{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:37:36Z","timestamp":1790098656026,"version":"4.0.1"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY031039"],"award-info":[{"award-number":["R01EY031039"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY031039"],"award-info":[{"award-number":["R01EY031039"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01AG082108"],"award-info":[{"award-number":["R01AG082108"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01AG082108"],"award-info":[{"award-number":["R01AG082108"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL164592"],"award-info":[{"award-number":["R01HL164592"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Angiogenesis"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inflammasome activation is implicated in diseases of aberrant angiogenesis such as age-related macular degeneration (AMD), though its precise role in choroidal neovascularization (CNV), a characteristic pathology of advanced AMD, is ill-defined. Reports on inhibition of inflammasome constituents on CNV are variable and the precise role of inflammasome in mediating pathological angiogenesis is unclear. Historically, subretinal injection of inflammasome agonists alone has been used to investigate retinal pigmented epithelium (RPE) degeneration, while the laser photocoagulation model has been used to study pathological angiogenesis in a model of CNV. Here, we report that the simultaneous introduction of any of several disease-relevant inflammasome agonists (<jats:italic>Alu<\/jats:italic> or B2 RNA, <jats:italic>Alu<\/jats:italic> cDNA, or oligomerized amyloid \u03b2 (1\u201340)) exacerbates laser-induced CNV. These activities were diminished or abrogated by genetic or pharmacological targeting of inflammasome signaling constituents including P2rx7, Nlrp3, caspase-1, caspase-11, and Myd88, as well as in myeloid-specific caspase-1 knockout mice. <jats:italic>Alu<\/jats:italic> RNA treatment induced inflammasome activation in macrophages within the CNV lesion, and increased accumulation of macrophages in an inflammasome-dependent manner. Finally, IL-1\u03b2 neutralization prevented inflammasome agonist-induced chemotaxis, macrophage trafficking, and angiogenesis. Collectively, these observations support a model wherein inflammasome stimulation promotes and exacerbates CNV and may be a therapeutic target for diseases of angiogenesis such as neovascular AMD.<\/jats:p>","DOI":"10.1007\/s10456-024-09949-1","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T08:02:08Z","timestamp":1727164928000},"page":"919-929","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Inflammasome activation aggravates choroidal neovascularization"],"prefix":"10.1007","volume":"27","author":[{"given":"Ryan D.","family":"Makin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Apicella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roshni","family":"Dholkawala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Fukuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichiro","family":"Hirahara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshio","family":"Hirano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Younghee","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayami","family":"Nagasaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Nagasaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siddharth","family":"Narendran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akhil","family":"Varshney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-bin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayakrishna","family":"Ambati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley D.","family":"Gelfand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,24]]},"reference":[{"issue":"34","key":"9949_CR1","doi-asserted-by":"publisher","first-page":"13781","DOI":"10.1073\/pnas.1206494109","volume":"109","author":"S Dridi","year":"2012","unstructured":"Dridi S, Hirano Y, Tarallo V, Kim Y, Fowler BJ, Ambati BK et al (2012) ERK1\/2 activation is a therapeutic target in age-related macular degeneration. Proc Natl Acad Sci 109(34):13781\u201313786","journal-title":"Proc Natl Acad