{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:14:38Z","timestamp":1789535678805,"version":"build-2803163510"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"5-6","license":[{"start":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T00:00:00Z","timestamp":1681862400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T00:00:00Z","timestamp":1681862400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biotechnol Lett"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1007\/s10529-023-03371-1","type":"journal-article","created":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T05:02:01Z","timestamp":1681880521000},"page":"703-717","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Synthesis of plant-based, self-adjuvanted, dual antigen specific to Mycobacterium tuberculosis as a novel tuberculosis subunit vaccine that elicits immunogenicity in rabbit"],"prefix":"10.1007","volume":"45","author":[{"given":"Jyoti","family":"Yadav","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supriya","family":"Phogat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darshna","family":"Chaudhary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjana","family":"Jaiwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3143-4103","authenticated-orcid":false,"given":"Pawan K.","family":"Jaiwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,4,19]]},"reference":[{"key":"3371_CR1","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford MM (1976) A rapid and sensitive method for the quantisation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248\u2013254","journal-title":"Anal Biochem"},{"key":"3371_CR2","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1186\/s12879-021-06022-w","volume":"21","author":"XJ Cao","year":"2021","unstructured":"Cao XJ, Li YP, Wang JY et al (2021) MPT64 assays for the rapid detection of Mycobacterium tuberculosis. BMC Infect Dis 21:336. https:\/\/doi.org\/10.1186\/s12879-021-06022-w","journal-title":"BMC Infect Dis"},{"key":"3371_CR3","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1007\/BF00232293","volume":"9","author":"PP Chee","year":"1990","unstructured":"Chee PP (1990) Transformation of Cucumis sativus tissue by Agrobacterium tumefaciens and the regeneration of transformed plants. Plant Cell Rep 9:245\u2013248","journal-title":"Plant Cell Rep"},{"key":"3371_CR4","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/274346","author":"DM Floss","year":"2010","unstructured":"Floss DM, Mockey M, Zanello G, Brosson D, Diogon M, Frutos R, Bruel T, Rodrigues V, Garzon E, Chevaleyre C (2010) Expression and immunogenicity of the mycobacterial Ag85B\/ESAT-6 antigens produced in transgenic plants by elastin-like peptide fusion strategy. Biomed Res Int. https:\/\/doi.org\/10.1155\/2010\/274346","journal-title":"Biomed Res Int"},{"key":"3371_CR5","unstructured":"Ghebreyesus TA (2020) WHO Director-General\u2019s opening remarks at the media briefing on COVID-19 - 11 March 2020. Geneva: World Health Organization"},{"key":"3371_CR6","unstructured":"Global Tuberculosis Report (2021) WHO, https:\/\/ddg-pharmfac.net\/AllergenFP\/feedback.py"},{"key":"3371_CR7","doi-asserted-by":"publisher","first-page":"908","DOI":"10.1128\/JCM.40.3.908-912.2002","volume":"40","author":"N Hasegawa","year":"2002","unstructured":"Hasegawa N, Miura T, Ishii K, Yamaguchi K, Lindner TH, Merritt S, Matthews JD, Siddiqi SH (2002) New simple and rapid test for culture confirmation of Mycobacterium tuberculosis complex: a multicenter study. J Clin Microbiol 40:908\u2013912. https:\/\/doi.org\/10.1128\/JCM.40.3.908-912.2002","journal-title":"J Clin Microbiol"},{"key":"3371_CR8","doi-asserted-by":"publisher","first-page":"1575","DOI":"10.1016\/j.molp.2017.09.005","volume":"10","author":"B