{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:44:29Z","timestamp":1792554269368,"version":"4.5.0"},"reference-count":92,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100002352","name":"Ain Shams University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002352","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biodegradation"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>One of the most common soil pollutants on a global scale is fuel, which is fundamental for daily activities. Biodegradation has been regarded as an ideal remediation technique for hydrocarbon pollution. We investigated the potential of 28 <jats:italic>Streptomyces<\/jats:italic> species inhabiting different hydrocarbon-polluted soils for the biodegradation of petroleum. The tested isolates were cultured on mineral salts broth containing 2% crude oil as the sole carbon source. Gravimetric analysis of residual crude oil was performed, and the samples that showed the highest percentage of biodegradation were also analyzed via gas chromatography. Among the isolated actinobacteria, <jats:italic>Streptomyces aurantiogriseus<\/jats:italic> strain NORA7 (EMCC 28565) stood out for its ability to degrade crude oil (66.28\u2009\u00b1\u20096.25%). Gas chromatography revealed that docosane, nonadecane, pentacosane, and 7-methylpentadecane were the major compounds detected in the residual treated crude oil. Plackett\u2013Burman design (PB) was used to determine the critical factors impacting the biodegradation process. Response surface methodology (RSM) through Central Composite Design (CCD) was subsequently conducted, and the predicted optimum point of crude oil biodegradation was at 3% crude oil concentration, 0.15 g\/L yeast extract, and 25 mm inoculum size. The experimental value after optimum conditions was 70% after 3 weeks, which was close to the predicted value. A pot experiment was performed to investigate the outcomes of ex situ soil bioremediation, and the results were consistent with those of the flask-scale biodegradation experiment with enhanced removal of crude oil (92%). The results revealed that the ability of <jats:italic>S. aurantiogriseus<\/jats:italic> NORA7 to biodegrade crude oil could significantly contribute to the eco-friendly recovery of oil-polluted ecosystems and reduce the long-term environmental impact of crude oil pollution.<\/jats:p>\n          <jats:p>\n            <jats:bold>Graphical abstract<\/jats:bold>\n          <\/jats:p>","DOI":"10.1007\/s10532-025-10154-0","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T08:44:30Z","timestamp":1753433070000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Statistical optimization of crude oil bioremediation using Streptomyces aurantiogriseus isolated from Egypt\u2019s Western Desert"],"prefix":"10.1007","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5547-281X","authenticated-orcid":false,"given":"Sahar Y.","family":"Ibrahim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman A.","family":"Abdelhamid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5646-2325","authenticated-orcid":false,"given":"Ali M.","family":"El-Hagrassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1584-5730","authenticated-orcid":false,"given":"Noha M.","family":"Kamal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"issue":"8","key":"10154_CR1","doi-asserted-by":"publisher","first-page":"355","DOI":"10.21608\/ejchem.2022.111269.5063","volume":"65","author":"AS Abbas","year":"2022","unstructured":"Abbas AS, Refae RI, Amin GAE-F, Elsayed TR (2022) Characterization and potentials of microbial community in soil and water contaminated with crude oil in Egypt. Egypt J Chem 65(8):355\u2013370. https:\/\/doi.org\/10.21608\/ejchem.2022.111269.5063","journal-title":"Egypt J Chem"},{"issue":"1","key":"10154_CR2","doi-asserted-by":"publisher","first-page":"1116","DOI":"10.1038\/s41598-019-57224-x","volume":"10","author":"N Ali","year":"2020","unstructured":"Ali N, Dashti N, Khanafer M, Al-Awadhi H, Radwan S (2020) Bioremediation of soils saturated with spilled crude oil. Sci Rep 10(1):1116. https:\/\/doi.org\/10.1038\/s41598-019-57224-x","journal-title":"Sci Rep"},{"issue":"10","key":"10154_CR3","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.211003","volume":"8","author":"F Ali","year":"2021","unstructured":"Ali F, Das S, Hossain TJ, Chowdhury SI, Zedny SA, Das T et al (2021) Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites. R Soc Open Sci 8(10):211003. https:\/\/doi.org\/10.1098\/rsos.211003","journal-title":"R Soc Open Sci"},{"key":"10154_CR4","doi-asserted-by":"publisher","DOI":"10.1201\/9781420002980","volume-title":"Handbook of microbiological media