{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T17:28:31Z","timestamp":1793899711666,"version":"4.8.0"},"reference-count":24,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100003705","name":"Korea Institute of Toxicology","doi-asserted-by":"publisher","award":["1711195889"],"award-info":[{"award-number":["1711195889"]}],"id":[{"id":"10.13039\/501100003705","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003052","name":"Ministry of Trade, Industry and Energy","doi-asserted-by":"publisher","award":["RS-2024-00448560"],"award-info":[{"award-number":["RS-2024-00448560"]}],"id":[{"id":"10.13039\/501100003052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013129","name":"Ministry of SMEs and Startups","doi-asserted-by":"publisher","award":["RS-2025-25465822"],"award-info":[{"award-number":["RS-2025-25465822"]}],"id":[{"id":"10.13039\/501100013129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biotechnol Lett"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1007\/s10529-026-03780-y","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:11:21Z","timestamp":1788268281000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A mechanistic evaluation of sodium channel modulators in hiPSC-derived cardiomyocytes using fluorescence-based sodium flux imaging"],"prefix":"10.1007","volume":"48","author":[{"given":"Su-Bin","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaehun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieun","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ara","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun Hee","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eunho","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwan","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jina","family":"Ryu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki-Suk","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Choongseong","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7470-018X","authenticated-orcid":false,"given":"Dong-Hun","family":"Woo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"3780_CR1","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1016\/j.cardiores.2007.07.019","volume":"76","author":"H Abriel","year":"2007","unstructured":"Abriel H (2007) Roles and regulation of the cardiac sodium channel Nav1.5: recent insights from experimental studies. Cardiovasc Res 76:381\u2013389. https:\/\/doi.org\/10.1016\/j.cardiores.2007.07.019","journal-title":"Cardiovasc Res"},{"key":"3780_CR2","doi-asserted-by":"publisher","DOI":"10.1177\/2398212818776561","author":"LA Annecchino","year":"2018","unstructured":"Annecchino LA, Schultz SR (2018) Progress in automating patch clamp cellular physiology. Brain Neurosci Adv. https:\/\/doi.org\/10.1177\/2398212818776561","journal-title":"Brain Neurosci Adv"},{"key":"3780_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/cts.70098","volume":"17","author":"KW Chaudhary","year":"2024","unstructured":"Chaudhary KW, Clancy CE, Yang PC et al (2024) An overview of drug-induced sodium channel blockade and changes in cardiac conduction: implications for drug safety. Clin Transl Sci 17:1\u201311. https:\/\/doi.org\/10.1111\/cts.70098","journal-title":"Clin Transl Sci"},{"key":"3780_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0166143","volume":"11","author":"I El-Battrawy","year":"2016","unstructured":"El-Battrawy I, Lang S, Zhao Z et al (2016) Hyperthermia influences the effects of sodium channel blocking drugs in human-induced pluripotent stem cell-derived cardiomyocytes. PLoS ONE 11:1\u201318. https:\/\/doi.org\/10.1371\/journal.pone.0166143","journal-title":"PLoS ONE"},{"key":"3780_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fphar.2012.00006","volume":"3","author":"G Erdemli","year":"2012","unstructured":"Erdemli G, Kim AM, Ju H et al (2012) Cardiac safety implications of hNav1.5 blockade and a framework for pre-clinical evaluation. Front Pharmacol 3:1\u20139. https:\/\/doi.org\/10.3389\/fphar.2012.00006","journal-title":"Front Pharmacol"},{"key":"3780_CR6","doi-asserted-by":"publisher","first-page":"H12","DOI":"10.1152\/ajpheart.00631.2023","volume":"327","author":"D Guerrelli","year":"2024","unstructured":"Guerrelli D, Pressman J, Salameh S, Posnack N (2024) HiPSC-CM electrophysiology: impact of temporal changes and study parameters on experimental reproducibility. Am J Physiol\u2013hear Circ Physiol 