{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T15:50:56Z","timestamp":1792857056666,"version":"4.5.0"},"reference-count":77,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T00:00:00Z","timestamp":1716163200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T00:00:00Z","timestamp":1716163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Startup Fund for Scientific Research, Fujian Medical University","award":["2021QH2028"],"award-info":[{"award-number":["2021QH2028"]}]},{"name":"Excellent Young Scholars Cultivation Project of Fujian Medical University Union Hospital","award":["2022XH026"],"award-info":[{"award-number":["2022XH026"]}]},{"name":"Joint Funds for the innovation of science and Technology, Fujian province","award":["No. 2021Y9060"],"award-info":[{"award-number":["No. 2021Y9060"]}]},{"name":"Joint Funds for the innovation of science and Technology, Fujian province","award":["2020Y9065"],"award-info":[{"award-number":["2020Y9065"]}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"crossref","award":["No. 2021J01765"],"award-info":[{"award-number":["No. 2021J01765"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Biol Toxicol"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mitochondrial dysfunction contributes to cerebral ischemia\u2013reperfusion (CI\/R) injury, which can be ameliorated by Sirtuin-3 (SIRT3). Under stress conditions, the SIRT3-promoted mitochondrial functional recovery depends on both its activity and expression. However, the approach to enhance SIRT3 activity after CI\/R injury remains unelucidated. In this study, Sprague\u2013Dawley (SD) rats were intracranially injected with either adeno-associated viral Sirtuin-1 (AAV-SIRT1) or AAV-sh_SIRT1 before undergoing transient middle cerebral artery occlusion (tMCAO). Primary cortical neurons were cultured and transfected with lentiviral SIRT1 (LV-SIRT1) and LV-sh_SIRT1 respectively before oxygen\u2013glucose deprivation\/reoxygenation (OGD\/R). Afterwards, rats and neurons were respectively treated with a selective SIRT3 inhibitor, 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP). The expression, function, and related mechanism of SIRT1 were investigated by Western Blot, flow cytometry, immunofluorescence staining, etc. After CI\/R injury, SIRT1 expression decreased in vivo and in vitro. The simulation and immune-analyses reported strong interaction between SIRT1 and SIRT3 in the cerebral mitochondria before and after CI\/R. SIRT1 overexpression enhanced SIRT3 activity by increasing the deacetylation of SIRT3, which ameliorated CI\/R-induced cerebral infarction, neuronal apoptosis, oxidative stress, neurological and motor dysfunction, and mitochondrial respiratory chain dysfunction, promoted mitochondrial biogenesis, and retained mitochondrial integrity and mitochondrial morphology. Meanwhile, SIRT1 overexpression alleviated OGD\/R-induced neuronal death and mitochondrial bioenergetic deficits. These effects were reversed by AAV-sh_SIRT1 and the neuroprotective effects of SIRT1 were partially offset by 3-TYP. These results suggest that SIRT1 restores the structure and function of mitochondria by activating SIRT3, offering neuroprotection against CI\/R injury, which signifies a potential approach for the clinical management of cerebral ischemia.