{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T09:04:04Z","timestamp":1775552644873,"version":"3.50.1"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Antonie van Leeuwenhoek"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1007\/s10482-026-02280-3","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T23:02:04Z","timestamp":1773874924000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative genomic assessment of probiotic potential across the Anoxybacillaceae family"],"prefix":"10.1007","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0347-3412","authenticated-orcid":false,"given":"Mohamed Amine","family":"Gomri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5650-9737","authenticated-orcid":false,"given":"Hassina","family":"Bendjaballah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6854-1925","authenticated-orcid":false,"given":"Mohamed","family":"El Hadef El Okki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"issue":"4","key":"2280_CR2","doi-asserted-by":"publisher","first-page":"e1006946","DOI":"10.1371\/journal.pcbi.1006946","volume":"15","author":"H Akarsu","year":"2019","unstructured":"Akarsu H, Bordes P, Mansour M, Bigot D-J, Genevaux P, Falquet L (2019) TASmania: a bacterial toxin-antitoxin systems database. PLoS Comput Biol 15(4):e1006946. https:\/\/doi.org\/10.1371\/journal.pcbi.1006946","journal-title":"PLoS Comput Biol"},{"issue":"D1","key":"2280_CR3","doi-asserted-by":"publisher","first-page":"D690","DOI":"10.1093\/nar\/gkac920","volume":"51","author":"BP Alcock","year":"2022","unstructured":"Alcock BP, Huynh W, Chalil R, Smith KW, Raphenya AR, Wlodarski MA, Edalatmand A, Petkau A, Syed SA, Tsang KK, Baker SJC, Dave M, McCarthy MC, Mukiri KM, Nasir JA, Golbon B, Imtiaz H, Jiang X, Kaur K, Kwong M, Liang ZC, Niu KC, Shan P, Yang JYJ, Gray KL et al (2022) CARD 2023: expanded curation, support for machine learning, and resistome prediction at the Comprehensive Antibiotic Resistance Database. Nucleic Acids Res 51(D1):D690\u2013D699. https:\/\/doi.org\/10.1093\/nar\/gkac920","journal-title":"Nucleic Acids Res"},{"issue":"7","key":"2280_CR4","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1038\/nbt.4163","volume":"36","author":"AP Arkin","year":"2018","unstructured":"Arkin AP, Cottingham RW, Henry CS, Harris NL, Stevens RL, Maslov S, Dehal P, Ware D, Perez F, Canon S, Sneddon MW, Henderson ML, Riehl WJ, Murphy-Olson D, Chan SY, Kamimura RT, Kumari S, Drake MM, Brettin TS, Glass EM, Chivian D, Gunter D, Weston DJ, Allen BH, Baumohl J et al (2018) KBase: the United States department of energy systems biology knowledgebase. Nat Biotechnol 36(7):566\u2013569. https:\/\/doi.org\/10.1038\/nbt.4163","journal-title":"Nat Biotechnol"},{"issue":"W1","key":"2280_CR5","doi-asserted-by":"publisher","first-page":"W29","DOI":"10.1093\/nar\/gkab335","volume":"49","author":"K Blin","year":"2021","unstructured":"Blin K, Shaw S, Kloosterman AM, Charlop-Powers Z, van Wezel GP, Medema MH, Weber T (2021) antiSMASH 6.0: Improving cluster detection and comparison capabilities. Nucleic Acids Res 49(W1):W29\u2013W35. https:\/\/doi.org\/10.1093\/nar\/gkab335","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"2280_CR6","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1038\/s41592-021-01101-x","volume":"18","author":"B Buchfink","year":"2021","unstructured":"Buchfink B, Reuter K, Drost H-G (2021) Sensitive protein alignments at tree-of-life scale using DIAMOND. Nat Methods 18(4):366\u2013368. https:\/\/doi.org\/10.1038\/s41592-021-01101-x","journal-title":"Nat Methods"},{"issue":"12","key":"2280_CR7","doi-asserted-by":"publisher","first-page":"5825","DOI":"10.1093\/molbev\/msab293","volume":"38","author":"CP Cantalapiedra","year":"2021","unstructured":"Cantalapiedra CP, Hern\u00e1ndez-Plaza A, Letunic I, Bork P, Huerta-Cepas