{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T14:45:55Z","timestamp":1785768355854,"version":"3.56.0"},"reference-count":73,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2021YFA0910800"],"award-info":[{"award-number":["2021YFA0910800"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["41876188"],"award-info":[{"award-number":["41876188"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Antonie van Leeuwenhoek"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1007\/s10482-026-02370-2","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T13:09:52Z","timestamp":1784207392000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated nutrient removal and biodiesel potential of newly isolated Desmodesmus sp. F1 and Scenedesmus sp. Fs from synthetic municipal wastewater"],"prefix":"10.1007","volume":"119","author":[{"given":"Chao","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youping","family":"Nan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangxin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"2370_CR1","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.jbiosc.2013.12.021","volume":"118","author":"MA Alam","year":"2014","unstructured":"Alam MA, Wan C, Guo SL et al (2014) Characterization of the flocculating agent from the spontaneously flocculating microalga Chlorella vulgaris JSC-7. J Biosci Bioeng 118:29\u201333. https:\/\/doi.org\/10.1016\/j.jbiosc.2013.12.021","journal-title":"J Biosci Bioeng"},{"key":"2370_CR2","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.biortech.2015.01.018","volume":"181","author":"PD \u00c1lvarez-D\u00edaz","year":"2015","unstructured":"\u00c1lvarez-D\u00edaz PD, Ruiz J, Arbib Z et al (2015) Wastewater treatment and biodiesel production by Scenedesmus obliquus in a two-stage cultivation process. Bioresour Technol 181:90\u201396. https:\/\/doi.org\/10.1016\/j.biortech.2015.01.018","journal-title":"Bioresour Technol"},{"key":"2370_CR3","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1139\/o59-099","volume":"37","author":"EG Bligh","year":"1959","unstructured":"Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911\u2013917. https:\/\/doi.org\/10.1139\/o59-099","journal-title":"Can J Biochem Physiol"},{"key":"2370_CR4","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1016\/j.rser.2012.11.030","volume":"19","author":"T Cai","year":"2013","unstructured":"Cai T, Park SY, Li Y (2013) Nutrient recovery from wastewater streams by microalgae: status and prospects. Renew Sust Energ Rev 19:360\u2013369. https:\/\/doi.org\/10.1016\/j.rser.2012.11.030","journal-title":"Renew Sust Energ Rev"},{"key":"2370_CR5","doi-asserted-by":"publisher","first-page":"150296","DOI":"10.1016\/j.scitotenv.2021.150296","volume":"805","author":"F Castrogiovanni","year":"2022","unstructured":"Castrogiovanni F, Borea L, Corpuz MVA et al (2022) Innovative encapsulated self-forming dynamic bio-membrane bioreactor (ESFDMBR) for efficient wastewater treatment and fouling control. Sci Total Environ 805:150296. https:\/\/doi.org\/10.1016\/j.scitotenv.2021.150296","journal-title":"Sci Total Environ"},{"key":"2370_CR6","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1007\/s11157-024-09691-6","volume":"23","author":"AI Cengiz","year":"2024","unstructured":"Cengiz AI, Guven H, Ozgun H et al (2024) Enhanced energy recovery in municipal wastewater treatment plants through co-digestion by anaerobic membrane bioreactors: current status and future perspectives. Rev Environ Sci Bio\/technol 23:385\u2013410. https:\/\/doi.org\/10.1007\/s11157-024-09691-6","journal-title":"Rev Environ Sci Bio\/technol"},{"key":"2370_CR7","doi-asserted-by":"publisher","first-page":"20399","DOI":"10.1007\/s11356-017-9575-3","volume":"25","author":"R Chaudhary","year":"2018","unstructured":"Chaudhary R, Dikshit AK, Tong YW (2018) Carbon-dioxide biofixation and phycoremediation of municipal wastewater using Chlorella vulgaris and Scenedesmus obliquus. Environ Sci Pollut Res Int 25:20399\u201320406. https:\/\/doi.org\/10.1007\/s11356-017-9575-3","journal-title":"Environ Sci Pollut Res Int"},{"key":"2370_CR8","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1016\/j.apenergy.2014.10.032","volume":"137","author":"G