Hu","year":"2017","unstructured":"Hu B, Li D, Liu X, Qi J, Gao D, Zhao S, Huang S, Sun J, Yang L (2017) Engineering non-transgenic gynoecious cucumber using an improved transformation protocol and optimized CRISPR\/Cas9 system. Mol Plant 10:1575\u20131578. https:\/\/doi.org\/10.1016\/j.molp.2017.09.005","journal-title":"Mol Plant"},{"key":"3371_CR9","doi-asserted-by":"publisher","first-page":"3095","DOI":"10.4049\/jimmunol.1801357","volume":"205","author":"V Jha","year":"2020","unstructured":"Jha V, Pal R, Kumar D, Mukhopadhyay S (2020) ESAT-6 protein of\u00a0Mycobacterium tuberculosis\u00a0increases holotransferrin-mediated iron uptake in macrophages by down regulating surface hemochromatosis protein HFE. J Immunol (Baltimore, Md.:1950) 205:3095\u20133106. https:\/\/doi.org\/10.4049\/jimmunol.1801357","journal-title":"J Immunol (Baltimore, Md.:1950)"},{"key":"3371_CR10","doi-asserted-by":"publisher","first-page":"2721","DOI":"10.1080\/21505594.2021.1990660","volume":"12","author":"H Joshi","year":"2021","unstructured":"Joshi H, Kandari D, Bhatnagar R (2021) Insights into the molecular determinants involved in Mycobacterium tuberculosis persistence and their therapeutic implications. Virulence 12:2721\u20132749. https:\/\/doi.org\/10.1080\/21505594.2021.1990660","journal-title":"Virulence"},{"key":"3371_CR11","doi-asserted-by":"publisher","first-page":"750124","DOI":"10.3389\/fmicb.2021.750124","volume":"12","author":"SHE Kaufmann","year":"2021","unstructured":"Kaufmann SHE (2021) Vaccine development against tuberculosis over the last 140 years: Failure as part of success. Front Microbiol 12:750124. https:\/\/doi.org\/10.3389\/fmicb.2021.750124","journal-title":"Front Microbiol"},{"key":"3371_CR12","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1186\/1756-0500-4-79","volume":"4","author":"VG Kumar","year":"2011","unstructured":"Kumar VG, Urs TA, Ranganath RR (2011) MPT 64 antigen detection for rapid confirmation of M. tuberculosis isolates. BMC Res Notes 4:79. https:\/\/doi.org\/10.1186\/1756-0500-4-79","journal-title":"BMC Res Notes"},{"key":"3371_CR13","doi-asserted-by":"publisher","first-page":"782","DOI":"10.1016\/j.addr.2012.10.005","volume":"65","author":"KC Kwon","year":"2013","unstructured":"Kwon KC, Verma D, Singh ND, Herzog R, Daniell H (2013) Oral delivery of human biopharmaceuticals, autoantigens and vaccine antigens bioencapsulated in plant cells. Adv Drug Deliv Rev 65:782\u2013799. https:\/\/doi.org\/10.1016\/j.addr.2012.10.005","journal-title":"Adv Drug Deliv Rev"},{"key":"3371_CR14","doi-asserted-by":"publisher","first-page":"e54708","DOI":"10.1371\/journal.pone.0054708","volume":"8","author":"PS Lakshmi","year":"2013","unstructured":"Lakshmi PS, Verma D, Yang X, Lloyd B, Daniell H (2013) Low cost tuberculosis vaccine antigens in capsules: expression in chloroplasts, bio-encapsulation, stability and functional evaluation in vitro. PLoS One 8:e54708. https:\/\/doi.org\/10.1371\/journal.pone.0054708","journal-title":"PLoS One"},{"key":"3371_CR15","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1086\/345862","volume":"187","author":"KN Lewis","year":"2003","unstructured":"Lewis KN, Liao R, Guinn KM, Hickey MJ, Smith S, Behr MA, Sherman DR (2003) Deletion of RD1 from Mycobacterium tuberculosis mimics Bacilli Calmette-Gu\u00e9rin attenuation. J Infect Dis 187:117\u2013123. https:\/\/doi.org\/10.1086\/345862","journal-title":"J Infect Dis"},{"key":"3371_CR16","first-page":"835","volume":"123","author":"SS Mane","year":"2022","unstructured":"Mane SS (2022) Tuberculosis: the silent pandemic. Curr Sci 123:835\u2013836","journal-title":"Curr