{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:20:30Z","timestamp":1787163630138,"version":"build-2736575974"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T00:00:00Z","timestamp":1605571200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T00:00:00Z","timestamp":1605571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"Programa Retos","award":["RTC-2016-4733-1"],"award-info":[{"award-number":["RTC-2016-4733-1"]}]},{"DOI":"10.13039\/501100005357","name":"APVV","doi-asserted-by":"crossref","award":["APVV-15-0544"],"award-info":[{"award-number":["APVV-15-0544"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Tissue Bank"],"published-print":{"date-parts":[[2021,9]]},"DOI":"10.1007\/s10561-020-09883-8","type":"journal-article","created":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T00:34:33Z","timestamp":1605573273000},"page":"379-387","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Dynamic assessment of human sperm DNA damage III: the effect of sperm freezing techniques"],"prefix":"10.1007","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2895-1249","authenticated-orcid":false,"given":"Eva","family":"Tvrd\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Gos\u00e1lvez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca","family":"Arroyo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascual","family":"S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n","family":"de Jes\u00fas Risco Delgado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal","family":"S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,17]]},"reference":[{"key":"9883_CR1","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1186\/1477-7827-12-45","volume":"12","author":"A Agarwal","year":"2014","unstructured":"Agarwal A, Tvrda E, Sharma R (2014) Relationship amongst teratozoospermia, seminal oxidative stress and male infertility. Reprod Biol Endocrinol 12:45","journal-title":"Reprod Biol Endocrinol"},{"key":"9883_CR2","doi-asserted-by":"publisher","first-page":"2007","DOI":"10.1093\/humrep\/dex281","volume":"32","author":"J Aizpurua","year":"2017","unstructured":"Aizpurua J, Medrano L, Enciso M, Sarasa J, Romero A, Fern\u00e1ndez MA, G\u00f3mez-Torres MJ (2017) New permeable cryoprotectant-free vitrification method for native human sperm. Hum Reprod 32:2007\u20132015","journal-title":"Hum Reprod"},{"key":"9883_CR3","first-page":"633","volume":"13","author":"MS Ali Mohamed","year":"2015","unstructured":"Ali Mohamed MS (2015) Slow cryopreservation is not superior to vitrification in human spermatozoa; an experimental controlled study. Iran J Reprod Med 13:633\u2013644","journal-title":"Iran J Reprod Med"},{"key":"9883_CR4","first-page":"343","volume":"21","author":"C Alvarez Sed\u00f3","year":"2017","unstructured":"Alvarez Sed\u00f3 C, Bilinski M, Lorenzi D, Uriondo H, Nobl\u00eda F, Longobucco V, Lagar EV, Nodar F (2017) Effect of sperm DNA fragmentation on embryo development: clinical and biological aspects. JBRA Assist Reprod 21:343\u2013350","journal-title":"JBRA Assist Reprod"},{"key":"9883_CR5","first-page":"227","volume":"31","author":"L Bj\u00f6rndahl","year":"2016","unstructured":"Bj\u00f6rndahl L, Barratt CLR, Mortimer D, Jouannet P (2016) \u2018How to count sperm properly\u2019: checklist for acceptability of studies based on human semen analysis. Hum Reprod 31:227\u2013232","journal-title":"Hum Reprod"},{"key":"9883_CR6","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1111\/andr.12279","volume":"5","author":"OA Bogle","year":"2017","unstructured":"Bogle OA, Kumar K, Attardo-Parrinello C, Lewis SE, Estanyol JM, Ballesc\u00e0 JL, Oliva R (2017) Identification of protein changes in human spermatozoa throughout the cryopreservation process. Andrology 5:10\u201322","journal-title":"Andrology"},{"key":"9883_CR7","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1093\/humupd\/dmp048","volume":"16","author":"TG Cooper","year":"2010","unstructured":"Cooper TG, Noonan E, von Eckardstein S, Auger J, Baker HW, Behre HM, Haugen TB, Kruger T, Wang C, Mbizvo MT, Vogelsong KM (2010) World Health Organization reference values for human semen characteristics. Hum Reprod Update 16:231\u2013245","journal-title":"Hum Reprod Update"},{"key":"9883_CR8","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1080\/15222055.2017.1281855","volume":"79","author":"R Cuevas-Uribe","year":"2017","unstructured":"Cuevas-Uribe R, Hu E, Daniels H, Gill AO, Tiersch TR (2017) Vitrification as an alternative approach for sperm