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Schwarz &amp; P.H. Davis and <jats:italic>Dorystoechas hastata<\/jats:italic> Boiss. &amp; Heldr. ex Bentham, both endemic to Turkey, on <jats:italic>Rhipicephalus sanguineus<\/jats:italic> s.l. Latreille (Acari: Ixodidae). The study also introduces a new test method that can be used to assess the repellent effects against ticks. Both plant EOs exhibited the highest larvicidal activity against brown dog tick larvae after 24\u00a0h and LC<jats:sub>50<\/jats:sub> and LC<jats:sub>90<\/jats:sub> values were determined as 0.101% and 0.125% for <jats:italic>O. minutiflorum<\/jats:italic> essential oil and 0.937% and 2.1% for <jats:italic>D. hastata<\/jats:italic> essential oil, respectively. In this study, we have described a detailed protocol for a novel larval repellent activity test (LRAT) for essential oils and extracts, using simple equipment. The advantages and limitations of LRAT, when compared to other tests commonly used to determine repellent effect against ticks, are also included in this study. The LRAT was developed with modifications of the larval immersion test (LIT) and proves to be a highly efficient and easily observable method. It can be used to test any active substance that may be toxic to humans and animals. According to the LRAT, at the end of 3\u00a0h, <jats:italic>O. minutiflorum<\/jats:italic> essential oil showed a high repellent effect, varying between 84.14% and 100% at 1% concentration. This result was not statistically different from the DEET, the positive control. When comparing the larvicidal and repellent activities, <jats:italic>O. minutiflorum<\/jats:italic> essential oil was found to be more effective than <jats:italic>D. hastata<\/jats:italic> essential oil.<\/jats:p>","DOI":"10.1007\/s10493-023-00892-2","type":"journal-article","created":{"date-parts":[[2024,2,13]],"date-time":"2024-02-13T17:02:19Z","timestamp":1707843739000},"page":"263-273","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Larvicidal and repellent effects of essential oils on the brown dog tick (Rhipicephalus sanguineus Sensu lato) with description of new larval repellent activity test method"],"prefix":"10.1007","volume":"92","author":[{"given":"Samed","family":"Koc","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeynep Nur","family":"Gultekin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sevval","family":"Kahraman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aysegul","family":"Cengiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burak","family":"Polat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cansu","family":"Caliskan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ozge","family":"Tufan-Cetin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huseyin","family":"Cetin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,13]]},"reference":[{"key":"892_CR1","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1515\/ovs-2020-0102","volume":"1","author":"OA Adekoya","year":"2020","unstructured":"Adekoya OA, Adenubi OT, Adebayo OO, Adebowale OO, Oyewusi JA, Olukunle JO (2020) Retrospective study of suspected canine poisoning cases at the Veterinary Teaching Hospital, Federal University of Agriculture, Abeokuta, Nigeria (2009\u20132019). 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