{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T20:43:16Z","timestamp":1781296996928,"version":"3.54.1"},"reference-count":76,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"MIT Presidential Fellowship"},{"name":"Robert and Ardis James Foundation"},{"DOI":"10.13039\/100019235","name":"Abdul Latif Jameel Water and Food Systems Lab, Massachusetts Institute of Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019235","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R35GM157324-01"],"award-info":[{"award-number":["1R35GM157324-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006919","name":"Massachusetts Institute of Technology","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100006919","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biometals"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The macronutrient phosphorus is vital for sustaining cellular processes in all life forms. Due to its frequent adsorption on iron minerals, phosphorus bioavailability is low in many soils. While the abiotic adsorption of phosphate on iron minerals has been well studied, the direct effects of this process on bioavailability to plants and microbes has not been thoroughly investigated in a simplified laboratory system. We developed a hydroponic growth system that uses hydrous ferric oxide (HFO) to induce phosphorus limitation and can enable both plant and microbial cultivation as well as gnotobiotic co-culture. We demonstrate that this system can be used for phosphorus-limited growth of the model plant\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    as well as two root-associated bacterial isolates (from the genera\n                    <jats:italic>Rhizobium<\/jats:italic>\n                    and\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    ). Elemental analysis of phosphorus and iron concentration in\n                    <jats:italic>A. thaliana<\/jats:italic>\n                    shoots reveals that the addition of increasing amounts of HFO leads to a progressive decrease in phosphorus concentration but does not affect iron quotas. We also report that phosphorus concentrations in both bacterial isolates decrease when cultivated in media supplemented with HFO. We further show that\n                    <jats:italic>A. thaliana<\/jats:italic>\n                    can be co-cultured with a\u00a0\n                    <jats:italic>Rhizobium<\/jats:italic>\n                    isolate\u00a0in our phosphorus-limited hydroponic system with bacteria relying on plant photosynthate as their sole carbon source. Our work provides a controlled demonstration of the effects of mineral adsorption on phosphorus bioavailability and a tool for further investigation of how plants and microbes access phosphorus in the environment.\n                  <\/jats:p>","DOI":"10.1007\/s10534-025-00767-6","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T08:12:50Z","timestamp":1764231170000},"page":"359-373","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Gnotobiotic growth and phosphorus limitation of Arabidopsis thaliana and co-occurring microbes on phosphated iron oxides"],"prefix":"10.1007","volume":"39","author":[{"given":"Amanda M.","family":"Mackie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Schuler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darcy L.","family":"McRose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"767_CR1","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1016\/j.gca.2014.06.024","volume":"141","author":"MM Akafia","year":"2014","unstructured":"Akafia MM, Harrington JM, Bargar JR, Duckworth OW (2014) Metal oxyhydroxide dissolution as promoted by structurally diverse siderophores and oxalate. Geochim Cosmochim Acta 141:258\u2013269. https:\/\/doi.org\/10.1016\/j.gca.2014.06.024","journal-title":"Geochim Cosmochim Acta"},{"issue":"7582","key":"767_CR2","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1038\/nature16192","volume":"528","author":"Y Bai","year":"2015","unstructured":"Bai Y, M\u00fcller DB, Srinivas G et al (2015) Functional overlap of the Arabidopsis leaf and root microbiota. Nature 528(7582):364\u2013369. https:\/\/doi.org\/10.1038\/nature16192","journal-title":"Nature"},{"issue":"6","key":"767_CR3","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1007\/s10534-020-00248-y","volume":"33","author":"M Baune","year":"2020","unstructured":"Baune M, Kang K, Schenkeveld WDC, Kraemer SM, Hayen H, Weber G (2020) Importance of oxidation products in coumarin-mediated Fe(Hydr)oxide mineral dissolution. Biometals: Int J Role Metal Ions Biol Biochem Med 33(6):305\u2013321. https:\/\/doi.org\/10.1007\/s10534-020-00248-y","journal-title":"Biometals: Int J Role Metal Ions Biol Biochem Med"},{"key":"767_CR4","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00548","author":"A Becquer","year":"2014","unstructured":"Becquer A, Trap J, Irshad U, Ali MA, Claude P (2014) From soil to plant, the journey of P through trophic relationships and ectomycorrhizal association. Front Plant Sci. https:\/\/doi.org\/10.3389\/fpls.2014.00548","journal-title":"Front Plant Sci"},{"key":"767_CR5","unstructured":"Bhattacharjee A, Aufrecht J, Richardson J et al. (2024) TerraForms User Program- Capability Development, User Science, Collaborations, and Deployment. Paper presented at Enabling Capabilities and Resources 2024 Principal Invesitgator Meetings Proceedings. April."},{"key":"767_CR6","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00580-20","author":"RM Boiteau","year":"2021","unstructured":"Boiteau RM, Markillie LM, Hoyt DW et al (2021) Metabolic interactions between Brachypodium and Pseudomonas fluorescens under controlled iron-limited conditions. mSystems. https:\/\/doi.org\/10.1128\/msystems.00580-20","journal-title":"mSystems"},{"key":"767_CR7","unstructured":"Borch T, Fendorf S (2008) Phosphate Interactions with Iron (Hydr)Oxides: Mineralization Pathways and Phosphorus Retention Upon Bioreduction. MO Barnett and DB Kent; Elsevier, Amsterdam, Netherlands. https:\/\/www.osti.gov\/biblio\/959208."},{"issue":"24","key":"767_CR8","doi-asserted-by":"publisher","first-page":"14155","DOI":"10.1021\/acs.est.7b03878","volume":"51","author":"KE Brodersen","year":"2017","unstructured":"Brodersen KE, Koren K, Mo\u00dfhammer M, Ralph PJ, K\u00fchl M, Santner J (2017) Seagrass-mediated phosphorus and iron solubilization in tropical sediments. Environ Sci Technol 51(24):14155\u201314163. https:\/\/doi.org\/10.1021\/acs.est.7b03878","journal-title":"Environ Sci Technol"},{"issue":"2","key":"767_CR9","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1038\/s43016-021-00232-w","volume":"2","author":"WJ Brownlie","year":"2021","unstructured":"Brownlie WJ, Sutton MA, Reay DS et al (2021) Global actions for a sustainable phosphorus future. Nat Food 2(2):71\u201374. https:\/\/doi.org\/10.1038\/s43016-021-00232-w","journal-title":"Nat Food"},{"issue":"1","key":"767_CR10","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1104\/pp.17.01624","volume":"177","author":"A Carstensen","year":"2018","unstructured":"Carstensen A, Herdean A, Schmidt SB et al (2018) The impacts of phosphorus deficiency on the photosynthetic electron transport chain. Plant Physiol 177(1):271\u2013284. https:\/\/doi.org\/10.1104\/pp.17.01624","journal-title":"Plant Physiol"},{"issue":"4","key":"767_CR11","doi-asserted-by":"publisher","first-page":"1249","DOI":"10.1093\/jxb\/erp007","volume":"60","author":"G Cassin","year":"2009","unstructured":"Cassin G, Mari S, Curie C, Briat J-F, Czernic P (2009) Increased sensitivity to iron deficiency in Arabidopsis thaliana overaccumulating nicotianamine. J Exp Bot 60(4):1249\u20131259. https:\/\/doi.org\/10.1093\/jxb\/erp007","journal-title":"J Exp Bot"},{"issue":"7646","key":"767_CR12","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1038\/nature21417","volume":"543","author":"G Castrillo","year":"2017","unstructured":"Castrillo G, Teixeira PJPL, Paredes SH et al (2017) Root microbiota drive direct integration of phosphate stress and immunity. Nature 543(7646):513\u2013518. https:\/\/doi.org\/10.1038\/nature21417","journal-title":"Nature"},{"issue":"1","key":"767_CR13","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1007\/s10533-005-2343-3","volume":"78","author":"N Chacon","year":"2006","unstructured":"Chacon N, Silver WL, Dubinsky EA, Cusack DF (2006) Iron reduction and soil phosphorus solubilization in humid tropical forests soils: the roles of labile carbon pools and an electron shuttle compound. Biogeochemistry 78(1):67\u201384","journal-title":"Biogeochemistry"},{"issue":"1","key":"767_CR14","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/S0009-2541(02)00421-7","volume":"198","author":"S-F Cheah","year":"2003","unstructured":"Cheah S-F, Kraemer SM, Cervini-Silva J, Sposito G (2003) Steady-state dissolution kinetics of goethite in the presence of desferrioxamine B and oxalate ligands: implications