{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T04:03:29Z","timestamp":1770523409956,"version":"3.49.0"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T00:00:00Z","timestamp":1742428800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T00:00:00Z","timestamp":1742428800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biochem Genet"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1007\/s10528-025-11075-6","type":"journal-article","created":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T07:27:42Z","timestamp":1742542062000},"page":"1351-1368","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Screening Therapeutic Core Genes in Sepsis Using Network Pharmacology and Single-Cell RNA Sequencing"],"prefix":"10.1007","volume":"64","author":[{"given":"Guihong","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenglin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingchun","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaolan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,3,20]]},"reference":[{"issue":"1","key":"11075_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41596-020-00409-w","volume":"16","author":"TS Andrews","year":"2021","unstructured":"Andrews TS, Kiselev VY, McCarthy D, Hemberg M (2021) Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data. Nat Protoc 16(1):1\u20139","journal-title":"Nat Protoc"},{"issue":"14","key":"11075_CR2","doi-asserted-by":"publisher","first-page":"1457","DOI":"10.1001\/jama.2016.2762","volume":"315","author":"DC Angus","year":"2016","unstructured":"Angus DC, Opal S (2016) Immunosuppression and secondary infection in sepsis: part, not all, of the story. JAMA 315(14):1457\u20131459","journal-title":"JAMA"},{"issue":"2","key":"11075_CR3","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1093\/infdis\/jiad066","volume":"228","author":"J Bhardwaj","year":"2023","unstructured":"Bhardwaj J, Upadhye A, Gaskin EL, Doumbo S, Kayentao K, Ongoiba A, Traore B, Crompton PD, Tran TM (2023) Neither the African-centric S47 nor P72 variant of TP53 is associated with reduced risk of febrile malaria in a Malian Cohort Study. J Infect Dis 228(2):202\u2013211","journal-title":"J Infect Dis"},{"key":"11075_CR4","doi-asserted-by":"publisher","first-page":"317","DOI":"10.3389\/fgene.2019.00317","volume":"10","author":"G Chen","year":"2019","unstructured":"Chen G, Ning B, Shi T (2019) Single-cell RNA-seq technologies and related computational data analysis. Front Genet 10:317","journal-title":"Front Genet"},{"key":"11075_CR5","first-page":"696784","volume":"12","author":"Y Chen","year":"2021","unstructured":"Chen Y, Guo Q, Zhu Z, Zhang L, Dai X, Ying X et al (2021) Bioactive components and pharmacological action of Lonicera japonica Thunb. in the treatment of diabetes mellitus and its complications. Front Pharmacol 12:696784","journal-title":"Front Pharmacol"},{"issue":"1","key":"11075_CR6","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1186\/s12865-023-00588-7","volume":"24","author":"G Chen","year":"2023","unstructured":"Chen G, Zhang W, Wang C, Chen M, Hu Y, Wang Z (2023) Screening of four lysosome-related genes in sepsis based on RNA sequencing technology. BMC Immunol 24(1):50","journal-title":"BMC Immunol"},{"issue":"9","key":"11075_CR7","doi-asserted-by":"publisher","first-page":"1400","DOI":"10.1007\/s00134-018-5175-z","volume":"44","author":"CM Coopersmith","year":"2018","unstructured":"Coopersmith CM, De Backer D, Deutschman CS, Ferrer R, Lat I, Machado FR et al (2018) Surviving sepsis campaign: research priorities for sepsis and septic shock. Intensive Care Med 44(9):1400\u20131426","journal-title":"Intensive Care Med"},{"issue":"1","key":"11075_CR8","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1186\/s12879-024-09589-2","volume":"24","author":"Y Deng","year":"2024","unstructured":"Deng Y, Leng L, Wang C, Yang Q, Hu Y (2024) Analyzing the molecular mechanism of Scutellaria Radix in the treatment of sepsis using RNA sequencing. BMC Infect Dis 24(1):695","journal-title":"BMC Infect Dis"},{"key":"11075_CR9","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.i1585","volume":"353","author":"JE Gotts","year":"2016","unstructured":"Gotts JE, Matthay MA (2016) Sepsis: pathophysiology and clinical management. BMJ 