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The standard radiation sterilization dose of 25\u201335\u00a0kGy has been shown to induce cleavage of collagen molecules into smaller peptides and accumulation of stable crosslinks within the collagen matrix, suggesting that these mechanisms may influence radiation-induced losses in cyclic fracture resistance. The objective of this study was to determine the radiation dose-dependency of collagen chain fragmentation and crosslink accumulation within the dose range of 0\u201325\u00a0kGy. Previously, cortical bone compact tension specimens from two donor femoral pairs were divided into four treatment groups (0\u00a0kGy, 10\u00a0kGy, 17.5\u00a0kGy, and 25\u00a0kGy) and underwent cyclic loading fatigue crack propagation testing. Following fatigue testing, collagen was isolated from one compact tension specimen in each treatment group from both donors. Radiation-induced collagen chain fragmentation was assessed using SDS-PAGE (n\u2009=\u20095), and accumulation of pentosidine, pyridinoline, and non-specific advanced glycation end products were assessed using a fluorometric assay (n\u2009=\u20094). Collagen chain fragmentation increased progressively in a dose-dependent manner (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Crosslink accumulation at all radiation dose levels increased relative to the 0\u00a0kGy control but did not demonstrate dose-dependency (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Taken together with our previous findings on fatigue crack propagation behavior, these data suggest that while collagen crosslink accumulation may contribute to reduced notched fatigue behavior with irradiation, dose-dependent losses in fatigue crack propagation resistance are mainly influenced by radiation-induced chain fragmentation.<\/jats:p>","DOI":"10.1007\/s10561-024-10135-2","type":"journal-article","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T21:01:35Z","timestamp":1715806895000},"page":"735-745","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Dose-dependent effects of gamma radiation sterilization on the collagen matrix of human cortical bone allograft and its influence on fatigue crack propagation resistance"],"prefix":"10.1007","volume":"25","author":[{"given":"Dylan B.","family":"Crocker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Hering","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ozan","family":"Akkus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan E.","family":"Oest","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare M.","family":"Rimnac","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,15]]},"reference":[{"key":"10135_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-0266(01)00004-3","author":"O Akkus","year":"2001","unstructured":"Akkus O, Rimnac C (2001) Fracture resistance of gamma radiation sterilized cortical bone allografts. 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