for the examination of food","author":"RM Atlas","year":"2006","unstructured":"Atlas RM (2006) Handbook of microbiological media for the examination of food. CRC Press, Taylor & Francis Group LLC, New York"},{"key":"10154_CR5","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2025.178508","volume":"964","author":"C Baensch","year":"2025","unstructured":"Baensch C, Kr\u00f6ger A, Wilkes H (2025) Hydrocarbon biodegradation processes at a historic oil production site\u2014a signature metabolite study. Sci Total Environ 964:178508. https:\/\/doi.org\/10.1016\/j.scitotenv.2025.178508","journal-title":"Sci Total Environ"},{"issue":"2","key":"10154_CR6","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1007\/s40201-023-00868-9","volume":"21","author":"S Bajelani","year":"2023","unstructured":"Bajelani S, Enayatizamir N, Agha ABA, Sharifi R (2023) Potential of some microbial isolates on diesel hydrocarbons removal, bio surfactant production and biofilm formation. J Environ Health Sci Eng 21(2):417\u2013428. https:\/\/doi.org\/10.1007\/s40201-023-00868-9","journal-title":"J Environ Health Sci Eng"},{"issue":"3","key":"10154_CR7","first-page":"268","volume":"6","author":"F Baniasadi","year":"2009","unstructured":"Baniasadi F, Shahidi G, Karimi Nik A (2009) In vitro petroleum decomposition by actinomycetes isolated from petroleum contaminated soils. Am Eur J Agric Environ Sci 6(3):268\u2013270","journal-title":"Am Eur J Agric Environ Sci"},{"issue":"5","key":"10154_CR8","doi-asserted-by":"publisher","first-page":"2222","DOI":"10.1007\/s11368-019-02259-w","volume":"19","author":"H Baoune","year":"2019","unstructured":"Baoune H, Aparicio JD, Pucci G, Ould El Hadj-Khelil A, Polti MA (2019) Bioremediation of petroleum-contaminated soils using Streptomyces sp. Hlh1. J Soils Sed 19(5):2222\u20132230. https:\/\/doi.org\/10.1007\/s11368-019-02259-w","journal-title":"J Soils Sed"},{"issue":"1","key":"10154_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/MMBR.00019-15","volume":"80","author":"EA Barka","year":"2016","unstructured":"Barka EA, Vatsa P, Sanchez L, Gaveau-Vaillant N, Jacquard C, Klenk H-P et al (2016) Taxonomy, physiology, and natural products of actinobacteria. Microbiol Mol Biol Rev 80(1):1\u201343. https:\/\/doi.org\/10.1128\/MMBR.00019-15","journal-title":"Microbiol Mol Biol Rev"},{"key":"10154_CR10","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1016\/B978-0-444-63990-5.00016-5","volume-title":"Current developments in biotechnology and bioengineering","author":"JAV Costa","year":"2018","unstructured":"Costa JAV, Treichel H, Santos LO, Martins VG (2018) Solid-state fermentation for the production of biosurfactants and their applications. In: Pandey A, Larroche C, Soccol CR (eds) Current developments in biotechnology and bioengineering. Elsevier, Amsterdam, pp 357\u2013372"},{"key":"10154_CR11","volume-title":"Crude oil","author":"S Devanshi","year":"2021","unstructured":"Devanshi S, Shah KR, Arora S, Saxena S (2021) Actinomycetes as an environmental scrubber. In: Manar Elsayed A-R, Mohamed Hasan E-K (eds) Crude oil. IntechOpen, Rijeka"},{"issue":"2","key":"10154_CR12","doi-asserted-by":"publisher","first-page":"213","DOI":"10.4314\/jasem.v22i2.10","volume":"22","author":"KI Ehiosun","year":"2018","unstructured":"Ehiosun KI, Usman M (2018) Evaluation of crude oil biodegradation efficiency and peroxidase production by Streptomyces albus. J Appl Sci Environ Manage 22(2):213. https:\/\/doi.org\/10.4314\/jasem.v22i2.10","journal-title":"J Appl Sci Environ Manage"},{"issue":"3","key":"10154_CR13","doi-asserted-by":"publisher","first-page":"753","DOI":"10.21608\/ejbo.2016.2733","volume":"56","author":"AM El-Borai","year":"2016","unstructured":"El-Borai AM, Eltayeb KM, Mostafa AR, El-Assar SA (2016) Optimization and statistical evaluation of medium components affecting crude oil biodegradation by some locally isolated bacteria. Egypt J Bot 56(3):753\u2013767. https:\/\/doi.org\/10.21608\/ejbo.2016.2733","journal-title":"Egypt J Bot"},{"issue":"2","key":"10154_CR14","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1128\/am.11.2.75-77.1963","volume":"11","author":"MF El-Nakeeb","year":"1963","unstructured":"El-Nakeeb MF, Lechevalier HA, Lechevalier HA (1963) Selective isolation of aerobic actinomycetes. Appl Microbiol 11(2):75\u201377. https:\/\/doi.org\/10.1128\/am.11.2.75-77.1963","journal-title":"Appl Microbiol"},{"key":"10154_CR15","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2021.117556","volume":"286","author":"P Elumalai","year":"2021","unstructured":"Elumalai P, Parthipan P, AlSalhi MS, Huang M, Devanesan S, Karthikeyan OP et al (2021) Characterization of crude oil degrading bacterial