cryopreservation in marine fishes. N Am J Aquac 79:187\u2013196","journal-title":"N Am J Aquac"},{"key":"9883_CR9","doi-asserted-by":"publisher","first-page":"557","DOI":"10.21037\/tau.2017.05.13","volume":"6","author":"SC Esteves","year":"2017","unstructured":"Esteves SC, Agarwal A, Majzoub A (2017) The complex nature of the sperm DNA damage process. Transl Androl Urol 6:557\u2013559","journal-title":"Transl Androl Urol"},{"key":"9883_CR10","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1111\/andr.12389","volume":"5","author":"N Gatimel","year":"2017","unstructured":"Gatimel N, Moreau J, Parinaud J, L\u00e9andri RD (2017) Sperm morphology: assessment, pathophysiology, clinical relevance, and state of the art in 2017. Andrology 5:845\u2013862","journal-title":"Andrology"},{"key":"9883_CR11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1177\/2058915815594454","volume":"4","author":"J Gos\u00e1lvez","year":"2015","unstructured":"Gos\u00e1lvez J, L\u00f3pez-Fern\u00e1ndez C, Fern\u00e1ndez JL, Esteves SC, Johnston SD (2015) Unpacking the mysteries of sperm DNA fragmentation: ten frequently asked questions. J Reprod Biotechnol Fertil 4:1\u201316","journal-title":"J Reprod Biotechnol Fertil"},{"key":"9883_CR12","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1016\/j.theriogenology.2016.02.007","volume":"86","author":"H G\u00fcrler","year":"2016","unstructured":"G\u00fcrler H, Malama E, Heppelmann M, Calisici O, Leiding C, Kastelic JP, Bollwein H (2016) Effects of cryopreservation on sperm viability, synthesis of reactive oxygen species, and DNA damage of bovine sperm. Theriogenology 86:562\u2013571","journal-title":"Theriogenology"},{"key":"9883_CR13","doi-asserted-by":"crossref","first-page":"107","DOI":"10.4103\/1008-682X.183378","volume":"19","author":"F Horta","year":"2017","unstructured":"Horta F, Alzobi H, Jitanantawittaya S, Catt S, Chen P, Pangestu M, Temple-Smith P (2017) Minimal volume vitrification of epididymal spermatozoa results in successful in vitro fertilization and embryo development in mice. Asian J Androl 19:107\u2013112","journal-title":"Asian J Androl"},{"key":"9883_CR14","doi-asserted-by":"publisher","first-page":"462","DOI":"10.2164\/jandrol.111.013789","volume":"33","author":"V Isachenko","year":"2012","unstructured":"Isachenko V, Maettner R, Petrunkina AM, Sterzik K, Mallmann P, Rahimi G, Sanchez R, Risopatron J, Damjanoski I, Isachenko E (2012) Vitrification of human ICSI\/IVF spermatozoa without cryoprotectants: new capillary technology. J Androl 33:462\u2013468","journal-title":"J Androl"},{"key":"9883_CR15","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/j.rbmo.2013.06.014","volume":"27","author":"SE Lewis","year":"2013","unstructured":"Lewis SE, John Aitken R, Conner SJ, Iuliis GD, Evenson DP, Henkel R, Giwercman A, Gharagozloo P (2013) The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment. Reprod Biomed Online 27:325\u2013337","journal-title":"Reprod Biomed Online"},{"key":"9883_CR16","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1111\/and.12246","volume":"47","author":"O Merino","year":"2015","unstructured":"Merino O, Aguag\u00fci\u00f1a WE, Esponda P, Risopatr\u00f3n J, Isachenko E, Isachenko V, S\u00e1nchez R (2015) Protective effect of butylated hydroxytoluene on sperm function in human spermatozoa cryopreserved by vitrification technique. Andrologia 47:186\u2013193","journal-title":"Andrologia"},{"key":"9883_CR17","doi-asserted-by":"publisher","first-page":"704","DOI":"10.1093\/humrep\/17.3.704","volume":"17","author":"M O'Connell","year":"2002","unstructured":"O\u2019Connell M, McClure N, Lewis SE (2002) The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Hum Reprod 17:704\u2013709","journal-title":"Hum Reprod"},{"key":"9883_CR18","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/j.mjafi.2014.09.006","volume":"70","author":"B Oberoi","year":"2014","unstructured":"Oberoi B, Kumar S, Talwar P (2014) Study of human sperm motility post cryopreservation. Med J Armed Forces India 70:349\u2013353","journal-title":"Med J Armed Forces India"},{"key":"9883_CR19","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1186\/s40104-017-0155-7","volume":"8","author":"I Ortiz","year":"2017","unstructured":"Ortiz I, Dorado J, Morrell J, Gos\u00e1lvez J, Crespo F, Jim\u00e9nez JM, Hidalgo M (2017) New approach to assess sperm DNA fragmentation dynamics: fine-tuning mathematical models. J Anim Sci Biotechnol 8:23","journal-title":"J Anim Sci