{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T02:18:32Z","timestamp":1789870712283,"version":"4.0.1"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2017,1,2]],"date-time":"2017-01-02T00:00:00Z","timestamp":1483315200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31372564"],"award-info":[{"award-number":["31372564"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31300005"],"award-info":[{"award-number":["31300005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31302166"],"award-info":[{"award-number":["31302166"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Research Foundation for Advanced Talents of Qingdao Agricultural University","award":["6631113310"],"award-info":[{"award-number":["6631113310"]}]},{"name":"Research Foundation for Advanced Talents of Qingdao Agricultural University","award":["6631113308"],"award-info":[{"award-number":["6631113308"]}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2013CM028"],"award-info":[{"award-number":["ZR2013CM028"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Antonie van Leeuwenhoek"],"published-print":{"date-parts":[[2017,4]]},"DOI":"10.1007\/s10482-016-0827-x","type":"journal-article","created":{"date-parts":[[2017,1,2]],"date-time":"2017-01-02T15:18:48Z","timestamp":1483370328000},"page":"607-614","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Development of a regulatable expression system for the functional study of Vibrio vulnificus essential genes"],"prefix":"10.1007","volume":"110","author":[{"given":"Yan-Jiao","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guozhong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyuan","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baozhi","family":"Kuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2616-4367","authenticated-orcid":false,"given":"Shiyong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,1,2]]},"reference":[{"key":"827_CR1","doi-asserted-by":"publisher","first-page":"17593","DOI":"10.1038\/srep17593","volume":"5","author":"R Fern\u00e1ndez-Pi\u00f1ar","year":"2015","unstructured":"Fern\u00e1ndez-Pi\u00f1ar R, Sciuto AL, Rossi A, Ranucci S, Bragonzi A, Imperi F (2015) In vitro and in vivo screening for novel essential cell-envelope proteins in Pseudomonas aeruginosa. Sci Rep 5:17593","journal-title":"Sci Rep"},{"key":"827_CR2","doi-asserted-by":"publisher","first-page":"4121","DOI":"10.1128\/jb.177.14.4121-4130.1995","volume":"177","author":"L Guzman","year":"1995","unstructured":"Guzman L, Dominique B, Michael JC, Beckwith J (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose P BAD promoter. J Bacteriol 177:4121\u20134130","journal-title":"J Bacteriol"},{"key":"827_CR20","doi-asserted-by":"publisher","first-page":"1723","DOI":"10.1128\/IAI.01046-08","volume":"77","author":"MK Jones","year":"2009","unstructured":"Jones MK, Oliver JD (2009) Vibrio vulnificus: disease and pathogenesis. Infect Immun 77:1723\u20131733","journal-title":"Infect Immun"},{"key":"827_CR3","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1186\/s12866-015-0418-4","volume":"15","author":"MJ Kim","year":"2015","unstructured":"Kim MJ, Kim J, Lee HY, Noh HJ, Lee KH, Park SJ (2015) Role of AcsR in expression of the acetyl-CoA synthetase gene in Vibrio vulnificus. BMC Microbiol 15:86","journal-title":"BMC Microbiol"},{"key":"827_CR4","doi-asserted-by":"publisher","first-page":"1477","DOI":"10.1128\/AEM.03720-13","volume":"80","author":"K Klevanskaa","year":"2014","unstructured":"Klevanskaa K, Bier N, Stingl K, Strauch E, Hertwig S (2014) PVv3, a new shuttle vector for gene expression in Vibrio vulnificus. Appl Environ Microbiol 80:1477\u20131481","journal-title":"Appl Environ Microbiol"},{"key":"827_CR5","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1128\/IAI.00682-10","volume":"79","author":"A Kovacs-Simon","year":"2011","unstructured":"Kovacs-Simon A, Titball RW, Michell SL (2011) Lipoproteins of bacterial pathogens. Infect Immun 79:548\u2013561","journal-title":"Infect Immun"},{"key":"827_CR6","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1128\/IAI.01141-13","volume":"82","author":"JG Lim","year":"2014","unstructured":"Lim JG, Choi SH (2014) IscR is a global regulator essential for pathogenesis of Vibrio vulnificus and induced by host cells. Infect Immun 82:569\u2013578","journal-title":"Infect Immun"},{"key":"827_CR7","doi-asserted-by":"publisher","first-page":"2575","DOI":"10.1128\/jb.170.6.2575-2583.1988","volume":"170","author":"VL Miller","year":"1988","unstructured":"Miller VL, Mekalanos JJ (1988) A novel suicide vector and its use in construction of insertion mutations: osmo regulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol 170:2575\u20132583","journal-title":"J