Chen","year":"2015","unstructured":"Chen G, Zhao L, Qi Y (2015) Enhancing the productivity of microalgae cultivated in wastewater toward biofuel production: a critical review. Appl Energy 137:282\u2013291. https:\/\/doi.org\/10.1016\/j.apenergy.2014.10.032","journal-title":"Appl Energy"},{"key":"2370_CR9","doi-asserted-by":"publisher","first-page":"827513","DOI":"10.3389\/fbioe.2022.827513","volume":"10","author":"Z Chen","year":"2022","unstructured":"Chen Z, Chen Y, Zhang H et al (2022) Evaluation of Euglena gracilis 815 as a new candidate for biodiesel production. Front Bioeng Biotechnol 10:827513. https:\/\/doi.org\/10.3389\/fbioe.2022.827513","journal-title":"Front Bioeng Biotechnol"},{"key":"2370_CR10","doi-asserted-by":"publisher","first-page":"137473","DOI":"10.1016\/j.chemosphere.2022.137473","volume":"313","author":"J Chen","year":"2023","unstructured":"Chen J, Ren Z, Li Z et al (2023) Interaction of Scenedesmus quadricauda and native bacteria in marine biopharmaceutical wastewater for desirable lipid production and wastewater treatment. Chemosphere 313:137473. https:\/\/doi.org\/10.1016\/j.chemosphere.2022.137473","journal-title":"Chemosphere"},{"key":"2370_CR11","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/j.biortech.2012.12.176","volume":"131","author":"S Cho","year":"2013","unstructured":"Cho S, Lee N, Park S et al (2013) Microalgae cultivation for bioenergy production using wastewaters from a municipal WWTP as nutritional sources. Bioresour Technol 131:515\u2013520. https:\/\/doi.org\/10.1016\/j.biortech.2012.12.176","journal-title":"Bioresour Technol"},{"key":"2370_CR12","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.marpolbul.2014.01.006","volume":"80","author":"Y Collos","year":"2014","unstructured":"Collos Y, Harrison PJ (2014) Acclimation and toxicity of high ammonium concentrations to unicellular algae. Mar Pollut Bull 80:8\u201323. https:\/\/doi.org\/10.1016\/j.marpolbul.2014.01.006","journal-title":"Mar Pollut Bull"},{"key":"2370_CR13","doi-asserted-by":"publisher","first-page":"147475","DOI":"10.1016\/j.scitotenv.2021.147475","volume":"786","author":"MVA Corpuz","year":"2021","unstructured":"Corpuz MVA, Borea L, Senatore V et al (2021) Wastewater treatment and fouling control in an electro algae-activated sludge membrane bioreactor. Sci Total Environ 786:147475. https:\/\/doi.org\/10.1016\/j.scitotenv.2021.147475","journal-title":"Sci Total Environ"},{"key":"2370_CR14","doi-asserted-by":"publisher","first-page":"158337","DOI":"10.1016\/j.scitotenv.2022.158337","volume":"851","author":"EG de Morais","year":"2022","unstructured":"de Morais EG, Murillo AM, Lens PNL et al (2022) Selenium recovery from wastewater by the green microalgae Chlorella vulgaris and Scenedesmus sp. Sci Total Environ 851:158337. https:\/\/doi.org\/10.1016\/j.scitotenv.2022.158337","journal-title":"Sci Total Environ"},{"key":"2370_CR15","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.enconman.2008.09.001","volume":"50","author":"A Demirbas","year":"2009","unstructured":"Demirbas A (2009) Progress and recent trends in biodiesel fuels. Energy Convers Manag 50:14\u201334. https:\/\/doi.org\/10.1016\/j.enconman.2008.09.001","journal-title":"Energy Convers Manag"},{"key":"2370_CR16","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1007\/s11120-012-9761-6","volume":"113","author":"B Demmig-Adams","year":"2012","unstructured":"Demmig-Adams B, Cohu CM, Muller O et al (2012) Modulation of photosynthetic energy conversion efficiency in nature: from seconds to seasons. Photosynth Res 113:75\u201388. https:\/\/doi.org\/10.1007\/s11120-012-9761-6","journal-title":"Photosynth Res"},{"key":"2370_CR17","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1080\/09593330.2013.822523","volume":"35","author":"PD Gressler","year":"2014","unstructured":"Gressler PD, Bjerk TR, Schneider RdeC et al (2014) Cultivation of Desmodesmus subspicatus in a tubular photobioreactor for bioremediation and microalgae oil production. Environ Technol 35:209\u2013219. https:\/\/doi.org\/10.1080\/09593330.2013.822523","journal-title":"Environ Technol"},{"key":"2370_CR18","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/j.jclepro.2015.12.040","volume":"115","author":"SK