{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T07:04:10Z","timestamp":1773903850897,"version":"3.50.1"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universit\u00e4tsklinikum Hamburg-Eppendorf (UKE)"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Tissue Bank"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The microbiological quality of corneal tissue for transplantation must be monitored in a validated procedure complying with the European Pharmacopoeia (Ph. Eur.). An automated culture system is used to detect test strains in the presence of the medium. The Ph. Eur. mentioned\n                    <jats:italic>C.\u00a0acnes<\/jats:italic>\n                    ATCC\u00a011827 strain has allegedly been isolated from skin. This strain is known to display variable growth in routine matrix validation. As the Ph. Eur. also opens the use of clinical isolates, this study analyzed the use of clinical isolates for matrix validation.\n                  <\/jats:p>\n                  <jats:p>\n                    We compared the time-to-detection (TTD) in absence and presence of cornea organ culture medium (cocm) of 24\n                    <jats:italic>C.\u00a0acnes<\/jats:italic>\n                    strains, preferring clinical isolates, in an automated culture system.\n                  <\/jats:p>\n                  <jats:p>In the first step clinical strains (n=23) were examined, thus 4\u00a0slow-growing (TTD 305,1\u2013336\u00a0h), 5\u00a0intermediate-fast-growing (TTD 250,1-305\u00a0h), 5\u00a0fast-growing strains (TTD 164,3\u2013250\u00a0h) and nine non-blood-culture-detectable strains (TTD&gt;336\u00a0h) were identified. Seven strains showed a reproducible growth signal in cocm-absence. In presence of cocm, three strains (CA02, CA10, CA22) showed a reproducible growth signal, while the reference strain was not blood-culture-detectable (bcd) in four of five experiments. The strain with the preferred growth characteristics (DSM 117854) was tested according to the Ph. Eur. conditions and showed bcd growth in all measurements (n=5) in presence of medium, in contrast to the reference strain.<\/jats:p>\n                  <jats:p>\n                    The use of a clinical isolate of\n                    <jats:italic>C. acnes<\/jats:italic>\n                    could improve matrix validation of cocm quality control testing and thus the care of cornea-transplant-patients.\n                    <jats:italic>C. acnes<\/jats:italic>\n                    DSM 117854 provides an isolate that promises more reliable results in matrix validation.\n                  <\/jats:p>","DOI":"10.1007\/s10561-026-10211-9","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T10:26:38Z","timestamp":1771496798000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimization of the microbiological quality control validation of corneal medium using a clinical C.\u00a0acnes isolate"],"prefix":"10.1007","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8144-2440","authenticated-orcid":false,"given":"Philipp Maximilian","family":"Maurer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gefion","family":"Franke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes K.","family":"Knobloch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,2,19]]},"reference":[{"key":"10211_CR1","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/j.transci.2017.05.001","volume":"56","author":"N Arlt","year":"2017","unstructured":"Arlt N, Rothe R, Juretzek T, Peltroche H, Tonn T, Moog R (2017) Detection of the relatively slow-growing Propionibacterium acnes in seven matrices of blood components and advanced therapeutical medicinal products. 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