{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T04:57:51Z","timestamp":1780981071610,"version":"3.54.1"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2022YFB4600600"],"award-info":[{"award-number":["2022YFB4600600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"crossref","award":["2020B1515120001"],"award-info":[{"award-number":["2020B1515120001"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biotechnol Lett"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1007\/s10529-026-03733-5","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T16:34:54Z","timestamp":1777048494000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and validation of a novel latex particle-enhanced turbidimetric immunoassay for fecal calprotectin"],"prefix":"10.1007","volume":"48","author":[{"given":"Zhe","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feihu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaodong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhua","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"key":"3733_CR1","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/bs.acc.2018.07.005","volume":"87","author":"RM Ayling","year":"2018","unstructured":"Ayling RM, Kok K (2018) Fecal calprotectin. Adv Clin Chem 87:161\u2013190. https:\/\/doi.org\/10.1016\/bs.acc.2018.07.005","journal-title":"Adv Clin Chem"},{"issue":"11","key":"3733_CR2","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1038\/s41575-022-00634-6","volume":"19","author":"TH Bisgaard","year":"2022","unstructured":"Bisgaard TH, Allin KH, Keefer L, Ananthakrishnan AN, Jess T (2022) Depression and anxiety in inflammatory bowel disease: epidemiology, mechanisms and treatment. Nat Rev Gastroenterol Hepatol 19(11):717\u2013726. https:\/\/doi.org\/10.1038\/s41575-022-00634-6","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"3733_CR3","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2022.1103713","volume":"10","author":"SM Borowitz","year":"2022","unstructured":"Borowitz SM (2022) The epidemiology of inflammatory bowel disease: clues to pathogenesis? Front Pediatr 10:1103713. https:\/\/doi.org\/10.3389\/fped.2022.1103713","journal-title":"Front Pediatr"},{"key":"3733_CR4","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2021.462000","volume":"1642","author":"X Deng","year":"2021","unstructured":"Deng X, Zeng M, Wang X, Liu J, Ma Y, Wang X, Xu L (2021) Preparation and characterization of cyclic citrullinated peptide-immobilized latex beads for measurement of anti-citrillinated protein antibody through latex particle-enhanced turbidimetric immunoassay. J Chromatogr A 1642:462000. https:\/\/doi.org\/10.1016\/j.chroma.2021.462000","journal-title":"J Chromatogr A"},{"issue":"1","key":"3733_CR5","doi-asserted-by":"publisher","first-page":"24","DOI":"10.2174\/156652413804486214","volume":"13","author":"R Donato","year":"2013","unstructured":"Donato R, Cannon BR, Sorci G, Riuzzi F, Hsu K, Weber DJ, Geczy CL (2013) Functions of S100 proteins. Curr Mol Med 13(1):24\u201357","journal-title":"Curr Mol Med"},{"issue":"12","key":"3733_CR6","doi-asserted-by":"publisher","first-page":"7706","DOI":"10.1016\/S0021-9258(20)89506-4","volume":"266","author":"J Edgeworth","year":"1991","unstructured":"Edgeworth J, Gorman M, Bennett R, Freemont P, Hogg N (1991) Identification of p8,14 as a highly abundant heterodimeric calcium binding protein complex of myeloid cells. J Biol Chem 266(12):7706\u20137713","journal-title":"J Biol Chem"},{"issue":"10","key":"3733_CR7","doi-asserted-by":"publisher","first-page":"1978","DOI":"10.1136\/gutjnl-2021-324855","volume":"70","author":"A Jukic","year":"2021","unstructured":"Jukic A, Bakiri L, Wagner EF, Tilg H, Adolph TE (2021) Calprotectin: from biomarker to biological function. Gut 70(10):1978\u20131988. https:\/\/doi.org\/10.1136\/gutjnl-2021-324855","journal-title":"Gut"},{"issue":"1","key":"3733_CR8","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1038\/s41575-020-00360-x","volume":"18","author":"GG Kaplan","year":"2021","unstructured":"Kaplan GG, Windsor JW (2021) The four epidemiological stages in the global evolution of inflammatory bowel disease. Nat Rev Gastroenterol Hepatol 18(1):56\u201366. https:\/\/doi.org\/10.1038\/s41575-020-00360-x","journal-title":"Nat Rev Gastroenterol Hepatol"},{"issue":"Suppl 3","key":"3733_CR9","doi-asserted-by":"publisher","first-page":"s1","DOI":"10.1136\/gutjnl-2019-318484","volume":"68","author":"CA Lamb","year":"2019","unstructured":"Lamb CA, Kennedy NA, Raine T, Hendy PA, Smith PJ, Limdi JK, Hayee B, Lomer MCE, Parkes GC, Selinger C, Barrett KJ, Davies RJ, Bennett C, Gittens S, Dunlop MG, Faiz O, Fraser A, Garrick V, Johnston