Bacteriol"},{"key":"827_CR8","doi-asserted-by":"publisher","first-page":"1310","DOI":"10.1128\/jb.178.5.1310-1319.1996","volume":"178","author":"DL Milton","year":"1996","unstructured":"Milton DL, O\u2019Toole R, H\u00f6rstedt P, Wolf-Watz H (1996) Flagellin A is essential for the virulence of Vibrio anguillarum. J Bacteriol 178:1310\u20131319","journal-title":"J Bacteriol"},{"key":"827_CR9","doi-asserted-by":"publisher","first-page":"4247","DOI":"10.1111\/febs.12041","volume":"279","author":"H Nakayama","year":"2012","unstructured":"Nakayama H, Kurokawa K, Lee BL (2012) Lipoproteins in bacteria: structures and biosynthetic pathways. FEBS J 279:4247\u20134268","journal-title":"FEBS J"},{"key":"827_CR10","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1146\/annurev-micro-090110-102859","volume":"65","author":"S Okuda","year":"2011","unstructured":"Okuda S, Tokuda H (2011) Lipoprotein sorting in bacteria. Annu Rev Microbiol 65:239\u2013259","journal-title":"Annu Rev Microbiol"},{"key":"827_CR11","first-page":"253","volume-title":"Oceans and health: pathogens in the marine environment","author":"JD Oliver","year":"2006","unstructured":"Oliver JD (2006) Vibrio vulnificus. In: Belkin S, Colwell RR (eds) Oceans and health: pathogens in the marine environment. Springer, New York, pp 253\u2013276"},{"key":"827_CR12","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1007\/s00248-012-0140-9","volume":"65","author":"JD Oliver","year":"2013","unstructured":"Oliver JD (2013) Vibrio vulnificus: death on the half shell. A personal journey with the pathogen and its ecology. Microb Ecol 65:793\u2013799","journal-title":"Microb Ecol"},{"key":"827_CR13","doi-asserted-by":"publisher","unstructured":"Oliver JD (2015) The biology of Vibrio vulnificus. Microbiol Spectr 3:VE-0001-2014","DOI":"10.1128\/microbiolspec.VE-0001-2014"},{"key":"827_CR14","doi-asserted-by":"publisher","first-page":"2062","DOI":"10.1128\/JB.00110-11","volume":"193","author":"JH Park","year":"2011","unstructured":"Park JH, Cho YJ, Chun J, Seok YJ, Lee JK, Kim KS, Lee KH, Park SJ, Choi SH (2011) Complete genome sequence of Vibrio vulnificus MO6-24\/O. J Bacteriol 193:2062\u20132063","journal-title":"J Bacteriol"},{"key":"827_CR15","doi-asserted-by":"publisher","first-page":"1201","DOI":"10.1111\/jam.13246","volume":"121","author":"SM Raszl","year":"2016","unstructured":"Raszl SM, Froelich BA, Vieira CR, Blackwood AD, Noble RT (2016) Vibrio parahaemolyticus and Vibrio vulnificus in South America: water, seafood and human infections. J Appl Microbiol 121:1201\u20131222","journal-title":"J Appl Microbiol"},{"key":"827_CR16","doi-asserted-by":"publisher","first-page":"1266","DOI":"10.1111\/j.1365-2958.2010.07172.x","volume":"76","author":"RJ Schulze","year":"2010","unstructured":"Schulze RJ, Chen S, Kumru OS, Z\u00fcckert WR (2010) Translocation of Borrelia burgdorferi surface lipoprotein OspA through the outer membrane requires an unfolded conformation and can initiate at the C-terminus. Mol Microbiol 76:1266\u20131278","journal-title":"Mol Microbiol"},{"key":"827_CR17","doi-asserted-by":"publisher","first-page":"784","DOI":"10.1038\/nbt1183-784","volume":"1","author":"R Simon","year":"1983","unstructured":"Simon R, PrieferU PuhlerA (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Nat Biotechnol 1:784\u2013791","journal-title":"Nat Biotechnol"},{"key":"827_CR18","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1128\/IAI.58.6.1769-1773.1990","volume":"58","author":"AC Wright","year":"1990","unstructured":"Wright AC, Simpson LM, Oliver JD, Morris JG (1990) Phenotypic evaluation of a capsular transposon mutants of Vibrio vulnificus. Infect Immun 58:1769\u20131773","journal-title":"Infect Immun"},{"key":"827_CR19","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1038\/35008635","volume":"2","author":"T Yakushi","year":"2000","unstructured":"Yakushi T, Masuda K, Narita S, Matsuyama S, Tokuda H (2000) A new ABC transporter mediating the detachment of lipid-modified proteins from membranes. Nat Cell Biol 2:212\u2013218","journal-title":"Nat Cell Biol"}],"container-title":["Antonie van Leeuwenhoek"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10482-016-0827-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10482-016-0827-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10482-016-0827-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T12:03:09Z","timestamp":1601294589000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10482-016-0827-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,2]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["827"],"URL":"https:\/\/doi.org\/10.1007\/s10482-016-0827-x","relation":{},"ISSN":["0003-6072","1572-9699"],"issn-type":[{"value":"0003-6072","type":"print"},{"value":"1572-9699","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,2]]}}}