<\/jats:p>\n                <jats:p><jats:bold>Graphical Abstract<\/jats:bold><\/jats:p>\n                <jats:p>1. SIRT1 is downregulated after cerebral ischemia\/reperfusion injury.<\/jats:p>\n                <jats:p>2. SIRT1 can increase the deacetylation of SIRT3 and enhance the activity of SIRT3 after cerebral ischemia\/reperfusion injury.<\/jats:p>\n                <jats:p>3. SIRT1 enhances the mitochondrial structure repair and functional recovery by activating SIRT3 after cerebral ischemia\/reperfusion injury in rats, thereby promoting neurological function.<\/jats:p>","DOI":"10.1007\/s10565-024-09869-2","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T07:01:37Z","timestamp":1716188497000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["SIRT1 restores mitochondrial structure and function in rats by activating SIRT3 after cerebral ischemia\/reperfusion injury"],"prefix":"10.1007","volume":"40","author":[{"given":"Manli","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjin","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijian","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijun","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,20]]},"reference":[{"issue":"28","key":"9869_CR1","doi-asserted-by":"publisher","first-page":"21590","DOI":"10.1074\/jbc.M109.070169","volume":"285","author":"K Aquilano","year":"2010","unstructured":"Aquilano K, Vigilanza P, Baldelli S, Pagliei B, Rotilio G, Ciriolo MR. Peroxisome proliferator-activated receptor gamma co-activator 1alpha (PGC-1alpha) and sirtuin 1 (SIRT1) reside in mitochondria: possible direct function in mitochondrial biogenesis. J Biol Chem. 2010;285(28):21590\u20139. https:\/\/doi.org\/10.1074\/jbc.M109.070169.","journal-title":"J Biol Chem"},{"issue":"5","key":"9869_CR2","doi-asserted-by":"publisher","first-page":"e331","DOI":"10.1161\/strokeaha.108.531632","volume":"40","author":"BR Broughton","year":"2009","unstructured":"Broughton BR, Reutens DC, Sobey CG. Apoptotic mechanisms after cerebral ischemia. Stroke. 2009;40(5):e331-339. https:\/\/doi.org\/10.1161\/strokeaha.108.531632.","journal-title":"Stroke"},{"key":"9869_CR3","doi-asserted-by":"publisher","unstructured":"Chen SP, Hu TH, Zhou Q, Chen TP, Yin D, He H, ... He M. Luteoloside protects the vascular endothelium against iron overload injury via the ROS\/ADMA\/DDAH II\/eNOS\/NO pathway. Chin J Nat Med. 2022;20(1):22\u201332. https:\/\/doi.org\/10.1016\/s1875-5364(21)60110-3","DOI":"10.1016\/s1875-5364(21)60110-3"},{"key":"9869_CR4","doi-asserted-by":"publisher","unstructured":"Cogliati S, Frezza C, Soriano ME, Varanita T, Quintana-Cabrera R, Corrado M, ... Scorrano L. Mitochondrial cristae shape determines respiratory chain supercomplexes assembly and respiratory efficiency. Cell. 2013;155(1):160\u2013171. https:\/\/doi.org\/10.1016\/j.cell.2013.08.032","DOI":"10.1016\/j.cell.2013.08.032"},{"issue":"2","key":"9869_CR5","first-page":"97","volume":"51","author":"IM Cojocaru","year":"2013","unstructured":"Cojocaru IM, Cojocaru M, Sapira V, Ionescu A. Evaluation of oxidative stress in patients with acute ischemic stroke. Rom J Intern Med. 2013;51(2):97\u2013106.","journal-title":"Rom J Intern Med"},{"issue":"7","key":"9869_CR6","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1111\/cns.13105","volume":"25","author":"K Cowan","year":"2019","unstructured":"Cowan K, Anichtchik O, Luo