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The present study aimed to evaluate the status of these pathogens in\n                    <jats:italic>H. dromedarii<\/jats:italic>\n                    ticks over four seasons of a year, in addition to investigate the infections of piroplasms and Anaplasmataceae besides their genetic diversity starting from June 2021 till April 2022. A total of 275 semi-engorged females of\n                    <jats:italic>H. dromedarii<\/jats:italic>\n                    were collected from different slaughtered camels, Toukh city slaughterhouse then investigated by Polymerase Chain Reaction (PCR) to detect piroplasms (\n                    <jats:italic>Babesia<\/jats:italic>\n                    spp.,\n                    <jats:italic>Theileria<\/jats:italic>\n                    spp.) and Anaplasmataceae DNA targeting\n                    <jats:italic>18\u00a0S rRNA<\/jats:italic>\n                    and\n                    <jats:italic>16\u00a0S rRNA<\/jats:italic>\n                    genes, respectively followed by sequencing and phylogenetic analyses. Overall, piroplasms were detected in 38 ticks (13.8%),\n                    <jats:italic>Babesia<\/jats:italic>\n                    spp. was detected in 35 ticks (12.7%), while\n                    <jats:italic>Theileria<\/jats:italic>\n                    spp. was detected in one tick (0.4%). Anaplasmataceae was detected in 57 ticks (20.7%). Mixed infections of piroplasms and Anaplasmataceae were detected in 13 ticks (5%). Single infection either with piroplasms or Anaplasmataceae was detected in 25 (9%) and 44 (16%) ticks, respectively. The highest monthly rate of piroplasms was in April (spring) and Anaplasmataceae was in July (summer). Sequence analysis revealed that\n                    <jats:italic>Babesia bigemina<\/jats:italic>\n                    ,\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    spp. and\n                    <jats:italic>Anaplasma marginale<\/jats:italic>\n                    are the most dominant species in the examined tick samples. To the best of our knowledge, this study confirms the presence of\n                    <jats:italic>B. bigemina<\/jats:italic>\n                    ,\n                    <jats:italic>Wolbachia<\/jats:italic>\n                    spp. and\n                    <jats:italic>A. marginale<\/jats:italic>\n                    in\n                    <jats:italic>H. dromedarii<\/jats:italic>\n                    in Egypt by sequencing.\n                  <\/jats:p>","DOI":"10.1007\/s10493-024-00957-w","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T16:42:02Z","timestamp":1727282522000},"page":"849-868","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular screening of piroplasms and Anaplasmataceae agents in Hyalomma dromedarii ticks from camels over different seasons in Egypt"],"prefix":"10.1007","volume":"93","author":[{"given":"Bassma S. 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