communities and their impact on biofilm formation. Environ Pollut 286:117556. https:\/\/doi.org\/10.1016\/j.envpol.2021.117556","journal-title":"Environ Pollut"},{"key":"10154_CR16","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.137258","volume":"722","author":"DK Ferguson","year":"2020","unstructured":"Ferguson DK, Li C, Jiang C, Chakraborty A, Grasby SE, Hubert CRJ (2020) Natural attenuation of spilled crude oil by cold-adapted soil bacterial communities at a decommissioned High Arctic oil well site. Sci Total Environ 722:137258. https:\/\/doi.org\/10.1016\/j.scitotenv.2020.137258","journal-title":"Sci Total Environ"},{"key":"10154_CR17","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1016\/j.ibiod.2013.10.003","volume":"86","author":"FZ Ferradji","year":"2014","unstructured":"Ferradji FZ, Mnif S, Badis A, Rebbani S, Fodil D, Eddouaouda K, Sayadi S (2014) Naphthalene and crude oil degradation by biosurfactant producing Streptomyces spp. isolated from Mitidja plain soil (North of Algeria). Int Biodeterior Biodegrad 86:300\u2013308. https:\/\/doi.org\/10.1016\/j.ibiod.2013.10.003","journal-title":"Int Biodeterior Biodegrad"},{"key":"10154_CR18","doi-asserted-by":"publisher","DOI":"10.3390\/fermentation9010021","author":"TF Ferreira","year":"2023","unstructured":"Ferreira TF, Martins FF, Cayres CA, Amaral PFF, Azevedo DD, Coelho MA (2023) Biosurfactant production from the biodegradation of n-paraffins, isoprenoids and aromatic hydrocarbons from crude petroleum by Yarrowia lipolytica IMUFRJ 50682. Fermentation. https:\/\/doi.org\/10.3390\/fermentation9010021","journal-title":"Fermentation"},{"key":"10154_CR19","first-page":"59","volume":"4","author":"UM George","year":"2011","unstructured":"George UM, Chandraja CV, Immanuel G, Chandran R (2011) Petroleum degrading activity of actinomycetes isolated from the coastal areas of Tamilnadu. Int J Agric Environ Biotechnol 4:59\u201365","journal-title":"Int J Agric Environ Biotechnol"},{"key":"10154_CR20","doi-asserted-by":"publisher","DOI":"10.1002\/9781118434635.ch01","author":"D Granato","year":"2014","unstructured":"Granato D, de Ara\u00fajo Calado VM (2014) The use and importance of design of experiments (DOE) in process modelling in food science and technology. Math Stat Methods Food Sci Technol. https:\/\/doi.org\/10.1002\/9781118434635.ch01","journal-title":"Math Stat Methods Food Sci Technol"},{"issue":"6","key":"10154_CR21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0131299","volume":"10","author":"DG Groenendyk","year":"2015","unstructured":"Groenendyk DG, Ferr\u00e9 TPA, Thorp KR, Rice AK (2015) Hydrologic-process-based soil texture classifications for improved visualization of landscape function. PLoS ONE 10(6):e0131299. https:\/\/doi.org\/10.1371\/journal.pone.0131299","journal-title":"PLoS ONE"},{"issue":"7","key":"10154_CR22","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1016\/j.chemosphere.2005.03.066","volume":"61","author":"MO Ilori","year":"2005","unstructured":"Ilori MO, Amobi CJ, Odocha AC (2005) Factors affecting biosurfactant production by oil degrading Aeromonas spp. isolated from a tropical environment. Chemosphere 61(7):985\u2013992. https:\/\/doi.org\/10.1016\/j.chemosphere.2005.03.066","journal-title":"Chemosphere"},{"issue":"2","key":"10154_CR23","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1016\/0922-338X(95)94092-6","volume":"79","author":"K Iwahori","year":"1995","unstructured":"Iwahori K, Wang M, Taki H, Fujita M (1995) Comparative studies on utilization of fatty acids and hydrocarbons in Nocardia amarae and Rhodococcus spp. J Ferment Bioeng 79(2):186\u2013189. https:\/\/doi.org\/10.1016\/0922-338X(95)94092-6","journal-title":"J Ferment Bioeng"},{"key":"10154_CR24","doi-asserted-by":"publisher","first-page":"880","DOI":"10.3389\/fmicb.2015.00880","volume":"6","author":"R Jarling","year":"2015","unstructured":"Jarling R, K\u00fchner S, Bas\u00edlio Janke E, Gruner A, Drozdowska M, Golding BT et al (2015) Versatile transformations of hydrocarbons in anaerobic bacteria: substrate ranges and regio- and stereo-chemistry of activation reactions. Front Microbiol 6:880. https:\/\/doi.org\/10.3389\/fmicb.2015.00880","journal-title":"Front Microbiol"},{"issue":"3","key":"10154_CR25","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.petlm.2018.03.007","volume":"4","author":"AP Karlapudi","year":"2018","unstructured":"Karlapudi AP, Venkateswarulu TC, Tammineedi J, Kanumuri L, Ravuru BK, Dirisala Vr, Kodali VP (2018) Role of biosurfactants in bioremediation of oil pollution-a review. Petroleum 4(3):241\u2013249. https:\/\/doi.org\/10.1016\/j.petlm.2018.03.007","journal-title":"Petroleum"},{"key":"10154_CR26","doi-asserted-by":"publisher","DOI":"10.3389\/fenvs.2021.794303","author":"A