{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T16:11:01Z","timestamp":1774800661845,"version":"3.50.1"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2012,11,21]],"date-time":"2012-11-21T00:00:00Z","timestamp":1353456000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomed Microdevices"],"published-print":{"date-parts":[[2013,4]]},"DOI":"10.1007\/s10544-012-9728-6","type":"journal-article","created":{"date-parts":[[2012,11,22]],"date-time":"2012-11-22T14:15:42Z","timestamp":1353593742000},"page":"261-278","source":"Crossref","is-referenced-by-count":37,"title":["Analytical study of a microfludic DNA amplification chip using water cooling effect"],"prefix":"10.1007","volume":"15","author":[{"given":"Jyh Jian","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia Ming","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu Wei","family":"Ko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,11,21]]},"reference":[{"key":"9728_CR1","doi-asserted-by":"crossref","first-page":"2797","DOI":"10.1016\/j.applthermaleng.2011.05.005","volume":"31","author":"JJ Chen","year":"2011","unstructured":"J.J. Chen, Y.T. Yang, Modeling and experiment of shuttling speed effects on the OSTRYCH. Appl. Therm. Eng. 31, 2797\u20132807 (2011)","journal-title":"Appl. Therm. Eng."},{"key":"9728_CR2","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s10544-007-9119-6","volume":"10","author":"PC Chen","year":"2008","unstructured":"P.C. Chen, D.E. Nikitopoulos, S.A. Soper, M.C. Murphy, Temperature distribution effects on micro-CFPCR performance. Biomed. Microdevices 10, 141\u2013152 (2008)","journal-title":"Biomed. Microdevices"},{"key":"9728_CR3","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1039\/B915022F","volume":"10","author":"KH Chung","year":"2010","unstructured":"K.H. Chung, S.H. Park, Y.H. Choi, A palmtop PCR system with a disposable polymer chip operated by the thermosiphon effect. Lab Chip 10, 202\u2013210 (2010)","journal-title":"Lab Chip"},{"key":"9728_CR4","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1016\/j.bios.2008.11.009","volume":"24","author":"TH Fang","year":"2009","unstructured":"T.H. Fang, N. Ramalingam, D. Xian-Dui, T.S. Ngin, Z. Xianting, A.T. Lai Kuan, E.Y. Peng Huat, G. Hai-Qing, Real-time PCR microfluidic devices with concurrent electrochemical detection. Biosens. Bioelectron. 24, 2131\u20132136 (2009)","journal-title":"Biosens. Bioelectron."},{"key":"9728_CR5","doi-asserted-by":"crossref","first-page":"S298","DOI":"10.1016\/j.cej.2007.07.019","volume":"135S","author":"J Felbel","year":"2008","unstructured":"J. Felbel, A. Reichert, M. Kielpinski, M. Urban, T. Henkel, N. H\u00e4fner, M. D\u00fcrst, J. Weber, Reverse transcription-polymerase chain reaction (RT-PCR) in flow-through micro-reactors: thermal and fluidic concepts. Chem. Eng. J. 135S, S298\u2013S302 (2008)","journal-title":"Chem. Eng. J."},{"key":"9728_CR6","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.bios.2011.06.022","volume":"27","author":"Y Fuchiwaki","year":"2011","unstructured":"Y. Fuchiwaki, H. Nagai, M. Saito, E. Tamiya, Ultra-rapid flow-through polymerase chain reaction microfluidics using vapor pressure. Biosens. Bioelectron. 27, 88\u201394 (2011)","journal-title":"Biosens. Bioelectron."},{"key":"9728_CR7","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1039\/b406860b","volume":"4","author":"M Hashimoto","year":"2004","unstructured":"M. Hashimoto, P.C. Chen, M.W. Mitchell, D.E. Nikitopoulos, S.A. Soper, M.C. Murphy, Rapid PCR in a continuous flow device. Lab Chip 4, 638\u2013645 (2004)","journal-title":"Lab Chip"},{"key":"9728_CR8","volume-title":"Heat Transfer","author":"JP Holman","year":"2009","unstructured":"J.P. Holman, Heat Transfer, 10th edn. (McGraw-Hill, New York, 2009)","edition":"10"},{"key":"9728_CR9","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1016\/j.snb.2007.10.063","volume":"130","author":"TM Hsieh","year":"2008","unstructured":"T.M. Hsieh, C.H. Luo, F.C. Huang, J.H. Wang, L.J. Chien, G.B. Lee, Enhancement of thermal uniformity for a microthermal cycler and its application for polymerase chain reaction. Sensors Actuators B Chem. 130, 848\u2013856 (2008)","journal-title":"Sensors Actuators B Chem."