for the microbial acquisition of iron. Chem Geol 198(1):63\u201375. https:\/\/doi.org\/10.1016\/S0009-2541(02)00421-7","journal-title":"Chem Geol"},{"issue":"4","key":"767_CR15","doi-asserted-by":"publisher","first-page":"1242","DOI":"10.4319\/lo.2008.53.4.1242","volume":"53","author":"TH Chrzanowski","year":"2008","unstructured":"Chrzanowski TH, Grover JP (2008) Element content of Pseudomonas fluorescens varies with growth rate and temperature: a replicated chemostat study addressing ecological stoichiometry. Limnol Oceanogr 53(4):1242\u20131251. https:\/\/doi.org\/10.4319\/lo.2008.53.4.1242","journal-title":"Limnol Oceanogr"},{"key":"767_CR16","doi-asserted-by":"publisher","unstructured":"Conaway, Amy, Igor Todorovic, Dallas L. Mould, and Deborah A. Hogan. 2024. \u201cLoss of LasR Function Leads to Decreased Repression of Pseudomonas aeruginosa PhoB Activity at Physiological Phosphate Concentrations.\u201d Preprint, March 27. https:\/\/doi.org\/10.1101\/2024.03.27.586856.","DOI":"10.1101\/2024.03.27.586856"},{"key":"767_CR17","doi-asserted-by":"publisher","unstructured":"Conway JM, Martinez PJ, Wilson ED, Del Risco NM, Dangl JL (2024) Arabidopsis Root Microbiome Microfluidic (ARMM) Device for Imaging Bacterial Colonization and Morphogenesis of Arabidopsis Roots. In Tissue Morphogenesis: Methods and Protocols, edited by Celeste M. Nelson. Springer US. https:\/\/doi.org\/10.1007\/978-1-0716-3854-5_15.","DOI":"10.1007\/978-1-0716-3854-5_15"},{"issue":"14","key":"767_CR18","doi-asserted-by":"publisher","first-page":"4492","DOI":"10.1128\/JB.186.14.4492-4501.2004","volume":"186","author":"T Danhorn","year":"2004","unstructured":"Danhorn T, Hentzer M, Givskov M, Parsek MR, Fuqua C (2004) Phosphorus limitation enhances biofilm formation of the plant pathogen Agrobacterium tumefaciens through the PhoR-PhoB regulatory system. J Bacteriol 186(14):4492\u20134501. https:\/\/doi.org\/10.1128\/JB.186.14.4492-4501.2004","journal-title":"J Bacteriol"},{"issue":"4","key":"767_CR19","doi-asserted-by":"publisher","first-page":"1368","DOI":"10.1111\/nph.19007","volume":"239","author":"GS Demirer","year":"2023","unstructured":"Demirer GS, Gibson DJ, Yue X et al (2023) Phosphate deprivation-induced changes in tomato are mediated by an interaction between brassinosteroid signaling and zinc. New Phytol 239(4):1368\u20131383. https:\/\/doi.org\/10.1111\/nph.19007","journal-title":"New Phytol"},{"issue":"1","key":"767_CR20","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1111\/pce.13872","volume":"44","author":"Na Ding","year":"2021","unstructured":"Ding Na, Huertas R, Torres-Jerez I et al (2021) Transcriptional, metabolic, physiological and developmental responses of switchgrass to phosphorus limitation. Plant Cell Environ 44(1):186\u2013202. https:\/\/doi.org\/10.1111\/pce.13872","journal-title":"Plant Cell Environ"},{"issue":"4","key":"767_CR21","doi-asserted-by":"publisher","first-page":"74","DOI":"10.3390\/soilsystems4040074","volume":"4","author":"S Doydora","year":"2020","unstructured":"Doydora S, Gatiboni L, Grieger K et al (2020) Accessing legacy phosphorus in soils. Soil Syst 4(4):74. https:\/\/doi.org\/10.3390\/soilsystems4040074","journal-title":"Soil Syst"},{"issue":"3","key":"767_CR22","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1038\/s41561-019-0530-4","volume":"13","author":"E Du","year":"2020","unstructured":"Du E, Terrer C, Pellegrini AFA et al (2020) Global patterns of terrestrial nitrogen and phosphorus limitation. Nat Geosci 13(3):221\u2013226. https:\/\/doi.org\/10.1038\/s41561-019-0530-4","journal-title":"Nat Geosci"},{"issue":"6","key":"767_CR23","doi-asserted-by":"publisher","first-page":"1755","DOI":"10.1111\/1365-2664.12915","volume":"54","author":"WJ Emsens","year":"2017","unstructured":"Emsens WJ, Aggenbach CJS, Smolders AJP, Zak D, van Diggelen R (2017) Restoration of endangered fen communities: the ambiguity of iron-phosphorus binding and phosphorus limitation. J Appl Ecol 54(6):1755\u20131764. https:\/\/doi.org\/10.1111\/1365-2664.12915","journal-title":"J Appl Ecol"},{"issue":"2","key":"767_CR24","doi-asserted-by":"publisher","first-page":"1030","DOI":"10.1104\/pp.15.01194","volume":"170","author":"S Eroglu","year":"2016","unstructured":"Eroglu S, Meier B, von Wir\u00e9n N, Peiter E (2016) The vacuolar manganese transporter MTP8 determines tolerance to iron deficiency-induced chlorosis in Arabidopsis. Plant Physiol 170(2):1030\u20131045. https:\/\/doi.org\/10.1104\/pp.15.01194","journal-title":"Plant