Sci"},{"issue":"4","key":"9949_CR2","doi-asserted-by":"publisher","first-page":"847","DOI":"10.1016\/j.cell.2012.03.036","volume":"149","author":"V Tarallo","year":"2012","unstructured":"Tarallo V, Hirano Y, Gelfand BD, Dridi S, Kerur N, Kim Y et al (2012) DICER1 loss and Alu RNA induce age-related macular degeneration via the NLRP3 inflammasome and MyD88. Cell 149(4):847\u2013859","journal-title":"Cell"},{"issue":"11","key":"9949_CR3","doi-asserted-by":"publisher","first-page":"1686","DOI":"10.1016\/j.celrep.2015.05.023","volume":"11","author":"BD Gelfand","year":"2015","unstructured":"Gelfand BD, Wright CB, Kim Y, Yasuma T, Yasuma R, Li S et al (2015) Iron Toxicity in the retina requires Alu RNA and the NLRP3 inflammasome. Cell Rep 11(11):1686\u20131693","journal-title":"Cell Rep"},{"issue":"45","key":"9949_CR4","doi-asserted-by":"publisher","first-page":"16082","DOI":"10.1073\/pnas.1403814111","volume":"111","author":"Y Kim","year":"2014","unstructured":"Kim Y, Tarallo V, Kerur N, Yasuma T, Gelfand BD, Bastos-Carvalho A et al (2014) DICER1\/Alu RNA dysmetabolism induces caspase-8-mediated cell death in age-related macular degeneration. Proc Natl Acad Sci U S A 111(45):16082\u201316087","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"9949_CR5","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1016\/j.celrep.2013.08.002","volume":"4","author":"AG Marneros","year":"2013","unstructured":"Marneros AG (2013) NLRP3 inflammasome blockade inhibits VEGF-A-induced age-related macular degeneration. Cell Rep 4(5):945\u2013958","journal-title":"Cell Rep"},{"issue":"5","key":"9949_CR6","doi-asserted-by":"publisher","first-page":"2579","DOI":"10.1073\/pnas.1909761117","volume":"117","author":"CB Wright","year":"2020","unstructured":"Wright CB, Uehara H, Kim Y, Yasuma T, Yasuma R, Hirahara S et al (2020) Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice. Proc Natl Acad Sci U S A 117(5):2579\u20132587","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"9949_CR7","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1038\/nm.2717","volume":"18","author":"SL Doyle","year":"2012","unstructured":"Doyle SL, Campbell M, Ozaki E, Salomon RG, Mori A, Kenna PF et al (2012) NLRP3 has a protective role in age-related macular degeneration through the induction of IL-18 by drusen components. Nat Med 18(5):791\u2013798","journal-title":"Nat Med"},{"issue":"12","key":"9949_CR8","doi-asserted-by":"publisher","first-page":"1372","DOI":"10.1038\/nm.3671","volume":"20","author":"Y Hirano","year":"2014","unstructured":"Hirano Y, Yasuma T, Mizutani T, Fowler BJ, Tarallo V, Yasuma R et al (2014) IL-18 is not therapeutic for neovascular age-related macular degeneration. Nat Med 20(12):1372\u20131375","journal-title":"Nat Med"},{"issue":"7371","key":"9949_CR9","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1038\/nature10558","volume":"479","author":"N Kayagaki","year":"2011","unstructured":"Kayagaki N, Warming S, Lamkanfi M, Vande Walle L, Louie S, Dong J et al (2011) Non-canonical inflammasome activation targets caspase-11. Nature 479(7371):117\u2013121","journal-title":"Nature"},{"issue":"7081","key":"9949_CR10","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1038\/nature04517","volume":"440","author":"TD Kanneganti","year":"2006","unstructured":"Kanneganti TD, \u00d6z\u00f6ren N, Body-Malapel M, Amer A, Park JH, Franchi L et al (2006) Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin\/Nalp3. Nature 440(7081):233\u2013236","journal-title":"Nature"},{"issue":"6","key":"9949_CR11","doi-asserted-by":"publisher","first-page":"1468","DOI":"10.1038\/s41596-022-00689-4","volume":"17","author":"P Huang","year":"2022","unstructured":"Huang P, Narendran S, Pereira F, Fukuda S, Nagasaka Y, Apicella I et al (2022) Subretinal injection in mice to study retinal physiology and disease. Nat Protoc 17(6):1468\u20131485","journal-title":"Nat Protoc"},{"issue":"66","key":"9949_CR12","doi-asserted-by":"publisher","first-page":"eabi4493","DOI":"10.1126\/sciimmunol.abi4493","volume":"6","author":"SB Wang","year":"2021","unstructured":"Wang SB, Narendran S, Hirahara S, Varshney A, Pereira F, Apicella I et al (2021) DDX17 is an essential mediator of sterile NLRC4 inflammasome activation by retrotransposon RNAs. Sci