S. Mitochondrial integrity in neurodegeneration. CNS Neurosci Ther. 2019;25(7):825\u201336. https:\/\/doi.org\/10.1111\/cns.13105.","journal-title":"CNS Neurosci Ther"},{"issue":"6","key":"9869_CR7","doi-asserted-by":"publisher","first-page":"5367","DOI":"10.1021\/acsnano.7b01926","volume":"11","author":"B Esteban-Fern\u00e1ndez de \u00c1vila","year":"2017","unstructured":"Esteban-Fern\u00e1ndez de \u00c1vila B, Ram\u00edrez-Herrera DE, Campuzano S, Angsantikul P, Zhang L, Wang J. Nanomotor-enabled pH-responsive intracellular delivery of caspase-3: toward rapid cell apoptosis. ACS Nano. 2017;11(6):5367\u201374. https:\/\/doi.org\/10.1021\/acsnano.7b01926.","journal-title":"ACS Nano"},{"issue":"5","key":"9869_CR8","doi-asserted-by":"publisher","first-page":"77","DOI":"10.14715\/cmb\/2022.68.5.10","volume":"68","author":"H Gao","year":"2022","unstructured":"Gao H, Yang L, Shao Y. SIRT1\/NF-\u03baB pathway on neuronal apoptosis in rats with ischemic stroke. Cell Mol Biol (Noisy-le-grand). 2022;68(5):77\u201382. https:\/\/doi.org\/10.14715\/cmb\/2022.68.5.10.","journal-title":"Cell Mol Biol (Noisy-le-grand)"},{"key":"9869_CR9","doi-asserted-by":"publisher","first-page":"720656","DOI":"10.3389\/fcell.2021.720656","volume":"9","author":"S Geldon","year":"2021","unstructured":"Geldon S, Fern\u00e1ndez-Vizarra E, Tokatlidis K. Redox-mediated regulation of mitochondrial biogenesis, dynamics, and respiratory chain assembly in yeast and human cells. Front Cell Dev Biol. 2021;9:720656. https:\/\/doi.org\/10.3389\/fcell.2021.720656.","journal-title":"Front Cell Dev Biol"},{"issue":"10","key":"9869_CR10","doi-asserted-by":"publisher","first-page":"e3080","DOI":"10.1038\/cddis.2017.465","volume":"8","author":"L Gong","year":"2017","unstructured":"Gong L, Tang Y, An R, Lin M, Chen L, Du J. RTN1-C mediates cerebral ischemia\/reperfusion injury via ER stress and mitochondria-associated apoptosis pathways. Cell Death Dis. 2017;8(10):e3080. https:\/\/doi.org\/10.1038\/cddis.2017.465.","journal-title":"Cell Death Dis"},{"issue":"5","key":"9869_CR11","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1139\/h11-070","volume":"36","author":"BJ Gurd","year":"2011","unstructured":"Gurd BJ. Deacetylation of PGC-1alpha by SIRT1: importance for skeletal muscle function and exercise-induced mitochondrial biogenesis. Appl Physiol Nutr Metab. 2011;36(5):589\u201397. https:\/\/doi.org\/10.1139\/h11-070.","journal-title":"Appl Physiol Nutr Metab"},{"issue":"2","key":"9869_CR12","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1083\/jcb.37.2.345","volume":"37","author":"CR Hackenbrock","year":"1968","unstructured":"Hackenbrock CR. Ultrastructural bases for metabolically linked mechanical activity in mitochondria. II. Electron transport-linked ultrastructural transformations in mitochondria. J Cell Biol. 1968;37(2):345\u201369. https:\/\/doi.org\/10.1083\/jcb.37.2.345.","journal-title":"J Cell Biol"},{"issue":"1","key":"9869_CR13","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1016\/j.brainres.2006.11.075","volume":"1134","author":"K Harada","year":"2007","unstructured":"Harada K, Honmou O, Liu H, Bando M, Houkin K, Kocsis JD. Magnetic resonance lactate and lipid signals in rat brain after middle cerebral artery occlusion model. Brain Res. 2007;1134(1):206\u201313. https:\/\/doi.org\/10.1016\/j.brainres.2006.11.075.","journal-title":"Brain