J (2021) eggNOG-mapper v2: functional annotation, orthology assignments, and domain prediction at the metagenomic scale. Mol Biol Evol 38(12):5825\u20135829. https:\/\/doi.org\/10.1093\/molbev\/msab293","journal-title":"Mol Biol Evol"},{"issue":"1","key":"2280_CR8","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1111\/jam.15199","volume":"132","author":"FM Carpi","year":"2022","unstructured":"Carpi FM, Coman MM, Silvi S, Picciolini M, Verdenelli MC, Napolioni V (2022) Comprehensive pan-genome analysis of Lactiplantibacillus plantarum complete genomes. J Appl Microbiol 132(1):592\u2013604. https:\/\/doi.org\/10.1111\/jam.15199","journal-title":"J Appl Microbiol"},{"issue":"2","key":"2280_CR9","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1093\/bib\/bbw108","volume":"19","author":"J Chen","year":"2016","unstructured":"Chen J, Guo M, Wang X, Liu B (2016) A comprehensive review and comparison of different computational methods for protein remote homology detection. Brief Bioinform 19(2):231\u2013244. https:\/\/doi.org\/10.1093\/bib\/bbw108","journal-title":"Brief Bioinform"},{"issue":"D1","key":"2280_CR10","doi-asserted-by":"publisher","first-page":"D694","DOI":"10.1093\/nar\/gkv1239","volume":"44","author":"L Chen","year":"2015","unstructured":"Chen L, Zheng D, Liu B, Yang J, Jin Q (2015) VFDB 2016: hierarchical and refined dataset for big data analysis\u201410 years on. Nucleic Acids Res 44(D1):D694\u2013D697. https:\/\/doi.org\/10.1093\/nar\/gkv1239","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"2280_CR11","doi-asserted-by":"publisher","first-page":"101","DOI":"10.3390\/applmicrobiol5040101","volume":"5","author":"AZ Ghailan","year":"2025","unstructured":"Ghailan AZ, Niamah AK (2025) Streptococcus thermophilus: metabolic properties, functional features, and useful applications. Appl Microbiol 5(4):101. https:\/\/doi.org\/10.3390\/applmicrobiol5040101","journal-title":"Appl Microbiol"},{"issue":"9","key":"2280_CR12","doi-asserted-by":"publisher","first-page":"1236","DOI":"10.1093\/bioinformatics\/btu031","volume":"30","author":"P Jones","year":"2014","unstructured":"Jones P, Binns D, Chang H-Y, Fraser M, Li W, McAnulla C, McWilliam H, Maslen J, Mitchell A, Nuka G, Pesseat S, Quinn AF, Sangrador-Vegas A, Scheremetjew M, Yong S-Y, Lopez R, Hunter S (2014) InterProScan 5: genome-scale protein function classification. Bioinformatics 30(9):1236\u20131240. https:\/\/doi.org\/10.1093\/bioinformatics\/btu031","journal-title":"Bioinformatics"},{"issue":"2","key":"2280_CR13","doi-asserted-by":"publisher","first-page":"347","DOI":"10.3390\/microorganisms12020347","volume":"12","author":"N Kapse","year":"2024","unstructured":"Kapse N, Pisu V, Dhakephalkar T, Margale P, Shetty D, Wagh S, Dagar S, Dhakephalkar PK (2024) Unveiling the probiotic potential of Streptococcus thermophilus MCC0200: insights from in vitro studies corroborated with genome analysis. Microorganisms 12(2):347. https:\/\/doi.org\/10.3390\/microorganisms12020347","journal-title":"Microorganisms"},{"key":"2280_CR14","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1007\/978-981-13-0329-6_5","volume-title":"Extremophiles in Eurasian ecosystems: ecology, diversity, and applications","author":"A Margaryan","year":"2018","unstructured":"Margaryan A, Shahinyan G, Hovhannisyan P, Panosyan H, Birkeland N-K, Trchounian A (2018) Geobacillus and Anoxybacillus spp. from terrestrial geothermal springs worldwide: diversity and biotechnological applications. In: Egamberdieva D, Birkeland N-K, Panosyan H, Li W-J (eds) Extremophiles in Eurasian ecosystems: ecology, diversity, and applications. Springer Singapore, Singapore, pp 119\u2013166. https:\/\/doi.org\/10.1007\/978-981-13-0329-6_5"},{"key":"2280_CR15","doi-asserted-by":"publisher","first-page":"1302480","DOI":"10.3389\/fmicb.2023.1302480","volume":"14","author":"S