{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T19:52:34Z","timestamp":1793994754791,"version":"4.8.0"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["AMR\/ADHOC\/184\/2019-ECD-II"],"award-info":[{"award-number":["AMR\/ADHOC\/184\/2019-ECD-II"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biotechnol Lett"],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1007\/s10529-026-03693-w","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T08:59:39Z","timestamp":1770713979000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anti-quorum, anti biofilm activity of FDA approved drugs against P. aeruginosa: in silico and in vitro studies"],"prefix":"10.1007","volume":"48","author":[{"given":"Vivek","family":"Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anmol","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7060-4454","authenticated-orcid":false,"given":"Vishnu","family":"Agarwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,10]]},"reference":[{"key":"3693_CR1","unstructured":"A Simple Method for the Detection of Protease Activity on Agar Plates Using Bromocresolgreen Dye (2013) J Biochem Technol 4(3\u20132013): 628\u201330."},{"issue":"4","key":"3693_CR2","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1038\/nrmicro3232","volume":"12","author":"RC Allen","year":"2014","unstructured":"Allen RC, Popat R, Diggle SP, Brown SP (2014) Targeting virulence: can we make evolution-proof drugs? Nat Rev Microbiol 12(4):300\u2013308. https:\/\/doi.org\/10.1038\/nrmicro3232","journal-title":"Nat Rev Microbiol"},{"key":"3693_CR3","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-10-148","volume":"10","author":"F Babi\u0107","year":"2010","unstructured":"Babi\u0107 F, Venturi V, Maravi\u0107-Vlahovi\u010dek G (2010) Tobramycin at subinhibitory concentration inhibits the RhlI\/R quorum sensing system in a Pseudomonas aeruginosa environmental isolate. BMC Infect Dis 10:148. https:\/\/doi.org\/10.1186\/1471-2334-10-148","journal-title":"BMC Infect Dis"},{"issue":"11","key":"3693_CR4","doi-asserted-by":"publisher","first-page":"3630","DOI":"10.1128\/JB.187.11.3630-3635.2005","volume":"187","author":"F Bredenbruch","year":"2005","unstructured":"Bredenbruch F, Nimtz M, Wray V, Morr M, M\u00fcller R, H\u00e4ussler S (2005) Biosynthetic pathway of Pseudomonas aeruginosa 4-hydroxy-2-alkylquinolines. J Bacteriol 187(11):3630\u20133635. https:\/\/doi.org\/10.1128\/JB.187.11.3630-3635.2005","journal-title":"J Bacteriol"},{"issue":"6871","key":"3693_CR5","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1038\/415545a","volume":"415","author":"X Chen","year":"2002","unstructured":"Chen X, Schauder S, Potier N, Van Dorsselaer A, Pelczer I, Bassler BL, Hughson FM (2002) Structural identification of a bacterial quorum-sensing signal containing boron. Nature 415(6871):545\u2013549. https:\/\/doi.org\/10.1038\/415545a","journal-title":"Nature"},{"key":"3693_CR6","unstructured":"\u201cCommunication in Bacteria: An Ecological and Evolutionary Perspective | Nature Reviews Microbiology.\u201d https:\/\/www.nature.com\/articles\/nrmicro1383 (2024)."},{"key":"3693_CR7","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1007\/978-1-4939-2269-7_19","volume":"1263","author":"S Dallakyan","year":"2015","unstructured":"Dallakyan S, Olson AJ (2015) Small-molecule library screening by docking with PyRx. Methods Mol Biol 1263:243\u2013250. https:\/\/doi.org\/10.1007\/978-1-4939-2269-7_19","journal-title":"Methods Mol Biol"},{"issue":"2","key":"3693_CR8","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1111\/j.1462-2920.2008.01792.x","volume":"11","author":"TR de Kievit","year":"2009","unstructured":"de Kievit TR (2009) Quorum sensing in Pseudomonas aeruginosa biofilms. Environ Microbiol 11(2):279\u2013288. https:\/\/doi.org\/10.1111\/j.1462-2920.2008.01792.x","journal-title":"Environ Microbiol"},{"issue":"5","key":"3693_CR9","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.1073\/pnas.0307694100","volume":"101","author":"E D\u00e9ziel","year":"2004","unstructured":"D\u00e9ziel E, L\u00e9pine F, Milot S, He J, Mindrinos MN, Tompkins RG, Rahme LG (2004) Analysis of Pseudomonas aeruginosa 4-hydroxy-2-alkylquinolines (HAQs) reveals a role for 4-hydroxy-2-heptylquinoline in cell-to-cell communication. Proc Natl Acad Sci U S A 