Sci"},{"key":"3371_CR17","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1186\/s13568-018-0708-y","volume":"8","author":"DG M\u00f3dolo","year":"2018","unstructured":"M\u00f3dolo DG, Horn CS, Soares JSM, Yunes JA, Lima LM, de Sousa SM, Menossi M (2018) Transgenic Nicotiana tabacum seeds expressing the Mycobacterium tuberculosis alanine and proline-rich antigen. AMB Express 8:178. https:\/\/doi.org\/10.1186\/s13568-018-0708-y","journal-title":"AMB Express"},{"key":"3371_CR18","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","volume":"15","author":"T Murashige","year":"1962","unstructured":"Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiol 15:473\u2013497","journal-title":"Plant Physiol"},{"key":"3371_CR19","doi-asserted-by":"publisher","first-page":"141","DOI":"10.5511\/plantbiotechnology.20.0225a","volume":"37","author":"Y Nanasato","year":"2020","unstructured":"Nanasato Y, Tabei Y (2020) A method of transformation and current progress in transgenic research on cucumbers and Cucurbita species. Plant Biotechnol 37:141\u2013146. https:\/\/doi.org\/10.5511\/plantbiotechnology.20.0225a","journal-title":"Plant Biotechnol"},{"key":"3371_CR20","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1007\/s11816-012-0260-1","volume":"7","author":"Y Nanasato","year":"2013","unstructured":"Nanasato Y, Konagaya K, Okuzaki A, Tsuda M, Tabei Y (2013) Improvement of Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.) by combination of vacuum infiltration and co-cultivation on filter paper wicks. Plant Biotechnol Rep 7:267\u2013276. https:\/\/doi.org\/10.1007\/s11816-012-0260-1","journal-title":"Plant Biotechnol Rep"},{"key":"3371_CR21","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz034","author":"E Nemes","year":"2019","unstructured":"Nemes E, Abrahams D, Scriba TJ, Ratangee F, Keyser A, Makhethe L et al (2019) Diagnostic accuracy of early secretory antigenic target-6\u2013free interferon-gamma release assay compared to quantiFERON-TB gold in-tube. Clin Infect Dis. https:\/\/doi.org\/10.1093\/cid\/ciz034","journal-title":"Clin Infect Dis"},{"key":"3371_CR22","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/417565","author":"NV Permyakova","year":"2015","unstructured":"Permyakova NV, Zagorskaya AA, Belavin PA, Uvarova EA, Nosareva OV, Nesterov AE (2015) Transgenic carrot expressing fusion protein comprising M. tuberculosis antigens induces immune response in mice. Biomed Res Int. https:\/\/doi.org\/10.1155\/2015\/417565","journal-title":"Biomed Res Int"},{"key":"3371_CR23","first-page":"431","volume":"40","author":"PA Rajagoplan","year":"2005","unstructured":"Rajagoplan PA, Perl-Treves R (2005) Improved cucumber transformation by a modified explant dissection and selection protocol. Hortic Sci 40:431\u2013435","journal-title":"Hortic Sci"},{"key":"3371_CR24","doi-asserted-by":"publisher","first-page":"327","DOI":"10.3389\/fcimb.2018.00327","volume":"8","author":"A Refai","year":"2018","unstructured":"Refai A, Gritli S, Barbouche MR, Essafi M (2018) Mycobacterium tuberculosis virulent factor ESAT-6 drives macrophage differentiation toward the pro-inflammatory M1 phenotype and subsequently switches it to the anti-inflammatory M2 phenotype. Front Cell Infect Microbiol 8:327. https:\/\/doi.org\/10.3389\/fcimb.2018.00327","journal-title":"Front Cell Infect Microbiol"},{"key":"3371_CR25","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1007\/978-94-009-0951-9_6","volume-title":"Extraction of DNA from plant tissues Plant Mol Biol manual","author":"SO Rogers","year":"1989","unstructured":"Rogers SO, Bendich AJ (1989) Extraction of DNA from plant tissues Plant Mol Biol manual. Kluwer Academic Publishers Springer, Dordrecht, pp 73\u201383"},{"key":"3371_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jbiotec.2019.08.016","volume":"305","author":"K