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The corrinoid-dependent methyltransferases are present in all domains of life and assumed to be exclusive for methyl-groups. In <jats:italic>Methanosarcina<\/jats:italic>, however, trace ethane production from ethanol has been shown in vivo<jats:italic>,<\/jats:italic> which led to the hypothesis that corrinoid-dependent methanol-specific methyltransferases are promiscuous towards also accepting ethyl-groups. Here, we show that the conversion of ethanol to trace amounts of ethane in <jats:italic>Methanosarcina acetivorans<\/jats:italic> involves homologous reactions of the known methanol-to-methane metabolism. The methanol methyltransferase (MtaB) activates ethanol and loads the ethyl-group onto the corrinoid-containing methyl-accepting protein (MtaC). Besides MtaCB, substrate promiscuity in corrinoid:coenzyme M methyltransferase (MtaA) and methyl-coenzyme M reductase (Mcr) are required to grant the microbe the capacity for ethane production. We show that the MtaCB subunits of <jats:italic>M. acetivorans<\/jats:italic> can activate ethanol, however, the ethane yields compared to methane are ca. 3 orders of magnitude lower. The ethyl-transfer capability was confirmed for each of the three MtaCB isozyme by quantifying the amount of ethane produced by <jats:italic>mtaCB<\/jats:italic> double deletion strains during growth in ethanol-supplemented media and in resting-cell suspensions. Ethane formation requires the cells to be grown on methanol to trigger the expression of the <jats:italic>mtaCB<\/jats:italic> genes, and detectable ethane formation starts only after all methanol has been consumed. Demonstrating that corrinoid-dependent methanol-specific methyltransferases process ethyl groups extends the pool of reactions to be considered in metabolic networks and suggests possible routes for biogenic ethane in nature.<\/jats:p>","DOI":"10.1007\/s10482-025-02165-x","type":"journal-article","created":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T13:44:53Z","timestamp":1758375893000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Methanosarcina acetivorans requires methanol:coenzyme M methyltransferases for ethane formation from ethanol"],"prefix":"10.1007","volume":"118","author":[{"given":"Tejas","family":"Somvanshi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai Anh","family":"Tran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jichen","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvan","family":"Scheller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,9,20]]},"reference":[{"key":"2165_CR1","doi-asserted-by":"publisher","first-page":"2251","DOI":"10.1111\/febs.17409","volume":"292","author":"J Bao","year":"2025","unstructured":"Bao J, Somvanshi T, Tian Y et al (2025) Nature AND nurture: enabling formate-dependent growth in Methanosarcina acetivorans. 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