Physiol"},{"key":"767_CR25","doi-asserted-by":"crossref","unstructured":"Evert RF, Eichhorn SE (2013a). Chapter 24 The root: sturcture and development. In Raven Biology of Plants. Peter Marshall.","DOI":"10.1007\/978-1-319-15626-8"},{"key":"767_CR26","doi-asserted-by":"crossref","unstructured":"Evert RF, Eichhorn SE (2013b) Chapter 29 plant nutrition and soils. In Raven Biology of Plants. Peter Marshall.","DOI":"10.1007\/978-1-319-15626-8"},{"issue":"March","key":"767_CR27","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1016\/j.plantsci.2018.11.011","volume":"280","author":"N Ferrol","year":"2019","unstructured":"Ferrol N, Azc\u00f3n-Aguilar C, P\u00e9rez-Tienda J (2019) Review: Arbuscular Mycorrhizas as key players in sustainable plant phosphorus acquisition: an overview on the mechanisms involved. Plant Sci Int J Exp Plant Biol 280(March):441\u2013447. https:\/\/doi.org\/10.1016\/j.plantsci.2018.11.011","journal-title":"Plant Sci Int J Exp Plant Biol"},{"issue":"April","key":"767_CR28","doi-asserted-by":"publisher","first-page":"57170","DOI":"10.3791\/57170","volume":"134","author":"J Gao","year":"2018","unstructured":"Gao J, Sasse J, Lewald KM et al (2018) Ecosystem fabrication (EcoFAB) protocols for the construction of laboratory ecosystems designed to study plant-microbe interactions. J vis Exp Jove 134(April):57170. https:\/\/doi.org\/10.3791\/57170","journal-title":"J vis Exp Jove"},{"key":"767_CR29","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.4342","author":"C Gautam","year":"2022","unstructured":"Gautam C, Tsai H, Schmidt W (2022) A quick method to quantify iron in Arabidopsis seedlings. Bio-Protoc. https:\/\/doi.org\/10.21769\/BioProtoc.4342","journal-title":"Bio-Protoc"},{"issue":"1","key":"767_CR30","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1007\/BF02080606","volume":"1","author":"FC Gerretsen","year":"1948","unstructured":"Gerretsen FC (1948) The influence of microorganisms on the phosphate intake by the plant. Plant Soil 1(1):51\u201381. https:\/\/doi.org\/10.1007\/BF02080606","journal-title":"Plant Soil"},{"issue":"1","key":"767_CR31","doi-asserted-by":"publisher","first-page":"e02194","DOI":"10.1128\/aem.02194-24","volume":"91","author":"D Giacalone","year":"2024","unstructured":"Giacalone D, Schutt E, McRose DL (2024) The phospho-ferrozine assay: a tool to study bacterial redox-active metabolites produced at the plant root. Appl Environ Microbiol 91(1):e02194-e2224. https:\/\/doi.org\/10.1128\/aem.02194-24","journal-title":"Appl Environ Microbiol"},{"issue":"3","key":"767_CR32","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1007\/s10021-001-0073-5","volume":"5","author":"R Giesler","year":"2002","unstructured":"Giesler R, Petersson T, H\u00f6gberg P (2002) Phosphorus limitation in boreal forests: effects of aluminum and iron accumulation in the humus layer. Ecosystems 5(3):300\u2013314. https:\/\/doi.org\/10.1007\/s10021-001-0073-5","journal-title":"Ecosystems"},{"issue":"2","key":"767_CR33","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1017\/S0889189300000886","volume":"1","author":"AH Goldstein","year":"1986","unstructured":"Goldstein AH (1986) Bacterial solubilization of mineral phosphates: historical perspective and future prospects. Am J Altern Agric 1(2):51\u201357. https:\/\/doi.org\/10.1017\/S0889189300000886","journal-title":"Am J Altern Agric"},{"issue":"4","key":"767_CR34","doi-asserted-by":"publisher","DOI":"10.3390\/soilsystems2040065","volume":"2","author":"A Gross","year":"2018","unstructured":"Gross A, Pett-Ridge J, Silver WL (2018) Soil oxygen limits microbial phosphorus utilization in humid tropical forest soils. Soil Syst 2(4):4. https:\/\/doi.org\/10.3390\/soilsystems2040065","journal-title":"Soil Syst"},{"issue":"12","key":"767_CR35","doi-asserted-by":"publisher","first-page":"4234","DOI":"10.1105\/tpc.111.092577","volume":"23","author":"G Grossmann","year":"2011","unstructured":"Grossmann G, Guo W-J, Ehrhardt DW et al (2011) The rootchip: an integrated microfluidic chip for plant science. Plant Cell 23(12):4234\u20134240. https:\/\/doi.org\/10.1105\/tpc.111.092577","journal-title":"Plant Cell"},{"issue":"2","key":"767_CR36","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1111\/j.1469-8137.2005.01530.x","volume":"168","author":"W Han","year":"2005","unstructured":"Han W, Fang J, Guo D, Zhang Y (2005) Leaf nitrogen and phosphorus stoichiometry across 753 terrestrial plant