{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T03:49:14Z","timestamp":1781322554524,"version":"3.54.1"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2019,11,28]],"date-time":"2019-11-28T00:00:00Z","timestamp":1574899200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2019,11,28]],"date-time":"2019-11-28T00:00:00Z","timestamp":1574899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000777","name":"University of Leeds","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000777","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/N007980\/1"],"award-info":[{"award-number":["BB\/N007980\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Antonie van Leeuwenhoek"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cloning natural product biosynthetic gene clusters from cultured or uncultured sources and their subsequent expression by genetically tractable heterologous hosts is an essential strategy for the elucidation and characterisation of novel microbial natural products. The availability of suitable expression hosts is a critical aspect of this workflow. In this work, we mutagenised five endogenous biosynthetic gene clusters from\n                    <jats:italic>Streptomyces albus<\/jats:italic>\n                    S4, which reduced the complexity of chemical extracts generated from the strain and eliminated antifungal and antibacterial bioactivity. We showed that the resulting quintuple mutant can express foreign biosynthetic gene clusters by heterologously producing actinorhodin, cinnamycin and prunustatin. We envisage that our strain will be a useful addition to the growing suite of heterologous expression hosts available for exploring microbial secondary metabolism.\n                  <\/jats:p>","DOI":"10.1007\/s10482-019-01360-x","type":"journal-article","created":{"date-parts":[[2019,11,28]],"date-time":"2019-11-28T14:02:42Z","timestamp":1574949762000},"page":"511-520","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["A chromatogram-simplified Streptomyces albus host for heterologous production of natural products"],"prefix":"10.1007","volume":"113","author":[{"given":"Asif","family":"Fazal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divya","family":"Thankachan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellie","family":"Harris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6156-8498","authenticated-orcid":false,"given":"Ryan F.","family":"Seipke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,11,28]]},"reference":[{"key":"1360_CR1","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1186\/1741-7007-8-109","volume":"8","author":"J Barke","year":"2010","unstructured":"Barke J, Seipke RF, Gr\u00fcschow S, Heavens D, Drou N, Bibb MJ, Goss RJM, Yu DW, Hutchings MI (2010) A mixed community of actinomycetes produce multiple antibiotics for the fungus farming ant Acromyrmex octospinosus. BMC Biol 8:109. https:\/\/doi.org\/10.1186\/1741-7007-8-109","journal-title":"BMC Biol"},{"key":"1360_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1086\/595011","volume":"48","author":"HW Boucher","year":"2009","unstructured":"Boucher HW, Talbot GH, Bradley JS, Edwards JE, Gilbert D, Rice LB, Scheld M, Spellberg B, Bartlett J (2009) Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clin Infecti Dis 48:1\u201312. https:\/\/doi.org\/10.1086\/595011","journal-title":"Clin Infecti Dis"},{"key":"1360_CR3","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1021\/sb500351f","volume":"4","author":"RE Cobb","year":"2015","unstructured":"Cobb RE, Wang Y, Zhao H (2015) High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR\/Cas system. ACS Synth Biol 4:723\u2013728. https:\/\/doi.org\/10.1021\/sb500351f","journal-title":"ACS Synth Biol"},{"key":"1360_CR4","doi-asserted-by":"publisher","first-page":"963","DOI":"10.1038\/nchembio.1659","volume":"10","author":"JR Doroghazi","year":"2014","unstructured":"Doroghazi JR, Albright