{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T07:10:13Z","timestamp":1773904213199,"version":"3.50.1"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2012,11,7]],"date-time":"2012-11-07T00:00:00Z","timestamp":1352246400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Appl Acarol"],"published-print":{"date-parts":[[2013,4]]},"DOI":"10.1007\/s10493-012-9611-4","type":"journal-article","created":{"date-parts":[[2012,11,6]],"date-time":"2012-11-06T10:58:51Z","timestamp":1352199531000},"page":"473-481","source":"Crossref","is-referenced-by-count":21,"title":["Detection of Leishmania infantum in animals and their ectoparasites by conventional PCR and real time PCR"],"prefix":"10.1007","volume":"59","author":[{"given":"Rayana Carla Silva","family":"de Morais","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su\u00eania da Cunha","family":"Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pietra Lemos","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamila Gaud\u00eancio","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Jos\u00e9","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00f4mulo Pessoa e","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Edileuza Felinto","family":"de Brito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sinval Pinto","family":"Brand\u00e3o-Filho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filipe","family":"Dantas-Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milena","family":"de Paiva-Cavalcanti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,11,7]]},"reference":[{"key":"9611_CR1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1590\/S0074-02761961000200001","volume":"59","author":"HB Arag\u00e3o","year":"1961","unstructured":"Arag\u00e3o HB, Fonseca F (1961) Notas de Ixodologia VIII. Lista e chave para representantes da fauna de Ixodol\u00f3gica Brasileira. Mem Inst Oswaldo Cruz 59:115\u2013129","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"9611_CR2","volume-title":"BIOESTAT: Aplica\u00e7\u00f5es estat\u00edsticas nas \u00e1reas das ci\u00eancias bio-m\u00e9dicas","author":"M Ayres","year":"2007","unstructured":"Ayres M, Ayres J\u00fanior M, Ayres DL, Santos AA (2007) BIOESTAT: Aplica\u00e7\u00f5es estat\u00edsticas nas \u00e1reas das ci\u00eancias bio-m\u00e9dicas. Sociedade Civil Mamirau\u00e1, Bel\u00e9m"},{"key":"9611_CR3","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1016\/j.cvsm.2009.07.001","volume":"39","author":"LB Blagburn","year":"2009","unstructured":"Blagburn LB, Dryden WM (2009) Biology, treatment, and control of flea and tick infestations. Vet Clin North Am Small Anim Pract 39:1173\u20131200","journal-title":"Vet Clin North Am Small Anim Pract"},{"key":"9611_CR4","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1017\/S0031182011002198","volume":"139","author":"E Brianti","year":"2012","unstructured":"Brianti E, Gaglio G, Napoli E, Giannetto S, Dantas-Torres F, Bain O, Otranto D (2012) New insights into the ecology and biology of Acanthocheilonema reconditum (Grassi, 1889) causing canine subcutaneous filariosis. Parasitology 139:530\u2013536","journal-title":"Parasitology"},{"key":"9611_CR5","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1038\/nrmicro1748","volume":"5","author":"F Chappuis","year":"2007","unstructured":"Chappuis F, Sundar S, Hailu A, Ghalib H, Rijal S, Peeling RW, Alvar J, Boelaert M (2007) Visceral leishmaniasis: what are the needs for diagnosis, treatment and control? Nat Rev Microbiol 5:873\u2013882","journal-title":"Nat Rev Microbiol"},{"key":"9611_CR6","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.vetpar.2010.10.052","volume":"176","author":"WD Coelho","year":"2011","unstructured":"Coelho WD, Pereira BBR, Langoni H, Bresciani KDS (2011) Molecular detection of Leishmania spp. in cats (Felis catus) from Andradina Municipality, S\u00e3o Paulo State, Brazil. Vet Parasitol 176:281\u2013282","journal-title":"Vet Parasitol"},{"key":"9611_CR7","first-page":"213","volume":"47","author":"TAC Costa","year":"2010","unstructured":"Costa TAC, Rossi CN, Laurenti MD, Gomes AAD, Vides JP, Sobrinho LSV, Marcondes M (2010) Occurrence of leishmaniosis in cats of an endemic area for visceral leishmaniosis. Braz J Vet Anim Sci 47:213\u2013217","journal-title":"Braz J Vet Anim Sci"},{"key":"9611_CR8","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.vetpar.2007.04.008","volume":"147","author":"MTZ Coutinho","year":"2007","unstructured":"Coutinho MTZ, Linardi PM (2007) Can fleas from dogs infected with canine visceral leishmaniasis transfer the infection to other mammals? Vet Parasitol 147:320\u2013325","journal-title":"Vet Parasitol"},{"key":"9611_CR9","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.vetpar.2004.11.011","volume":"128","author":"MT Coutinho","year":"2005","unstructured":"Coutinho MT, Bueno LL, Sterzik A, Fujiwara RT, Botelho JR, Maria M, Genaro O, Linardi