species in China. New Phytol 168(2):377\u2013385. https:\/\/doi.org\/10.1111\/j.1469-8137.2005.01530.x","journal-title":"New Phytol"},{"issue":"5","key":"767_CR37","doi-asserted-by":"publisher","first-page":"1207","DOI":"10.1093\/jxb\/erx454","volume":"69","author":"MT Hanlon","year":"2018","unstructured":"Hanlon MT, Ray S, Saengwilai P, Luthe D, Lynch JP, Brown KM (2018) Buffered delivery of phosphate to Arabidopsis alters responses to low phosphate. J Exp Bot 69(5):1207\u20131219. https:\/\/doi.org\/10.1093\/jxb\/erx454","journal-title":"J Exp Bot"},{"issue":"6","key":"767_CR38","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1016\/j.chom.2020.09.006","volume":"28","author":"CJ Harbort","year":"2020","unstructured":"Harbort CJ, Hashimoto M, Inoue H et al (2020) Root-secreted coumarins and the microbiota interact to improve iron nutrition in Arabidopsis. Cell Host Microbe 28(6):825-837.e6. https:\/\/doi.org\/10.1016\/j.chom.2020.09.006","journal-title":"Cell Host Microbe"},{"issue":"4","key":"767_CR39","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1007\/s13213-010-0117-1","volume":"60","author":"R Hayat","year":"2010","unstructured":"Hayat R, Ali S, Amara U, Khalid R, Ahmed I (2010) Soil beneficial bacteria and their role in plant growth promotion: a review. Ann Microbiol 60(4):579\u2013598. https:\/\/doi.org\/10.1007\/s13213-010-0117-1","journal-title":"Ann Microbiol"},{"issue":"2","key":"767_CR40","doi-asserted-by":"publisher","first-page":"921","DOI":"10.1128\/AEM.70.2.921-928.2004","volume":"70","author":"ME Hernandez","year":"2004","unstructured":"Hernandez ME, Kappler A, Newman DK (2004) Phenazines and other redox-active antibiotics promote microbial mineral reduction. Appl Environ Microbiol 70(2):921\u2013928. https:\/\/doi.org\/10.1128\/AEM.70.2.921-928.2004","journal-title":"Appl Environ Microbiol"},{"key":"767_CR41","doi-asserted-by":"publisher","unstructured":"LaRue T, Lindner H, Srinivas A, Exposito-Alonso M, Lobet G, Dinneny JR (2022) Uncovering natural variation in root system architecture and growth dynamics using a robotics-assisted phenomics platform. eLife 11 (September): e76968. https:\/\/doi.org\/10.7554\/eLife.76968.","DOI":"10.7554\/eLife.76968"},{"issue":"3","key":"767_CR42","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1139\/er-2015-0080","volume":"24","author":"M Li","year":"2016","unstructured":"Li M, Liu J, Xu Y, Qian G (2016) Phosphate adsorption on metal oxides and metal hydroxides: a comparative review. Environ Rev 24(3):319\u2013332. https:\/\/doi.org\/10.1139\/er-2015-0080","journal-title":"Environ Rev"},{"issue":"1","key":"767_CR43","doi-asserted-by":"publisher","first-page":"4413","DOI":"10.1038\/s41467-025-59637-x","volume":"16","author":"C Li","year":"2025","unstructured":"Li C, Sheng Hu, Tan M et al (2025) Rhythmic radial oxygen loss enhances soil phosphorus bioavailability. Nat Commun 16(1):4413. https:\/\/doi.org\/10.1038\/s41467-025-59637-x","journal-title":"Nat Commun"},{"issue":"1","key":"767_CR44","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1007\/s11104-021-05010-y","volume":"466","author":"JP Lynch","year":"2021","unstructured":"Lynch JP, Strock CF, Schneider HM et al (2021) Root anatomy and soil resource capture. Plant Soil 466(1):21\u201363. https:\/\/doi.org\/10.1007\/s11104-021-05010-y","journal-title":"Plant Soil"},{"issue":"5","key":"767_CR45","doi-asserted-by":"publisher","first-page":"1036","DOI":"10.3390\/microorganisms9051036","volume":"9","author":"D Lyu","year":"2021","unstructured":"Lyu D, Msimbira LA, Nazari M et al (2021) The coevolution of plants and microbes underpins sustainable agriculture. Microorganisms 9(5):1036. https:\/\/doi.org\/10.3390\/microorganisms9051036","journal-title":"Microorganisms"},{"key":"767_CR46","doi-asserted-by":"publisher","unstructured":"Malhotra, Hina, Vandana, Sandeep Sharma, and Renu Pandey. 2018. \u201cPhosphorus Nutrition: Plant Growth in Response to Deficiency and Excess.