JC, Goering AW, Haines RR, Tchalukov KA, Labeda DP, Metcalf WW (2014) A roadmap for natural product discovery based on large-scale genomics and metabolomics. Nat Chem Biol 10:963\u2013968. https:\/\/doi.org\/10.1038\/nchembio.1659","journal-title":"Nat Chem Biol"},{"key":"1360_CR5","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1111\/j.1751-7915.2010.00219.x","volume":"4","author":"JP Gomez-Escribano","year":"2011","unstructured":"Gomez-Escribano JP, Bibb MJ (2011) Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters. Microbial Biotechnol 4:207\u2013215. https:\/\/doi.org\/10.1111\/j.1751-7915.2010.00219.x","journal-title":"Microbial Biotechnol"},{"key":"1360_CR6","doi-asserted-by":"publisher","first-page":"434","DOI":"10.7164\/antibiotics.47.434","volume":"47","author":"H G\u00fcrtler","year":"1994","unstructured":"G\u00fcrtler H, Pedersen R, Anthoni U, Christophersen C, Nielsen PH, Wellington EM, Pedersen C, Bock K (1994) Albaflavenone, a sesquiterpene ketone with a zizaene skeleton produced by a streptomycete with a new rope morphology. J Antibiot 47:434\u2013439","journal-title":"J Antibiot"},{"key":"1360_CR7","doi-asserted-by":"publisher","first-page":"1541","DOI":"10.1073\/pnas.0337542100","volume":"100","author":"B Gust","year":"2003","unstructured":"Gust B, Challis GL, Fowler K, Kieser T, Chater KF (2003) PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc Natl Acad Sci USA 100:1541\u20131546. https:\/\/doi.org\/10.1073\/pnas.0337542100","journal-title":"Proc Natl Acad Sci USA"},{"key":"1360_CR8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41564-018-0110-1","volume":"3","author":"BM Hover","year":"2018","unstructured":"Hover BM, Kim S-H, Katz M, Charlop-Powers Z, Owen JG, Ternei MA, Maniko J, Estrela AB, Molina H, Park S, Perlin DS, Brady SF (2018) Culture-independent discovery of the malacidins as calcium-dependent antibiotics with activity against multidrug-resistant Gram-positive pathogens. Nat Microbiol 3:1\u201310. https:\/\/doi.org\/10.1038\/s41564-018-0110-1","journal-title":"Nat Microbiol"},{"key":"1360_CR9","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1039\/C8NP00091C","volume":"4","author":"L Huo","year":"2019","unstructured":"Huo L, Hug JJ, Fu C, Bian X, Zhang Y, M\u00fcller R (2019) Heterologous expression of bacterial natural product biosynthetic pathways. Nat Prod Rep 4:206\u2013225. https:\/\/doi.org\/10.1039\/C8NP00091C","journal-title":"Nat Prod Rep"},{"key":"1360_CR10","doi-asserted-by":"publisher","first-page":"e69319","DOI":"10.1371\/journal.pone.0069319","volume":"8","author":"AC Jones","year":"2013","unstructured":"Jones AC, Gust B, Kulik A, Heide L, Buttner MJ, Bibb MJ (2013) Phage P1-Derived Artificial chromosomes facilitate heterologous expression of the FK506 gene cluster. PLoS ONE 8:e69319\u2013e69329. https:\/\/doi.org\/10.1371\/journal.pone.0069319","journal-title":"PLoS ONE"},{"key":"1360_CR11","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1099\/mic.0.000572","volume":"164","author":"R Joynt","year":"2018","unstructured":"Joynt R, Seipke RF (2018) A phylogenetic and evolutionary analysis of antimycin biosynthesis. Microbiology 164:28\u201339. https:\/\/doi.org\/10.1099\/mic.0.000572","journal-title":"Microbiology"},{"key":"1360_CR12","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1007\/s10295-015-1723-5","volume":"43","author":"L Katz","year":"2016","unstructured":"Katz L, Baltz RH (2016) Natural product discovery: past, present, and future. J Indian Microbiol Biotechnol 43:155\u2013176. https:\/\/doi.org\/10.1007\/s10295-015-1723-5","journal-title":"J Indian Microbiol Biotechnol"},{"key":"1360_CR13","volume-title":"Practical Streptomyces genetics","author":"TB Kieser","year":"2002","unstructured":"Kieser TB, Buttner MJ, Chater KF, Hopwood DA (2002) Practical Streptomyces genetics. The John Innes Foundation, Norwich"},{"key":"1360_CR14","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1038\/nchembio.1893","volume":"11","author":"E