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In this study we analysed the antimicrobial activity of two chelators, specifically 3-hydroxy-1,2-dimethyl-4(1H)-pyridone (deferiprone, DFP), which is clinically approved for the treatment of iron overload disorders, and its 1,2-diethyl homologue, CP94. Both compounds showed moderate activity towards planktonically growing\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    cells, and the mechanism of action of these chelators was indeed by limiting the amount of free iron. Surprisingly, the compounds behaved very differently when the cells were grown in biofilms. DFP also showed inhibitory effects on biofilm formation but in contrast, CP94 stimulated this process, in particular at high concentrations. We hypothesised that CP94 behaves as an iron carrier, which was confirmed by our observation that it had antimicrobial synergy with the toxic metals, gallium and copper. This suggests that\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    produces a biofilm-specific transport protein that recognises CP94 but not the closely related compound DFP.\n                  <\/jats:p>","DOI":"10.1007\/s10534-020-00281-x","type":"journal-article","created":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T09:21:50Z","timestamp":1610356910000},"page":"315-328","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Biofilm-specific uptake of a 4-pyridone-based iron chelator by Pseudomonas aeruginosa"],"prefix":"10.1007","volume":"34","author":[{"given":"Sharareh","family":"Houshmandyar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0569-0082","authenticated-orcid":false,"given":"Ian M.","family":"Eggleston","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9307-0515","authenticated-orcid":false,"given":"Albert","family":"Bolhuis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,11]]},"reference":[{"key":"281_CR1","doi-asserted-by":"publisher","first-page":"1415","DOI":"10.1128\/JB.181.5.1415-1428.1999","volume":"181","author":"H Abdul-Tehrani","year":"1999","unstructured":"Abdul-Tehrani H, Hudson AJ, Chang YS, Timms AR, Hawkins C, Williams JM, Harrison PM, Guest JR, Andrews SC (1999) Ferritin mutants of Escherichia coli are iron deficient and growth impaired, and fur mutants are iron deficient. 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