\u201d In Plant Nutrients and Abiotic Stress Tolerance, edited by Mirza Hasanuzzaman, Masayuki Fujita, Hirosuke Oku, Kamrun Nahar, and Barbara Hawrylak-Nowak. Springer. https:\/\/doi.org\/10.1007\/978-981-10-9044-8_7.","DOI":"10.1007\/978-981-10-9044-8_7"},{"key":"767_CR47","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.jcis.2013.06.049","volume":"407","author":"M Mallet","year":"2013","unstructured":"Mallet M, Barth\u00e9l\u00e9my K, Ruby C, Renard A, Naille S (2013) Investigation of phosphate adsorption onto ferrihydrite by X-ray photoelectron spectroscopy. J Colloid Interface Sci 407:95\u2013101. https:\/\/doi.org\/10.1016\/j.jcis.2013.06.049","journal-title":"J Colloid Interface Sci"},{"issue":"17","key":"767_CR48","doi-asserted-by":"publisher","first-page":"4549","DOI":"10.1073\/pnas.1618584114","volume":"114","author":"H Massalha","year":"2017","unstructured":"Massalha H, Korenblum E, Malitsky S, Shapiro OH, Aharoni A (2017) Live imaging of root-bacteria interactions in a microfluidics setup. Proc Natl Acad Sci U S A 114(17):4549\u20134554. https:\/\/doi.org\/10.1073\/pnas.1618584114","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6533","key":"767_CR49","doi-asserted-by":"publisher","first-page":"1033","DOI":"10.1126\/science.abd1515","volume":"371","author":"DL McRose","year":"2021","unstructured":"McRose DL, Newman DK (2021) Redox-active antibiotics enhance phosphorus bioavailability. Science 371(6533):1033\u20131037. https:\/\/doi.org\/10.1126\/science.abd1515","journal-title":"Science"},{"issue":"17","key":"767_CR50","doi-asserted-by":"publisher","first-page":"3262","DOI":"10.1039\/C4LC00495G","volume":"14","author":"AS Nezhad","year":"2014","unstructured":"Nezhad AS (2014) Microfluidic platforms for plant cells studies. Lab Chip 14(17):3262\u20133274. https:\/\/doi.org\/10.1039\/C4LC00495G","journal-title":"Lab Chip"},{"issue":"12","key":"767_CR51","doi-asserted-by":"publisher","first-page":"2904","DOI":"10.3390\/microorganisms11122904","volume":"11","author":"L Pan","year":"2023","unstructured":"Pan L, Cai B (2023) Phosphate-solubilizing bacteria: advances in their physiology, molecular mechanisms and microbial community effects. Microorganisms 11(12):2904. https:\/\/doi.org\/10.3390\/microorganisms11122904","journal-title":"Microorganisms"},{"issue":"1","key":"767_CR52","doi-asserted-by":"publisher","DOI":"10.3390\/soilsystems6010006","volume":"6","author":"CJ Penn","year":"2022","unstructured":"Penn CJ, Williams MR, Camberato J, Wenos N, Wason H (2022) Desorption kinetics of legacy soil phosphorus: implications for non-point transport and plant uptake. Soil Syst 6(1):1. https:\/\/doi.org\/10.3390\/soilsystems6010006","journal-title":"Soil Syst"},{"issue":"5\u20136","key":"767_CR53","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1080\/01965581.1988.10749544","volume":"6","author":"NM Price","year":"1989","unstructured":"Price NM, Harrison GI, Hering JG et al (1989) Preparation and chemistry of the artificial algal culture medium Aquil. Biol Oceanogr 6(5\u20136):443\u2013461. https:\/\/doi.org\/10.1080\/01965581.1988.10749544","journal-title":"Biol Oceanogr"},{"key":"767_CR54","doi-asserted-by":"publisher","DOI":"10.1093\/pnasnexus\/pgaf118","author":"L Quinn","year":"2025","unstructured":"Quinn L, Sharma K, Faber KT, Orphan VJ (2025) Clear as mud redefined: tunable transparent mineral scaffolds for visualizing microbial processes below ground. PNAS Nexus. https:\/\/doi.org\/10.1093\/pnasnexus\/pgaf118","journal-title":"PNAS Nexus"},{"issue":"5","key":"767_CR55","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1038\/s41589-018-0019-2","volume":"14","author":"J Rajniak","year":"2018","unstructured":"Rajniak J, Giehl RFH, Chang E, Murgia I, Von Wir\u00e9n N, Sattely ES (2018) Biosynthesis of redox-active metabolites in response to iron deficiency in plants. Nat Chem Biol 14(5):442\u2013450. https:\/\/doi.org\/10.1038\/s41589-018-0019-2","journal-title":"Nat Chem Biol"},{"issue":"30","key":"767_CR56","doi-asserted-by":"publisher","first-page":"11001","DOI":"10.1073\/pnas.0403588101","volume":"101","author":"PB Reich","year":"2004","unstructured":"Reich PB, Oleksyn J (2004) Global patterns of plant leaf N and P in relation to temperature and latitude. Proc Natl Acad Sci U S A 101(30):11001\u201311006. https:\/\/doi.org\/10.1073\/pnas.0403588101","journal-title":"Proc Natl Acad Sci U S A"},{"key":"767_CR57","doi-asserted-by":"publisher","unstructured":"Reichard, P. U., R. Kretzschmar, and S. M. Kraemer. 2007. \u201cDissolution Mechanisms of Goethite in the Presence of Siderophores and Organic Acids.