101(5):1339\u20131344. https:\/\/doi.org\/10.1073\/pnas.0307694100","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2\u20133","key":"3693_CR10","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.ijmm.2006.01.038","volume":"296","author":"SP Diggle","year":"2006","unstructured":"Diggle SP, Cornelis P, Williams P, C\u00e1mara M (2006) 4-quinolone signalling in Pseudomonas aeruginosa: old molecules, new perspectives. Int J Med Microbiol 296(2\u20133):83\u201391. https:\/\/doi.org\/10.1016\/j.ijmm.2006.01.038","journal-title":"Int J Med Microbiol"},{"key":"3693_CR11","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1146\/annurev.micro.51.1.527","volume":"51","author":"GM Dunny","year":"1997","unstructured":"Dunny GM, Leonard BA (1997) Cell-cell communication in gram-positive bacteria. Annu Rev Microbiol 51:527\u2013564. https:\/\/doi.org\/10.1146\/annurev.micro.51.1.527","journal-title":"Annu Rev Microbiol"},{"issue":"2","key":"3693_CR12","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.idcr.2015.01.004","volume":"2","author":"MJ Gale","year":"2015","unstructured":"Gale MJ, Maritato MS, Chen Y-L, Abdulateef SS, Ruiz JE (2015) Pseudomonas aeruginosa causing inflammatory mass of the nasopharynx in an immunocompromised HIV infected patient: a mimic of malignancy. Idcases 2(2):40\u201343. https:\/\/doi.org\/10.1016\/j.idcr.2015.01.004","journal-title":"Idcases"},{"issue":"3","key":"3693_CR13","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1128\/iai.62.3.915-921.1994","volume":"62","author":"SP Hawser","year":"1994","unstructured":"Hawser SP, Douglas LJ (1994) Biofilm formation by Candida species on the surface of catheter materials in vitro. Infect Immun 62(3):915\u2013921. https:\/\/doi.org\/10.1128\/iai.62.3.915-921.1994","journal-title":"Infect Immun"},{"issue":"1","key":"3693_CR14","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1099\/00221287-148-1-87","volume":"148","author":"M Hentzer","year":"2002","unstructured":"Hentzer M, Riedel K, Rasmussen TB, Heydorn A, Andersen JB, Parsek MR, Rice SA et al (2002) Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound. Microbiology 148(1):87\u2013102. https:\/\/doi.org\/10.1099\/00221287-148-1-87","journal-title":"Microbiology"},{"issue":"8","key":"3693_CR15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135495","volume":"10","author":"M Kalia","year":"2015","unstructured":"Kalia M, Yadav VK, Singh PK, Sharma D, Pandey H, Narvi SS, Agarwal V (2015) Effect of cinnamon oil on quorum sensing-controlled virulence factors and biofilm formation in Pseudomonas aeruginosa. PLoS ONE 10(8):e0135495. https:\/\/doi.org\/10.1371\/journal.pone.0135495","journal-title":"PLoS ONE"},{"issue":"39","key":"3693_CR16","doi-asserted-by":"publisher","first-page":"35866","DOI":"10.1021\/acsomega.3c03507","volume":"8","author":"P Khan","year":"2023","unstructured":"Khan P, Waheed A, Azeem M, Parveen A, Yameen MA, Iqbal J, Ali M et al (2023) Essential oil from Tagetes minuta has antiquorum sensing and antibiofilm potential against Pseudomonas aeruginosa strain PAO1. ACS Omega 8(39):35866\u201335873. https:\/\/doi.org\/10.1021\/acsomega.3c03507","journal-title":"ACS Omega"},{"issue":"3","key":"3693_CR17","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1016\/0003-2697(68)90154-1","volume":"24","author":"CA Knutson","year":"1968","unstructured":"Knutson CA, Jeanes A (1968) A new modification of the carbazole analysis: application to heteropolysaccharides. Anal Biochem 24(3):470\u2013481. https:\/\/doi.org\/10.1016\/0003-2697(68)90154-1","journal-title":"Anal Biochem"},{"issue":"4","key":"3693_CR18","doi-asserted-by":"publisher","first-page":"4016","DOI":"10.3390\/s120404016","volume":"12","author":"T Krishnan","year":"2012","unstructured":"Krishnan T, Yin W-F, Chan K-G (2012) Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa PAO1 by Ayurveda spice clove (Syzygium aromaticum) bud extract. Sensors 12(4):4016\u20134030. https:\/\/doi.org\/10.3390\/s120404016","journal-title":"Sensors"},{"key":"3693_CR19","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MB-0011-2014","author":"DH Limoli","year":"2015","unstructured":"Limoli DH, Jones CJ, Wozniak DJ (2015) Bacterial extracellular polysaccharides in biofilm formation and function. Microbiol Spectr. https:\/\/doi.org\/10.1128\/microbiolspec.MB-0011-2014","journal-title":"Microbiol Spectr"},{"issue":"4","key":"3693_CR20","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods (San Diego, Calif) 25(4):402\u2013408. https:\/\/doi.org\/10.1006\/meth.2001.1262","journal-title":"Methods (San Diego, Calif)"},{"issue":"12","key":"3693_CR21","doi-asserted-by":"publisher","first-page":"3703","DOI":"10.1099\/00221287-143-12-3703","volume":"143","author":"KH McClean","year":"1997","unstructured":"McClean KH, Winson MK, Fish L, Taylor A, Chhabra SR, Camara M, Daykin M, Lamb JH, Swift S, Bycroft BW, Stewart GS (1997) Quorum sensing and chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology 143(12):3703\u20133711. https:\/\/doi.org\/10.1099\/00221287-143-12-3703","journal-title":"Microbiology"},{"key":"3693_CR22","unstructured":"Miller JH (1972) Experiments in molecular genetics. [Cold Spring Harbor, N.Y.]: Cold Spring Harbor Laboratory."},{"issue":"11","key":"3693_CR23","doi-asserted-by":"publisher","first-page":"1905","DOI":"10.1002\/jor.23218","volume":"34","author":"M Morgenstern","year":"2016","unstructured":"Morgenstern M, Post V, Erichsen C, Hungerer S, B\u00fchren V, Militz M, Richards RG, Moriarty TF (2016) Biofilm formation increases treatment failure in Staphylococcus epidermidis device-related osteomyelitis of the lower extremity in human patients. J Orthop Res 34(11):1905\u20131913. https:\/\/doi.org\/10.1002\/jor.23218","journal-title":"J Orthop Res"},{"issue":"1","key":"3693_CR24","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1002\/0471250953.bi0814s24","volume":"24","author":"GM Morris","year":"2008","unstructured":"Morris GM, Huey R, Olson AJ (2008) Using AutoDock for ligand-receptor docking. Curr Protocols Bioinformat 24(1):8\u201314. https:\/\/doi.org\/10.1002\/0471250953.bi0814s24","journal-title":"Curr Protocols Bioinformat"},{"issue":"1","key":"3693_CR25","doi-asserted-by":"publisher","first-page":"L94","DOI":"10.1152\/ajplung.00025.2004","volume":"287","author":"YQ O\u2019Malley","year":"2004","unstructured":"O\u2019Malley YQ, Reszka KJ, Spitz DR, Denning GM, Britigan BE (2004) Pseudomonas aeruginosa pyocyanin directly oxidizes glutathione and decreases its levels in airway epithelial cells. Am J Physiol Lung Cell Mol Physiol 287(1):L94-103. https:\/\/doi.org\/10.1152\/ajplung.00025.2004","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"3693_CR26","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1146\/annurev.micro.54.1.49","volume":"54","author":"G O\u2019Toole","year":"2000","unstructured":"O\u2019Toole G, Kaplan HB, Kolter R (2000) Biofilm formation as microbial development. Annu Rev Microbiol 54:49\u201379. https:\/\/doi.org\/10.1146\/annurev.micro.54.1.49","journal-title":"Annu Rev Microbiol"},{"issue":"7","key":"3693_CR27","doi-asserted-by":"publisher","first-page":"2044","DOI":"10.1128\/jb.176.7.2044-2054.1994","volume":"176","author":"UA Ochsner","year":"1994","unstructured":"Ochsner UA, Koch AK, Fiechter A, Reiser J (1994) Isolation and characterization of a regulatory gene affecting rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa. J Bacteriol 176(7):2044\u20132054. https:\/\/doi.org\/10.1128\/jb.176.7.2044-2054.1994","journal-title":"J Bacteriol"},{"key":"3693_CR28","unstructured":"Organization, World Health (2017) Prioritization of Pathogens to Guide Discovery, Research and Development of New Antibiotics for Drug-Resistant Bacterial Infections, Including Tuberculosis. World Health Organization. https:\/\/iris.who.int\/handle\/10665\/311820 (May 12, 2024)."},{"issue":"5111","key":"3693_CR29","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.1126\/science.8493556","volume":"260","author":"L Passador","year":"1993","unstructured":"Passador L, Cook JM, Gambello MJ, Rust L, Iglewski BH (1993) Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication. Science 260(5111):1127\u20131130. https:\/\/doi.org\/10.1126\/science.8493556","journal-title":"Science"},{"issue":"2","key":"3693_CR30","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1111\/j.1574-6976.2012.00345.x","volume":"37","author":"CS Pereira","year":"2013","unstructured":"Pereira CS, Thompson JA, Xavier KB (2013) AI-2-mediated signalling in bacteria. FEMS Microbiol Rev 37(2):156\u2013181. https:\/\/doi.org\/10.1111\/j.1574-6976.2012.00345.x","journal-title":"FEMS Microbiol Rev"},{"issue":"11","key":"3693_CR31","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a012427","volume":"2","author":"ST Rutherford","year":"2012","unstructured":"Rutherford ST, Bassler BL (2012) Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring Harb Perspect Med 