Gupta","year":"2016","unstructured":"Gupta SK, Ansari FA, Shriwastav A et al (2016) Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for bio-fuels. J Clean Prod 115:255\u2013264. https:\/\/doi.org\/10.1016\/j.jclepro.2015.12.040","journal-title":"J Clean Prod"},{"key":"2370_CR19","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1016\/j.plipres.2006.01.001","volume":"45","author":"IA Guschina","year":"2006","unstructured":"Guschina IA, Harwood JL (2006) Lipids and lipid metabolism in eukaryotic algae. Prog Lipid Res 45:160\u2013186. https:\/\/doi.org\/10.1016\/j.plipres.2006.01.001","journal-title":"Prog Lipid Res"},{"key":"2370_CR20","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1007\/s00449-016-1585-x","volume":"39","author":"SF Han","year":"2016","unstructured":"Han SF, Jin W, Tu R et al (2016) Optimization of aeration for biodiesel production by Scenedesmus obliquus grown in municipal wastewater. Bioprocess Biosyst Eng 39:1073\u20131079. https:\/\/doi.org\/10.1007\/s00449-016-1585-x","journal-title":"Bioprocess Biosyst Eng"},{"key":"2370_CR21","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/j.ijheh.2011.10.003","volume":"215","author":"MA Hanjra","year":"2012","unstructured":"Hanjra MA, Blackwell J, Carr G et al (2012) Wastewater irrigation and environmental health: implications for water governance and public policy. Int J Hyg Environ Health 215:255\u2013269. https:\/\/doi.org\/10.1016\/j.ijheh.2011.10.003","journal-title":"Int J Hyg Environ Health"},{"key":"2370_CR22","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1016\/j.ecoenv.2019.02.052","volume":"174","author":"A Hern\u00e1ndez-Garc\u00eda","year":"2019","unstructured":"Hern\u00e1ndez-Garc\u00eda A, Vel\u00e1squez-Orta SB, Novelo E et al (2019) Wastewater-leachate treatment by microalgae: biomass, carbohydrate and lipid production. Ecotoxicol Environ Saf 174:435\u2013444. https:\/\/doi.org\/10.1016\/j.ecoenv.2019.02.052","journal-title":"Ecotoxicol Environ Saf"},{"key":"2370_CR23","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1111\/j.1365-313X.2008.03492.x","volume":"54","author":"Q Hu","year":"2008","unstructured":"Hu Q, Sommerfeld M, Jarvis E et al (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54:621\u2013639. https:\/\/doi.org\/10.1111\/j.1365-313X.2008.03492.x","journal-title":"Plant J"},{"key":"2370_CR24","doi-asserted-by":"publisher","first-page":"1207","DOI":"10.1080\/10934529.2018.1528032","volume":"53","author":"K Hu","year":"2018","unstructured":"Hu K, Zhao QL, Chen W et al (2018) Appropriate technologies for upgrading wastewater treatment plants: methods review and case studies in China. J Environ Sci Health A Tox Hazard Subst Environ Eng 53:1207\u20131220. https:\/\/doi.org\/10.1080\/10934529.2018.1528032","journal-title":"J Environ Sci Health A Tox Hazard Subst Environ Eng"},{"key":"2370_CR25","doi-asserted-by":"publisher","first-page":"104228","DOI":"10.1016\/j.algal.2025.104228","volume":"91","author":"R Jayabal","year":"2025","unstructured":"Jayabal R (2025) Microalgae-bacteria systems in wastewater treatment and biofuel production: Challenges, advances, and future perspectives. Algal Res 91:104228. https:\/\/doi.org\/10.1016\/j.algal.2025.104228","journal-title":"Algal Res"},{"key":"2370_CR26","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1016\/j.biortech.2015.09.037","volume":"198","author":"A Jebali","year":"2015","unstructured":"Jebali A, Aci\u00e9n FG, G\u00f3mez C et al (2015) Selection of native Tunisian microalgae for simultaneous wastewater treatment and biofuel production. Bioresour Technol 198:424\u2013430. https:\/\/doi.org\/10.1016\/j.biortech.2015.09.037","journal-title":"Bioresour Technol"},{"key":"2370_CR27","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1007\/s12257-008-0144-y","volume":"13","author":"G-T Jeong","year":"2008","unstructured":"Jeong G-T, Park J-H, Park S-H et al (2008) Estimating and improving cold filter plugging points by blending biodiesels with different fatty acid contents. Biotechnol Bioprocess Eng 13:505\u2013510. https:\/\/doi.org\/10.1007\/s12257-008-0144-y","journal-title":"Biotechnol Bioprocess