Immunol 6(66):eabi4493","journal-title":"Sci Immunol"},{"issue":"7338","key":"9949_CR13","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1038\/nature09830","volume":"471","author":"H Kaneko","year":"2011","unstructured":"Kaneko H, Dridi S, Tarallo V, Gelfand BD, Fowler BJ, Cho WG et al (2011) DICER1 deficit induces Alu RNA toxicity in age-related macular degeneration. Nature 471(7338):325\u2013330","journal-title":"Nature"},{"issue":"1","key":"9949_CR14","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1038\/s41392-021-00537-z","volume":"6","author":"S Narendran","year":"2021","unstructured":"Narendran S, Pereira F, Yerramothu P, Apicella I, Wang SB, Ambati K et al (2021) Nucleoside reverse transcriptase inhibitors and Kamuvudines inhibit amyloid-\u03b2 induced retinal pigmented epithelium degeneration. Signal Transduct Target Ther 6(1):149","journal-title":"Signal Transduct Target Ther"},{"issue":"10","key":"9949_CR15","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1167\/iovs.61.10.4","volume":"61","author":"S Narendran","year":"2020","unstructured":"Narendran S, Pereira F, Yerramothu P, Apicella I, bin Wang S, Varshney A et al (2020) A clinical metabolite of Azidothymidine inhibits experimental Choroidal neovascularization and retinal pigmented epithelium degeneration. Invest Ophthalmol Vis Sci 61(10):4","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"9949_CR16","doi-asserted-by":"crossref","unstructured":"Fukuda S, Varshney A, Fowler BJ, Wang SB, Narendran S, Ambati K et al (2021) Cytoplasmic synthesis of endogenous Alu complementary DNA via reverse transcription and implications in age-related macular degeneration. Proc Natl Acad Sci U S A. ;118(6)","DOI":"10.1073\/pnas.2022751118"},{"key":"9949_CR17","doi-asserted-by":"crossref","unstructured":"Fukuda S, Narendran S, Varshney A, Nagasaka Y, Wang S bin, Ambati K et al (2022) Alu complementary DNA is enriched in atrophic macular degeneration and triggers retinal pigmented epithelium toxicity via cytosolic innate immunity. Sci Adv. ;7(40):eabj3658","DOI":"10.1126\/sciadv.abj3658"},{"issue":"2","key":"9949_CR18","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1172\/JCI25843","volume":"116","author":"V Luibl","year":"2006","unstructured":"Luibl V, Isas JM, Kayed R, Glabe CG, Langen R, Chen J (2006) Drusen deposits associated with aging and age-related macular degeneration contain nonfibrillar amyloid oligomers. J Clin Invest 116(2):378\u2013385","journal-title":"J Clin Invest"},{"issue":"3","key":"9949_CR19","doi-asserted-by":"publisher","first-page":"1304","DOI":"10.1167\/iovs.09-4207","volume":"51","author":"JM Isas","year":"2010","unstructured":"Isas JM, Luibl V, Johnson LV, Kayed R, Wetzel R, Glabe CG et al (2010) Soluble and mature amyloid fibrils in Drusen deposits. Invest Ophthalmol Vis Sci 51(3):1304\u20131310","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"4","key":"9949_CR20","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.preteyeres.2011.02.004","volume":"30","author":"K Ohno-Matsui","year":"2011","unstructured":"Ohno-Matsui K (2011) Parallel findings in age-related macular degeneration and Alzheimer\u2019s disease. Prog Retin Eye Res 30(4):217\u2013238","journal-title":"Prog Retin Eye Res"},{"issue":"33","key":"9949_CR21","doi-asserted-by":"publisher","first-page":"11900","DOI":"10.1073\/pnas.0503015102","volume":"102","author":"G Malek","year":"2005","unstructured":"Malek G, Johnson LV, Mace BE, Saloupis P, Schmechel DE, Rickman DW et al (2005) Apolipoprotein E allele-dependent pathogenesis: a model for age-related retinal degeneration. Proc Natl Acad Sci 102(33):11900\u201311905","journal-title":"Proc Natl Acad Sci"},{"issue":"28","key":"9949_CR22","doi-asserted-by":"publisher","first-page":"E279","DOI":"10.1073\/pnas.1100901108","volume":"108","author":"JD Ding","year":"2011","unstructured":"Ding JD, Johnson LV, Herrmann R, Farsiu S, Smith SG, Groelle M et al (2011) Anti-amyloid therapy protects against retinal pigmented epithelium damage and vision loss in a model of age-related macular degeneration. Proc Natl Acad Sci U S A 108(28):E279\u2013E287","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"12","key":"9949_CR23","doi-asserted-by":"publisher","first-page":"7395","DOI":"10.1167\/iovs.13-12500","volume":"54","author":"N