PD et al (2019) British society of gastroenterology consensus guidelines on the management of inflammatory bowel disease in adults. Gut 68(Suppl 3):s1\u2013s106. https:\/\/doi.org\/10.1136\/gutjnl-2019-318484","journal-title":"Gut"},{"issue":"5","key":"3733_CR10","doi-asserted-by":"publisher","first-page":"1856","DOI":"10.1002\/med.21893","volume":"42","author":"D Liu","year":"2022","unstructured":"Liu D, Saikam V, Skrada KA, Merlin D, Iyer SS (2022) Inflammatory bowel disease biomarkers. Med Res Rev 42(5):1856\u20131887. https:\/\/doi.org\/10.1002\/med.21893","journal-title":"Med Res Rev"},{"issue":"2","key":"3733_CR11","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1007\/s10529-025-03564-w","volume":"47","author":"Y Liu","year":"2025","unstructured":"Liu Y, Li M, Zhang H, Gao L, Liu J, Hou Y, Xu J (2025) Development of a fully automated latex-enhanced immunoturbidimetric method for quantitative serum Lp(a) measurement. Biotechnol Lett 47(2):31. https:\/\/doi.org\/10.1007\/s10529-025-03564-w","journal-title":"Biotechnol Lett"},{"key":"3733_CR12","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.cca.2015.01.016","volume":"442","author":"T Machida","year":"2015","unstructured":"Machida T, Miyashita K, Sone T, Tanaka S, Nakajima K, Saito M, Stanhope K, Havel P, Sumino H, Murakami M (2015) Determination of serum lipoprotein lipase using a latex particle-enhanced turbidimetric immunoassay with an automated analyzer. Clin Chim Acta 442:130\u2013135. https:\/\/doi.org\/10.1016\/j.cca.2015.01.016","journal-title":"Clin Chim Acta"},{"issue":"10","key":"3733_CR13","doi-asserted-by":"publisher","first-page":"1400","DOI":"10.1136\/annrheumdis-2022-222229","volume":"81","author":"LF Mellor","year":"2022","unstructured":"Mellor LF, Gago-Lopez N, Bakiri L, Schmidt FN, Busse B, Rauber S, Jimenez M, Meg\u00edas D, Oterino-Sogo S, Sanchez-Prieto R, Grivennikov S, Pu X, Oxford J, Ramming A, Schett G, Wagner EF (2022) Keratinocyte-derived S100A9 modulates neutrophil infiltration and affects psoriasis-like skin and joint disease. Ann Rheum Dis 81(10):1400\u20131408. https:\/\/doi.org\/10.1136\/annrheumdis-2022-222229","journal-title":"Ann Rheum Dis"},{"issue":"3","key":"3733_CR14","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1038\/ajg.2015.6","volume":"110","author":"SB Menees","year":"2015","unstructured":"Menees SB, Powell C, Kurlander J, Goel A, Chey WD (2015) A meta-analysis of the utility of C-reactive protein, erythrocyte sedimentation rate, fecal calprotectin, and fecal lactoferrin to exclude inflammatory bowel disease in adults with IBS. Am J Gastroenterol 110(3):444\u2013454. https:\/\/doi.org\/10.1038\/ajg.2015.6","journal-title":"Am J Gastroenterol"},{"issue":"2","key":"3733_CR15","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1016\/j.gie.2024.08.034","volume":"101","author":"B Shen","year":"2025","unstructured":"Shen B, Abreu MT, Cohen ER, Farraye FA, Fischer M, Feuerstadt P, Kapur S, Ko HM, Kochhar GS, Liu X, Mahadevan U, McBride DL, Navaneethan U, Regueiro M, Ritter T, Sharma P, Lichtenstein GR (2025) Endoscopic diagnosis and management of adult inflammatory bowel disease: a consensus document from the American Society for Gastrointestinal Endoscopy IBD Endoscopy Consensus Panel. Gastrointest Endosc 101(2):295\u2013314. https:\/\/doi.org\/10.1016\/j.gie.2024.08.034","journal-title":"Gastrointest Endosc"},{"issue":"7","key":"3733_CR16","doi-asserted-by":"publisher","first-page":"1248","DOI":"10.1093\/clinchem\/37.7.1248","volume":"37","author":"H Thakkar","year":"1991","unstructured":"Thakkar H, Davey CL, Medcalf EA, Skingle L, Craig AR, Newman DJ, Price CP (1991) Stabilization of turbidimetric immunoassay by covalent coupling of antibody to latex particles. Clin Chem 37(7):1248\u20131251","journal-title":"Clin Chem"},{"key":"3733_CR17","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2022.106782","volume":"238","author":"C Tomckowiack","year":"2023","unstructured":"Tomckowiack C, Ramirez-Reveco A, Henr\u00edquez C, Salgado M (2023) Development and evaluation of polyclonal antibody-based antigen detection ELISA and dot blot assays as a less costly diagnostic alternative for pathogenic Leptospira infection. Acta Trop 238:106782. https:\/\/doi.org\/10.1016\/j.actatropica.2022.106782","journal-title":"Acta Trop"},{"issue":"5","key":"3733_CR18","doi-asserted-by":"publisher","first-page":"1570","DOI":"10.1053\/j.gastro.2020.12.031","volume":"160","author":"D Turner","year":"2021","unstructured":"Turner D, Ricciuto A, Lewis A, D\u2019Amico F, Dhaliwal J, Griffiths AM, Bettenworth D, Sandborn WJ, Sands BE, Reinisch W, Sch\u00f6lmerich J, Bemelman W, Danese S, Mary