353:i1585","journal-title":"BMJ"},{"issue":"12","key":"11075_CR10","doi-asserted-by":"publisher","first-page":"8060","DOI":"10.1002\/jcp.30494","volume":"236","author":"CK Han","year":"2021","unstructured":"Han CK, Lee WF, Hsu CJ, Huang YL, Lin CY, Tsai CH, Huang CC, Fong YC, Wu MH, Liu JF, Tang CH (2021) DPP4 reduces proinflammatory cytokine production in human rheumatoid arthritis synovial fibroblasts. J Cell Physiol 236(12):8060\u20138069","journal-title":"J Cell Physiol"},{"key":"11075_CR11","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.jep.2015.03.012","volume":"168","author":"ST Kao","year":"2015","unstructured":"Kao ST, Liu CJ, Yeh CC (2015) Protective and immunomodulatory effect of flos Lonicerae japonicae by augmenting IL-10 expression in a murine model of acute lung inflammation. J Ethnopharmacol 168:108\u2013115","journal-title":"J Ethnopharmacol"},{"key":"11075_CR12","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.addr.2018.12.005","volume":"141","author":"JL Karnell","year":"2019","unstructured":"Karnell JL, Rieder SA, Ettinger R, Kolbeck R (2019) Targeting the CD40-CD40L pathway in autoimmune diseases: humoral immunity and beyond. Adv Drug Deliv Rev 141:92\u2013103","journal-title":"Adv Drug Deliv Rev"},{"issue":"10","key":"11075_CR13","first-page":"18","volume":"102","author":"J Lee","year":"2019","unstructured":"Lee J, Levy MM (2019) Treatment of patients with severe sepsis and septic shock: current evidence-based practices. R I Med J 102(10):18\u201321","journal-title":"R I Med J"},{"issue":"1","key":"11075_CR14","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1038\/s41598-022-24878-z","volume":"13","author":"Y Li","year":"2023","unstructured":"Li Y, Wang C, Chen M (2023) Metabolomics-based study of potential biomarkers of sepsis. Sci Rep 13(1):585","journal-title":"Sci Rep"},{"issue":"1","key":"11075_CR15","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1001\/jama.2014.5804","volume":"312","author":"V Liu","year":"2014","unstructured":"Liu V, Escobar GJ, Greene JD, Soule J, Whippy A, Angus DC et al (2014) Hospital deaths in patients with sepsis from 2 independent cohorts. JAMA 312(1):90\u201392","journal-title":"JAMA"},{"key":"11075_CR16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.113364","volume":"264","author":"C Liu","year":"2021","unstructured":"Liu C, Yin Z, Feng T, Zhang M, Zhou Z, Zhou Y (2021) An integrated network pharmacology and RNA-Seq approach for exploring the preventive effect of Lonicerae japonicae flos on LPS-induced acute lung injury. J Ethnopharmacol 264:113364","journal-title":"J Ethnopharmacol"},{"issue":"3","key":"11075_CR17","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1097\/SHK.0000000000002072","volume":"59","author":"J Liu","year":"2023","unstructured":"Liu J, Li S, Xiong D, Shang W, Zhan T, Zhu X, He S, Wang Y, Zhang Q, Hu Y (2023) Screening of potential core genes in peripheral blood of adult patients with sepsis based on transcription regulation function. Shock 59(3):385\u2013392","journal-title":"Shock"},{"issue":"2","key":"11075_CR18","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1007\/s11684-020-0757-x","volume":"14","author":"L Ni","year":"2020","unstructured":"Ni L, Zhou L, Zhou M, Zhao J, Wang DW (2020) Combination of western medicine and Chinese traditional patent medicine in treating a family case of COVID-19 in Wuhan. Front Med 14(2):210\u2013214","journal-title":"Front Med"},{"issue":"12","key":"11075_CR19","doi-asserted-by":"publisher","first-page":"1889","DOI":"10.1097\/CCM.0000000000003342","volume":"46","author":"CJ Paoli","year":"2018","unstructured":"Paoli CJ, Reynolds MA, Sinha M, Gitlin M, Crouser E (2018) Epidemiology and costs of sepsis in the United States\u2014an analysis based on timing of diagnosis and severity level. Crit Care Med 46(12):1889\u20131897","journal-title":"Crit Care Med"},{"issue":"1","key":"11075_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1615\/CritRevImmunol.2022044927","volume":"42","author":"KA Preethi","year":"2022","unstructured":"Preethi KA, Selvakumar SC, Sekar D (2022) Diagnostic and therapeutic application of exosomal microRNAs inducing inflammation in type 2 diabetes mellitus. Crit Rev Immunol 42(1):1\u201311","journal-title":"Crit Rev