PM (2005) Participation of Rhipicephalus sanguineus (Acari: Ixodidae) in the epidemiology of canine visceral leishmaniasis. Vet Parasitol 128:149\u2013155","journal-title":"Vet Parasitol"},{"key":"9611_CR11","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.vetpar.2007.07.007","volume":"149","author":"F Dantas-Torres","year":"2007","unstructured":"Dantas-Torres F (2007) The role of dogs as reservoir of Leishmania parasites, with emphasis on Leishmania (Leishmania) infantum and Leishmania (Viannia) braziliensis. Vet Parasitol 149:139\u2013146","journal-title":"Vet Parasitol"},{"key":"9611_CR12","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.vetpar.2007.12.030","volume":"152","author":"F Dantas-Torres","year":"2008","unstructured":"Dantas-Torres F (2008) The brown dog tick, Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae): from taxonomy to control. Vet Parasitol 152:173\u2013185","journal-title":"Vet Parasitol"},{"key":"9611_CR13","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.pt.2010.12.006","volume":"27","author":"F Dantas-Torres","year":"2011","unstructured":"Dantas-Torres F (2011) Ticks as vectors of Leishmania parasites. Trends Parasitol 27:155\u2013159","journal-title":"Trends Parasitol"},{"key":"9611_CR14","doi-asserted-by":"crossref","first-page":"77","DOI":"10.11606\/issn.1678-4456.bjvras.2007.26644","volume":"44","author":"F Dantas-Torres","year":"2007","unstructured":"Dantas-Torres F, Figueredo LA (2007) Heterodoxus spiniger (Enderlein, 1909) on domestic dogs (Canis familiaris, L. 1758) from the city of Recife, Pernambuco State, Brazil. Braz J Vet Res Anim Sci 44:77\u201380","journal-title":"Braz J Vet Res Anim Sci"},{"key":"9611_CR15","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1186\/1756-3305-4-56","volume":"4","author":"F Dantas-Torres","year":"2011","unstructured":"Dantas-Torres F, Latrofa MS, Otranto D (2011) Quantification of Leishmania infantum DNA in females, eggs and larvae of Rhipicephalus sanguineus. Parasit Vectors 4:56","journal-title":"Parasit Vectors"},{"key":"9611_CR16","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.ijpara.2010.12.008","volume":"41","author":"AM Dougall","year":"2011","unstructured":"Dougall AM, Alexander B, Holt DC, Harris T, Sultan AH, Bates PA, Rose K, Walton SF (2011) Evidence incriminating midges (Diptera: Ceratopogonidae) as potential vectors of Leishmania in Australia. Int J Patasitol 41:571\u2013579","journal-title":"Int J Patasitol"},{"key":"9611_CR17","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.vetpar.2009.06.026","volume":"165","author":"MGPA Ferreira","year":"2009","unstructured":"Ferreira MGPA, Fatorri KR, Souza F, Lima VMF (2009) Potential role for dog fleas in the cycle of Leishmania spp. Vet Parasitol 165:150\u2013154","journal-title":"Vet Parasitol"},{"key":"9611_CR18","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.vetpar.2006.01.011","volume":"137","author":"O Francino","year":"2006","unstructured":"Francino O, Altet L, Sanchez-Robert E, Rodriguez A, Solano-Gallego L, Alberola J, Ferrer L, S\u00e1nchez A, Roura X (2006) Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniosis. Vet Parasitol 137:214\u2013221","journal-title":"Vet Parasitol"},{"key":"9611_CR19","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.vetpar.2006.10.008","volume":"144","author":"AHS Gomes","year":"2007","unstructured":"Gomes AHS, Ferreira IMR, Lima MLSR, Cunha EA, Garcia AS, Ara\u00fajo MFL, Pereira-Chioccola VL (2007) PCR identification of Leishmania in diagnosis and control of canine leishmaniasis. Vet Parasitol 144:234\u2013241","journal-title":"Vet Parasitol"},{"key":"9611_CR20","volume-title":"Ectoparasitos de import\u00e2ncia veterin\u00e1ria","author":"JH Guimar\u00e3es","year":"2011","unstructured":"Guimar\u00e3es JH, Tucci EC, Barros-Battesti DM (2011) Ectoparasitos de import\u00e2ncia veterin\u00e1ria. Pl\u00eaiade\/FAPESP, S\u00e3o Paulo"},{"key":"9611_CR21","first-page":"449","volume-title":"The chewing lice: world checklist and biological overview (Special Publication 24)","author":"KP Johnson","year":"2003","unstructured":"Johnson KP, Clayton DH (2003) The biology, ecology, and evolution of chewing lice. In: Price RD, Hellenthal RA, Palma RL, Johnson KP, Clayton DH (eds) The chewing lice: world checklist and biological overview (Special Publication 24). Illinois Natural History Survey, Champaign, pp 449\u2013476"},{"key":"9611_CR22","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.pt.2011.03.008","volume":"27","author":"C Maia","year":"2011","unstructured":"Maia C, Campino L (2011) Can domestic cats be considered reservoir hosts of zoonotic leishmaniasis? Trends Parasitol 27:341\u2013344","journal-title":"Trends Parasitol"},{"key":"9611_CR23","unstructured":"Mckenzie KK (1984) A study of the transmission of canine leishmaniasis by the tick, Rhipicephalus sanguineus, and an ultrastructural comparison