Kerur","year":"2013","unstructured":"Kerur N, Hirano Y, Tarallo V, Fowler BJ, Bastos-Carvalho A, Yasuma T et al (2013) TLR-independent and P2X7-dependent signaling mediate Alu RNA-induced NLRP3 inflammasome activation in geographic atrophy. Invest Ophthalmol Vis Sci 54(12):7395\u20137401","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"6212","key":"9949_CR24","doi-asserted-by":"publisher","first-page":"1000","DOI":"10.1126\/science.1261754","volume":"346","author":"BJ Fowler","year":"2014","unstructured":"Fowler BJ, Gelfand BD, Kim Y, Kerur N, Tarallo V, Hirano Y et al (2014) Nucleoside reverse transcriptase inhibitors possess intrinsic anti-inflammatory activity. Science 346(6212):1000\u20131003","journal-title":"Science"},{"key":"9949_CR25","unstructured":"Benjamin F. Compositions and methods for treating retinal degradation. Us Jayakrishnaa (2021) Univ Kentucky Res Found OP - US 202017116755 A OP - US 201916361810 A OP - US 201615142087 A OP - US 201414450000 A OP - US 201361861290 P OP - US 201461987612 P"},{"issue":"1","key":"9949_CR26","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1038\/nm.4450","volume":"24","author":"N Kerur","year":"2018","unstructured":"Kerur N, Fukuda S, Banerjee D, Kim Y, Fu D, Apicella I et al (2018) cGAS drives noncanonical-inflammasome activation in age-related macular degeneration. Nat Med 24(1):50\u201361","journal-title":"Nat Med"},{"issue":"1","key":"9949_CR27","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/S1074-7613(00)80596-8","volume":"9","author":"O Adachi","year":"1998","unstructured":"Adachi O, Kawai T, Takeda K, Matsumoto M, Tsutsui H, Sakagami M et al (1998) Targeted disruption of the MyD88 gene results in loss of IL-1- and IL-18-mediated function. Immunity 9(1):143\u2013150","journal-title":"Immunity"},{"issue":"16","key":"9949_CR28","doi-asserted-by":"publisher","first-page":"15809","DOI":"10.1074\/jbc.C400613200","volume":"280","author":"M Loiarro","year":"2005","unstructured":"Loiarro M, Sette C, Gallo G, Ciacci A, Fant\u00f2 N, Mastroianni D et al (2005) Peptide-mediated interference of TIR domain dimerization in MyD88 inhibits interleukin-1-dependent activation of NF-\u03baB. J Biol Chem 280(16):15809\u201315814","journal-title":"J Biol Chem"},{"key":"9949_CR29","doi-asserted-by":"crossref","unstructured":"Sakurai E, Anand A, Ambati BK, Van Rooijen N, Ambati J (2003) Macrophage depletion inhibits experimental choroidal neovascularization. Invest Ophthalmol Vis Sci","DOI":"10.1167\/iovs.03-0097"},{"issue":"12","key":"9949_CR30","doi-asserted-by":"publisher","first-page":"7439","DOI":"10.1167\/iovs.13-12546","volume":"54","author":"DG Espinosa-Heidmann","year":"2013","unstructured":"Espinosa-Heidmann DG, Malek G, Mettu PS, Caicedo A, Saloupis P, Gach S et al (2013) Bone marrow transplantation transfers age-related susceptibility to Neovascular Remodeling in Murine Laser-Induced Choroidal Neovascularization. Invest Ophthalmol Vis Sci 54(12):7439\u20137449","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"7","key":"9949_CR31","first-page":"4705","volume":"183","author":"Y Carmi","year":"2009","unstructured":"Carmi Y, Voronov E, Dotan S, Lahat N, Rahat MA, Fogel M et al (2009) The role of macrophage-derived IL-1 in induction and maintenance of angiogenesis. J Immunol Baltim Md 1950 183(7):4705\u20134714","journal-title":"J Immunol Baltim Md 1950"},{"issue":"7","key":"9949_CR32","doi-asserted-by":"publisher","first-page":"e69552","DOI":"10.1371\/journal.pone.0069552","volume":"8","author":"Z Shao","year":"2013","unstructured":"Shao Z, Friedlander M, Hurst CG, Cui Z, Pei DT, Evans LP et al (2013) Choroid sprouting assay: an Ex vivo model of Microvascular Angiogenesis. PLoS ONE 8(7):e69552","journal-title":"PLoS ONE"},{"issue":"15","key":"9949_CR33","doi-asserted-by":"publisher","first-page":"5059","DOI":"10.1167\/iovs.19-28165","volume":"60","author":"S Droho","year":"2019","unstructured":"Droho S, Cuda CM, Perlman H, Lavine JA (2019) Monocyte-derived macrophages are necessary for Beta-adrenergic receptor-driven Choroidal Neovascularization Inhibition. Invest Ophthalmol Vis Sci 