Khandelwal","year":"2022","unstructured":"Khandelwal A, Sugavanam R, Ramakrishnan B, Dutta A, Varghese E, Nain L et al (2022) Free and immobilized microbial culture-mediated crude oil degradation and microbial diversity changes through taxonomic and functional markers in a sandy loam soil. Front Environ Sci. https:\/\/doi.org\/10.3389\/fenvs.2021.794303","journal-title":"Front Environ Sci"},{"issue":"S1","key":"10154_CR27","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1002\/vjch.202300297","volume":"62","author":"TQH Kieu","year":"2024","unstructured":"Kieu TQH, Nguyen TY, Nguyen VT, Nguyen TNQ (2024) Biosurfactant production by a crude oil-utilizing bacterium towards petroleum hydrocarbon biodegradation applications. Vietnam J Chem 62(S1):33\u201339. https:\/\/doi.org\/10.1002\/vjch.202300297","journal-title":"Vietnam J Chem"},{"issue":"11","key":"10154_CR28","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1007\/s11270-015-2649-2","volume":"226","author":"A Klamerus-Iwan","year":"2015","unstructured":"Klamerus-Iwan A, B\u0142o\u0144ska E, Lasota J, Kalandyk A, Walig\u00f3rski P (2015) Influence of oil contamination on physical and biological properties of forest soil after chainsaw use. Water Air Soil Pollut 226(11):389. https:\/\/doi.org\/10.1007\/s11270-015-2649-2","journal-title":"Water Air Soil Pollut"},{"key":"10154_CR29","doi-asserted-by":"publisher","first-page":"1487","DOI":"10.1007\/978-3-031-09710-2_65","volume-title":"Handbook of biodegradable materials","author":"LM Koh","year":"2023","unstructured":"Koh LM, Khor SM (2023) Biodegradation of crude oil and biodegradation of surfactants. In: Ali GAM, Makhlouf ASH (eds) Handbook of biodegradable materials. Springer, Cham, pp 1487\u20131531"},{"issue":"2","key":"10154_CR30","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/j.aoas.2015.09.001","volume":"60","author":"AS Korayem","year":"2015","unstructured":"Korayem AS, Abdelhafez AA, Zaki MM, Saleh EA (2015) Optimization of biosurfactant production by Streptomyces isolated from Egyptian arid soil using Plackett-Burman design. Ann Agric Sci 60(2):209\u2013217. https:\/\/doi.org\/10.1016\/j.aoas.2015.09.001","journal-title":"Ann Agric Sci"},{"issue":"1","key":"10154_CR31","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1046\/j.1365-2958.2003.03751.x","volume":"51","author":"I Kuiper","year":"2004","unstructured":"Kuiper I, Lagendijk EL, Pickford R, Derrick JP, Lamers GEM, Thomas-Oates JE et al (2004) Characterization of two Pseudomonas putida lipopeptide biosurfactants, putisolvin I and II, which inhibit biofilm formation and break down existing biofilms. Mol Microbiol 51(1):97\u2013113. https:\/\/doi.org\/10.1046\/j.1365-2958.2003.03751.x","journal-title":"Mol Microbiol"},{"key":"10154_CR32","doi-asserted-by":"publisher","DOI":"10.3390\/pr10112256","author":"H Lee","year":"2022","unstructured":"Lee H, Kim D-U (2022) Biodegradation of alachlor by a newly isolated bacterium: degradation pathway and product analysis. Processes. https:\/\/doi.org\/10.3390\/pr10112256","journal-title":"Processes"},{"key":"10154_CR33","volume-title":"Brock biology of microorganisms","author":"MT Madigan","year":"1997","unstructured":"Madigan MT, Martinko JM, Parker J, Brock TD (1997) Brock biology of microorganisms. Prentice-Hall International Inc, New Jersey"},{"issue":"3","key":"10154_CR34","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1023\/A:1010392821353","volume":"128","author":"FJ M\u00e1rquez-Rocha","year":"2001","unstructured":"M\u00e1rquez-Rocha FJ, Hern\u00e1ndez-Rodr\u00ed V, Lamela MT (2001) Biodegradation of diesel oil in soil by a microbial consortium. Water Air Soil Pollut 128(3):313\u2013320. https:\/\/doi.org\/10.1023\/A:1010392821353","journal-title":"Water Air Soil Pollut"},{"issue":"1","key":"10154_CR35","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1007\/s44371-024-00038-2","volume":"1","author":"MI Matilda","year":"2024","unstructured":"Matilda MI, Samuel HS (2024) Bioremediation of oil spill: concept, methods and applications. Discover Chem 1(1):42. https:\/\/doi.org\/10.1007\/s44371-024-00038-2","journal-title":"Discover Chem"},{"key":"10154_CR36","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/328608","volume":"2013","author":"S Mohanty","year":"2013","unstructured":"Mohanty S, Jasmine J, Mukherji S (2013) Practical considerations and challenges involved in surfactant enhanced bioremediation of oil. BioMed Res Int 2013:328608. https:\/\/doi.org\/10.1155\/2013\/328608","journal-title":"BioMed Res Int"},{"issue":"4","key":"10154_CR37","first-page":"113","volume":"9","author":"IA Mona