Eng"},{"key":"2370_CR28","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.biortech.2014.09.144","volume":"184","author":"F Ji","year":"2015","unstructured":"Ji F, Zhou Y, Pang A et al (2015a) Fed-batch cultivation of Desmodesmus sp. in anaerobic digestion wastewater for improved nutrient removal and biodiesel production. Bioresour Technol 184:116\u2013122. https:\/\/doi.org\/10.1016\/j.biortech.2014.09.144","journal-title":"Bioresour Technol"},{"key":"2370_CR29","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/j.jenvman.2015.05.037","volume":"159","author":"MK Ji","year":"2015","unstructured":"Ji MK, Yun HS, Park YT et al (2015b) Mixotrophic cultivation of a microalga Scenedesmus obliquus in municipal wastewater supplemented with food wastewater and flue gas CO2 for biomass production. J Environ Manag 159:115\u2013120. https:\/\/doi.org\/10.1016\/j.jenvman.2015.05.037","journal-title":"J Environ Manag"},{"key":"2370_CR30","doi-asserted-by":"publisher","first-page":"103903","DOI":"10.1016\/j.algal.2025.103903","volume":"86","author":"MN Keddar","year":"2025","unstructured":"Keddar MN, Ballesteros-G\u00f3mez A, Siles JA et al (2025) Lipid and lutein recovery from desertic microalgae in saline wastewater combined to abiotic stressors for sustainable antioxidant and biodiesel production. Algal Res 86:103903. https:\/\/doi.org\/10.1016\/j.algal.2025.103903","journal-title":"Algal Res"},{"key":"2370_CR31","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.biortech.2013.02.006","volume":"134","author":"AJ Klok","year":"2013","unstructured":"Klok AJ, Martens DE, Wijffels RH et al (2013) Simultaneous growth and neutral lipid accumulation in microalgae. Bioresour Technol 134:233\u2013243. https:\/\/doi.org\/10.1016\/j.biortech.2013.02.006","journal-title":"Bioresour Technol"},{"key":"2370_CR32","doi-asserted-by":"publisher","first-page":"1059","DOI":"10.1016\/j.fuproc.2004.11.002","volume":"86","author":"G Knothe","year":"2005","unstructured":"Knothe G (2005) Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters. Fuel Process Technol 86:1059\u20131070. https:\/\/doi.org\/10.1016\/j.fuproc.2004.11.002","journal-title":"Fuel Process Technol"},{"key":"2370_CR33","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1039\/B903941D","volume":"2","author":"G Knothe","year":"2009","unstructured":"Knothe G (2009) Improving biodiesel fuel properties by modifying fatty ester composition. Energy Environ Sci 2:759\u2013766. https:\/\/doi.org\/10.1039\/B903941D","journal-title":"Energy Environ Sci"},{"key":"2370_CR34","doi-asserted-by":"publisher","first-page":"5265","DOI":"10.1021\/ef300700v","volume":"26","author":"G Knothe","year":"2012","unstructured":"Knothe G (2012) Fuel properties of highly polyunsaturated fatty acid methyl esters. prediction of fuel properties of algal biodiesel. Energy Fuels 26:5265\u20135273. https:\/\/doi.org\/10.1021\/ef300700v","journal-title":"Energy Fuels"},{"key":"2370_CR35","doi-asserted-by":"publisher","first-page":"552","DOI":"10.1007\/BF02645752","volume":"63","author":"K Krisnangkura","year":"1986","unstructured":"Krisnangkura K (1986) A simple method for estimation of cetane index of vegetable oil methyl esters. J Am Oil Chem Soc 63:552\u2013553. https:\/\/doi.org\/10.1007\/BF02645752","journal-title":"J Am Oil Chem Soc"},{"key":"2370_CR36","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1016\/j.biortech.2015.12.020","volume":"202","author":"I Krustok","year":"2016","unstructured":"Krustok I, Odlare M, Truu J et al (2016) Inhibition of nitrification in municipal wastewater-treating photobioreactors: effect on algal growth and nutrient uptake. Bioresour Technol 202:238\u2013243. https:\/\/doi.org\/10.1016\/j.biortech.2015.12.020","journal-title":"Bioresour Technol"},{"key":"2370_CR37","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1186\/1754-6834-5-18","volume":"5","author":"A Lei","year":"2012","unstructured":"Lei A, Chen H, Shen G et al (2012) Expression of fatty acid synthesis genes and fatty acid accumulation in haematococcus pluvialis under different stressors. Biotechnol Biofuels 5:18. https:\/\/doi.org\/10.1186\/1754-6834-5-18","journal-title":"Biotechnol Biofuels"},{"key":"2370_CR38","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1016\/j.biortech.2015.01.005","volume":"180","author":"T