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In this study, we evaluated the preservation efficacy of discarded human donor corneas using a Thermo-reversible gelation polymer (TGP) added to these two media.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Thirteen human corneal buttons collected from deceased donors, which were otherwise discarded due to low endothelial cell density (ECD) were used. They were stored in four groups: MK medium, MK medium with TGP, Optisol-GS and Optisol-GS with TGP at 4\u00a0\u00b0C for 96\u00a0h. Slit lamp examination and specular microscopy were performed. Corneal limbal tissues from these corneas were then cultured using explant methodology one with and the other without TGP scaffold, for 21\u00a0days.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>MK\u2009+\u2009TGP and Optisol-GS\u2009+\u2009TGP preserved corneas better than without TGP, which was observed by maintenance of ECD which was significantly higher in Optisol-GS\u2009+\u2009TGP than MK\u2009+\u2009TGP (p-value\u2009=\u20090.000478) and corneal thickness remaining the same for 96\u00a0h. Viable corneal epithelial cells could be grown from the corneas stored only in MK\u2009+\u2009TGP and Optisol-GS\u2009+\u2009TGP. During culture, the TGP scaffold helped maintain the native epithelial phenotype and progenitor\/stem cell growth was confirmed by RT-PCR characterization.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>TGP reconstituted with MK and Optisol\u2014GS media yields better preservation of human corneal buttons in terms of relatively higher ECD maintenance and better in vitro culture outcome of corneal limbal tissue. This method has the potential to become a standard donor corneal transportation-preservation methodology and it can also be extended to other tissue or organ transportation upon further validation.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s10529-021-03116-y","type":"journal-article","created":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T22:57:59Z","timestamp":1616713079000},"page":"1241-1251","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells"],"prefix":"10.1007","volume":"43","author":[{"given":"Bhuvaneshwari","family":"Namitha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Munusamy Rajendran","family":"Chitra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathevan","family":"Bhavya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Periasamy","family":"Parikumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shojiro","family":"Katoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Yoshioka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Iwasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajappa","family":"Senthilkumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathaiyan","family":"Rajmohan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramalingam","family":"Karthick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Senthilkumar","family":"Preethy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2646-2687","authenticated-orcid":false,"given":"Samuel J. 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Dr. Yoshioka is an employee of Mebiol Inc and an applicant to several patents on TGP and its applications. Dr. Abraham is a shareholder in GN Corporation Co. Ltd., Japan, an applicant to several patents on biomaterials and cell culture methodologies, some of them described in this manuscript.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The institutional ethics committee of Regional Institute of Ophthalmology, Egmore, Chennai, Tamil Nadu, India approved the study.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}