Kim","year":"2015","unstructured":"Kim E, Moore BS, Yoon YJ (2015) Reinvigorating natural product combinatorial biosynthesis with synthetic biology. Nat Chem Biol 11:649\u2013659. https:\/\/doi.org\/10.1038\/nchembio.1893","journal-title":"Nat Chem Biol"},{"key":"1360_CR15","doi-asserted-by":"publisher","first-page":"2646","DOI":"10.1073\/pnas.0914833107","volume":"107","author":"M Komatsu","year":"2010","unstructured":"Komatsu M, Uchiyama T, Omura S, Cane DE, Ikeda H (2010) Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism. Proc Natl Acad Sci USA 107:2646\u20132651. https:\/\/doi.org\/10.1073\/pnas.0914833107","journal-title":"Proc Natl Acad Sci USA"},{"key":"1360_CR16","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1021\/sb3001003","volume":"2","author":"M Komatsu","year":"2013","unstructured":"Komatsu M, Komatsu K, Koiwai H, Yamada Y, Kozone I, Izumikawa M, Hashimoto J, Takagi M, Omura S, Shin-ya K, Cane DE, Ikeda H (2013) Engineered Streptomyces avermitilis host for heterologous expression of biosynthetic gene cluster for secondary metabolites. ACS Synth Biol 2:384\u2013396. https:\/\/doi.org\/10.1021\/sb3001003","journal-title":"ACS Synth Biol"},{"key":"1360_CR17","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1021\/sb5003136","volume":"4","author":"J Liu","year":"2015","unstructured":"Liu J, Zhu X, Seipke RF, Zhang W (2015) Biosynthesis of antimycins with a reconstituted 3-formamidosalicylate pharmacophore in Escherichia coli. ACS Synth Biol 4:559\u2013565. https:\/\/doi.org\/10.1021\/sb5003136","journal-title":"ACS Synth Biol"},{"key":"1360_CR18","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1039\/C5NP00085H","volume":"33","author":"Y Luo","year":"2016","unstructured":"Luo Y, Enghiad B, Zhao H (2016) New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters. Nat Prod Rep 33:174\u2013182. https:\/\/doi.org\/10.1039\/C5NP00085H","journal-title":"Nat Prod Rep"},{"key":"1360_CR19","doi-asserted-by":"publisher","unstructured":"McLean TC, Hoskisson PA, Seipke RF (2016) Coordinate regulation of antimycin and candicidin biosynthesis. mSphere 1:e00305\u201316. https:\/\/doi.org\/10.1128\/mSphere.00305-16","DOI":"10.1128\/mSphere.00305-16"},{"key":"1360_CR20","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1016\/j.ymben.2018.09.004","volume":"49","author":"M Myronovskyi","year":"2018","unstructured":"Myronovskyi M, Rosenkr\u00e4nzer B, Nadmid S, Pujic P, Normand P, Luzhetskyy A (2018) Generation of a cluster-free Streptomyces albus chassis strains for improved heterologous expression of secondary metabolite clusters. Metab Eng 49:316\u2013324. https:\/\/doi.org\/10.1016\/j.ymben.2018.09.004","journal-title":"Metab Eng"},{"key":"1360_CR21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.biotechadv.2018.10.003","volume":"37","author":"KK Nepal","year":"2019","unstructured":"Nepal KK, Wang G (2019) Streptomycetes_ Surrogate hosts for the genetic manipulation of biosynthetic gene clusters and production of natural products. Biotechnol Adv 37:1\u201320. https:\/\/doi.org\/10.1016\/j.biotechadv.2018.10.003","journal-title":"Biotechnol Adv"},{"key":"1360_CR22","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1038\/nrd2201","volume":"6","author":"DJ Payne","year":"2006","unstructured":"Payne DJ, Gwynn MN, Holmes DJ, Pompliano DL (2006) Drugs for bad bugs: confronting the challenges of antibacterial discovery. Nat Rev Drug Discov 6:29\u201340. https:\/\/doi.org\/10.1038\/nrd2201","journal-title":"Nat Rev Drug Discov"},{"key":"1360_CR23","doi-asserted-by":"publisher","first-page":"3218","DOI":"10.1039\/c6sc04265a","volume":"8","author":"Z Qin","year":"2017","unstructured":"Qin Z, Munnoch JT, Devine R, Holmes NA, Seipke RF, Wilkinson KA, Wilkinson B, Hutchings MI (2017) Formicamycins, antibacterial polyketides produced by Streptomyces formicae isolated from African Tetraponera plant-ants. Chem