Li","year":"2015","unstructured":"Li T, Gargouri M, Feng J et al (2015) Regulation of starch and lipid accumulation in a microalga Chlorella sorokiniana. Bioresour Technol 180:250\u2013257. https:\/\/doi.org\/10.1016\/j.biortech.2015.01.005","journal-title":"Bioresour Technol"},{"key":"2370_CR39","doi-asserted-by":"publisher","first-page":"128578","DOI":"10.1016\/j.chemosphere.2020.128578","volume":"273","author":"G Li","year":"2021","unstructured":"Li G, Zhang J, Li H et al (2021) Towards high-quality biodiesel production from microalgae using original and anaerobically-digested livestock wastewater. Chemosphere 273:128578. https:\/\/doi.org\/10.1016\/j.chemosphere.2020.128578","journal-title":"Chemosphere"},{"key":"2370_CR40","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1016\/j.biortech.2018.04.092","volume":"262","author":"L Liu","year":"2018","unstructured":"Liu L, Zhao Y, Jiang X et al (2018) Lipid accumulation of Chlorella pyrenoidosa under mixotrophic cultivation using acetate and ammonium. Bioresour Technol 262:342\u2013346. https:\/\/doi.org\/10.1016\/j.biortech.2018.04.092","journal-title":"Bioresour Technol"},{"key":"2370_CR41","doi-asserted-by":"publisher","first-page":"2739","DOI":"10.1080\/09593330.2018.1449903","volume":"40","author":"LZ Luo","year":"2019","unstructured":"Luo LZ, Shao Y, Luo S et al (2019) Nutrient removal from piggery wastewater by Desmodesmus sp.CHX1 and its cultivation conditions optimization. Environ Technol 40:2739\u20132746. https:\/\/doi.org\/10.1080\/09593330.2018.1449903","journal-title":"Environ Technol"},{"key":"2370_CR42","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2019\/1216305","volume":"2019","author":"J Mao","year":"2019","unstructured":"Mao J, Li Y, Li S et al (2019) Bufei Jianpi granules reduce quadriceps muscular cell apoptosis by improving mitochondrial function in rats with chronic obstructive pulmonary disease. Evid Based Complement Alternat Med 2019:1. https:\/\/doi.org\/10.1155\/2019\/1216305","journal-title":"Evid Based Complement Alternat Med"},{"key":"2370_CR43","doi-asserted-by":"publisher","first-page":"1195","DOI":"10.1080\/15226514.2016.1193466","volume":"18","author":"CC Mar","year":"2016","unstructured":"Mar CC, Fan Y, Li FL et al (2016) Bioremediation of wastewater from edible oil refinery factory using oleaginous microalga Desmodesmus sp. S1. Int J Phytoremediation 18:1195\u20131201. https:\/\/doi.org\/10.1080\/15226514.2016.1193466","journal-title":"Int J Phytoremediation"},{"key":"2370_CR44","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1016\/j.watres.2014.07.025","volume":"65","author":"G Markou","year":"2014","unstructured":"Markou G, Vandamme D, Muylaert K (2014) Microalgal and cyanobacterial cultivation: the supply of nutrients. Water Res 65:186\u2013202. https:\/\/doi.org\/10.1016\/j.watres.2014.07.025","journal-title":"Water Res"},{"key":"2370_CR45","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1093\/jxb\/51.345.659","volume":"51","author":"K Maxwell","year":"2000","unstructured":"Maxwell K, Johnson GN (2000) Chlorophyll fluorescence\u2013a practical guide. J Exp Bot 51:659\u2013668. https:\/\/doi.org\/10.1093\/jxb\/51.345.659","journal-title":"J Exp Bot"},{"key":"2370_CR46","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1111\/jpy.12367","volume":"52","author":"R Mera","year":"2016","unstructured":"Mera R, Torres E, Abalde J (2016) Effects of sodium sulfate on the freshwater microalga Chlamydomonas moewusii: implications for the optimization of algal culture media. J Phycol 52:75\u201388. https:\/\/doi.org\/10.1111\/jpy.12367","journal-title":"J Phycol"},{"key":"2370_CR47","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1016\/j.biombioe.2005.05.001","volume":"29","author":"M Mohibbe Azam","year":"2005","unstructured":"Mohibbe Azam M, Waris A, Nahar NM (2005) Prospects and potential of fatty acid methyl esters of some non-traditional seed oils for use as biodiesel in India. Biomass Bioenerg 29:293\u2013302. https:\/\/doi.org\/10.1016\/j.biombioe.2005.05.001","journal-title":"Biomass Bioenerg"},{"key":"2370_CR48","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/s13068-019-1646-x","volume":"13","author":"JC