JY, Rubin D, Colombel JF, Peyrin-Biroulet L, Dotan I, Abreu MT, Dignass A (2021) STRIDE-II: an update on the Selecting Therapeutic Targets in Inflammatory Bowel Disease (STRIDE) Initiative of the International Organization for the Study of IBD (IOIBD): determining therapeutic goals for treat-to-target strategies in IBD. Gastroenterology 160(5):1570\u20131583. https:\/\/doi.org\/10.1053\/j.gastro.2020.12.031","journal-title":"Gastroenterology"},{"key":"3733_CR19","doi-asserted-by":"publisher","first-page":"106793","DOI":"10.1016\/j.actatropica.2022.106793","volume":"238","author":"X Wang","year":"2023","unstructured":"Wang X, Dai Y, Zhao S, Tang J, Zhu Y (2023) SjHSP90 recombinant protein and its possible application in the diagnosis of schistosomiasis and treatment efficacy monitoring. Acta Tropica 238:106793. https:\/\/doi.org\/10.1016\/j.actatropica.2022.106793","journal-title":"Acta Tropica"},{"key":"3733_CR20","doi-asserted-by":"publisher","DOI":"10.1038\/srep40090","volume":"7","author":"Y Xia","year":"2017","unstructured":"Xia Y, Shen H, Zhu Y, Xu H, Li Z, Si J (2017) A sensitive three monoclonal antibodies based automatic latex particle-enhanced turbidimetric immunoassay for Golgi protein 73 detection. Sci Rep 7:40090. https:\/\/doi.org\/10.1038\/srep40090","journal-title":"Sci Rep"},{"issue":"Pt 2","key":"3733_CR21","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.1016\/j.semcancer.2021.12.012","volume":"86","author":"LY Xia","year":"2022","unstructured":"Xia LY, Tang YN, Zhang J, Dong TY, Zhou RX (2022) Advances in the DNA nanotechnology for the cancer biomarkers analysis: attributes and applications. Semin Cancer Biol 86(Pt 2):1105\u20131119. https:\/\/doi.org\/10.1016\/j.semcancer.2021.12.012","journal-title":"Semin Cancer Biol"},{"issue":"5","key":"3733_CR22","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.1007\/s40744-024-00709-7","volume":"11","author":"A Zabotti","year":"2024","unstructured":"Zabotti A, Cabas N, Cacioppo S, Zoratti C, Giovannini I, Berretti D, Luchetti MM, De Vita S, Quartuccio L, Terrosu G, Marino M (2024) The challenge of IBD-related arthritis screening questionnaires in early and predominantly entheseal phenotypes. Rheumatol Ther 11(5):1321\u20131331. https:\/\/doi.org\/10.1007\/s40744-024-00709-7","journal-title":"Rheumatol Ther"},{"key":"3733_CR23","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1146\/annurev-biochem-062917-012312","volume":"87","author":"EM Zygiel","year":"2018","unstructured":"Zygiel EM, Nolan EM (2018) Transition metal sequestration by the host-defense protein Calprotectin. Annu Rev Biochem 87:621\u2013643. https:\/\/doi.org\/10.1146\/annurev-biochem-062917-012312","journal-title":"Annu Rev Biochem"}],"container-title":["Biotechnology Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10529-026-03733-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10529-026-03733-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10529-026-03733-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T04:05:40Z","timestamp":1780977940000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10529-026-03733-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,24]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["3733"],"URL":"https:\/\/doi.org\/10.1007\/s10529-026-03733-5","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-8411228\/v1","asserted-by":"object"}]},"ISSN":["0141-5492","1573-6776"],"issn-type":[{"value":"0141-5492","type":"print"},{"value":"1573-6776","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,24]]},"assertion":[{"value":"20 December 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 April 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 April 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 April 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"All the procedures and studies involving human participants were performed in accordance with the ethical standards of the institutional and\/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. The experimental protocols and procedures were approved by Nanjing Drum Tower Hospital (approval number: FM-M7-2023051901). The stool sample was collected from Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School. The project was conducted from November 5, 2024 to March 30, 2025, with written informed consent obtained from all participants in accordance with ethical guidelines.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval and consent to participate"}},{"value":"The authors affirm that human research participants provided informed consent for publication.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}],"article-number":"64"}}