Sci 8:3218\u20133227. https:\/\/doi.org\/10.1039\/c6sc04265a","journal-title":"Chem Sci"},{"key":"1360_CR24","doi-asserted-by":"publisher","unstructured":"Schniete JK, Cruz-Morales P, Selem Mojica N, Fern\u00e1ndez-Mart\u00ednez LT, Hunter IS, Barona-G\u00f3mez F, Hoskisson PA (2018) Expanding primary metabolism helps generate the metabolic robustness to facilitate antibiotic biosynthesis in Streptomyces. mBio 9:e02283\u201317\u201313. https:\/\/doi.org\/10.1128\/mBio.02283-17","DOI":"10.1128\/mBio.02283-17"},{"key":"1360_CR25","doi-asserted-by":"publisher","first-page":"e0116457","DOI":"10.1371\/journal.pone.0116457","volume":"10","author":"RF Seipke","year":"2015","unstructured":"Seipke RF (2015) Strain-level diversity of secondary metabolism in Streptomyces albus. PLoS ONE 10:e0116457\u2013e116514. https:\/\/doi.org\/10.1371\/journal.pone.0116457","journal-title":"PLoS ONE"},{"key":"1360_CR26","doi-asserted-by":"publisher","first-page":"e22028","DOI":"10.1371\/journal.pone.0022028","volume":"6","author":"RF Seipke","year":"2011","unstructured":"Seipke RF, Barke J, Brearley C, Hill L, Yu DW, Goss RJM, Hutchings MI (2011a) A Single Streptomyces symbiont makes multiple antifungals to support the fungus farming ant Acromyrmex octospinosus. PLoS ONE 6:e22028\u2013e22038. https:\/\/doi.org\/10.1371\/journal.pone.0022028","journal-title":"PLoS ONE"},{"key":"1360_CR27","doi-asserted-by":"publisher","first-page":"4270","DOI":"10.1128\/JB.05275-11","volume":"193","author":"RF Seipke","year":"2011","unstructured":"Seipke RF, Crossman L, Drou N, Heavens D, Bibb MJ, Caccamo M, Hutchings MI (2011b) Draft genome sequence of Streptomyces strain S4, a symbiont of the leaf-cutting ant Acromyrmex octospinosus. J Bacteriol 193:4270\u20134271. https:\/\/doi.org\/10.1128\/JB.05275-11","journal-title":"J Bacteriol"},{"key":"1360_CR28","doi-asserted-by":"publisher","first-page":"e253","DOI":"10.7717\/peerj.253","volume":"2","author":"RF Seipke","year":"2014","unstructured":"Seipke RF, Patrick E, Hutchings MI (2014) Regulation of antimycin biosynthesis by the orphan ECF RNA polymerase sigma factor \u03c3AntA. PeerJ 2:e253. https:\/\/doi.org\/10.7717\/peerj.253","journal-title":"PeerJ"},{"key":"1360_CR29","doi-asserted-by":"publisher","first-page":"1398","DOI":"10.1021\/acschembio.8b00298","volume":"13","author":"W Skyrud","year":"2018","unstructured":"Skyrud W, Liu J, Thankachan D, Cabrera M, Seipke RF, Zhang W (2018) Biosynthesis of the 15-membered ring depsipeptide neoantimycin. ACS Chem Biol 13:1398\u20131406. https:\/\/doi.org\/10.1021\/acschembio.8b00298","journal-title":"ACS Chem Biol"},{"key":"1360_CR30","doi-asserted-by":"publisher","first-page":"e64858","DOI":"10.1371\/journal.pone.0064858","volume":"8","author":"DC Stevens","year":"2013","unstructured":"Stevens DC, Conway KR, Pearce N, Villegas-Pe\u00f1aranda LR, Garza AG, Boddy CN (2013) Alternative sigma factor over-expression enables heterologous expression of a type II polyketide biosynthetic pathway in Escherichia coli. PLoS ONE 8:e64858. https:\/\/doi.org\/10.1371\/journal.pone.0064858","journal-title":"PLoS ONE"},{"key":"1360_CR31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12934-015-0335-0","volume":"14","author":"A Thanapipatsiri","year":"2015","unstructured":"Thanapipatsiri A, Claesen J, Gomez-Escribano JP, Bibb M, Thamchaipenet A (2015) A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes. Microb Cell Fact 14:1\u201311. https:\/\/doi.org\/10.1186\/s12934-015-0335-0","journal-title":"Microb Cell Fact"},{"key":"1360_CR32","doi-asserted-by":"publisher","first-page":"e02828","DOI":"10.1128\/AEM.02828-17","volume":"84","author":"NM Vior","year":"2018","unstructured":"Vior NM, Lacret R, Chandra G, Dorai-Raj S, Trick M, Truman AW (2018) Discovery and biosynthesis of the antibiotic bicyclomycin in distantly related bacterial classes. Appl Environ Microbiol 84:e02828\u2013e2917. https:\/\/doi.org\/10.1128\/AEM.02828-17","journal-title":"Appl