\u201d Geochimica et Cosmochimica Acta, Physical Chemistry of Soils and Aquifers: A Special Issue in Honor of Garrison Sposito, vol. 71 (23): 5635\u20135650. https:\/\/doi.org\/10.1016\/j.gca.2006.12.022.","DOI":"10.1016\/j.gca.2006.12.022"},{"issue":"3","key":"767_CR58","doi-asserted-by":"publisher","first-page":"1145","DOI":"10.1104\/pp.19.01549","volume":"183","author":"RS Reis","year":"2020","unstructured":"Reis RS, Deforges J, Sokoloff T, Poirier Y (2020) Modulation of shoot phosphate level and growth by PHOSPHATE1 upstream open reading frame1. Plant Physiol 183(3):1145\u20131156. https:\/\/doi.org\/10.1104\/pp.19.01549","journal-title":"Plant Physiol"},{"key":"767_CR59","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.07597","volume":"4","author":"R Rell\u00e1n-\u00c1lvarez","year":"2015","unstructured":"Rell\u00e1n-\u00c1lvarez R, Lobet G, Lindner H et al (2015) GLO-roots: an imaging platform enabling multidimensional characterization of soil-grown root systems. Elife 4:e07597. https:\/\/doi.org\/10.7554\/eLife.07597","journal-title":"Elife"},{"issue":"1","key":"767_CR60","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1007\/s11099-008-0019-7","volume":"46","author":"RJ Ritchie","year":"2008","unstructured":"Ritchie RJ (2008) Universal chlorophyll equations for estimating chlorophylls a, b, c, and d and total chlorophylls in natural assemblages of photosynthetic organisms using acetone, methanol, or ethanol solvents. Photosynthetica 46(1):115\u2013126. https:\/\/doi.org\/10.1007\/s11099-008-0019-7","journal-title":"Photosynthetica"},{"issue":"10","key":"767_CR61","doi-asserted-by":"publisher","first-page":"3518","DOI":"10.1128\/AEM.04167-14","volume":"81","author":"S Romano","year":"2015","unstructured":"Romano S, Schulz-Vogt HN, Gonz\u00e1lez JM, Bondarev V (2015) Phosphate limitation induces drastic physiological changes, virulence-related gene expression, and secondary metabolite production in Pseudovibrio sp. strain FO-BEG1. Appl Environ Microbiol 81(10):3518\u20133528. https:\/\/doi.org\/10.1128\/AEM.04167-14","journal-title":"Appl Environ Microbiol"},{"key":"767_CR62","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00402","author":"F Santos-Beneit","year":"2015","unstructured":"Santos-Beneit F (2015) The pho regulon: a huge regulatory network in bacteria. Front Microbiol. https:\/\/doi.org\/10.3389\/fmicb.2015.00402","journal-title":"Front Microbiol"},{"key":"767_CR63","doi-asserted-by":"publisher","DOI":"10.1002\/9783527613229","volume-title":"Iron oxides in the laboratory: preparation and characterization","author":"U Schwertmann","year":"2000","unstructured":"Schwertmann U, Cornell RM (2000) Iron oxides in the laboratory: preparation and characterization, 2nd edn. Weinheim, Wiley. https:\/\/doi.org\/10.1002\/9783527613229","edition":"2"},{"key":"767_CR64","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.56275","volume":"9","author":"K Sharma","year":"2020","unstructured":"Sharma K, Palatinszky M, Nikolov G, Berry D, Shank EA (2020) Transparent soil microcosms for live-cell imaging and non-destructive stable isotope probing of soil microorganisms. Elife 9:e56275. https:\/\/doi.org\/10.7554\/eLife.56275","journal-title":"Elife"},{"key":"767_CR65","doi-asserted-by":"publisher","unstructured":"Singh, Anil, Anjana Sisodia, Vandana Sisodia, and Minakshi Padhi. 2019. \u201cChapter 4 - Role of Microbes in Restoration Ecology and Ecosystem Services.\u201d In New and Future Developments in Microbial Biotechnology and Bioengineering, edited by Jay Shankar Singh and D. P. Singh. Elsevier. https:\/\/doi.org\/10.1016\/B978-0-444-64191-5.00004-3.","DOI":"10.1016\/B978-0-444-64191-5.00004-3"},{"issue":"1","key":"767_CR66","doi-asserted-by":"publisher","DOI":"10.1111\/rec.13688","volume":"31","author":"V Singh Rawat","year":"2023","unstructured":"Singh Rawat V, Kaur J, Bhagwat S, Arora Pandit M, Dogra Rawat C (2023) Deploying microbes as drivers and indicators in ecological restoration. Restor Ecol 31(1):e13688. https:\/\/doi.org\/10.1111\/rec.13688","journal-title":"Restor Ecol"},{"issue":"5","key":"767_CR67","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10050944","volume":"10","author":"D Smercina","year":"2022","unstructured":"Smercina D, Zambare N, Hofmockel K et al (2022) Synthetic soil aggregates: bioprinted habitats for high-throughput microbial metaphenomics. Microorganisms 10(5):5. https:\/\/doi.org\/10.3390\/microorganisms10050944","journal-title":"Microorganisms"},{"issue":"4593","key":"767_CR68","doi-asserted-by":"publisher","first-page":"994","DOI":"10.1038\/180994a0","volume":"180","author":"JI Sperber","year":"1957","unstructured":"Sperber JI (1957) Solution of mineral phosphates by soil bacteria. Nature 180(4593):994\u2013995. https:\/\/doi.org\/10.1038\/180994a0","journal-title":"Nature"},{"key":"767_CR69","doi-asserted-by":"publisher","first-page":"3233","DOI":"10.1016\/0016-7037(92)90301-X","volume":"56","author":"W