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Conventional treatments such as exercise, pharmacological agents, and nutritional support offer limited efficacy, especially in older populations with reduced mobility or comorbidities. This study aimed to evaluate low-intensity pulsed ultrasound (LIPUS) as a novel, non-invasive therapeutic approach for age-related muscle atrophy. LIPUS was applied to the right hindlimbs of young (12-week), middle-aged (60-week), and aged (95-week) mice for 8\u00a0weeks. Muscle weights and mRNA expression levels were analyzed. In aged mice, LIPUS significantly increased gastrocnemius muscle mass and upregulated\n                    <jats:italic>Fndc5<\/jats:italic>\n                    and\n                    <jats:italic>Opa1<\/jats:italic>\n                    mRNA, genes associated with mitochondrial function and muscle regeneration. These findings suggest that LIPUS may serve as a safe, non-invasive intervention to counteract sarcopenia by promoting muscle growth and mitochondrial gene activation in aged skeletal muscle.\n                  <\/jats:p>","DOI":"10.1007\/s10522-025-10331-x","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T18:18:58Z","timestamp":1759515538000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of low-intensity pulsed ultrasound on muscle mass and Fndc5 mRNA expression in aged male\u00a0mice"],"prefix":"10.1007","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8012-5935","authenticated-orcid":false,"given":"Yoshitsugu","family":"Kojima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,3]]},"reference":[{"issue":"10","key":"10331_CR1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2024-009588","volume":"12","author":"S Acero-Bedoya","year":"2024","unstructured":"Acero-Bedoya S, Higgs EF, Martinez AC, Tonea R, Gajewski TF (2024) Dendritic cell-intrinsic PTPN22 negatively regulates antitumor immunity and impacts anti-PD-L1 efficacy. 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All animal experiments were approved by the Institutional Committee of Laboratory Animal Experimentation (Yokohama University of Pharmacy, Kanagawa, Japan).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}],"article-number":"187"}}