Immunol"},{"key":"11075_CR21","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.toxlet.2019.02.005","volume":"306","author":"J Qian","year":"2019","unstructured":"Qian J, Chen X, Chen X, Sun C, Jiang Y, Qian Y, Zhang Y, Khan Z, Zhou J, Liang G, Zheng C (2019) Kaempferol reduces K63-linked polyubiquitination to inhibit nuclear factor-\u03baB and inflammatory responses in acute lung injury in mice. Toxicol Lett 306:53\u201360","journal-title":"Toxicol Lett"},{"issue":"5","key":"11075_CR22","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1056\/NEJMp1707170","volume":"377","author":"K Reinhart","year":"2017","unstructured":"Reinhart K, Daniels R, Kissoon N, Machado FR, Schachter RD, Finfer S (2017) Recognizing sepsis as a global health priority\u2014a WHO resolution. N Engl J Med 377(5):414\u2013417","journal-title":"N Engl J Med"},{"issue":"3","key":"11075_CR23","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1038\/s41591-020-0752-4","volume":"26","author":"M Reyes","year":"2020","unstructured":"Reyes M, Filbin MR, Bhattacharyya RP, Billman K, Eisenhaure T, Hung DT, Levy BD, Baron RM, Blainey PC, Goldberg MB et al (2020) An immune-cell signature of bacterial sepsis. Nat Med 26(3):333\u2013340","journal-title":"Nat Med"},{"issue":"10219","key":"11075_CR24","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/S0140-6736(19)32989-7","volume":"395","author":"KE Rudd","year":"2020","unstructured":"Rudd KE, Johnson SC, Agesa KM, Shackelford KA, Tsoi D, Kievlan DR et al (2020) Global, regional, and national sepsis incidence and mortality, 1990\u20132017: analysis for the Global Burden of Disease Study. The Lancet 395(10219):200\u2013211","journal-title":"The Lancet"},{"key":"11075_CR25","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/j.cyto.2018.03.042","volume":"110","author":"S Rungsung","year":"2018","unstructured":"Rungsung S, Singh TU, Rabha DJ, Kumar T, Cholenahalli Lingaraju M, Parida S, Paul A, Sahoo M, Kumar D (2018) Luteolin attenuates acute lung injury in experimental mouse model of sepsis. Cytokine 110:333\u2013343","journal-title":"Cytokine"},{"issue":"1","key":"11075_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jep.2011.08.016","volume":"138","author":"X Shang","year":"2011","unstructured":"Shang X, Pan H, Li M, Miao X, Ding H (2011) Lonicera japonica Thunb.: ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine. J Ethnopharmacol 138(1):1\u201321","journal-title":"J Ethnopharmacol"},{"issue":"8","key":"11075_CR27","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1001\/jama.2016.0287","volume":"315","author":"M Singer","year":"2016","unstructured":"Singer M, Deutschman CS, Seymour CW, Shankar-Hari M, Annane D, Bauer M et al (2016) The third international consensus definitions for sepsis and septic shock (sepsis-3). JAMA 315(8):801\u2013810","journal-title":"JAMA"},{"issue":"1","key":"11075_CR28","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1515\/bmc-2021-0010","volume":"12","author":"A Singh","year":"2021","unstructured":"Singh A, Anang V, Verma C, Saraswati SSK, Rana AK, Bandyopadhyay U, Chadha A, Natarajan K (2021) Bcl2 negatively regulates protective immune responses during mycobacterial infection. Biomol Concepts 12(1):94\u2013109","journal-title":"Biomol Concepts"},{"key":"11075_CR29","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.113502","volume":"267","author":"X Su","year":"2021","unstructured":"Su X, Zhu ZH, Zhang L et al (2021) Anti-inflammatory property and functional substances of Lonicerae japonicae caulis. J Ethnopharmacol 267:113502","journal-title":"J Ethnopharmacol"},{"issue":"2\u20133","key":"11075_CR30","first-page":"121","volume":"80","author":"TF Tzeng","year":"2014","unstructured":"Tzeng TF, Liou SS, Chang CJ et al (2014) The ethanol extract of Lonicera japonica (Japanese honeysuckle) attenuates diabetic nephropathy by inhibiting p-38 MAPK activity in streptozotocin-induced diabetic rats. Planta Med 80(2\u20133):121\u2013129","journal-title":"Planta Med"},{"issue":"7","key":"11075_CR31","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1038\/nri.2017.36","volume":"17","author":"T van der Poll","year":"2017","unstructured":"van der Poll T, van de Veerdonk FL, Scicluna BP, Netea MG (2017) The immunopathology of sepsis and potential therapeutic targets. Nat Rev Immunol 