{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:58:03Z","timestamp":1791280683005,"version":"4.1.0"},"reference-count":15,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2010,11,20]],"date-time":"2010-11-20T00:00:00Z","timestamp":1290211200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnol Lett"],"published-print":{"date-parts":[[2011,3]]},"DOI":"10.1007\/s10529-010-0474-7","type":"journal-article","created":{"date-parts":[[2010,11,19]],"date-time":"2010-11-19T10:26:29Z","timestamp":1290162389000},"page":"617-621","source":"Crossref","is-referenced-by-count":12,"title":["Stabilization of a supplemental digestive enzyme by post-translational engineering using chemically-activated polyethylene glycol"],"prefix":"10.1007","volume":"33","author":[{"given":"Kevin M.","family":"Turner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianfranco","family":"Pasut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco M.","family":"Veronese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Boyce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Walsh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2010,11,20]]},"reference":[{"key":"474_CR1","doi-asserted-by":"crossref","DOI":"10.1079\/9780851993935.0000","volume-title":"Enzymes in farm animal nutrition","author":"M Bedford","year":"2001","unstructured":"Bedford M, Partridge G (2001) Enzymes in farm animal nutrition. CABI publishing, Oxford, UK"},{"key":"474_CR2","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/0003-2697(92)90460-O","volume":"200","author":"MM Kurfurst","year":"1992","unstructured":"Kurfurst MM (1992) Detection and molecular weight determination of polyethylene glycol-modified hirudin by staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem 200:244\u2013248","journal-title":"Anal Biochem"},{"key":"474_CR3","first-page":"261","volume-title":"Directory of therapeutic enzymes","author":"S O\u2019Connell","year":"2006","unstructured":"O\u2019Connell S (2006) Additional therapeutic enzymes. In: McGrath B, Walsh G (eds) Directory of therapeutic enzymes. Tailor and Francis, Florida, pp 261\u2013290"},{"key":"474_CR4","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1385\/ABAB:134:2:179","volume":"134","author":"S O\u2019Connell","year":"2006","unstructured":"O\u2019Connell S, Walsh G (2006) Physicochemical characteristics of commercial lactases relevant to their application in the alleviation of lactose intolerance. App Biochem Biotechnol 134:179\u2013191","journal-title":"App Biochem Biotechnol"},{"key":"474_CR5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12010-007-9206-4","volume":"141","author":"S O\u2019Connell","year":"2007","unstructured":"O\u2019Connell S, Walsh G (2007) Purification and properties of a beta-galactosidase with potential application as a digestive supplement. App Biochem Biotechnol 141:1\u201313","journal-title":"App Biochem Biotechnol"},{"key":"474_CR6","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s12010-007-8098-7","volume":"149","author":"S O\u2019Connell","year":"2008","unstructured":"O\u2019Connell S, Walsh G (2008) Application relevant studies of fungal beta-galactosidases with potential application in the alleviation of lactose intolerance. App Biochem Biotechnol 149:129\u2013138","journal-title":"App Biochem Biotechnol"},{"key":"474_CR7","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.progpolymsci.2007.05.008","volume":"32","author":"G Pasut","year":"2007","unstructured":"Pasut G, Veronese FM (2007) Polymer-drug conjugation, recent achievements and general strategies. Prog Polym Sci 32:933\u2013961","journal-title":"Prog Polym Sci"},{"key":"474_CR8","doi-asserted-by":"crossref","first-page":"15135","DOI":"10.1021\/bi026238c","volume":"41","author":"CL Ryttersgaard","year":"2002","unstructured":"Ryttersgaard CL, Lo Leggio P, Coutinho M, Henrissat B, Larsen S (2002) Aspergillus aculeatus beta-1,4-galactanase: substrate recognition and relations to other glycoside hydrolases in clan GH-A. Biochemistry 41:15135\u201315143","journal-title":"Biochemistry"},{"key":"474_CR9","first-page":"1845","volume":"65","author":"DL Swagerty","year":"2002","unstructured":"Swagerty DL, Walling AD Jr, Klein RM (2002) Lactose intolerance. Am Fam Physician 65:1845\u20131850","journal-title":"Am Fam Physician"},{"key":"474_CR10","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/S0142-9612(00)00193-9","volume":"22","author":"FM Veronese","year":"2001","unstructured":"Veronese FM (2001) Peptide and protein PEGylation: a review of problems and solutions. Biomaterials 22:405\u2013417","journal-title":"Biomaterials"},{"issue":"3","key":"474_CR11","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1177\/088391159701200303","volume":"12","author":"FM Veronese","year":"1997","unstructured":"Veronese FM, Caliceti P, Schiavon O (1997) Branched and linear poly(ethylene glycol): influence of the polymer structure on enzymological, pharmacokinetic, and immunological properties of protein conjugates. J Bioact Compat Pol 12(3):196\u2013207","journal-title":"J Bioact Compat Pol"},{"issue":"1","key":"474_CR12","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1021\/bc000061m","volume":"12","author":"FM Veronese","year":"2001","unstructured":"Veronese FM, Sacca B, de Laureto PP, Sergi M, Caliceti P, Schiavon O, Orsolini P (2001) New PEGs for peptide and protein modification, suitable for identification of the PEGylation site. Bioconjugate Chem 12(1):62\u201370","journal-title":"Bioconjugate Chem"},{"key":"474_CR13","doi-asserted-by":"crossref","first-page":"5535","DOI":"10.1021\/jf900369e","volume":"57","author":"H Wang","year":"2009","unstructured":"Wang H, Lou H, Bai Y, Wang Y, Yang P, Shi P, Zhang W, Fan Y, Yao B (2009) An acidophilic \u03b2-Galactosidase from Bispora sp. MEY-1 with high lactose hydrolytic activity under simulated gastric conditions. J Agric Food Chem 57:5535\u20135541","journal-title":"J Agric Food Chem"},{"issue":"2","key":"474_CR14","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1007\/BF03189363","volume":"23","author":"K Xenos","year":"1998","unstructured":"Xenos K, Kyroundis S, Anagnostidis A, Papastathopoulos P (1998) Treatment of lactose intolerance with exogenous beta-D-galactosidase in pellet form. Eur J Drug Metab 23(2):350\u2013355","journal-title":"Eur J Drug Metab"},{"issue":"16","key":"474_CR15","doi-asserted-by":"crossref","first-page":"2801","DOI":"10.1002\/elps.200600807","volume":"28","author":"C Zheng","year":"2007","unstructured":"Zheng C, Ma G, Su Z (2007) Native PAGE eliminates the problem of PEG-SDS interaction in SDS-PAGE and provides an alternative to HPLC in characterization of protein PEGylation. Electrophoresis 28(16):2801\u20132807","journal-title":"Electrophoresis"}],"container-title":["Biotechnology Letters"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10529-010-0474-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10529-010-0474-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10529-010-0474-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,13]],"date-time":"2021-11-13T19:53:32Z","timestamp":1636833212000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10529-010-0474-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,20]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,3]]}},"alternative-id":["474"],"URL":"https:\/\/doi.org\/10.1007\/s10529-010-0474-7","relation":{},"ISSN":["0141-5492","1573-6776"],"issn-type":[{"value":"0141-5492","type":"print"},{"value":"1573-6776","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11,20]]}}}