{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T16:07:44Z","timestamp":1781280464225,"version":"3.54.1"},"reference-count":5,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100017804","name":"City of Hope","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100017804","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Angiogenesis"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Endothelial cells (ECs) are crucial in cancer development and progression, partly by regulating tumor angiogenesis and immune modulation. As a key component of the RNA-induced silencing complex, Argonaute 1 (AGO1) regulates tumor biology, yet the specific function of AGO1 within ECs in the tumor microenvironment remains undefined. Here, we investigated the effects of endothelial-specific AGO1 knockout (EC-AGO1-KO) on tumor vascularization and immune regulation in a mouse syngeneic breast cancer model induced by E0771 cells. EC-AGO1-KO mice exhibited significantly reduced tumor burden compared to their wild-type (WT) littermates, accompanied by reduced vascularization and enhanced immune cell infiltration. Histological and single-cell RNA sequencing analyses revealed increased infiltration of CD8\u207a T cells and macrophages in EC-AGO1-KO tumors, indicative of an immunostimulatory microenvironment. In vitro, AGO1 knockdown in mouse ECs co-cultured with E0771 tumor cells led to higher levels of\n                    <jats:italic>Cxcl10<\/jats:italic>\n                    and\n                    <jats:italic>Vcam1<\/jats:italic>\n                    expression, suggesting a pro-inflammatory and leukocyte-recruiting effect. Together, these findings identify endothelial AGO1 as a key regulator of tumor vasculature and immune homeostasis in breast cancer, suggesting that targeting endothelial AGO1 may represent a novel therapeutic strategy to modulate tumor vasculature while enhancing anti-tumor immunity.\n                  <\/jats:p>","DOI":"10.1007\/s10456-026-10048-6","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T15:44:59Z","timestamp":1781279099000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Endothelial AGO1 deficiency reduces breast cancer burden in mice"],"prefix":"10.1007","volume":"29","author":[{"given":"Xuejing","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alonso","family":"Tapia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongqiang","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingjun","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naseeb Kaur","family":"Malhi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muxi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwan Ho","family":"Law","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamad","family":"Haidar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Skylar","family":"Giacobetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawei","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saul J.","family":"Priceman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen Bouman","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,12]]},"reference":[{"issue":"10","key":"10048_CR1","doi-asserted-by":"publisher","first-page":"1187","DOI":"10.1101\/gad.1201404","volume":"18","author":"H Vaucheret","year":"2004","unstructured":"Vaucheret H, Vazquez F, Crete P, Bartel DP (2004) The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development. Genes Dev 18(10):1187\u20131197. https:\/\/doi.org\/10.1101\/gad.1201404","journal-title":"Genes Dev"},{"key":"10048_CR2","doi-asserted-by":"publisher","unstructured":"Nowak I, Sarshad AA (2021) Argonaute proteins take center stage in cancers. Cancers (Basel) 13(4). https:\/\/doi.org\/10.3390\/cancers13040788","DOI":"10.3390\/cancers13040788"},{"issue":"3","key":"10048_CR3","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1172\/JCI65344","volume":"123","author":"Z Chen","year":"2013","unstructured":"Chen Z, Lai TC, Jan YH, Lin FM, Wang WC, Xiao H, Wang YT, Sun W, Cui X, Li YS, Fang T, Zhao H, Padmanabhan C, Sun R, Wang DL, Jin H, Chau GY, Huang HD, Hsiao M, Shyy JY (2013) Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis. J Clin Invest 123(3):1057\u20131067. https:\/\/doi.org\/10.1172\/JCI65344","journal-title":"J Clin Invest"},{"key":"10048_CR4","doi-asserted-by":"publisher","unstructured":"Liu X, Yuan D, Luo Y, Tang X, Tapia A, Malhi NK, Chadha RS, Tiwari S, Swiderski P, Wang KC, Kortylewski M, Pardi N, Wei L, Huang W, Chen ZB (2025) Endothelial AGO1 drives vascular inflammation and atherosclerosis via a non-canonical nuclear mechanism. bioRxiv. https:\/\/doi.org\/10.1101\/2025.05.01.651783","DOI":"10.1101\/2025.05.01.651783"},{"issue":"4","key":"10048_CR5","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1161\/CIRCULATIONAHA.119.041231","volume":"142","author":"X Tang","year":"2020","unstructured":"Tang X, Miao Y, Luo Y, Sriram K, Qi Z, Lin FM, Gu Y, Lai CH, Hsu CY, Peterson KL, Van Keuren-Jensen K, Fueger PT, Yeo GW, Natarajan R, Zhong S, Chen ZB (2020) Suppression of endothelial AGO1 promotes adipose tissue browning and improves metabolic dysfunction. Circulation 142(4):365\u2013379. https:\/\/doi.org\/10.1161\/CIRCULATIONAHA.119.041231","journal-title":"Circulation"}],"container-title":["Angiogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10456-026-10048-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10456-026-10048-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10456-026-10048-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T15:44:59Z","timestamp":1781279099000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10456-026-10048-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,12]]},"references-count":5,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["10048"],"URL":"https:\/\/doi.org\/10.1007\/s10456-026-10048-6","relation":{},"ISSN":["1573-7209"],"issn-type":[{"value":"1573-7209","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,12]]},"assertion":[{"value":"5 November 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 April 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 June 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"43"}}