Res"},{"issue":"19","key":"9869_CR14","doi-asserted-by":"publisher","first-page":"3729","DOI":"10.1007\/s00018-020-03497-9","volume":"77","author":"Y He","year":"2020","unstructured":"He Y, Wu Z, Xu L, Xu K, Chen Z, Ran J, Wu L. The role of SIRT3-mediated mitochondrial homeostasis in osteoarthritis. Cell Mol Life Sci. 2020;77(19):3729\u201343. https:\/\/doi.org\/10.1007\/s00018-020-03497-9.","journal-title":"Cell Mol Life Sci"},{"key":"9869_CR15","doi-asserted-by":"publisher","unstructured":"Hern\u00e1ndez-Jim\u00e9nez M, Hurtado O, Cuartero MI, Ballesteros I, Moraga A, Pradillo JM, ... Moro MA. Silent information regulator 1 protects the brain against cerebral ischemic damage. Stroke. 2013;44(8):2333\u20132337. https:\/\/doi.org\/10.1161\/strokeaha.113.001715","DOI":"10.1161\/strokeaha.113.001715"},{"issue":"3","key":"9869_CR16","doi-asserted-by":"publisher","first-page":"174","DOI":"10.4103\/2045-9912.191365","volume":"6","author":"JL Huang","year":"2016","unstructured":"Huang JL, Manaenko A, Ye ZH, Sun XJ, Hu Q. Hypoxia therapy\u2013a new hope for the treatment of mitochondrial dysfunctions. Med Gas Res. 2016;6(3):174\u20136. https:\/\/doi.org\/10.4103\/2045-9912.191365.","journal-title":"Med Gas Res"},{"key":"9869_CR17","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.ceb.2016.02.006","volume":"38","author":"V Jayashankar","year":"2016","unstructured":"Jayashankar V, Mueller IA, Rafelski SM. Shaping the multi-scale architecture of mitochondria. Curr Opin Cell Biol. 2016;38:45\u201351. https:\/\/doi.org\/10.1016\/j.ceb.2016.02.006.","journal-title":"Curr Opin Cell Biol"},{"issue":"33","key":"9869_CR18","doi-asserted-by":"publisher","first-page":"4617","DOI":"10.1038\/onc.2010.206","volume":"29","author":"EH Jeninga","year":"2010","unstructured":"Jeninga EH, Schoonjans K, Auwerx J. Reversible acetylation of PGC-1: connecting energy sensors and effectors to guarantee metabolic flexibility. Oncogene. 2010;29(33):4617\u201324. https:\/\/doi.org\/10.1038\/onc.2010.206.","journal-title":"Oncogene"},{"issue":"1","key":"9869_CR19","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1002\/jcp.21091","volume":"213","author":"Q Jin","year":"2007","unstructured":"Jin Q, Yan T, Ge X, Sun C, Shi X, Zhai Q. Cytoplasm-localized SIRT1 enhances apoptosis. J Cell Physiol. 2007;213(1):88\u201397. https:\/\/doi.org\/10.1002\/jcp.21091.","journal-title":"J Cell Physiol"},{"key":"9869_CR20","doi-asserted-by":"publisher","unstructured":"Jumper J, Evans R, Pritzel A, Green T, Figurnov M, Ronneberger O, ... Hassabis D. Highly accurate protein structure prediction with AlphaFold. Nature. 2021;596(7873):583\u2013589. https:\/\/doi.org\/10.1038\/s41586-021-03819-2","DOI":"10.1038\/s41586-021-03819-2"},{"key":"9869_CR21","doi-asserted-by":"publisher","unstructured":"Karda R, Perocheau DP, Suff N, Ng J, Delhove JMKM, Buckley SMK, ... Waddington SN. Continual conscious bioluminescent imaging in freely moving somatotransgenic mice. Sci Rep. 2017;7(1). https:\/\/doi.org\/10.1038\/s41598-017-06696-w","DOI":"10.1038\/s41598-017-06696-w"},{"key":"9869_CR22","doi-asserted-by":"publisher","unstructured":"Koentges C, Cimolai MC, Pfeil K, Wolf D, Marchini T, Tarkhnishvili A, ... Bugger H. Impaired SIRT3 activity mediates cardiac dysfunction in endotoxemia by calpain-dependent disruption of ATP synthesis. J Mol Cell Cardiol. 