of the promastigote. PhD Dissertation, Oklahoma State University"},{"key":"9611_CR24","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1016\/S0140-6736(05)67629-5","volume":"366","author":"HW Murray","year":"2005","unstructured":"Murray HW, Berman JD, Davies CR, Saravia NG (2005) Advances in leishmaniasis. Lancet 366:1561\u20131577","journal-title":"Lancet"},{"key":"9611_CR25","first-page":"5","volume":"16","author":"CM Nunes","year":"2007","unstructured":"Nunes CM, Dias AKK, Gottardi FP, Paula HB, Azevedo MAA, Lima VMF (2007) Avalia\u00e7\u00e3o da rea\u00e7\u00e3o em cadeia da polimerase para diagn\u00f3stico da leishmaniose visceral em sangue de c\u00e3es. Rev Bras Parasitol Vet 16:5\u20139","journal-title":"Rev Bras Parasitol Vet"},{"key":"9611_CR26","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.vetpar.2009.11.016","volume":"168","author":"D Otranto","year":"2010","unstructured":"Otranto D, Dantas-Torres F (2010) Fleas and ticks as vectors of Leishmania spp. to dogs: caution is needed. Vet Parasitol 168:173\u2013174","journal-title":"Vet Parasitol"},{"key":"9611_CR27","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.tvjl.2008.05.018","volume":"189","author":"M Paiva-Cavalcanti","year":"2009","unstructured":"Paiva-Cavalcanti M, Brito MEF, Souza WV, Gomes YM, Abath FG (2009) The development of a real-time PCR assay for the quantification of Leishmania infantum DNA in canine blood. Vet J 189:356\u2013358","journal-title":"Vet J"},{"key":"9611_CR28","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1590\/S1678-91992010000400004","volume":"16","author":"M Paiva-Cavalcanti","year":"2010","unstructured":"Paiva-Cavalcanti M, Regis-da-Silva CG, Gomes YM (2010) Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review. J Venomous Anim Toxins Incl Trop Dis 16:537\u2013542","journal-title":"J Venomous Anim Toxins Incl Trop Dis"},{"key":"9611_CR29","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.prevetmed.2010.08.006","volume":"97","author":"GF Paz","year":"2010","unstructured":"Paz GF, Ribeiro MFB, Magalh\u00e3es DF, Sathler KPB, Morais MHF, Fi\u00faza VOP, Brand\u00e3o ST, Weneck GL, Fortes-Dias CL, Dias ES (2010) Association between the prevalence of infestation by Rhipicephalus Sanguineus and Ctenocephalides felis felis and the presence of Anti-Leishmania antibodies: a case-control study in dogs from a Brazilian endemic area. Prev Vet Med 97:131\u2013133","journal-title":"Prev Vet Med"},{"key":"9611_CR30","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev.ento.42.1.451","volume":"42","author":"M Rust","year":"1997","unstructured":"Rust M, Dryden M (1997) The biology, ecology and management of the cat flea. Ann Rev Entomol 42:451\u2013473","journal-title":"Ann Rev Entomol"},{"key":"9611_CR31","first-page":"49","volume":"100","author":"OA Silva","year":"2007","unstructured":"Silva OA, Silva PB, Silva OV, Braga GM, Albuquerque J\u00fanior A, Queiros Neto V, Rocha ME, Silva EF (2007) Canine visceral leishmaniasis in northeast Brazil: epidemiological aspects. Bull Soc Pathol Exot 100:49\u201350","journal-title":"Bull Soc Pathol Exot"},{"key":"9611_CR32","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.vetpar.2010.12.042","volume":"178","author":"JP Vides","year":"2011","unstructured":"Vides JP, Schwardt TF, Sobrinho LSV, Marinho M, Laurenti MD, Biondo AW, Leutenegger C, Marcondes M (2011) Leishmania chagasi infection in cats with dermatologic lesions from an endemic area of visceral leishmaniasis in Brazil. Vet Parasitol 178:22\u201328","journal-title":"Vet Parasitol"},{"key":"9611_CR33","unstructured":"World Health Organization (2012) Leishmaniasis\u2013burden of disease. http:\/\/www.who.int\/leishmaniasis\/burden\/en\/ . Accessed 15 may 2012"},{"key":"9611_CR34","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/S1473-3099(04)01044-8","volume":"4","author":"S Yang","year":"2004","unstructured":"Yang S, Rothman RE (2004) PCR-based diagnostics for infectious diseases: uses, limitations and future applications in acute-care settings. Lancet Infect Dis 4:337\u2013348","journal-title":"Lancet Infect Dis"}],"container-title":["Experimental and Applied Acarology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10493-012-9611-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10493-012-9611-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10493-012-9611-4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,29]],"date-time":"2019-05-29T18:27:21Z","timestamp":1559154441000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10493-012-9611-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,7]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["9611"],"URL":"https:\/\/doi.org\/10.1007\/s10493-012-9611-4","relation":{},"ISSN":["0168-8162","1572-9702"],"issn-type":[{"value":"0168-8162","type":"print"},{"value":"1572-9702","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,7]]}}}