{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T21:44:44Z","timestamp":1749678284012,"version":"3.37.3"},"reference-count":15,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T00:00:00Z","timestamp":1440460800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81302694"],"award-info":[{"award-number":["81302694"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81202967","81274150"],"award-info":[{"award-number":["81202967","81274150"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Shanghai Municipal Natural Science Foundation","doi-asserted-by":"crossref","award":["13ZR1448900"],"award-info":[{"award-number":["13ZR1448900"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["12ZR1415600"],"award-info":[{"award-number":["12ZR1415600"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biotechnol Lett"],"published-print":{"date-parts":[[2015,12]]},"DOI":"10.1007\/s10529-015-1936-8","type":"journal-article","created":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T04:52:32Z","timestamp":1440391952000},"page":"2461-2466","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["In vitro refolding and functional analysis of polyhistidine-tagged Buthus martensii Karsch antitumor-analgesic peptide produced in Escherichia coli"],"prefix":"10.1007","volume":"37","author":[{"given":"Qingxin","family":"Cao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wuguang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueting","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunping","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaijiang","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiege","family":"Huo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changquan","family":"Ling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,8,25]]},"reference":[{"key":"1936_CR1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1038\/6192","volume":"17","author":"MM Altamirano","year":"1999","unstructured":"Altamirano MM, Garcia C, Possani LD, Fersht AR (1999) Oxidative refolding chromatography: folding of the scorpion toxin Cn5. Nat Biotechnol 17:187\u2013191","journal-title":"Nat Biotechnol"},{"key":"1936_CR2","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1007\/s12033-010-9363-5","volume":"48","author":"KN Balduino","year":"2011","unstructured":"Balduino KN, Spencer PJ, Malavasi NV, Chura-Chambi RM, Lemke LS, Morganti L (2011) Refolding by high pressure of a toxin containing seven disulfide bonds: bothropstoxin-1 from Bothrops jararacussu. Mol Biotechnol 48:228\u2013234","journal-title":"Mol Biotechnol"},{"key":"1936_CR3","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1038\/nbt1029","volume":"22","author":"F Baneyx","year":"2004","unstructured":"Baneyx F, Mujacic M (2004) Recombinant protein folding and misfolding in Escherichia coli. Nat Biotechnol 22:1399\u20131408","journal-title":"Nat Biotechnol"},{"key":"1936_CR4","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1002\/btpr.433","volume":"26","author":"P Cao","year":"2010","unstructured":"Cao P et al (2010) Expression and purification of an antitumor-analgesic peptide from the venom of Mesobuthus martensii Karsch by small ubiquitin-related modifier fusion in Escherichia coli. Biotechnol Prog 26:1240\u20131244","journal-title":"Biotechnol Prog"},{"key":"1936_CR5","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.bbagen.2007.04.006","volume":"1770","author":"G Estrada","year":"2007","unstructured":"Estrada G et al (2007) Four disulfide-bridged scorpion beta neurotoxin CssII: heterologous expression and proper folding in vitro. Biochim Biophys Acta 1770:1161\u20131168","journal-title":"Biochim Biophys Acta"},{"key":"1936_CR6","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/S0041-0101(02)00142-3","volume":"40","author":"C Goudet","year":"2002","unstructured":"Goudet C, Chi CW, Tytgat J (2002) An overview of toxins and genes from the venom of the Asian scorpion Buthus martensi Karsch. Toxicon 40:1239\u20131258","journal-title":"Toxicon"},{"key":"1936_CR7","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S2095-4964(14)60038-8","volume":"12","author":"CQ Ling","year":"2014","unstructured":"Ling CQ, Yue XQ, Ling C (2014) Three advantages of using traditional Chinese medicine to prevent and treat tumor. J Integrat Med 12:331\u2013335","journal-title":"J Integrat Med"},{"key":"1936_CR8","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S1046-5928(02)00609-5","volume":"27","author":"YF Liu","year":"2003","unstructured":"Liu YF, Ma RL, Wang SL, Duan ZY, Zhang JH, Wu LJ, Wu CF (2003) Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli. Prot Expr Purif 27:253\u2013258","journal-title":"Prot Expr Purif"},{"key":"1936_CR9","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1134\/S0006297909100101","volume":"74","author":"EN Lyukmanova","year":"2009","unstructured":"Lyukmanova EN et al (2009) Bacterial production and refolding from inclusion bodies of a \u201cWeak\u201d toxin, a disulfide rich protein. Biochem (Moscow) 74:1142\u20131149","journal-title":"Biochem (Moscow)"},{"key":"1936_CR10","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.bbrc.2010.02.179","volume":"394","author":"R Ma","year":"2010","unstructured":"Ma R et al (2010) Location of the analgesic domain in Scorpion toxin BmK AGAP by mutagenesis of disulfide bridges. Biochem Biophys Res Commun 394:330\u2013334","journal-title":"Biochem Biophys Res Commun"},{"key":"1936_CR11","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.1046\/j.1432-1033.2003.03743.x","volume":"270","author":"S M\u2019Barek","year":"2003","unstructured":"M\u2019Barek S et al (2003) Synthesis and characterization of Pi4, a scorpion toxin from Pandinus imperator that acts on K+ channels. Eur J Biochem 270:3583\u20133592","journal-title":"Eur J Biochem"},{"key":"1936_CR12","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1016\/j.toxicon.2005.09.006","volume":"46","author":"RC Rodriguez de la Vega","year":"2005","unstructured":"Rodriguez de la Vega RC, Possani LD (2005) Overview of scorpion toxins specific for Na+ channels and related peptides: biodiversity, structure-function relationships and evolution. Toxicon 46:831\u2013844","journal-title":"Toxicon"},{"key":"1936_CR13","first-page":"6","volume":"2","author":"JH Shao","year":"2007","unstructured":"Shao JH, Zhang R, Ge X, Yang B, Zhang JH (2007) Analgesic peptides in Buthus martensii Karsch: a traditional chinese animal medicine. Asian J Trad Med 2:6","journal-title":"Asian J Trad Med"},{"key":"1936_CR14","doi-asserted-by":"crossref","first-page":"499","DOI":"10.5505\/tjb.2013.84429","volume":"38","author":"J Shao","year":"2013","unstructured":"Shao J, Zhao C, Hui C, Zhao R, Ruan X (2013) Molecular cloning and functional identification of analgesic peptides from Buthus martensii Karsch. Turk J Biochem 38:499\u2013505","journal-title":"Turk J Biochem"},{"key":"1936_CR15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1124\/mol.110.070540","volume":"80","author":"MJ Windley","year":"2011","unstructured":"Windley MJ, Escoubas P, Valenzuela SM, Nicholson GM (2011) A novel family of insect-selective peptide neurotoxins targeting insect large-conductance calcium-activated K+ channels isolated from the venom of the theraphosid spider Eucratoscelus constrictus. Mol Pharmacol 80:1\u201313","journal-title":"Mol Pharmacol"}],"container-title":["Biotechnology Letters"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10529-015-1936-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10529-015-1936-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10529-015-1936-8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,29]],"date-time":"2019-05-29T16:05:17Z","timestamp":1559145917000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10529-015-1936-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8,25]]},"references-count":15,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["1936"],"URL":"https:\/\/doi.org\/10.1007\/s10529-015-1936-8","relation":{},"ISSN":["0141-5492","1573-6776"],"issn-type":[{"type":"print","value":"0141-5492"},{"type":"electronic","value":"1573-6776"}],"subject":[],"published":{"date-parts":[[2015,8,25]]}}}