Nzayisenga","year":"2020","unstructured":"Nzayisenga JC, Farge X, Groll SL et al (2020a) Effects of light intensity on growth and lipid production in microalgae grown in wastewater. Biotechnol Biofuels 13:4. https:\/\/doi.org\/10.1186\/s13068-019-1646-x","journal-title":"Biotechnol Biofuels"},{"key":"2370_CR49","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25092107","author":"JC Nzayisenga","year":"2020","unstructured":"Nzayisenga JC, Niemi C, Ferro L et al (2020b) Screening suitability of northern hemisphere algal strains for heterotrophic cultivation and fatty acid methyl ester production. Molecules. https:\/\/doi.org\/10.3390\/molecules25092107","journal-title":"Molecules"},{"key":"2370_CR50","doi-asserted-by":"publisher","first-page":"1848","DOI":"10.1093\/pcp\/pcu113","volume":"55","author":"O Osundeko","year":"2014","unstructured":"Osundeko O, Dean AP, Davies H et al (2014) Acclimation of microalgae to wastewater environments involves increased oxidative stress tolerance activity. Plant Cell Physiol 55:1848\u20131857. https:\/\/doi.org\/10.1093\/pcp\/pcu113","journal-title":"Plant Cell Physiol"},{"key":"2370_CR51","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.watres.2010.08.037","volume":"45","author":"O Perez-Garcia","year":"2011","unstructured":"Perez-Garcia O, Escalante FME, de-Bashan LE et al (2011) Heterotrophic cultures of microalgae: metabolism and potential products. Water Res 45:11\u201336. https:\/\/doi.org\/10.1016\/j.watres.2010.08.037","journal-title":"Water Res"},{"key":"2370_CR52","doi-asserted-by":"publisher","first-page":"4207","DOI":"10.1016\/j.watres.2009.06.011","volume":"43","author":"N Powell","year":"2009","unstructured":"Powell N, Shilton A, Chisti Y et al (2009) Towards a luxury uptake process via microalgae-Defining the polyphosphate dynamics. Water Res 43:4207\u20134213. https:\/\/doi.org\/10.1016\/j.watres.2009.06.011","journal-title":"Water Res"},{"key":"2370_CR53","doi-asserted-by":"publisher","first-page":"103581","DOI":"10.1016\/j.algal.2024.103581","volume":"81","author":"NRH Rao","year":"2024","unstructured":"Rao NRH, Beyer V, Thielemans W et al (2024) Techno-economic comparison of flocculation combined with dissolved air flotation versus sedimentation for microalgae harvesting. Algal Res 81:103581. https:\/\/doi.org\/10.1016\/j.algal.2024.103581","journal-title":"Algal Res"},{"key":"2370_CR54","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1099\/00221287-111-1-1","volume":"111","author":"R Rippka","year":"1979","unstructured":"Rippka R, Deruelles J, Waterbury JB et al (1979) Generic assignments, strain histories and properties of pure cultures of Cyanobacteria. Microbiology 111:1\u201361. https:\/\/doi.org\/10.1099\/00221287-111-1-1","journal-title":"Microbiology"},{"key":"2370_CR55","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1007\/s10811-010-9591-x","volume":"23","author":"S Salim","year":"2011","unstructured":"Salim S, Bosma R, Vermu\u00eb MH et al (2011) Harvesting of microalgae by bio-flocculation. J Appl Phycol 23:849\u2013855. https:\/\/doi.org\/10.1007\/s10811-010-9591-x","journal-title":"J Appl Phycol"},{"key":"2370_CR56","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1016\/j.watres.2012.11.006","volume":"47","author":"G Samor\u00ec","year":"2013","unstructured":"Samor\u00ec G, Samor\u00ec C, Guerrini F et al (2013) Growth and nitrogen removal capacity of Desmodesmus communis and of a natural microalgae consortium in a batch culture system in view of urban wastewater treatment: part I. Water Res 47:791\u2013801. https:\/\/doi.org\/10.1016\/j.watres.2012.11.006","journal-title":"Water Res"},{"key":"2370_CR57","doi-asserted-by":"publisher","first-page":"2797","DOI":"10.1007\/s10311-022-01458-1","volume":"20","author":"A Sarwer","year":"2022","unstructured":"Sarwer A, Hamed SM, Osman AI et al (2022) Algal biomass valorization for biofuel production and carbon sequestration: a review. Environ Chem Lett 20:2797\u20132851. https:\/\/doi.org\/10.1007\/s10311-022-01458-1","journal-title":"Environ Chem Lett"},{"key":"2370_CR58","doi-asserted-by":"publisher","first-page":"28004","DOI":"10.1007\/s11356-018-2834-0","volume":"25","author":"E