Othman","year":"2020","unstructured":"Mona Othman IA (2020) Isolation, characterization and molecular identification of oil degrading Streptomyces sp. MB9 from soils. Int J Pharm Res Allied Sci 9(4):113\u2013124","journal-title":"Int J Pharm Res Allied Sci"},{"issue":"3","key":"10154_CR38","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1111\/j.1574-6941.2002.tb01031.x","volume":"42","author":"P Monciardini","year":"2002","unstructured":"Monciardini P, Sosio M, Cavaletti L, Chiocchini C, Donadio S (2002) New PCR primers for the selective amplification of 16S rDNA from different groups of actinomycetes. FEMS Microbiol Ecol 42(3):419\u2013429. https:\/\/doi.org\/10.1111\/j.1574-6941.2002.tb01031.x","journal-title":"FEMS Microbiol Ecol"},{"key":"10154_CR39","doi-asserted-by":"publisher","DOI":"10.1086\/383067","volume-title":"Manual of clinical microbiology","author":"PR Murray","year":"2003","unstructured":"Murray PR (2003) Manual of clinical microbiology. American Society for Microbiology Press, Washington, D.C. https:\/\/doi.org\/10.1086\/383067"},{"issue":"10","key":"10154_CR40","doi-asserted-by":"publisher","first-page":"1522","DOI":"10.1016\/j.jiph.2020.06.025","volume":"13","author":"P Muthukrishnan","year":"2020","unstructured":"Muthukrishnan P, Chithra Devi D, Mostafa AA, Alsamhary KI, Abdel-Raouf N, Nageh Sholkamy E (2020) Antimicrobial efficacy of Nocardiopsis sp. MK_MSt033 against selected multidrug resistant clinical microbial pathogens. J Infect Public Health 13(10):1522\u20131532. https:\/\/doi.org\/10.1016\/j.jiph.2020.06.025","journal-title":"J Infect Public Health"},{"key":"10154_CR41","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2021.133168","volume":"289","author":"B Muthukumar","year":"2022","unstructured":"Muthukumar B, Parthipan P, AlSalhi MS, Prabhu NS, Rao TN, Devanesan S et al (2022) Characterization of bacterial community in oil-contaminated soil and its biodegradation efficiency of high molecular weight (>C40) hydrocarbon. Chemosphere 289:133168. https:\/\/doi.org\/10.1016\/j.chemosphere.2021.133168","journal-title":"Chemosphere"},{"key":"10154_CR42","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2022.136826","volume":"310","author":"B Muthukumar","year":"2023","unstructured":"Muthukumar B, Surya S, Sivakumar K, AlSalhi MS, Rao TN, Devanesan S et al (2023) Influence of bioaugmentation in crude oil contaminated soil by Pseudomonas species on the removal of total petroleum hydrocarbon. Chemosphere 310:136826. https:\/\/doi.org\/10.1016\/j.chemosphere.2022.136826","journal-title":"Chemosphere"},{"key":"10154_CR43","doi-asserted-by":"publisher","DOI":"10.3390\/fermentation9040369","author":"PV Nagime","year":"2023","unstructured":"Nagime PV, Upaichit A, Cheirsilp B, Boonsawang P (2023a) Bio-succinic acid production from palm oil mill effluent using Enterococcus gallinarum with sequential purification of biogas. Fermentation. https:\/\/doi.org\/10.3390\/fermentation9040369","journal-title":"Fermentation"},{"issue":"3","key":"10154_CR44","doi-asserted-by":"publisher","first-page":"1149","DOI":"10.1002\/bab.2428","volume":"70","author":"PV Nagime","year":"2023","unstructured":"Nagime PV, Upaichit A, Cheirsilp B, Boonsawang P (2023b) Isolation and screening of microorganisms for high yield of succinic acid production. Biotechnol Appl Biochem 70(3):1149\u20131161. https:\/\/doi.org\/10.1002\/bab.2428","journal-title":"Biotechnol Appl Biochem"},{"issue":"1","key":"10154_CR45","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/S0304-3894(03)00065-7","volume":"100","author":"G Nano","year":"2003","unstructured":"Nano G, Borroni A, Rota R (2003) Combined slurry and solid-phase bioremediation of diesel contaminated soils. J Hazard Mater 100(1):79\u201394. https:\/\/doi.org\/10.1016\/S0304-3894(03)00065-7","journal-title":"J Hazard Mater"},{"key":"10154_CR46","unstructured":"NCBI (2024a) PubChem compound summary for CID 12534, tricosane. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR47","unstructured":"NCBI (2024b) PubChem compound summary for CID 86541, 4,5-dimethylnonane. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR48","unstructured":"NCBI (2024c) PubChem compound summary for CID 5284421, methyl linoleate. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/Methyl-linoleate"},{"key":"10154_CR49","unstructured":"NCBI (2024d) PubChem compound summary for CID 17152, decyl ether. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/Decyl-ether"},{"key":"10154_CR50","unstructured":"NCBI (2024e) PubChem compound summary for CID 1045, 1,4-diaminobutane. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/1_4-Diaminobutane"},{"key":"10154_CR51","unstructured":"NCBI (2024f) PubChem compound summary for CID 75050, 2-(octadecyloxy)ethanol. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/2-_Octadecyloxy_ethanol"},{"key":"10154_CR52","unstructured":"NCBI (2024g) PubChem compound summary for CID 14900, 2,3-dihydroxypropyl hexadecanoate. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/2_3-Dihydroxypropyl-hexadecanoate"},{"key":"10154_CR53","unstructured":"NCBI (2024h) PubChem compound summary for CID 15267, 2-methylpentadecane. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/2-Methylpentadecane"},{"key":"10154_CR54","unstructured":"NCBI (2024i) PubChem compound summary for CID 13534, 3,4-dimethylheptane. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethylheptane"},{"key":"10154_CR55","unstructured":"NCBI (2024j) PubChem compound summary for CID 85926, 3,4-dimethyloctane. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR56","unstructured":"NCBI (2024k) PubChem compound summary for CID 134601, aspartame. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR57","unstructured":"NCBI (2024l) PubChem compound summary for CID 18016, dibzutyl azelate. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR58","unstructured":"NCBI (2024m) PubChem compound summary for CID 8222, eicosane. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR59","unstructured":"NCBI (2024n) PubChem compound summary for CID 12398, heptadecane. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR60","unstructured":"NCBI (2024o) PubChem compound summary for CID 12401, nonadecane. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR61","unstructured":"NCBI (2024p) PubChem compound summary for CID 11635, octadecane. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR62","unstructured":"NCBI (2024q) PubChem compound summary for CID 5281, stearic acid. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR63","unstructured":"NCBI (2024r) PubChem compound summary for CID 165580, paromomycin. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR64","unstructured":"NCBI (2024s) PubChem compound summary for CID 12391, pentadecane. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR65","unstructured":"NCBI (2024t) PubChem compound summary for CID 12389, tetradecane. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"key":"10154_CR66","unstructured":"NCBI (2024u) PubChem compound summary for CID 8207, 1-tridecanol. https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/3_4-Dimethyloctane"},{"issue":"16","key":"10154_CR67","doi-asserted-by":"publisher","first-page":"19451","DOI":"10.1007\/s13399-023-04115-x","volume":"14","author":"H Noveiri","year":"2024","unstructured":"Noveiri H, Nozari M, Almasi H, Fard NJH, Jorfi S, Takdastan A, Montazeri EA (2024) Biodegradation of N-hexadecane using bacterial consortium isolated from seawater contaminated with petroleum. Biomass Conv Biorefinery 14(16):19451\u201319460. https:\/\/doi.org\/10.1007\/s13399-023-04115-x","journal-title":"Biomass Conv Biorefinery"},{"issue":"2","key":"10154_CR68","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1080\/10889868.2015.1113926","volume":"20","author":"FM Olajuyigbe","year":"2016","unstructured":"Olajuyigbe FM, Ehiosun KI (2016) Assessment of crude oil degradation efficiency of newly isolated actinobacteria reveals untapped bioremediation potentials. Bioremed J 20(2):133\u2013143. https:\/\/doi.org\/10.1080\/10889868.2015.1113926","journal-title":"Bioremed J"},{"key":"10154_CR69","doi-asserted-by":"publisher","DOI":"10.3390\/toxics10080417","author":"TA Olasehinde","year":"2022","unstructured":"Olasehinde TA, Olaniran AO (2022) Neurotoxicity of polycyclic aromatic hydrocarbons: a systematic mapping and review of neuropathological mechanisms. Toxics. https:\/\/doi.org\/10.3390\/toxics10080417","journal-title":"Toxics"},{"issue":"4","key":"10154_CR70","doi-asserted-by":"publisher","first-page":"2224","DOI":"10.20546\/ijcmas.2018.704.253","volume":"7","author":"DR Parikh","year":"2018","unstructured":"Parikh DR, Tipre DR, Nayak NS, Dave SR (2018) Degradation of discarded used engine oil by Pseudomonas aeruginosa DP-1 and its optimization. Int J Curr Microbiol Appl Sci 7(4):2224\u20132229. https:\/\/doi.org\/10.20546\/ijcmas.2018.704.253","journal-title":"Int J Curr Microbiol Appl Sci"},{"issue":"4","key":"10154_CR71","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1093\/biomet\/33.4.305","volume":"33","author":"RL Plackett","year":"1946","unstructured":"Plackett RL, Burman JP (1946) The design of optimum multifactorial experiments. Biometrika 33(4):305\u2013325. https:\/\/doi.org\/10.1093\/biomet\/33.4.305","journal-title":"Biometrika"},{"issue":"1","key":"10154_CR72","first-page":"87","volume":"17","author":"GA