17(7):407\u2013420","journal-title":"Nat Rev Immunol"},{"issue":"13","key":"11075_CR32","doi-asserted-by":"publisher","first-page":"1560","DOI":"10.2174\/1871520619666190705150214","volume":"19","author":"M Vinayak","year":"2019","unstructured":"Vinayak M, Maurya AK (2019) Quercetin loaded nanoparticles in targeting cancer: recent development. Anticancer Agents Med Chem 19(13):1560\u20131576","journal-title":"Anticancer Agents Med Chem"},{"issue":"3","key":"11075_CR33","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1007\/s10753-018-0761-3","volume":"41","author":"XF Wang","year":"2018","unstructured":"Wang XF, Song SD, Li YJ, Hu ZQ, Zhang ZW, Yan CG, Li ZG, Tang HF (2018) Protective effect of quercetin in LPS-induced murine acute lung injury mediated by cAMP-Epac pathway. Inflammation 41(3):1093\u20131103","journal-title":"Inflammation"},{"key":"11075_CR34","doi-asserted-by":"publisher","first-page":"5575","DOI":"10.2147\/IDR.S380137","volume":"15","author":"C Wang","year":"2022","unstructured":"Wang C, Li Y, Li S, Chen M, Hu Y (2022a) Proteomics combined with RNA sequencing to screen biomarkers of sepsis. Infect Drug Resist 15:5575\u20135587","journal-title":"Infect Drug Resist"},{"issue":"15","key":"11075_CR35","doi-asserted-by":"publisher","first-page":"7667","DOI":"10.2147\/IDR.S393654","volume":"23","author":"C Wang","year":"2022","unstructured":"Wang C, Li S, Shen Y, Li Y, Chen M, Wang Y, Lan Y, Hu Y (2022b) Mechanisms of Panax Ginseng on treating sepsis by RNA-seq technology. Infect Drug Resist 23(15):7667\u20137678","journal-title":"Infect Drug Resist"},{"issue":"3","key":"11075_CR36","first-page":"936","volume":"24","author":"W Wei","year":"2019","unstructured":"Wei W, He J, Ruan H, Wang Y (2019) In vitro and in vivo cytotoxic effects of chrysoeriol in human lung carcinoma are facilitated through activation of autophagy, sub-G1\/G0 cell cycle arrest, cell migration and invasion inhibition and modulation of MAPK\/ERK signalling pathway. J BUON 24(3):936\u2013942","journal-title":"J BUON"},{"issue":"8","key":"11075_CR37","doi-asserted-by":"publisher","first-page":"762","DOI":"10.1080\/10286020.2019.1642329","volume":"22","author":"S Wu","year":"2020","unstructured":"Wu S, Wang HQ, Guo TT et al (2020) Luteolin inhibits CVB3 replication through inhibiting inflammation. J Asian Nat Prod Res 22(8):762\u2013773","journal-title":"J Asian Nat Prod Res"},{"key":"11075_CR38","doi-asserted-by":"publisher","first-page":"113364","DOI":"10.1016\/j.jep.2020.113364","volume":"264","author":"Z Yin","year":"2021","unstructured":"Yin Z, Feng T, Zhang M, Zhou Z, Zhou Y (2021) An integrated network pharmacology and RNA-Seq approach for exploring the preventive effect of Lonicerae japonicae flos on LPS-induced acute lung injury. J Ethnopharmacol 264:113364","journal-title":"J Ethnopharmacol"}],"container-title":["Biochemical Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10528-025-11075-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10528-025-11075-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10528-025-11075-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T05:18:23Z","timestamp":1770441503000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10528-025-11075-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,20]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["11075"],"URL":"https:\/\/doi.org\/10.1007\/s10528-025-11075-6","relation":{},"ISSN":["0006-2928","1573-4927"],"issn-type":[{"value":"0006-2928","type":"print"},{"value":"1573-4927","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,20]]},"assertion":[{"value":"30 July 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 February 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 March 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The study was conducted in accordance with the Declaration of Helsinki and approved by the Ethics Committee of the Affiliated Hospital of Southwest Medical University (Ethics Number: ky2018029), with the clinical trial Registration Number: ChiCTR1900021261 (\n                      \n                      , Registration Date: 2019-02-04).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"All subjects and their legal representatives signed informed consent.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}}]}}