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Traditional methods for <jats:italic>Legionella<\/jats:italic> detection, such as cultivation, are time-consuming, taking several days to yield valid results. Additionally, widely used bioanalytical methods like PCR lack the ability to distinguish between living and dead cells, leading to the potential for false-positive results. While dielectrophoresis has been proposed as a promising method for separating living and dead cells, our study contrasts with existing literature, revealing that the separation process and parameter characterization are non-trivial. In response to this challenge, our work introduces a novel, systematic approach of automated video analysis capable of quantifying the dielectrophoretic response of cells. By assigning a response coefficient to the dielectrophoretic effect at different conditions, our method identifies a narrow window for successful cell selection of viable <jats:italic>Legionella<\/jats:italic> cells from the non-pathogenic species <jats:italic>L. parisiensis<\/jats:italic> utilizing a microfluidic flow cell with top\u2013bottom electrodes. These findings serve as a crucial pre-step in <jats:italic>Legionella<\/jats:italic> sensing, demonstrating applicability in experiments focused on the most relevant pathogenic species, <jats:italic>L. pneumophila<\/jats:italic>. Moreover, our method can be transferred to other cell types for quantitative detection of the dielectrophoretic response and identify optimal separation parameters.<\/jats:p>","DOI":"10.1007\/s10544-025-00762-1","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:19:39Z","timestamp":1753867179000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Electronic selection of viable Legionella cells by a video-based, quantifiable dielectrophoresis approach"],"prefix":"10.1007","volume":"27","author":[{"given":"Madeline","family":"Altmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Henriksson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Neubauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Birkholz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,7,30]]},"reference":[{"key":"762_CR1","unstructured":"European Disease Prevention & Control: Legionnaires\u2019 disease. 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