinical and clinical status. Int J Mol Sci 22:5544","journal-title":"Int J Mol Sci"},{"key":"9875_CR24","doi-asserted-by":"publisher","first-page":"e55589","DOI":"10.7554\/eLife.55589","volume":"9","author":"CS Helker","year":"2020","unstructured":"Helker CS, Eberlein J, Wilhelm K et al (2020) Apelin signaling drives vascular endothelial cells toward a pro-angiogenic state. Elife 9:e55589","journal-title":"Elife"},{"key":"9875_CR25","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.cca.2016.12.023","volume":"466","author":"L Wu","year":"2017","unstructured":"Wu L, Chen L, Li L (2017) Apelin\/APJ system: a novel promising therapy target for pathological angiogenesis. Clin Chim Acta 466:78\u201384","journal-title":"Clin Chim Acta"},{"key":"9875_CR26","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.cca.2018.05.012","volume":"483","author":"H Antushevich","year":"2018","unstructured":"Antushevich H, W\u00f3jcik M (2018) Review: apelin in disease. Clin Chim Acta 483:241\u2013248","journal-title":"Clin Chim Acta"},{"key":"9875_CR27","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1139\/bcb-2014-0072","volume":"92","author":"NA Chapman","year":"2014","unstructured":"Chapman NA, Dupr\u00e9 DJ, Rainey JK (2014) The apelin receptor: physiology, pathology, cell signalling, and ligand modulation of a peptide-activated class A GPCR. Biochem Cell Biol 92:431\u2013440","journal-title":"Biochem Cell Biol"},{"key":"9875_CR28","doi-asserted-by":"crossref","unstructured":"Wysocka MB, Pietraszek-Gremplewicz K, Nowak D (2018) The role of apelin in cardiovascular diseases, obesity and cancer. Front Physiol. https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5974534\/. Accessed 18 Aug 2019","DOI":"10.3389\/fphys.2018.00557"},{"key":"9875_CR29","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1111\/cas.12836","volume":"107","author":"L Zhang","year":"2016","unstructured":"Zhang L, Takara K, Yamakawa D, Kidoya H, Takakura N (2016) Apelin as a marker for monitoring the tumor vessel normalization window during antiangiogenic therapy. Cancer Sci 107:36\u201344","journal-title":"Cancer Sci"},{"key":"9875_CR30","doi-asserted-by":"publisher","first-page":"101697","DOI":"10.18632\/oncotarget.21904","volume":"8","author":"T Chen","year":"2017","unstructured":"Chen T, Liu N, Xu G-M et al (2017) Apelin13\/APJ promotes proliferation of colon carcinoma by activating Notch3 signaling pathway. Oncotarget 8:101697\u2013101706","journal-title":"Oncotarget"},{"key":"9875_CR31","doi-asserted-by":"publisher","first-page":"6020","DOI":"10.1038\/ncomms7020","volume":"6","author":"Q Liu","year":"2015","unstructured":"Liu Q, Hu T, He L et al (2015) Genetic targeting of sprouting angiogenesis using Apln-CreER. Nat Commun 6:6020","journal-title":"Nat Commun"},{"key":"9875_CR32","doi-asserted-by":"publisher","first-page":"2163","DOI":"10.1038\/s41467-020-15729-4","volume":"11","author":"Z Wang","year":"2020","unstructured":"Wang Z, Wang F, Zhong J et al (2020) Using apelin-based synthetic Notch receptors to detect angiogenesis and treat solid tumors. Nat Commun 11:2163","journal-title":"Nat Commun"},{"key":"9875_CR33","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1080\/13543776.2020.1731473","volume":"30","author":"C Fischer","year":"2020","unstructured":"Fischer C (2020) A patent review of apelin receptor (APJR) modulators (2014\u20132019). Expert Opin Ther Pat 30:251\u2013261","journal-title":"Expert Opin Ther Pat"},{"key":"9875_CR34","doi-asserted-by":"crossref","unstructured":"Ason B, Chen Y, Guo Q, et al. (2020) Cardiovascular response to small molecule APJ activation. JCI Insight [Internet]. http:\/\/insight.jci.org\/articles\/view\/132898. Accessed 15 Apr 2020","DOI":"10.1172\/jci.insight.132898"},{"key":"9875_CR35","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1002\/jcp.27047","volume":"234","author":"Z Huang","year":"2019","unstructured":"Huang Z, He L, Chen Z, Chen L (2019) Targeting drugs to APJ receptor: from signaling to pathophysiological effects. J Cell Physiol 234:61\u201374","journal-title":"J Cell Physiol"},{"key":"9875_CR36","doi-asserted-by":"publisher","first-page":"115237","DOI":"10.1016\/j.bmc.2019.115237","volume":"28","author":"S Narayanan","year":"2020","unstructured":"Narayanan S, Vasukuttan V, Rajagopal S, Maitra R, Runyon SP (2020) Identification of potent pyrazole based APELIN receptor (APJ) agonists. Bioorg Med Chem 28:115237","journal-title":"Bioorg Med Chem"},{"key":"9875_CR37","doi-asserted-by":"publisher","first-page":"11454","DOI":"10.1073\/pnas.1812938115","volume":"115","author":"P Garrigue","year":"2018","unstructured":"Garrigue P, Tang J, Ding L et al (2018) Self-assembling supramolecular dendrimer nanosystem for PET imaging of tumors. Proc Natl Acad Sci USA 115:11454\u201311459","journal-title":"Proc Natl Acad Sci USA"},{"key":"9875_CR38","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/j.ejps.2015.05.008","volume":"76","author":"A