},{"key":"9728_CR10","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.bej.2005.02.032","volume":"29","author":"JA Kim","year":"2006","unstructured":"J.A. Kim, J.Y. Lee, S. Seong, S.H. Cha, S.H. Lee, J.J. Kim, T.H. Park, Fabrication and characterization of a PDMS-glass hybrid continuous-flow PCR chip. Biochem. Eng. J. 29, 91\u201397 (2006)","journal-title":"Biochem. Eng. J."},{"key":"9728_CR11","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1126\/science.280.5366.1046","volume":"280","author":"MU Kopp","year":"1998","unstructured":"M.U. Kopp, A.J.D. Mello, A. Manz, Chemical amplification: continuous-flow PCR on a chip. Science 280, 1046\u20131048 (1998)","journal-title":"Science"},{"key":"9728_CR12","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s10404-010-0675-3","volume":"10","author":"Y Li","year":"2011","unstructured":"Y. Li, C. Zhang, D. Xing, Fast identification of foodborne pathogenic viruses using continuous-flow reverse transcription-PCR with fluorescence detection. Microfluid. Nanofluid. 10, 367\u2013380 (2011)","journal-title":"Microfluid. Nanofluid."},{"key":"9728_CR13","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1007\/s10404-010-0702-4","volume":"10","author":"DP Manage","year":"2011","unstructured":"D.P. Manage, Y.C. Morrissey, A.J. Stickel, J. Lauzon, A. Atrazhev, J.P. Acker, L.M. Pilarski, On-chip PCR amplification of genomic and viral templates in unprocessed whole blood. Microfluid. Nanofluid. 10, 697\u2013702 (2011)","journal-title":"Microfluid. Nanofluid."},{"key":"9728_CR14","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/s10404-007-0153-8","volume":"3","author":"S Mohr","year":"2007","unstructured":"S. Mohr, Y.H. Zhang, A. Macaskill, P.J.R. Day, R.W. Barber, N.J. Goddard, D.R. Emerson, P.R. Fielden, Numerical and experimental study of a droplet-based PCR chip. Microfluid. Nanofluid. 3, 611\u2013621 (2007)","journal-title":"Microfluid. Nanofluid."},{"key":"9728_CR15","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1007\/s00216-009-3160-7","volume":"396","author":"T Nakayama","year":"2010","unstructured":"T. Nakayama, H.M. Hiep, S. Furui, Y. Yonezawa, M. Saito, Y. Takamura, E. Tamiya, An optimal design method for preventing air bubbles in high-temperature microfluidic devices. Anal. Bioanal. Chem. 396, 457\u2013464 (2010)","journal-title":"Anal. Bioanal. Chem."},{"key":"9728_CR16","unstructured":"M.A. Northrup, M.T. Ching, R.M. White, R.T. Wltson (1993) DNA amplification in a microfabricated reaction chamber. In: Proceeding of the 7th international conference of solid state sensors and actuators, Yokohama, Japan, pp 924\u2013926"},{"key":"9728_CR17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0003-2670(03)00898-5","volume":"494","author":"PJ Obeid","year":"2003","unstructured":"P.J. Obeid, T.K. Christopoulos, Continuous-flow DNA and RNA amplification chip combined with laser-induced fluorescence detection. Anal. Chim. Acta 494, 1\u20139 (2003)","journal-title":"Anal. Chim. Acta"},{"key":"9728_CR18","doi-asserted-by":"crossref","first-page":"3170","DOI":"10.1039\/c0lc00038h","volume":"10","author":"X Qiu","year":"2010","unstructured":"X. Qiu, M.G. Mauk, D. Chen, C. Liu, H.H. Bau, A large volume, portable, real-time PCR reactor. Lab Chip 10, 3170\u20133177 (2010)","journal-title":"Lab Chip"},{"key":"9728_CR19","doi-asserted-by":"crossref","first-page":"1950","DOI":"10.1126\/science.2999980","volume":"230","author":"RK Saiki","year":"1985","unstructured":"R.K. Saiki, S. Scharf, F. Faloona, K.B. Mullis, G.T. Horn, H.A. Erlich, N. Arnheim, Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230, 1950\u20131954 (1985)","journal-title":"Science"},{"key":"9728_CR20","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1039\/B103846J","volume":"1","author":"I