60(15):5059\u20135069","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"9949_CR34","doi-asserted-by":"crossref","unstructured":"Wright CB, Uehara H, Kim Y, Yasuma T, Yasuma R, Hirahara S et al (2020) Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice. Proc Natl Acad Sci U S A","DOI":"10.1073\/pnas.1909761117"},{"key":"9949_CR35","doi-asserted-by":"crossref","unstructured":"Malsy J, Alvarado AC, Lamontagne JO, Strittmatter K, Marneros AG (2020) Distinct effects of complement and of NLRP3- and non-NLRP3 inflammasomes for choroidal neovascularization. Saban DR, Rothlin C V, editors. eLife. ;9:e60194","DOI":"10.7554\/eLife.60194"},{"issue":"2","key":"9949_CR36","doi-asserted-by":"publisher","first-page":"614","DOI":"10.1016\/j.bbrc.2018.11.125","volume":"508","author":"S Vogel","year":"2019","unstructured":"Vogel S, Murthy P, Cui X, Lotze MT, Zeh HJ, Sachdev U (2019) TLR4-dependent upregulation of the platelet NLRP3 inflammasome promotes platelet aggregation in a murine model of hindlimb ischemia. Biochem Biophys Res Commun 508(2):614\u2013619","journal-title":"Biochem Biophys Res Commun"},{"issue":"5","key":"9949_CR37","doi-asserted-by":"publisher","first-page":"1710","DOI":"10.1161\/ATVBAHA.121.315805","volume":"41","author":"Y Ma","year":"2021","unstructured":"Ma Y, Jia L, Wang Y, Ji Y, Chen J, Ma H et al (2021) Heme Oxygenase-1 in Macrophages impairs the Perfusion Recovery after Hindlimb Ischemia by suppressing autolysosome-dependent degradation of NLRP3. Arterioscler Thromb Vasc Biol 41(5):1710\u20131723","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"5","key":"9949_CR38","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1189\/jlb.3HI0715-321R","volume":"99","author":"F Gimenez","year":"2016","unstructured":"Gimenez F, Bhela S, Dogra P, Harvey L, Varanasi SK, Jaggi U et al (2016) The inflammasome NLRP3 plays a protective role against a viral immunopathological lesion. J Leukoc Biol 99(5):647\u2013657","journal-title":"J Leukoc Biol"},{"issue":"8","key":"9949_CR39","doi-asserted-by":"publisher","first-page":"803","DOI":"10.1111\/aos.13427","volume":"95","author":"S Loukovaara","year":"2017","unstructured":"Loukovaara S, Piippo N, Kinnunen K, Hytti M, Kaarniranta K, Kauppinen A (2017) NLRP3 inflammasome activation is associated with proliferative diabetic retinopathy. Acta Ophthalmol (Copenh) 95(8):803\u2013808","journal-title":"Acta Ophthalmol (Copenh)"},{"issue":"1","key":"9949_CR40","doi-asserted-by":"publisher","first-page":"4737","DOI":"10.1038\/s41467-020-18528-z","volume":"11","author":"J Ambati","year":"2020","unstructured":"Ambati J, Magagnoli J, Leung H, Wang SB, Andrews CA, Fu D et al (2020) Repurposing anti-inflammasome NRTIs for improving insulin sensitivity and reducing type 2 diabetes development. Nat Commun 11(1):4737","journal-title":"Nat Commun"}],"container-title":["Angiogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10456-024-09949-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10456-024-09949-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10456-024-09949-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T13:25:32Z","timestamp":1731590732000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10456-024-09949-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,24]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["9949"],"URL":"https:\/\/doi.org\/10.1007\/s10456-024-09949-1","relation":{},"ISSN":["0969-6970","1573-7209"],"issn-type":[{"value":"0969-6970","type":"print"},{"value":"1573-7209","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,24]]},"assertion":[{"value":"23 February 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 September 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 September 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"J.A: co-founder of DiceRx, iVeena Holdings, iVeena Delivery Systems and Inflammasome Therapeutics, and, unrelated to this work, has been a consultant for Abbvie\/Allergan, Boehringer-Ingelheim, Janssen, Olix Pharmaceuticals, Retinal Solutions, and Saksin LifeSciences. B.D.G: co-founder of DiceRx. J.A., S.W., B.D.G.: inventors on matter-related patent applications filed by the University of Virginia or Kentucky.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}