Sforza","year":"2018","unstructured":"Sforza E, Pastore M, Spagni A et al (2018) Microalgae-bacteria gas exchange in wastewater: How mixotrophy may reduce the oxygen supply for bacteria. Environ Sci Pollut Res 25:28004\u201328014. https:\/\/doi.org\/10.1007\/s11356-018-2834-0","journal-title":"Environ Sci Pollut Res"},{"key":"2370_CR59","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1039\/d4va00136b","volume":"4","author":"J Shamshad","year":"2024","unstructured":"Shamshad J, Ur Rehman R (2024) Innovative approaches to sustainable wastewater treatment: a comprehensive exploration of conventional and emerging technologies. Environ Sci Adv 4:189\u2013222. https:\/\/doi.org\/10.1039\/d4va00136b","journal-title":"Environ Sci Adv"},{"key":"2370_CR60","doi-asserted-by":"publisher","first-page":"e0224926","DOI":"10.1371\/journal.pone.0224926","volume":"14","author":"Q Shao","year":"2019","unstructured":"Shao Q, Hu L, Qin H et al (2019) Metabolomic response of Euglena gracilis and its bleached mutant strain to light. PLoS ONE 14:e0224926. https:\/\/doi.org\/10.1371\/journal.pone.0224926","journal-title":"PLoS ONE"},{"key":"2370_CR61","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1111\/jpy.12549","volume":"53","author":"R Sivaramakrishnan","year":"2017","unstructured":"Sivaramakrishnan R, Incharoensakdi A (2017) Enhancement of total lipid yield by nitrogen, carbon, and iron supplementation in isolated microalgae. J Phycol 53:855\u2013868. https:\/\/doi.org\/10.1111\/jpy.12549","journal-title":"J Phycol"},{"key":"2370_CR62","volume-title":"Monitoring Method of Water and Wastewater","author":"State Environmental Protection Administration","year":"2002","unstructured":"State Environmental Protection Administration (2002) Monitoring Method of Water and Wastewater, 4th edn. China Environmental Science Press, Beijing.","edition":"4"},{"key":"2370_CR63","doi-asserted-by":"publisher","first-page":"1462916","DOI":"10.1155\/2016\/1462916","volume":"2016","author":"SM Sun","year":"2016","unstructured":"Sun SM, Yang SH, Golokhvast KS et al (2016) Reconstructing the Phylogeny of Capsosiphon fulvescens (Ulotrichales, Chlorophyta) from Korea Based on rbcL and 18S rDNA Sequences. Biomed Res Int 2016:1462916. https:\/\/doi.org\/10.1155\/2016\/1462916","journal-title":"Biomed Res Int"},{"key":"2370_CR64","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/S0269-7491(99)00118-9","volume":"107","author":"NFY Tam","year":"2000","unstructured":"Tam NFY, Wong YS (2000) Effect of immobilized microalgal bead concentrations on wastewater nutrient removal. Environ Pollut 107:145\u2013151. https:\/\/doi.org\/10.1016\/S0269-7491(99)00118-9","journal-title":"Environ Pollut"},{"key":"2370_CR65","doi-asserted-by":"publisher","first-page":"132110","DOI":"10.1016\/j.biortech.2025.132110","volume":"421","author":"HT Thanh Pham","year":"2025","unstructured":"Thanh Pham HT, Kuroda S, Khairina Y et al (2025) Creation of a functional duckweed holobiont to reduce nutrient competition with microalgae for high-yield biomass production. Bioresour Technol 421:132110. https:\/\/doi.org\/10.1016\/j.biortech.2025.132110","journal-title":"Bioresour Technol"},{"key":"2370_CR66","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.tibtech.2012.12.005","volume":"31","author":"D Vandamme","year":"2013","unstructured":"Vandamme D, Foubert I, Muylaert K (2013) Flocculation as a low-cost method for harvesting microalgae for bulk biomass production. Trends Biotechnol 31:233\u2013239. https:\/\/doi.org\/10.1016\/j.tibtech.2012.12.005","journal-title":"Trends Biotechnol"},{"issue":"16","key":"2370_CR67","doi-asserted-by":"publisher","first-page":"7553","DOI":"10.3390\/su17167553","volume":"17","author":"S Vaz","year":"2025","unstructured":"Vaz S, Martins R, Pinheiro HM et al (2025) Effect of hydraulic retention time on nutrient removal in a microalgae-based tertiary treatment: a pilot-scale study in winter conditions. Sustainability 17(16):7553. https:\/\/doi.org\/10.3390\/su17167553","journal-title":"Sustainability"},{"key":"2370_CR68","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.watres.2017.03.030","volume":"116","author":"J