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In this study, we describe a newly isolated strain of <jats:italic>D. kuznetsovii<\/jats:italic>, strain TPOSR, and compare its metabolism to the type strain <jats:italic>D. kuznetsovii<\/jats:italic> 17<jats:sup>T<\/jats:sup>. Both strains grow on a large variety of alcohols, such as methanol, ethanol and propane-diols, coupled to the reduction of sulphate. Strain 17<jats:sup>T<\/jats:sup> metabolizes methanol via two routes, one involving a cobalt-dependent methyl transferase and the other using a cobalt-independent alcohol dehydrogenase. However, strain TPOSR, which shares 97% average nucleotide identity with <jats:italic>D. kuznetsovii<\/jats:italic> strain 17<jats:sup>T<\/jats:sup>, lacks several genes from the methyl transferase operon found in strain 17<jats:sup>T<\/jats:sup>. The gene encoding the catalytically active methyl transferase subunit B is missing, indicating that strain TPOSR utilizes the alcohol dehydrogenase pathway exclusively. Both strains grew with methanol during cobalt starvation, but growth was impaired. Strain 17<jats:sup>T<\/jats:sup> was more sensitive to cobalt deficiency, due to the repression of its methyl transferase system. Our findings shed light on the metabolic diversity of <jats:italic>D. kuznetsovii<\/jats:italic> and their metabolic differences of encoding one or two routes for the conversion of methanol.<\/jats:p>","DOI":"10.1007\/s10482-024-01937-1","type":"journal-article","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T15:04:08Z","timestamp":1709305448000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Alcohol dehydrogenase system acts as the sole pathway for methanol oxidation in Desulfofundulus kuznetsovii strain TPOSR"],"prefix":"10.1007","volume":"117","author":[{"given":"Lukas","family":"Friedeheim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sjef","family":"Boeren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"S\u00e1nchez-Andrea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfons J. 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