2(11):a012427. https:\/\/doi.org\/10.1101\/cshperspect.a012427","journal-title":"Cold Spring Harb Perspect Med"},{"issue":"7","key":"3693_CR32","doi-asserted-by":"publisher","first-page":"3974","DOI":"10.1128\/jb.172.7.3974-3979.1990","volume":"172","author":"J Slock","year":"1990","unstructured":"Slock J, VanRiet D, Kolibachuk D, Greenberg EP (1990) Critical regions of the Vibrio fischeri luxR protein defined by mutational analysis. J Bacteriol 172(7):3974\u20133979. https:\/\/doi.org\/10.1128\/jb.172.7.3974-3979.1990","journal-title":"J Bacteriol"},{"issue":"1","key":"3693_CR33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1574-6968.2006.00501.x","volume":"266","author":"L Steindler","year":"2007","unstructured":"Steindler L, Venturi V (2007) Detection of quorum-sensing N-acyl homoserine lactone signal molecules by bacterial biosensors. FEMS Microbiol Lett 266(1):1\u20139. https:\/\/doi.org\/10.1111\/j.1574-6968.2006.00501.x","journal-title":"FEMS Microbiol Lett"},{"issue":"26","key":"3693_CR34","doi-asserted-by":"publisher","first-page":"12619","DOI":"10.1073\/pnas.91.26.12619","volume":"91","author":"AM Stevens","year":"1994","unstructured":"Stevens AM, Dolan KM, Greenberg EP (1994) Synergistic binding of the Vibrio fischeri LuxR transcriptional activator domain and RNA polymerase to the lux promoter region. Proc Natl Acad Sci U S A 91(26):12619\u201312623. https:\/\/doi.org\/10.1073\/pnas.91.26.12619","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"13","key":"3693_CR35","doi-asserted-by":"publisher","first-page":"4372","DOI":"10.1128\/JB.187.13.4372-4380.2005","volume":"187","author":"DS Wade","year":"2005","unstructured":"Wade DS, Calfee MW, Rocha ER, Ling EA, Engstrom E, Coleman JP, Pesci EC (2005) Regulation of Pseudomonas quinolone signal synthesis in Pseudomonas aeruginosa. J Bacteriol 187(13):4372\u20134380. https:\/\/doi.org\/10.1128\/JB.187.13.4372-4380.2005","journal-title":"J Bacteriol"},{"issue":"11","key":"3693_CR36","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1001\/archotol.131.11.983","volume":"131","author":"EW Wang","year":"2005","unstructured":"Wang EW, Jung JY, Pashia ME, Nason R, Scholnick S, Chole RA (2005) Otopathogenic Pseudomonas aeruginosa strains as competent biofilm formers. Arch Otolaryngol Head Neck Surg 131(11):983\u2013989. https:\/\/doi.org\/10.1001\/archotol.131.11.983","journal-title":"Arch Otolaryngol Head Neck Surg"},{"issue":"3","key":"3693_CR37","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01864-19","volume":"58","author":"MP Weinstein","year":"2020","unstructured":"Weinstein MP, Lewis JS (2020) The clinical and laboratory standards institute subcommittee on antimicrobial susceptibility testing: background, organization, functions, and processes. J Clin Microbiol 58(3):e01864-19. https:\/\/doi.org\/10.1128\/JCM.01864-19","journal-title":"J Clin Microbiol"},{"issue":"6","key":"3693_CR38","doi-asserted-by":"publisher","first-page":"1689","DOI":"10.1111\/j.1365-2958.2006.05462.x","volume":"62","author":"G Xiao","year":"2006","unstructured":"Xiao G, D\u00e9ziel E, He J, L\u00e9pine F, Lesic B, Castonguay M-H, Milot S et al (2006) MvfR, a key Pseudomonas aeruginosa pathogenicity LTTR-class regulatory protein, has dual ligands. Mol Microbiol 62(6):1689\u20131699. https:\/\/doi.org\/10.1111\/j.1365-2958.2006.05462.x","journal-title":"Mol Microbiol"}],"container-title":["Biotechnology Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10529-026-03693-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10529-026-03693-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10529-026-03693-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T04:27:27Z","timestamp":1775708847000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10529-026-03693-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,10]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["3693"],"URL":"https:\/\/doi.org\/10.1007\/s10529-026-03693-w","relation":{},"ISSN":["0141-5492","1573-6776"],"issn-type":[{"value":"0141-5492","type":"print"},{"value":"1573-6776","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,10]]},"assertion":[{"value":"23 June 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 December 2025","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 January 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 February 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interest"}}],"article-number":"35"}}