2019;133:138\u2013147. https:\/\/doi.org\/10.1016\/j.yjmcc.2019.06.008","DOI":"10.1016\/j.yjmcc.2019.06.008"},{"issue":"42","key":"9869_CR23","doi-asserted-by":"publisher","first-page":"17312","DOI":"10.1074\/jbc.M117.778720","volume":"292","author":"S Kwon","year":"2017","unstructured":"Kwon S, Seok S, Yau P, Li X, Kemper B, Kemper JK. Obesity and aging diminish sirtuin 1 (SIRT1)-mediated deacetylation of SIRT3, leading to hyperacetylation and decreased activity and stability of SIRT3. J Biol Chem. 2017;292(42):17312\u201323. https:\/\/doi.org\/10.1074\/jbc.M117.778720.","journal-title":"J Biol Chem"},{"issue":"10","key":"9869_CR24","doi-asserted-by":"publisher","first-page":"2778","DOI":"10.1021\/ci200227u","volume":"51","author":"RA Laskowski","year":"2011","unstructured":"Laskowski RA, Swindells MB. LigPlot+: multiple ligand-protein interaction diagrams for drug discovery. J Chem Inf Model. 2011;51(10):2778\u201386. https:\/\/doi.org\/10.1021\/ci200227u.","journal-title":"J Chem Inf Model"},{"issue":"5","key":"9869_CR25","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.3892\/ijmm.2017.2932","volume":"39","author":"P Li","year":"2017","unstructured":"Li P, Liu Y, Burns N, Zhao KS, Song R. SIRT1 is required for mitochondrial biogenesis reprogramming in hypoxic human pulmonary arteriolar smooth muscle cells. Int J Mol Med. 2017a;39(5):1127\u201336. https:\/\/doi.org\/10.3892\/ijmm.2017.2932.","journal-title":"Int J Mol Med"},{"issue":"10","key":"9869_CR26","doi-asserted-by":"publisher","first-page":"2025","DOI":"10.1002\/jnr.24042","volume":"95","author":"PA Li","year":"2017","unstructured":"Li PA, Hou X, Hao S. Mitochondrial biogenesis in neurodegeneration. J Neurosci Res. 2017b;95(10):2025\u20139. https:\/\/doi.org\/10.1002\/jnr.24042.","journal-title":"J Neurosci Res"},{"key":"9869_CR27","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.brainresbull.2018.11.006","volume":"144","author":"Y Li","year":"2019","unstructured":"Li Y, Zhong W, Jiang Z, Tang X. New progress in the approaches for blood-brain barrier protection in acute ischemic stroke. Brain Res Bull. 2019;144:46\u201357. https:\/\/doi.org\/10.1016\/j.brainresbull.2018.11.006.","journal-title":"Brain Res Bull"},{"issue":"5\u20136","key":"9869_CR28","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1007\/s10495-020-01600-w","volume":"25","author":"WH Li","year":"2020","unstructured":"Li WH, Yang YL, Cheng X, Liu M, Zhang SS, Wang YH, Du GH. Baicalein attenuates caspase-independent cells death via inhibiting PARP-1 activation and AIF nuclear translocation in cerebral ischemia\/reperfusion rats. Apoptosis. 2020c;25(5\u20136):354\u201369. https:\/\/doi.org\/10.1007\/s10495-020-01600-w.","journal-title":"Apoptosis"},{"issue":"1","key":"9869_CR29","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1186\/s12967-020-02463-0","volume":"18","author":"Z Li","year":"2020","unstructured":"Li Z, Cui Y, Feng J, Guo Y. Identifying the pattern of immune related cells and genes in the peripheral blood of ischemic stroke. J Transl Med. 2020d;18(1):296. https:\/\/doi.org\/10.1186\/s12967-020-02463-0.","journal-title":"J Transl Med"},{"key":"9869_CR30","doi-asserted-by":"publisher","unstructured":"Li M, Li SC, Dou BK, Zou YX, Han HZ, Liu DX, ... Wang ZF. Cycloastragenol upregulates SIRT1 expression, attenuates apoptosis and suppresses neuroinflammation after brain ischemia. Acta Pharmacol Sin. 2020;41(8):1025\u20131032. https:\/\/doi.org\/10.1038\/s41401-020-0386-6","DOI":"10.1038\/s41401-020-0386-6"},{"key":"9869_CR31","doi-asserted-by":"crossref","unstructured":"Li R, Zhao J, Huang L, Yi Y, Li A, Li D, ... Liu Y. Antimicrobial peptide CGA-N12 decreases the Candida tropicalis mitochondrial membrane potential via mitochondrial permeability transition pore. Biosci Rep. 2020;40(5). 