Environ Microbiol"},{"key":"1360_CR33","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1038\/ja.2014.37","volume":"67","author":"X Wang","year":"2014","unstructured":"Wang X, Shaaban KA, Elshahawi SI, Ponomareva LV, Sunkara M, Copley GC, Hower JC, Morris AJ, Kharel MK, Thorson JS (2014) Mullinamides A and B, new cyclopeptides produced by the Ruth Mullins coal mine fire isolate Streptomyces sp. RM-27-46. J Antibiot 67:571\u2013575. https:\/\/doi.org\/10.1038\/ja.2014.37","journal-title":"J Antibiot"},{"key":"1360_CR34","doi-asserted-by":"publisher","first-page":"1402","DOI":"10.7164\/antibiotics.34.1402","volume":"34","author":"DJ Warnick-Pickle","year":"1981","unstructured":"Warnick-Pickle DJ, Byrne KM, Pandey RC, White RJ (1981) Fredericamycin A, a new antitumor antibiotic. II. Biological properties. J Antibiot 34:1402\u20131407","journal-title":"J Antibiot"},{"key":"1360_CR35","doi-asserted-by":"publisher","first-page":"W237","DOI":"10.1093\/nar\/gkv437","volume":"43","author":"T Weber","year":"2015","unstructured":"Weber T, Blin K, Duddela S, Krug D, Kim HU, Bruccoleri R, Lee SY, Fischbach MA, M\u00fcller R, Wohlleben W, Breitling R, Takano E, Medema MH (2015) antiSMASH 3.0\u2014a comprehensive resource for the genome mining of biosynthetic gene clusters. Nucleic Acids Res 43:W237\u2013W243. https:\/\/doi.org\/10.1093\/nar\/gkv437","journal-title":"Nucleic Acids Res"},{"key":"1360_CR36","doi-asserted-by":"publisher","first-page":"4316","DOI":"10.1073\/pnas.0230516100","volume":"100","author":"DA Widdick","year":"2003","unstructured":"Widdick DA, Dodd HM, Barraille P, White J, Stein TH, Chater KF, Gasson MJ, Bibb MJ (2003) Cloning and engineering of the cinnamycin biosynthetic gene cluster from Streptomyces cinnamoneus cinnamoneus DSM 40005. Proc Natl Acad Sci USA 100:4316\u20134321. https:\/\/doi.org\/10.1073\/pnas.0230516100","journal-title":"Proc Natl Acad Sci USA"},{"key":"1360_CR37","doi-asserted-by":"publisher","first-page":"1957","DOI":"10.1073\/pnas.1319584111","volume":"111","author":"K Yamanaka","year":"2014","unstructured":"Yamanaka K, Reynolds KA, Kersten RD, Ryan KS, Gonzalez DJ, Nizet V, Dorrestein PC, Moore BS (2014) Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A. Proc Natl Acad Sci USA 111:1957\u20131962. https:\/\/doi.org\/10.1073\/pnas.1319584111","journal-title":"Proc Natl Acad Sci USA"},{"key":"1360_CR38","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1039\/C6NP00017G","volume":"00","author":"MM Zhang","year":"2016","unstructured":"Zhang MM, Wang Y, Ang EL, Zhao H (2016) Engineering microbial hosts for production of bacterial natural products. Nat Prod Rep 00:1\u201325. https:\/\/doi.org\/10.1039\/C6NP00017G","journal-title":"Nat Prod Rep"}],"container-title":["Antonie van Leeuwenhoek"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10482-019-01360-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10482-019-01360-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10482-019-01360-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T19:26:33Z","timestamp":1606418793000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10482-019-01360-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,28]]},"references-count":38,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["1360"],"URL":"https:\/\/doi.org\/10.1007\/s10482-019-01360-x","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/612291","asserted-by":"object"}]},"ISSN":["0003-6072","1572-9699"],"issn-type":[{"value":"0003-6072","type":"print"},{"value":"1572-9699","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,28]]},"assertion":[{"value":"30 August 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 November 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 November 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that there are no conflicts of interest and that the funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}