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Metagenomic studies of the upper oxic layers of geothermal soils of the Favara Grande, Pantelleria, Italy, revealed the presence of various methane-oxidizing bacteria, and resulted in a near complete metagenome assembled genome (MAG) of an aerobic methanotroph, which was classified as a <jats:italic>Methylobacter<\/jats:italic> species. In this study, the <jats:italic>Methylobacter<\/jats:italic> sp. B2 MAG was used to investigate its metabolic potential and phylogenetic affiliation. The MAG has a size of 4,086,539\u00a0bp, consists of 134 contigs and 3955 genes were found, of which 3902 were protein coding genes. All genes for CH<jats:sub>4<\/jats:sub> oxidation to CO<jats:sub>2<\/jats:sub> were detected, including <jats:italic>pmoCAB<\/jats:italic> encoding particulate methane monooxygenase (pMMO) and <jats:italic>xoxF<\/jats:italic> encoding a methanol dehydrogenase. No gene encoding a formaldehyde dehydrogenase was present and the formaldehyde to formate conversion follows the tetrahydromethanopterin (H<jats:sub>4<\/jats:sub>MPT) pathway. \u201c<jats:italic>Ca.<\/jats:italic> Methylobacter favarea\u201d B2 uses the Ribulose-Mono-Phosphate (RuMP) pathway for carbon fixation. Analysis of the MAG indicates that Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> antiporters and the urease system might be important in the maintenance of pH homeostasis of this strain to cope with acidic conditions. So far, thermoacidophilic <jats:italic>Methylobacter<\/jats:italic> species have not been isolated, however this study indicates that members of the genus <jats:italic>Methylobacter<\/jats:italic> can be found in distinct ecosystems and their presence is not restricted to freshwater or marine sediments.<\/jats:p>","DOI":"10.1007\/s10482-021-01525-7","type":"journal-article","created":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T12:25:48Z","timestamp":1612959948000},"page":"313-324","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Draft genome of a novel methanotrophic Methylobacter sp. from the volcanic soils of Pantelleria Island"],"prefix":"10.1007","volume":"114","author":[{"given":"Carmen","family":"Hogendoorn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nunzia","family":"Picone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Femke","family":"van Hout","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Vijverberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7720-4883","authenticated-orcid":false,"given":"Lianna","family":"Poghosyan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theo A.","family":"van Alen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6279-2363","authenticated-orcid":false,"given":"Jeroen","family":"Frank","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arjan","family":"Pol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7657-8533","authenticated-orcid":false,"given":"Antonia L.","family":"Gagliano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4691-7039","authenticated-orcid":false,"given":"Mike S. 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