327:H12\u2013H27. https:\/\/doi.org\/10.1152\/ajpheart.00631.2023","journal-title":"Am J Physiol\u2013hear Circ Physiol"},{"key":"3780_CR7","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1159\/000329966","volume":"27","author":"L Guo","year":"2011","unstructured":"Guo L, Qian JY, Abrams R et al (2011) The electrophysiological effects of cardiac glycosides in human iPSC-derived cardiomyocytes and in guinea pig isolated hearts. Cell Physiol Biochem 27:453\u2013462. https:\/\/doi.org\/10.1159\/000329966","journal-title":"Cell Physiol Biochem"},{"key":"3780_CR8","doi-asserted-by":"publisher","first-page":"11.18.1","DOI":"10.1002\/0471141755.ph1118s71","volume":"71","author":"K Harris","year":"2015","unstructured":"Harris K (2015) A human induced pluripotent stem cell\u2212derived cardiomyocyte (hiPSC-CM) multielectrode array assay for preclinical cardiac electrophysiology safety screening. Curr Protoc Pharmacol 71:11.18.1-11.18.15. https:\/\/doi.org\/10.1002\/0471141755.ph1118s71","journal-title":"Curr Protoc Pharmacol"},{"key":"3780_CR9","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1093\/toxsci\/kft113","volume":"134","author":"K Harris","year":"2013","unstructured":"Harris K, Aylott M, Cui Y et al (2013) Comparison of electrophysiological data from human-induced pluripotent stem cell-derived cardiomyocytes to functional preclinical safety assays. Toxicol Sci 134:412\u2013426. https:\/\/doi.org\/10.1093\/toxsci\/kft113","journal-title":"Toxicol Sci"},{"key":"3780_CR10","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-026-04361-8","author":"SB Kim","year":"2026","unstructured":"Kim SB, Lee J, An J, Cho A, Lee KH, Kim E et al (2026) Integrating high-fidelity hiPSC-cardiomyocytes with AI-driven modeling for enhanced proarrhythmic risk assessment. Arch Toxicol. https:\/\/doi.org\/10.1007\/s00204-026-04361-8","journal-title":"Arch Toxicol"},{"key":"3780_CR11","doi-asserted-by":"publisher","first-page":"1726","DOI":"10.1016\/j.hrthm.2008.09.008.Role","volume":"5","author":"W Lin","year":"2008","unstructured":"Lin W et al (2008) Role of late sodium current in modulating the proarrhythmic and antiarrhythmic effects of quinidine. Hear Rhythm 5:1726\u20131734. https:\/\/doi.org\/10.1016\/j.hrthm.2008.09.008.Role","journal-title":"Hear Rhythm"},{"key":"3780_CR12","doi-asserted-by":"publisher","first-page":"E1051","DOI":"10.1152\/ajpendo.1995.268.6.e1051","volume":"268","author":"X Lyon","year":"1995","unstructured":"Lyon X, Schutz Y, Buclin T et al (1995) Inhibition of Na+-K+-ATPase by digoxin and its relation with energy expenditure and nutrient oxidation rate. Am J Physiol\u2013endocrinol Metab 268:E1051\u2013E1056. https:\/\/doi.org\/10.1152\/ajpendo.1995.268.6.e1051","journal-title":"Am J Physiol\u2013endocrinol Metab"},{"key":"3780_CR13","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.scr.2017.11.003","volume":"25","author":"DC Miller","year":"2017","unstructured":"Miller DC, Harmer SC, Poliandri A et al (2017) Ajmaline blocks INa and IKr without eliciting differences between Brugada syndrome patient and control human pluripotent stem cell-derived cardiac clusters. Stem Cell Res 25:233\u2013244. https:\/\/doi.org\/10.1016\/j.scr.2017.11.003","journal-title":"Stem Cell Res"},{"key":"3780_CR14","doi-asserted-by":"publisher","first-page":"53","DOI":"10.3389\/fcvm.2021.782596","volume":"8","author":"MM Monasky","year":"2021","unstructured":"Monasky MM, Micaglio E, D\u2019imperio S, Pappone C (2021) The mechanism of ajmaline and thus brugada syndrome: not only the sodium channel! Front Cardiovasc Med 8:53. https:\/\/doi.org\/10.3389\/fcvm.2021.782596","journal-title":"Front Cardiovasc Med"},{"key":"3780_CR15","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1093\/toxsci\/kfv128","volume":"147","author":"Y Qu","year":"2015","unstructured":"Qu Y, Hugo MV (2015) Proarrhythmia risk assessment in human induced pluripotent stem cell-derived cardiomyocytes using the maestro MEA platform. Toxicol Sci 147:286\u2013295. https:\/\/doi.org\/10.1093\/toxsci\/kfv128","journal-title":"Toxicol Sci"},{"key":"3780_CR16","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1113\/jphysiol.2004.065003","volume":"560","author":"E Ramos","year":"2004","unstructured":"Ramos E, O\u2019Leary ME (2004) State-dependent trapping of flecainide in the cardiac sodium channel. J Physiol 560:37\u201349. https:\/\/doi.org\/10.1113\/jphysiol.2004.065003","journal-title":"J