Schneega\u00df","year":"2001","unstructured":"I. Schneega\u00df, R. Br\u00e4utigam, J.M. K\u00f6hler, Miniaturized flow-through PCR with different template types in a silicon chip thermocycler. Lab Chip 1, 42\u201349 (2001)","journal-title":"Lab Chip"},{"key":"9728_CR21","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.sna.2005.09.024","volume":"126","author":"CY Shih","year":"2006","unstructured":"C.Y. Shih, Y. Chen, Y.C. Tai, Parylene-strengthened thermal isolation technology for microfluidic system-on-chip applications. Sensors Actuators A Phys. 126, 270\u2013276 (2006)","journal-title":"Sensors Actuators A Phys."},{"key":"9728_CR22","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/S0925-4005(02)00016-3","volume":"84","author":"K Sun","year":"2002","unstructured":"K. Sun, A. Yamaguchi, Y. Ishida, S. Matsuo, H. Misawa, A heater-integrated transparent microchannel chip for continuous-flow PCR. Sensors Actuators B Chem. 84, 283\u2013289 (2002)","journal-title":"Sensors Actuators B Chem."},{"key":"9728_CR23","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1088\/0960-1317\/15\/8\/001","volume":"15","author":"W Wang","year":"2005","unstructured":"W. Wang, Z.X. Li, R. Luo, S.H. L\u00fc, A.D. Xu, Y.J. Yang, Droplet-based micro oscillating-flow PCR chip. J. Micromech. Microeng. 15, 1369\u20131377 (2005)","journal-title":"J. Micromech. Microeng."},{"key":"9728_CR24","first-page":"76","volume":"10","author":"CT Wittwer","year":"1991","unstructured":"C.T. Wittwer, D.J. Garling, Rapid cycle DNA amplification: time and temperature optimization. Biotechniques 10, 76\u201383 (1991)","journal-title":"Biotechniques"},{"key":"9728_CR25","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1088\/0960-1317\/15\/1\/031","volume":"15","author":"M Yang","year":"2005","unstructured":"M. Yang, R. Pal, M.A. Burns, Cost-effective thermal isolation techniques for use on microfabricated DNA amplification and analysis devices. J. Micromech. Microeng. 15, 221\u2013230 (2005)","journal-title":"J. Micromech. Microeng."},{"key":"9728_CR26","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1007\/s10544-005-3999-0","volume":"7","author":"L Yao","year":"2005","unstructured":"L. Yao, B. Liu, T. Chen, S. Liu, T. Zuo, Micro flow-through PCR in a PMMA chip fabricated by KrF excimer laser. Biomed. Microdevices 7, 253\u2013257 (2005)","journal-title":"Biomed. Microdevices"},{"key":"9728_CR27","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.jcice.2007.05.001","volume":"38","author":"C Yu","year":"2007","unstructured":"C. Yu, W. Liang, I. Kuan, C. Wei, W. Gu, Fabrication and characterization of a flow-through PCR device with integrated chromium resistive heaters. J. Chin. Inst. Chem. Eng. 38, 333\u2013339 (2007)","journal-title":"J. Chin. Inst. Chem. Eng."},{"key":"9728_CR28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10544-009-9352-2","volume":"12","author":"C Zhang","year":"2010","unstructured":"C. Zhang, D. Xing, Microfluidic gradient PCR (MG-PCR): a new method for microfluidic DNA amplification. Biomed. Microdevices 12, 1\u201312 (2010)","journal-title":"Biomed. Microdevices"}],"container-title":["Biomedical Microdevices"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10544-012-9728-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10544-012-9728-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10544-012-9728-6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,31]],"date-time":"2019-05-31T07:37:33Z","timestamp":1559288253000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10544-012-9728-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,21]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["9728"],"URL":"https:\/\/doi.org\/10.1007\/s10544-012-9728-6","relation":{},"ISSN":["1387-2176","1572-8781"],"issn-type":[{"value":"1387-2176","type":"print"},{"value":"1572-8781","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,21]]}}}