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We conducted a study to identify tick species infesting slaughter bulls from Adama City and to screen them for tick-borne pathogens. In 2016, 291 ticks were collected from 37 bulls in Adama, which were ready for slaughter. Ticks were identified morphologically. Total genomic DNA was extracted from ticks and used to test for <jats:italic>Rickettsia<\/jats:italic> spp. with real-time PCR. Species identification was done by phylogenetic analysis using sequencing that targeted the 23S-5S intergenic spacer region and <jats:italic>ompA<\/jats:italic> genes. Four tick species from two genera, <jats:italic>Amblyomma<\/jats:italic> and <jats:italic>Rhipicephalus<\/jats:italic>, were identified. <jats:italic>Amblyomma cohaerens<\/jats:italic> was the dominant species (n\u2009=\u2009241, 82.8%), followed by <jats:italic>Amblyomma variegatum<\/jats:italic> (n\u2009=\u200922, 7.5%), <jats:italic>Rhipicephalus pulchellus<\/jats:italic> (n\u2009=\u200919, 6.5%), and <jats:italic>Rhipicephalus decoloratus<\/jats:italic> (n\u2009=\u20099, 3.0%). Among all ticks, 32 (11%) were positive for <jats:italic>Rickettsia<\/jats:italic> spp. and 15 (5.2%) of these were identified as <jats:italic>R. africae<\/jats:italic> comprising at least two genetic clades, occurring in <jats:italic>A. variegatum<\/jats:italic> (n\u2009=\u200910) and <jats:italic>A. cohaerens<\/jats:italic> (n\u2009=\u20095). The remainder of <jats:italic>Rickettsia<\/jats:italic>-positive samples could not be amplified due to low DNA yield. Furthermore, another 15 (5.2%) samples carried other pathogenic bacteria: <jats:italic>Ehrlichia ruminantium<\/jats:italic> (n\u2009=\u20099; 3.1%) in <jats:italic>A. cohaerens<\/jats:italic>, <jats:italic>Ehrlichia<\/jats:italic> sp. (n\u2009=\u20093; 1%) in <jats:italic>Rh. pulchellus<\/jats:italic> and <jats:italic>A. cohaerens<\/jats:italic>, <jats:italic>Anaplasma<\/jats:italic> sp. (n\u2009=\u20091; 0.5%) in <jats:italic>A. cohaerens<\/jats:italic>, and <jats:italic>Neoehrlichia mikurensis<\/jats:italic> (n\u2009=\u20092; 0.7%) in <jats:italic>A. cohaerens.<\/jats:italic> All ticks were negative for <jats:italic>Bartonella<\/jats:italic> spp., <jats:italic>Babesia<\/jats:italic> spp., <jats:italic>Theileria<\/jats:italic> spp., and <jats:italic>Hepatozoon<\/jats:italic> spp. We reported for the first time <jats:italic>E. ruminatium<\/jats:italic>, <jats:italic>N. mikurensis<\/jats:italic>, <jats:italic>Ehrlichia<\/jats:italic> sp., and <jats:italic>Anaplasma<\/jats:italic> sp. in <jats:italic>A. cohaerens.<\/jats:italic> Medically and veterinarily important pathogens were mostly detected from <jats:italic>A. variegatum<\/jats:italic> and <jats:italic>A. cohaerens.<\/jats:italic> These data are relevant for a One-health approach for monitoring and prevention of tick-borne disease transmission.<\/jats:p>","DOI":"10.1007\/s10493-021-00623-5","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T15:59:09Z","timestamp":1619625549000},"page":"459-471","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Tick species from cattle in the Adama Region of Ethiopia and pathogens detected"],"prefix":"10.1007","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9505-9341","authenticated-orcid":false,"given":"Tafese Beyene","family":"Tufa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"W\u00f6lfel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Zubrikov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bronislava","family":"V\u00edchov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramona","family":"Rie\u00df","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliana","family":"Rutaihwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Fuchs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans Martin","family":"Orth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"H\u00e4ussinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"Feldt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven","family":"Poppert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Dobler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deon K.","family":"Bakkes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidia","family":"Chitimia-Dobler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,28]]},"reference":[{"issue":"3","key":"623_CR1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","volume":"215","author":"SF Altschul","year":"1990","unstructured":"Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. 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