Mazhar","year":"2024","unstructured":"Mazhar S, Simon A, Khokhlova E, Colom J, Leeuwendaal N, Deaton J, Rea K (2024) In vitro safety and functional characterization of the novel Bacillus coagulans strain CGI314. Front Microbiol 14:1302480. https:\/\/doi.org\/10.3389\/fmicb.2023.1302480","journal-title":"Front Microbiol"},{"issue":"1","key":"2280_CR16","doi-asserted-by":"publisher","first-page":"33748","DOI":"10.1038\/s41598-025-98392-3","volume":"15","author":"IN Najar","year":"2025","unstructured":"Najar IN, Sharma P, Das R, Mondal K, Singh AK, Radha A, Sharma V, Sharma S, Thakur N, Gandhi SG, Kumar V (2025) Unveiling the probiotic potential of the genus Geobacillus through comparative genomics and in silico analysis. Sci Rep 15(1):33748. https:\/\/doi.org\/10.1038\/s41598-025-98392-3","journal-title":"Sci Rep"},{"issue":"7","key":"2280_CR17","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1101\/gr.186072.114","volume":"25","author":"DH Parks","year":"2015","unstructured":"Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW (2015) CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 25(7):1043\u20131055. https:\/\/doi.org\/10.1101\/gr.186072.114","journal-title":"Genome Res"},{"key":"2280_CR18","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006528","author":"I Patel","year":"2024","unstructured":"Patel I, Bello S, Gupta RS (2024) Phylogenomic and molecular marker based studies to clarify the evolutionary relationships amongst Anoxybacillus species and demarcation of the family Anoxybacillaceae and some of its constituent genera. Int J Syst Evol Microbiol. https:\/\/doi.org\/10.1099\/ijsem.0.006528","journal-title":"Int J Syst Evol Microbiol"},{"key":"2280_CR19","doi-asserted-by":"publisher","unstructured":"P\u00e9rez-Ju\u00e1rez CM, Flores-Gallegos AC, Cruz-Requena M, S\u00e1enz-Galindo A, Cobos-Puc L, Rodr\u00edguez-Herrera R (2022) Chapter 11 Functionality of thermophilic bacteria as probiotics. In: Kuddus M (ed) Microbial Extremozymes. Academic Press, pp 147\u2013160 https:\/\/doi.org\/10.1016\/B978-0-12-822945-3.00008-7","DOI":"10.1016\/B978-0-12-822945-3.00008-7"},{"issue":"3","key":"2280_CR20","doi-asserted-by":"publisher","first-page":"858","DOI":"10.12944\/CRNFSJ.10.3.4","volume":"10","author":"A Poshadri","year":"2022","unstructured":"Poshadri A, Hw D, Um K, Sd K (2022) Bacillus coagulans and its spore as potential probiotics in the production of novel shelf-stable foods. Current Res Nutr Food Sci J 10(3):858\u2013870. https:\/\/doi.org\/10.12944\/CRNFSJ.10.3.4","journal-title":"Current Res Nutr Food Sci J"},{"issue":"8","key":"2280_CR21","doi-asserted-by":"publisher","first-page":"e000206","DOI":"10.1099\/mgen.0.000206","volume":"4","author":"J Robertson","year":"2018","unstructured":"Robertson J, Nash JHE (2018) MOB-suite: software tools for clustering, reconstruction and typing of plasmids from draft assemblies. Microb Genomics 4(8):e000206. https:\/\/doi.org\/10.1099\/mgen.0.000206","journal-title":"Microb Genomics"},{"key":"2280_CR22","doi-asserted-by":"publisher","DOI":"10.1080\/17460913.2025.2492472","author":"YNV Sabino","year":"2025","unstructured":"Sabino YNV, Paiva AD, Fonseca BR, Medeiros JD, Machado ABF (2025) Deciphering probiotic potential: A comprehensive guide to probiogenomic analyses. Future Microbiol. https:\/\/doi.org\/10.1080\/17460913.2025.2492472","journal-title":"Future Microbiol"},{"issue":"16","key":"2280_CR23","doi-asserted-by":"publisher","first-page":"2775","DOI":"10.3390\/foods14162775","volume":"14","author":"M Salvador","year":"2025","unstructured":"Salvador M, Cond\u00f3n S, Gay\u00e1n E (2025) Parageobacillus and Geobacillus spp.: From food spoilage to beneficial food applications. Foods 14(16):2775. https:\/\/doi.org\/10.3390\/foods14162775","journal-title":"Foods"},{"key":"2280_CR100","unstructured":"Seemann