Andr\u00e9s","year":"2015","unstructured":"Andr\u00e9s A, Ros\u00e9s M, R\u00e0fols C et al (2015) Setup and validation of shake-flask procedures for the determination of partition coefficients (logD) from low drug amounts. Eur J Pharm Sci 76:181\u2013191","journal-title":"Eur J Pharm Sci"},{"key":"9875_CR39","doi-asserted-by":"publisher","first-page":"e30004V10","DOI":"10.1371\/journal.pbio.3000410","volume":"18","author":"N Percie du Sert","year":"2020","unstructured":"Percie du Sert N, Hurst V, Ahluwalia A et al (2020) The ARRIVE guidelines 2.0: updated guidelines for reporting animal research. PLoS Biol 18:e30004V10","journal-title":"PLoS Biol"},{"key":"9875_CR40","doi-asserted-by":"publisher","first-page":"121","DOI":"10.3390\/pharmaceutics14010121","volume":"14","author":"R Giraud","year":"2022","unstructured":"Giraud R, Moyon A, Simoncini S et al (2022) Tracking radiolabeled endothelial microvesicles predicts their therapeutic efficacy: a proof-of-concept study in peripheral ischemia mouse model using SPECT\/CT imaging. Pharmaceutics 14:121","journal-title":"Pharmaceutics"},{"key":"9875_CR41","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2017.00092","author":"P Yang","year":"2017","unstructured":"Yang P, Kuc RE, Brame AL et al (2017) [Pyr1]Apelin-13(1\u201312) is a biologically active ACE2 metabolite of the endogenous cardiovascular peptide [Pyr1]Apelin-13. Front Neurosci. https:\/\/doi.org\/10.3389\/fnins.2017.00092","journal-title":"Front Neurosci"},{"key":"9875_CR42","doi-asserted-by":"crossref","unstructured":"Feng M, Yao G, Yu H, Qing Y, Wang K. (2016) Tumor apelin, not serum apelin, is associated with the clinical features and prognosis of gastric cancer. BMC Cancer, vol 16. https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5062883\/. Accessed 29 Jan 2020","DOI":"10.1186\/s12885-016-2815-y"},{"key":"9875_CR43","doi-asserted-by":"crossref","unstructured":"Podg\u00f3rska M, Diakowska D, Pietraszek-Gremplewicz K, Nienartowicz M, Nowak D. (2019) Evaluation of Apelin and Apelin Receptor Level in the Primary Tumor and Serum of Colorectal Cancer Patients. J Clin Med, vol 8. https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6832595\/. Accessed 29 Jan 2020","DOI":"10.3390\/jcm8101513"},{"key":"9875_CR44","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1139\/cjpp-2019-0502","volume":"98","author":"\u0130 Birsen","year":"2020","unstructured":"Birsen \u0130, \u0130zg\u00fct-Uysal VN, Soylu H, \u00dcst\u00fcnel \u0130 (2020) The effect of apelin-13 on gastric ischemia\/reperfusion injury: the roles of sensory nerves and vagus nerve. Can J Physiol Pharmacol 98:282\u2013295","journal-title":"Can J Physiol Pharmacol"},{"key":"9875_CR45","doi-asserted-by":"publisher","first-page":"H2308","DOI":"10.1152\/ajpheart.01177.2010","volume":"300","author":"R Rastaldo","year":"2011","unstructured":"Rastaldo R, Cappello S, Folino A et al (2011) Apelin-13 limits infarct size and improves cardiac postischemic mechanical recovery only if given after ischemia. Am J Physiol Heart Circ Physiol 300:H2308-2315","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"9875_CR46","doi-asserted-by":"publisher","first-page":"1349","DOI":"10.1038\/s41598-020-58452-2","volume":"10","author":"S Yamazaki","year":"2020","unstructured":"Yamazaki S, Sekiguchi A, Uchiyama A et al (2020) Apelin\/APJ signaling suppresses the pressure ulcer formation in cutaneous ischemia-reperfusion injury mouse model. Sci Rep 10:1349","journal-title":"Sci Rep"}],"container-title":["Angiogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10456-023-09875-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10456-023-09875-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10456-023-09875-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T10:20:29Z","timestamp":1688725229000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10456-023-09875-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,27]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["9875"],"URL":"https:\/\/doi.org\/10.1007\/s10456-023-09875-8","relation":{},"ISSN":["0969-6970","1573-7209"],"issn-type":[{"value":"0969-6970","type":"print"},{"value":"1573-7209","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,27]]},"assertion":[{"value":"5 January 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 March 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 March 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors have no relevant financial or non-financial interests to disclose.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"All procedures involving animals were in compliance with the European Community Council 2010\/63\/EU Directive and the ARRIVE guidelines 2.0. Ethical approval was granted by the Institution\u2019s Animal Care and Use Committee (CE71, Aix-Marseille Universit\u00e9, projects #15790, #32157, #31843).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}