V\u00f6lker","year":"2017","unstructured":"V\u00f6lker J, Vogt T, Castronovo S et al (2017) Extended anaerobic conditions in the biological wastewater treatment: higher reduction of toxicity compared to target organic micropollutants. Water Res 116:220\u2013230. https:\/\/doi.org\/10.1016\/j.watres.2017.03.030","journal-title":"Water Res"},{"key":"2370_CR69","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.biortech.2013.12.047","volume":"154","author":"M Wang","year":"2014","unstructured":"Wang M, Kuo-Dahab WC, Dolan S et al (2014) Kinetics of nutrient removal and expression of extracellular polymeric substances of the microalgae, Chlorella sp. and Micractinium sp., in wastewater treatment. Bioresour Technol 154:131\u2013137. https:\/\/doi.org\/10.1016\/j.biortech.2013.12.047","journal-title":"Bioresour Technol"},{"key":"2370_CR70","doi-asserted-by":"publisher","first-page":"1460675","DOI":"10.3389\/fnut.2024.1460675","volume":"11","author":"S Yuan","year":"2024","unstructured":"Yuan S, Du M, Li X et al (2024) Adaptability and nutritional analysis of a newly isolated Chlorella sp. NeZha in brackish and marine environments with potential bioeconomic impacts. Front Nutr 11:1460675. https:\/\/doi.org\/10.3389\/fnut.2024.1460675","journal-title":"Front Nutr"},{"key":"2370_CR71","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.envint.2016.03.024","volume":"92-93","author":"QH Zhang","year":"2016","unstructured":"Zhang QH, Yang WN, Ngo HH et al (2016) Current status of urban wastewater treatment plants in China. Environ Int 92\u201393:11\u201322. https:\/\/doi.org\/10.1016\/j.envint.2016.03.024","journal-title":"Environ Int"},{"key":"2370_CR72","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ecoleng.2017.10.008","volume":"110","author":"H Znad","year":"2018","unstructured":"Znad H, Al Ketife AMD, Judd S et al (2018) Bioremediation and nutrient removal from wastewater by Chlorella vulgaris. Ecol Eng 110:1\u20137. https:\/\/doi.org\/10.1016\/j.ecoleng.2017.10.008","journal-title":"Ecol Eng"},{"key":"2370_CR73","doi-asserted-by":"publisher","first-page":"131450","DOI":"10.1016\/j.biortech.2024.131450","volume":"413","author":"R Zulekha","year":"2024","unstructured":"Zulekha R, Mubashar M, Muzamil Sultan M et al (2024) An assessment of the autotrophic\/heterotrophic synergism in microalgae under mixotrophic mode and its contribution in high-rate phosphate recovery from wastewater. Bioresour Technol 413:131450. https:\/\/doi.org\/10.1016\/j.biortech.2024.131450","journal-title":"Bioresour Technol"}],"container-title":["Antonie van Leeuwenhoek"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10482-026-02370-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10482-026-02370-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10482-026-02370-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T13:46:22Z","timestamp":1785764782000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10482-026-02370-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,16]]},"references-count":73,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["2370"],"URL":"https:\/\/doi.org\/10.1007\/s10482-026-02370-2","relation":{},"ISSN":["0003-6072","1572-9699"],"issn-type":[{"value":"0003-6072","type":"print"},{"value":"1572-9699","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,16]]},"assertion":[{"value":"12 November 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 June 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All authors declare that they have no conflicts of interest.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Ethics declaration: not applicable. This study did not involve human or animal subjects, and therefore no ethical approval was required.","order":2,"name":"Ethics","label":"Ethics approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study did not involve human subjects.","order":3,"name":"Ethics","label":"Consent to participate","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study did not involve human subjects. All authors confirm their consent to the publication of all data in this manuscript.","order":4,"name":"Ethics","label":"Consent to publish","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"166"}}