Stumm","year":"1992","unstructured":"Stumm W, Sulzberger B (1992) The cycling of iron in natural environments: considerations based on laboratory studies of heterogeneous redox processes. Geochim Cosmochim Acta 56:3233\u20133257. https:\/\/doi.org\/10.1016\/0016-7037(92)90301-X","journal-title":"Geochim Cosmochim Acta"},{"issue":"1","key":"767_CR70","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.marchem.2005.06.003","volume":"98","author":"D Tang","year":"2006","unstructured":"Tang D, Morel FMM (2006) Distinguishing between cellular and Fe-oxide-associated trace elements in phytoplankton. Mar Chem 98(1):18\u201330. https:\/\/doi.org\/10.1016\/j.marchem.2005.06.003","journal-title":"Mar Chem"},{"issue":"5248","key":"767_CR71","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1126\/science.271.5248.493","volume":"271","author":"P Van Cappellen","year":"1996","unstructured":"Van Cappellen P, Ingall ED (1996) Redox stabilization of the atmosphere and oceans by phosphorus-limited marine productivity. Science 271(5248):493\u2013496. https:\/\/doi.org\/10.1126\/science.271.5248.493","journal-title":"Science"},{"issue":"25","key":"767_CR72","doi-asserted-by":"publisher","first-page":"12558","DOI":"10.1073\/pnas.1820691116","volume":"116","author":"MJEEE Voges","year":"2019","unstructured":"Voges MJEEE, Bai Y, Schulze-Lefert P, Sattely ES (2019) Plant-derived coumarins shape the composition of an Arabidopsis synthetic root microbiome. Proc Natl Acad Sci U S A 116(25):12558\u201312565. https:\/\/doi.org\/10.1073\/pnas.1820691116","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6","key":"767_CR73","doi-asserted-by":"publisher","first-page":"2965","DOI":"10.1128\/AEM.68.6.2965-2971.2002","volume":"68","author":"K Vrede","year":"2002","unstructured":"Vrede K, Heldal M, Norland S, Bratbak G (2002) Elemental composition (C, N, P) and cell volume of exponentially growing and nutrient-limited bacterioplankton. Appl Environ Microbiol 68(6):2965\u20132971. https:\/\/doi.org\/10.1128\/AEM.68.6.2965-2971.2002","journal-title":"Appl Environ Microbiol"},{"issue":"18","key":"767_CR74","doi-asserted-by":"publisher","first-page":"10322","DOI":"10.1021\/es401301z","volume":"47","author":"X Wang","year":"2013","unstructured":"Wang X, Li W, Harrington R et al (2013) Effect of ferrihydrite crystallite size on phosphate adsorption reactivity. Environ Sci Technol 47(18):10322\u201310331. https:\/\/doi.org\/10.1021\/es401301z","journal-title":"Environ Sci Technol"},{"issue":"4","key":"767_CR75","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01175-18","volume":"9","author":"K Zhalnina","year":"2018","unstructured":"Zhalnina K, Zengler K, Newman D, Northen TR (2018) Need for laboratory ecosystems to unravel the structures and functions of soil microbial communities mediated by chemistry. Mbio 9(4):e01175\u201318. https:\/\/doi.org\/10.1128\/mBio.01175-18","journal-title":"Mbio"},{"issue":"2","key":"767_CR76","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1002\/sae2.12024","volume":"1","author":"L-M Zhang","year":"2022","unstructured":"Zhang L-M, Silvano E, Rihtman B et al (2022) Biochemical mechanism of phosphorus limitation impairing nitrogen fixation in diazotrophic bacterium Klebsiella variicola W12. J Sustain Agric Environ 1(2):108\u2013117. https:\/\/doi.org\/10.1002\/sae2.12024","journal-title":"J Sustain Agric Environ"}],"container-title":["BioMetals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10534-025-00767-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10534-025-00767-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10534-025-00767-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T15:43:04Z","timestamp":1769614984000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10534-025-00767-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,27]]},"references-count":76,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["767"],"URL":"https:\/\/doi.org\/10.1007\/s10534-025-00767-6","relation":{},"ISSN":["0966-0844","1572-8773"],"issn-type":[{"value":"0966-0844","type":"print"},{"value":"1572-8773","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,27]]},"assertion":[{"value":"14 June 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 October 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 November 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}