T (2017) Abricate 1.2. GitHub. https:\/\/github.com\/tseemann\/abricate"},{"issue":"1","key":"2280_CR24","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1186\/s13568-025-01941-2","volume":"15","author":"R Srivastava","year":"2025","unstructured":"Srivastava R, Chamadia B, Behare PV, Chauhan K, Kumar S, Mondal G, Tyagi N (2025) Characterization and probiotic potential of thermotolerant lactic acid bacteria from silage for ensiling crop residues. AMB Express 15(1):140. https:\/\/doi.org\/10.1186\/s13568-025-01941-2","journal-title":"AMB Express"},{"issue":"10","key":"2280_CR25","doi-asserted-by":"publisher","first-page":"1955","DOI":"10.3390\/microorganisms12101955","volume":"12","author":"M Tsifintaris","year":"2024","unstructured":"Tsifintaris M, Kiousi DE, Repanas P, Kamarinou CS, Kavakiotis I, Galanis A (2024) Probio-Ichnos: A database of microorganisms with in vitro probiotic properties. Microorganisms 12(10):1955","journal-title":"Microorganisms"},{"key":"2280_CR26","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1146\/annurev-food-061120-014739","volume":"12","author":"Y Xiao","year":"2021","unstructured":"Xiao Y, Zhai Q, Zhang H, Chen W, Hill C (2021) Gut colonization mechanisms of Lactobacillus and Bifidobacterium: an argument for personalized designs. Annu Rev Food Sci Technol 12:213\u2013233. https:\/\/doi.org\/10.1146\/annurev-food-061120-014739. ((Volume 12, 2021))","journal-title":"Annu Rev Food Sci Technol"},{"issue":"21","key":"2280_CR27","doi-asserted-by":"publisher","first-page":"3340","DOI":"10.1093\/bioinformatics\/btx433","volume":"33","author":"Z Xie","year":"2017","unstructured":"Xie Z, Tang H (2017) ISEScan: automated identification of insertion sequence elements in prokaryotic genomes. Bioinformatics 33(21):3340\u20133347. https:\/\/doi.org\/10.1093\/bioinformatics\/btx433","journal-title":"Bioinformatics"},{"issue":"W1","key":"2280_CR28","doi-asserted-by":"publisher","first-page":"W95","DOI":"10.1093\/nar\/gky418","volume":"46","author":"H Zhang","year":"2018","unstructured":"Zhang H, Yohe T, Huang L, Entwistle S, Wu P, Yang Z, Busk PK, Xu Y, Yin Y (2018) dbCAN2: a meta server for automated carbohydrate-active enzyme annotation. Nucleic Acids Res 46(W1):W95\u2013W101. https:\/\/doi.org\/10.1093\/nar\/gky418","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"2280_CR29","doi-asserted-by":"publisher","first-page":"313","DOI":"10.3390\/fermentation11060313","volume":"11","author":"D Zieli\u0144ska","year":"2025","unstructured":"Zieli\u0144ska D, Krawczyk M, Neffe-Skoci\u0144ska K (2025) Thermotolerant probiotic\u2014the potential of improving the survivability of beneficial bacteria. Fermentation 11(6):313. https:\/\/doi.org\/10.3390\/fermentation11060313","journal-title":"Fermentation"}],"container-title":["Antonie van Leeuwenhoek"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10482-026-02280-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10482-026-02280-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10482-026-02280-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T08:22:21Z","timestamp":1775550141000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10482-026-02280-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,18]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["2280"],"URL":"https:\/\/doi.org\/10.1007\/s10482-026-02280-3","relation":{},"ISSN":["0003-6072","1572-9699"],"issn-type":[{"value":"0003-6072","type":"print"},{"value":"1572-9699","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,18]]},"assertion":[{"value":"24 December 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 March 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 March 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}],"article-number":"69"}}