Saba","year":"2019","unstructured":"Saba K, Gottschamel J, Younus I, Syed T, Gull K, Lossl AG, Mirza B, Waheed MT (2019a) Chloroplast-based inducible expression of ESAT-6 antigen for development of a plant-based vaccine against tuberculosis. J Biotechnol 305:1\u201310. https:\/\/doi.org\/10.1016\/j.jbiotec.2019.08.016","journal-title":"J Biotechnol"},{"key":"3371_CR27","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1002\/bab.1867","volume":"67","author":"K Saba","year":"2019","unstructured":"Saba K, Sameeullah M, Asghar A, Gottschamel J, Latif S, Lossl AG, Mirza B, Mirza O, Waheed MT (2019b) Expression of ESAT-6 antigen from Mycobacterium tuberculosis in broccoli: an edible plant. Biotech Appl Biochem 67:148\u2013157. https:\/\/doi.org\/10.1002\/bab.1867","journal-title":"Biotech Appl Biochem"},{"key":"3371_CR28","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1016\/j.ijid.2020.04.059","volume":"96","author":"K Sakashita","year":"2020","unstructured":"Sakashita K, Takeuchi R, Takeda K, Takamori M, Ito K et al (2020) Ultrasensitive enzyme-linked immunosorbent assay for the detection of MPT64 secretory antigen to evaluate Mycobacterium tuberculosis viability in sputum. Int J Infect Dis 96:244\u2013256. https:\/\/doi.org\/10.1016\/j.ijid.2020.04.059","journal-title":"Int J Infect Dis"},{"key":"3371_CR29","volume-title":"Molecular Cloning: A Laboratory Manual","author":"J Sambrook","year":"1989","unstructured":"Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York"},{"key":"3371_CR30","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1007\/978-981-15-7470-2_17","volume-title":"Antioxidants in vegetables and nuts - Properties and health benefits","author":"V Sharma","year":"2020","unstructured":"Sharma V, Sharma L, Sandhu KS (2020) Cucumber (Cucumis sativus L.). In: Nayik GA, Gull A (eds) Antioxidants in vegetables and nuts - Properties and health benefits. Springer, Singapore, pp 333\u2013340"},{"key":"3371_CR32","doi-asserted-by":"publisher","first-page":"e1004446","DOI":"10.1371\/journal.ppat.1004446","volume":"10","author":"G Sreejit","year":"2014","unstructured":"Sreejit G, Ahmed A, Parveen N, Jha V, Valluri VL (2014) The ESAT-6 protein of\u00a0Mycobacterium tuberculosis\u00a0interacts with beta-2-microglobulin (\u03b22M) affecting antigen presentation function of macrophage. PLOS Pathog 10:e1004446. https:\/\/doi.org\/10.1371\/journal.ppat.1004446","journal-title":"PLOS Pathog"},{"key":"3371_CR33","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/316304","author":"EA Uvarova","year":"2013","unstructured":"Uvarova EA, Belavin PA, Permyakova NV, Zagorskaya AA, Nosareva OV, Kakimzhanova AA, Deineko EV (2013) Oral immunogenicity of plant-made Mycobacterium tuberculosis, ESAT6 and CFP10. Biomed Res Int. https:\/\/doi.org\/10.1155\/2013\/316304","journal-title":"Biomed Res Int"},{"key":"3371_CR34","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1079\/IVP2004602","volume":"41","author":"G Vengadesan","year":"2005","unstructured":"Vengadesan G, Anand RP, Selvaraj N, Perl-Treves R, Ganapthi A (2005) Transfer and expression of nptII and bar Genes in cucumber (Cucumber sativus L.). In Vitro Cell Dev Biol 41:17\u201321","journal-title":"In Vitro Cell Dev Biol"},{"key":"3371_CR35","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1111\/cei.13287","volume":"196","author":"J Wallis","year":"2019","unstructured":"Wallis J, Shenton DP, Carlisle RC (2019) Novel approaches for the design, delivery and administration of vaccine technologies. Clin Exp Immunol 196:189\u2013204","journal-title":"Clin Exp Immunol"},{"key":"3371_CR31","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/S2095-3119(14)60899-6","volume":"14","author":"SL