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We analyzed the activities of CBE\u2010sensitive \u03b2\u2010glucosidase (GBA1) and AMP\u2010DNM\u2010sensitive \u03b2\u2010glucosidase (GBA2) in total cell lysates and PM of human fibroblast cell lines from control (normal) subjects and from patients with GD clinical types 1, 2, and 3. GBA1 activities in both total lysate and PM of GD fibroblasts were low, and their relative percentages were similar to those of control cells. In contrast, GBA2 activities were higher in GD cells than in control cells, and the degree of increase differed among the three GD types. The increase of GBA2 enzyme activity was correlated with increased expression of GBA2 protein as evaluated by QRT\u2010PCR. Activities of \u03b2\u2010galactosidase and \u03b2\u2010hexosaminidase in PM were significantly higher for GD cells than for control cells and also showed significant differences among the three GD types, suggesting the occurrence of cross\u2010talk among the enzymes involved in GSL metabolism. Our findings indicate that the profiles of glycohydrolase activities in PM may provide a valuable tool to refine the classification of GD into distinct clinical types.<\/jats:p>","DOI":"10.1007\/s10545-012-9478-x","type":"journal-article","created":{"date-parts":[[2012,4,18]],"date-time":"2012-04-18T06:42:12Z","timestamp":1334731332000},"page":"1081-1091","source":"Crossref","is-referenced-by-count":40,"title":["Cell surface associated glycohydrolases in normal and Gaucher disease fibroblasts"],"prefix":"10.1002","volume":"35","author":[{"given":"Massimo","family":"Aureli","sequence":"first","affiliation":[{"name":"Department of Medical Chemistry, Biochemistry and Biotechnology University of Milan Segrate 20090 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosaria","family":"Bassi","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Biochemistry and Biotechnology University of Milan Segrate 20090 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicoletta","family":"Loberto","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry, Biochemistry and Biotechnology University of Milan Segrate 20090 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Regis","sequence":"additional","affiliation":[{"name":"\u201cDiagnosi Pre\u2010Postnatale Malattie Metaboliche\u201d Laboratory G. 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