Physiol"},{"key":"3780_CR17","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2024.117939","volume":"114","author":"Y Ren","year":"2024","unstructured":"Ren Y, Anderson AT, Meyer G et al (2024) Digoxin and its Na+\/K+-ATPase-targeted actions on cardiovascular diseases and cancer. Bioorg Med Chem 114:117939. https:\/\/doi.org\/10.1016\/j.bmc.2024.117939","journal-title":"Bioorg Med Chem"},{"key":"3780_CR18","doi-asserted-by":"publisher","first-page":"56","DOI":"10.3390\/ijms25168688","volume":"25","author":"T Saito","year":"2024","unstructured":"Saito T, Suzuki M, Ohba A et al (2024) Enhanced late ina induces intracellular ion disturbances and automatic activity in the guinea pig pulmonary vein cardiomyocytes. Int J Mol Sci 25:56. https:\/\/doi.org\/10.3390\/ijms25168688","journal-title":"Int J Mol Sci"},{"key":"3780_CR19","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/0022-2828(83)90327-9","volume":"15","author":"J Sanchez-Chapula","year":"1983","unstructured":"Sanchez-Chapula J, Josephson IR (1983) Effect of phenytoin on the sodium current in isolated rat ventricular cells. J Mol Cell Cardiol 15:515\u2013522. https:\/\/doi.org\/10.1016\/0022-2828(83)90327-9","journal-title":"J Mol Cell Cardiol"},{"key":"3780_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-021-91528-1","volume":"11","author":"AT Ton","year":"2021","unstructured":"Ton AT, Nguyen W, Sweat K et al (2021) Arrhythmogenic and antiarrhythmic actions of late sustained sodium current in the adult human heart. Sci Rep 11:1\u201316. https:\/\/doi.org\/10.1038\/s41598-021-91528-1","journal-title":"Sci Rep"},{"key":"3780_CR21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0128653","volume":"10","author":"Y Wang","year":"2015","unstructured":"Wang Y, Mi J, Lu K et al (2015) Comparison of gating properties and use-dependent block of Nav1.5 and Nav1.7 channels by anti-arrhythmics mexiletine and lidocaine. PLoS ONE 10:1\u201320. https:\/\/doi.org\/10.1371\/journal.pone.0128653","journal-title":"PLoS ONE"},{"key":"3780_CR22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.12659\/MSM.924092","volume":"26","author":"M Wang","year":"2020","unstructured":"Wang M, Shi Y, Yao L et al (2020) Potential molecular mechanisms and drugs for aconitine-induced cardiotoxicity in zebrafish through RNA sequencing and bioinformatics analysis. Med Sci Monit 26:1\u201313. https:\/\/doi.org\/10.12659\/MSM.924092","journal-title":"Med Sci Monit"},{"key":"3780_CR23","doi-asserted-by":"publisher","first-page":"87","DOI":"10.2174\/1875397301206010087","volume":"6","author":"X Yajuan","year":"2013","unstructured":"Yajuan X, Xin L, Zhiyuan L (2013) A comparison of the performance and application differences between manual and automated patch-clamp techniques. Curr Chem Genomics 6:87\u201392. https:\/\/doi.org\/10.2174\/1875397301206010087","journal-title":"Curr Chem Genomics"},{"key":"3780_CR24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fchem.2024.1339364","volume":"12","author":"P Zhao","year":"2024","unstructured":"Zhao P, Tian Y, Geng Y et al (2024) Aconitine and its derivatives: bioactivities, structure-activity relationships and preliminary molecular mechanisms. Front Chem 12:1\u201322. https:\/\/doi.org\/10.3389\/fchem.2024.1339364","journal-title":"Front Chem"}],"container-title":["Biotechnology Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10529-026-03780-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10529-026-03780-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10529-026-03780-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T17:05:50Z","timestamp":1793898350000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10529-026-03780-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":24,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["3780"],"URL":"https:\/\/doi.org\/10.1007\/s10529-026-03780-y","relation":{},"ISSN":["0141-5492","1573-6776"],"issn-type":[{"value":"0141-5492","type":"print"},{"value":"1573-6776","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]},"assertion":[{"value":"30 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 June 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 August 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 September 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All the authors declare that there is no competing interest.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"109"}}