Wang","year":"2015","unstructured":"Wang SL, Ku SS, Ye XG et al (2015) Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.). J Integr Agric 14:469\u2013482. https:\/\/doi.org\/10.1016\/S2095-3119(14)60899-6","journal-title":"J Integr Agric"},{"key":"3371_CR36","doi-asserted-by":"publisher","first-page":"52","DOI":"10.3390\/vaccines7020052","volume":"7","author":"N Wang","year":"2019","unstructured":"Wang N, Qiu C, Chen M, Liu T, Wang T (2019) Covering aluminum oxide nanoparticles with biocompatible materials to efficiently deliver subunit vaccines. Vaccines 7:52. https:\/\/doi.org\/10.3390\/vaccines7020052","journal-title":"Vaccines"},{"key":"3371_CR37","unstructured":"WHO (2022) Tuberculosis-key facts, file:\/\/\/C:\/Users\/Sony\/Desktop\/Jyoti-Sep,%202022\u20132\/Tuberculosis.html"},{"key":"3371_CR38","doi-asserted-by":"publisher","first-page":"e18073","DOI":"10.1097\/MD.0000000000018073","volume":"98","author":"T Xiao","year":"2019","unstructured":"Xiao T, Jiang Y, Li G, Pang H, Zhao L, Zhao X et al (2019) Polymorphism of MPT64 and PstS1 in Mycobacterium tuberculosis is not likely to affect relative immune reaction in human. Medicine (Baltimore) 98:e18073. https:\/\/doi.org\/10.1097\/MD.0000000000018073","journal-title":"Medicine (Baltimore)"},{"key":"3371_CR39","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1007\/s13562-020-00635-z","volume":"30","author":"H Yadav","year":"2021","unstructured":"Yadav H, Malik K, Parmar S, Kumar S, Jaiwal PK (2021) Generation of polyclonal antibodies against recombinant Agrobacterium tumefaciens decaprenyl diphosphate synthase produced in Escherichia coli. J Plant Biochem Biotech 30:487\u2013495. https:\/\/doi.org\/10.1007\/s13562-020-00635-z","journal-title":"J Plant Biochem Biotech"},{"key":"3371_CR40","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1093\/abbs\/gms068","volume":"44","author":"Y Zhang","year":"2012","unstructured":"Zhang Y, Chen S, Liu JY, Yuanlei Hu, Hong C (2012) Oral immunogenicity of potato-derived antigens to Mycobacterium tuberculosis in mice. Acta Biochim Biophys Sin 44:823\u2013830","journal-title":"Acta Biochim Biophys Sin"},{"key":"3371_CR41","doi-asserted-by":"publisher","DOI":"10.1038\/protex.2017.107","author":"Z Zhang","year":"2017","unstructured":"Zhang Z, Li X, Ma S, Shan N, Zhang X, Sui X (2017) A protocol for Agrobacterium- mediated transformation of cucumber (Cucumis sativus L.) from cotyledon explants. Protoc Exch. https:\/\/doi.org\/10.1038\/protex.2017.107","journal-title":"Protoc Exch"}],"container-title":["Biotechnology Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10529-023-03371-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10529-023-03371-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10529-023-03371-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T15:52:35Z","timestamp":1744213955000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10529-023-03371-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,19]]},"references-count":41,"journal-issue":{"issue":"5-6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["3371"],"URL":"https:\/\/doi.org\/10.1007\/s10529-023-03371-1","relation":{},"ISSN":["0141-5492","1573-6776"],"issn-type":[{"value":"0141-5492","type":"print"},{"value":"1573-6776","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,19]]},"assertion":[{"value":"20 November 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 March 2023","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 March 2023","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 April 2023","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no conflicting interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}