P\u0142aza","year":"2008","unstructured":"P\u0142aza GA, \u0141ukasik K, Wypych J, Na\u0142\u0119cz-Jawecki G, Berry C, Brigmon RL (2008) Biodegradation of crude oil and distillation products by biosurfactant-producing bacteria. Pol J Environ Stud 17(1):87\u201394","journal-title":"Pol J Environ Stud"},{"key":"10154_CR73","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1016\/j.sajce.2021.06.004","volume":"37","author":"LT Popoola","year":"2021","unstructured":"Popoola LT, Yusuff AS (2021) Optimization and characterization of crude oil contaminated soil bioremediation using bacteria isolates: plant growth effect. S Afr J Chem Eng 37:206\u2013213. https:\/\/doi.org\/10.1016\/j.sajce.2021.06.004","journal-title":"S Afr J Chem Eng"},{"key":"10154_CR74","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.sajce.2021.10.003","volume":"39","author":"LT Popoola","year":"2022","unstructured":"Popoola LT, Yusuff AS, Adeyi AA, Omotara OO (2022) Bioaugmentation and biostimulation of crude oil contaminated soil: process parameters influence. S Afr J Chem Eng 39:12\u201318. https:\/\/doi.org\/10.1016\/j.sajce.2021.10.003","journal-title":"S Afr J Chem Eng"},{"issue":"3","key":"10154_CR75","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1128\/jb.81.3.431-441.1961","volume":"81","author":"TG Pridham","year":"1961","unstructured":"Pridham TG, Lyons AJ Jr (1961) Streptomyces albus (Rossi-Doria) Waksman et Henrici: taxonomic study of strains labeled Streptomyces albus. J Bacteriol 81(3):431\u2013441. https:\/\/doi.org\/10.1128\/jb.81.3.431-441.1961","journal-title":"J Bacteriol"},{"issue":"1\u20133","key":"10154_CR76","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1159\/000443997","volume":"26","author":"R Rabus","year":"2016","unstructured":"Rabus R, Boll M, Heider J, Meckenstock RU, Buckel W, Einsle O et al (2016) Anaerobic microbial degradation of hydrocarbons: from enzymatic reactions to the environment. J Mol Microbiol Biotechnol 26(1\u20133):5\u201328. https:\/\/doi.org\/10.1159\/000443997","journal-title":"J Mol Microbiol Biotechnol"},{"key":"10154_CR77","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.7389","volume":"7","author":"K Robichaud","year":"2019","unstructured":"Robichaud K, Lebeau M, Martineau S, Amyot M (2019) Bioremediation of engine-oil contaminated soil using local residual organic matter. PeerJ 7:e7389. https:\/\/doi.org\/10.7717\/peerj.7389","journal-title":"PeerJ"},{"key":"10154_CR78","doi-asserted-by":"publisher","DOI":"10.3390\/agriculture14101758","author":"A S\u00e1h\u00f3","year":"2024","unstructured":"S\u00e1h\u00f3 A, Karik\u00e1s V, \u00c1sv\u00e1nyi B, Lakatos E, Varga L, Greff B (2024) Bioactive potential of actinobacteria strains isolated from the rhizosphere of lavender, lemon balm, and oregano. Agriculture. https:\/\/doi.org\/10.3390\/agriculture14101758","journal-title":"Agriculture"},{"key":"10154_CR79","volume-title":"Molecular cloning: a laboratory manual","author":"J Sambrook","year":"1989","unstructured":"Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York"},{"issue":"3","key":"10154_CR80","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1099\/00207713-16-3-313","volume":"16","author":"EB Shirling","year":"1966","unstructured":"Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16(3):313\u2013340. https:\/\/doi.org\/10.1099\/00207713-16-3-313","journal-title":"Int J Syst Bacteriol"},{"issue":"2","key":"10154_CR81","doi-asserted-by":"publisher","first-page":"633","DOI":"10.12944\/CRNFSJ.10.2.19","volume":"10","author":"N Singhania","year":"2022","unstructured":"Singhania N, Barmanray A (2022) Optimization of phenolic compounds extraction from wood apple (Limonia acidissima L.) fruit using experimental design methodology. Curr Res Nutr Food Sci 10(2):633\u2013646. https:\/\/doi.org\/10.12944\/CRNFSJ.10.2.19","journal-title":"Curr Res Nutr Food Sci"},{"key":"10154_CR82","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1007\/978-3-030-68031-2_29","volume-title":"Waste recycling technologies for nanomaterials manufacturing","author":"FS Soliman","year":"2021","unstructured":"Soliman FS, El-Maghrabi HH, Ali GAM, Kammoun MA, Nada AA (2021) Reinforcement of petroleum wax by-product paraffins as phase change materials for thermal energy storage by recycled nanomaterials. In: Makhlouf ASH, Ali GAM (eds) Waste recycling technologies for nanomaterials manufacturing. Springer, Cham, pp 823\u2013850. https:\/\/doi.org\/10.1007\/978-3-030-68031-2_29"},{"key":"10154_CR83","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jbiotec.2022.03.006","volume":"349","author":"S Soumeya","year":"2022","unstructured":"Soumeya S, Allaoueddine B, Hocine A-K (2022) Biodegradation of used motor