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Oxidation reactions showed that adding 2\u00a0mM CuSO<jats:sub>4<\/jats:sub> to the assay solution led to activating the laccase. To increase its initial activity, the rLac-<jats:italic>HhC125<\/jats:italic> was treated at 50\u00a0\u00b0C for 20\u00a0min before the assays, improving its performance by fourfold using the syringaldazine as a substrate. When treated with EDTA, methanol, ethanol, and DMSO, the rLac-<jats:italic>HhC125<\/jats:italic> maintained more than 80% of its original activity. Interestingly, the acetonitrile induced a twofold activity of the rLac-<jats:italic>HhC125<\/jats:italic>. The putative rLac-<jats:italic>HhC125<\/jats:italic> demonstrated a capability of efficient transformation of different organic compounds at pH 6, known as dye precursors, into coloured molecules.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>The rLac-<jats:italic>HhC125<\/jats:italic> was active at high temperatures and alkaline pH, exhibited tolerance to organic solvents, and efficiently transformed different hydroxy derivatives into coloured compounds, which indicates that it can be used in various biotechnological processes.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s10529-024-03532-w","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T09:15:45Z","timestamp":1730106945000},"page":"1199-1218","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Biochemical characterization of a recombinant laccase from Halalkalibacterium halodurans C-125 and its application in the biotransformation of organic compounds"],"prefix":"10.1007","volume":"46","author":[{"given":"Jihene","family":"Maati","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jolanta","family":"Polak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Janczarek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcin","family":"Gr\u0105z","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Issam","family":"Smaali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8222-4379","authenticated-orcid":false,"given":"Anna","family":"Jarosz-Wilko\u0142azka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,10,28]]},"reference":[{"key":"3532_CR1","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.femsle.2005.05.009","volume":"248","author":"M Akita","year":"2005","unstructured":"Akita M, Kayatama K, Hatada Y et al (2005) A novel \u03b2-glucanase gene from Bacillus halodurans C-125. 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