Biotechnol"},{"key":"9883_CR20","first-page":"7","volume":"31","author":"NS Riva","year":"2016","unstructured":"Riva NS, Marcial L\u00f3pez CA, Mart\u00ednez G, Iaizzo RS, Artola M, Ruhlmann C (2016) An\u00e1lisis comparativo del congelamiento lento y la vitrificaci\u00f3n para la criopreservaci\u00f3n de espermatozoides. Reproducci\u00f3n 31:7\u201314","journal-title":"Reproducci\u00f3n"},{"key":"9883_CR21","doi-asserted-by":"publisher","first-page":"559","DOI":"10.2164\/jandrol.111.014274","volume":"33","author":"R Sanchez","year":"2012","unstructured":"Sanchez R, Isachenko V, Petrunkina AM, Risopatron J, Schulz M, Isachenko E (2012) Live birth after intrauterine insemination with spermatozoa from an oligoasthenozoospermic patient vitrified without permeable cryoprotectants. J Androl 33:559\u2013562","journal-title":"J Androl"},{"key":"9883_CR22","doi-asserted-by":"publisher","first-page":"162S","DOI":"10.1097\/01.AOG.0000533134.61739.52","volume":"131","author":"M Tran","year":"2018","unstructured":"Tran M, Uriondo H, Nodar F, Alvarez Sed\u00f3 C (2018) Cryopreservation promotes sperm DNA damage through oxidative stress. Obstet Gynecol 131:162S","journal-title":"Obstet Gynecol"},{"issue":"4","key":"9883_CR23","doi-asserted-by":"publisher","first-page":"e12986","DOI":"10.1111\/and.12986","volume":"50","author":"E Tvrd\u00e1","year":"2018","unstructured":"Tvrd\u00e1 E, L\u00f3pez-Fern\u00e1ndez C, S\u00e1nchez-Mart\u00edn P, Gos\u00e1lvez J (2018a) Sperm DNA fragmentation in donors and normozoospermic patients attending for a first spermiogram: Static and dynamic assessment. Andrologia 50(4):e12986","journal-title":"Andrologia"},{"key":"9883_CR24","doi-asserted-by":"publisher","first-page":"1381","DOI":"10.1007\/s11255-018-1915-9","volume":"50","author":"E Tvrd\u00e1","year":"2018","unstructured":"Tvrd\u00e1 E, Arroyo F, Gos\u00e1lvez J (2018b) Dynamic assessment of human sperm DNA damage I: the effect of seminal plasma-sperm co-incubation after ejaculation. Int Urol Nephrol 50:1381\u20131388","journal-title":"Int Urol Nephrol"},{"key":"9883_CR25","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.cryobiol.2017.10.005","volume":"79","author":"P Uribe","year":"2017","unstructured":"Uribe P, Rojas C, Meri\u00f1o J, Zambrano F, Villegas JV, Treulen F, Boguen R, Isachenko V, Isachenko E, S\u00e1nchez R (2017) Effect of incubation temperature after devitrification on quality parameters in human sperm cells. Cryobiology 79:78\u201381","journal-title":"Cryobiology"},{"key":"9883_CR26","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1007\/s10561-005-0276-7","volume":"7","author":"K W\u00fcndrich","year":"2006","unstructured":"W\u00fcndrich K, Paasch U, Leicht M, Glander HJ (2006) Activation of caspases in human spermatozoa during cryopreservation\u2014an immunoblot study. Cell Tissue Bank 7:81\u201390","journal-title":"Cell Tissue Bank"},{"key":"9883_CR27","doi-asserted-by":"publisher","first-page":"75","DOI":"10.4103\/aja.aja_19_17","volume":"20","author":"WW Zheng","year":"2018","unstructured":"Zheng WW, Song G, Wang QL, Liu SW, Zhu XL, Deng SM, Zhong A, Tan YM, Tan Y (2018) Sperm DNA damage has a negative effect on early embryonic development following in vitro fertilization. Asian J Androl 20:75\u201379","journal-title":"Asian J Androl"}],"container-title":["Cell and Tissue Banking"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10561-020-09883-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10561-020-09883-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10561-020-09883-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T09:26:51Z","timestamp":1697102811000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10561-020-09883-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,17]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["9883"],"URL":"https:\/\/doi.org\/10.1007\/s10561-020-09883-8","relation":{},"ISSN":["1389-9333","1573-6814"],"issn-type":[{"value":"1389-9333","type":"print"},{"value":"1573-6814","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,17]]},"assertion":[{"value":"9 May 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 November 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 November 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"All authors declare no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and\/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Informed consent was obtained from all individual participants included in the study.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}]}}