oil by Streptomyces ginkgonis KM-1\u20132, isolated from soil polluted by waste oils in the region of Azzaba (Skikda-Algeria). J Biotechnol 349:1\u201311. https:\/\/doi.org\/10.1016\/j.jbiotec.2022.03.006","journal-title":"J Biotechnol"},{"key":"10154_CR84","doi-asserted-by":"publisher","DOI":"10.1007\/s12088-024-01222-9","author":"M Srimathi","year":"2024","unstructured":"Srimathi M, Suganthi M, Sugitha S, Ashok Kumar K, Ramprasath C, Abirami G (2024) Characterization of crude oil degrading marine bacterium Bacillus licheniformis. Indian J Microbiol. https:\/\/doi.org\/10.1007\/s12088-024-01222-9","journal-title":"Indian J Microbiol"},{"key":"10154_CR85","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2025.121085","volume":"271","author":"M Tahmasbizadeh","year":"2025","unstructured":"Tahmasbizadeh M, Nikaeen M, Movahedian Attar H, Khanahmad H, Khodadadi M (2025) Resuscitation-promoting factors: novel strategies for the bioremediation of crude oil-contaminated soils. Environ Res 271:121085. https:\/\/doi.org\/10.1016\/j.envres.2025.121085","journal-title":"Environ Res"},{"key":"10154_CR86","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1093\/molbev\/mst197","volume":"10","author":"K Tamura","year":"1993","unstructured":"Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512\u2013526. https:\/\/doi.org\/10.1093\/molbev\/mst197","journal-title":"Mol Biol Evol"},{"issue":"22","key":"10154_CR87","doi-asserted-by":"publisher","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","volume":"22","author":"JD Thompson","year":"1994","unstructured":"Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22(22):4673\u20134680. https:\/\/doi.org\/10.1093\/nar\/22.22.4673","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"10154_CR88","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1007\/s11274-004-5958-y","volume":"21","author":"T Tugrul","year":"2005","unstructured":"Tugrul T, Cansunar E (2005) Detecting surfactant-producing microorganisms by the drop-collapse test. World J Microbiol Biotechnol 21(6):851\u2013853. https:\/\/doi.org\/10.1007\/s11274-004-5958-y","journal-title":"World J Microbiol Biotechnol"},{"key":"10154_CR89","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.141043","volume":"745","author":"S Varjani","year":"2020","unstructured":"Varjani S, Pandey A, Upasani VN (2020) Oilfield waste treatment using novel hydrocarbon utilizing bacterial consortium\u2014a microcosm approach. Sci Total Environ 745:141043. https:\/\/doi.org\/10.1016\/j.scitotenv.2020.141043","journal-title":"Sci Total Environ"},{"key":"10154_CR90","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/B978-0-12-819025-8.00020-X","volume-title":"Bioremediation of pollutants","author":"GS Vishwakarma","year":"2020","unstructured":"Vishwakarma GS, Bhattacharjee G, Gohil N, Singh V (2020) Current status, challenges and future of bioremediation. In: Pandey VC, Singh V (eds) Bioremediation of pollutants. Elsevier, Amsterdam, pp 403\u2013415"},{"key":"10154_CR91","volume-title":"Bergey\u2019s manual of systematic bacteriology","author":"WB Whitman","year":"2012","unstructured":"Whitman WB (2012) The actinobacteria. In: Goodfellow M, K\u00e4mpfer P, Busse H-J, Trujillo ME, Suzuki K-I, Ludwig W, Whitman WB (eds) Bergey\u2019s manual of systematic bacteriology. Springer, New York"},{"key":"10154_CR92","volume-title":"Actinomycete systematics-principle, methods and practice","author":"LH Xu","year":"2007","unstructured":"Xu LH, Li WJ, Liu ZH, Jiang CL (2007) Actinomycete systematics-principle, methods and practice. Science Press, Beijing"}],"container-title":["Biodegradation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10532-025-10154-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10532-025-10154-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10532-025-10154-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T23:13:25Z","timestamp":1757286805000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10532-025-10154-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,25]]},"references-count":92,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10154"],"URL":"https:\/\/doi.org\/10.1007\/s10532-025-10154-0","relation":{},"ISSN":["0923-9820","1572-9729"],"issn-type":[{"value":"0923-9820","type":"print"},{"value":"1572-9729","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,25]]},"assertion":[{"value":"28 April 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors have declared no competing interests that are relevant to the content of this article.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Not applicable.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to publish"}}],"article-number":"68"}}