10.1042\/bsr20201007","DOI":"10.1042\/BSR20201007"},{"issue":"2","key":"9869_CR32","doi-asserted-by":"publisher","first-page":"109","DOI":"10.3233\/rnn-120228","volume":"31","author":"X Liu","year":"2013","unstructured":"Liu X, Yue R, Zhang J, Shan L, Wang R, Zhang W. Neuroprotective effects of bacopaside I in ischemic brain injury. Restor Neurol Neurosci. 2013;31(2):109\u201323. https:\/\/doi.org\/10.3233\/rnn-120228.","journal-title":"Restor Neurol Neurosci"},{"issue":"19","key":"9869_CR33","doi-asserted-by":"publisher","first-page":"2346","DOI":"10.4103\/0366-6999.215328","volume":"130","author":"AJ Liu","year":"2017","unstructured":"Liu AJ, Li B, Yang M, Liu Y, Liu YL, Su JW. Sirtuin 1 mediates hydrogen sulfide-induced cytoprotection effects in neonatal mouse cardiomyocytes. Chin Med J (engl). 2017;130(19):2346\u201353. https:\/\/doi.org\/10.4103\/0366-6999.215328.","journal-title":"Chin Med J (engl)"},{"key":"9869_CR34","doi-asserted-by":"publisher","unstructured":"Liu N, Zhang Y, Fan L, Yuan M, Du H, Cheng R, ... Lin F. Effects of transplantation with bone marrow-derived mesenchymal stem cells modified by Survivin on experimental stroke in rats. J Transl Med. 2011;9:105. https:\/\/doi.org\/10.1186\/1479-5876-9-105","DOI":"10.1186\/1479-5876-9-105"},{"key":"9869_CR35","doi-asserted-by":"publisher","unstructured":"Luo G, Jian Z, Zhu Y, Zhu Y, Chen B, Ma R, ... Xiao Y. Sirt1 promotes autophagy and inhibits apoptosis to protect cardiomyocytes from hypoxic stress. Int J Mol Med. 2019;43(5):2033\u20132043. https:\/\/doi.org\/10.3892\/ijmm.2019.4125","DOI":"10.3892\/ijmm.2019.4125"},{"issue":"5","key":"9869_CR36","doi-asserted-by":"publisher","first-page":"535","DOI":"10.17219\/acem\/133493","volume":"30","author":"C Ma","year":"2021","unstructured":"Ma C, Zhang D, Ma Q, Liu Y, Yang Y. Arbutin inhibits inflammation and apoptosis by enhancing autophagy via SIRT1. Adv Clin Exp Med. 2021;30(5):535\u201344. https:\/\/doi.org\/10.17219\/acem\/133493.","journal-title":"Adv Clin Exp Med."},{"key":"9869_CR37","doi-asserted-by":"publisher","unstructured":"Nogueira RG, Jadhav AP, Haussen DC, Bonafe A, Budzik RF, Bhuva P, ... Investigators DT. Thrombectomy 6 to 24 Hours after Stroke with a Mismatch between Deficit and Infarct. N Engl J Med. 2018;378(1):11\u201321. https:\/\/doi.org\/10.1056\/NEJMoa1706442","DOI":"10.1056\/NEJMoa1706442"},{"issue":"34","key":"9869_CR38","doi-asserted-by":"publisher","first-page":"4246","DOI":"10.2174\/1381612826666200708133912","volume":"26","author":"S Orellana-Urz\u00faa","year":"2020","unstructured":"Orellana-Urz\u00faa S, Rojas I, L\u00edbano L, Rodrigo R. Pathophysiology of ischemic stroke: role of oxidative stress. Curr Pharm Des. 2020;26(34):4246\u201360. https:\/\/doi.org\/10.2174\/1381612826666200708133912.","journal-title":"Curr Pharm Des"},{"key":"9869_CR39","doi-asserted-by":"publisher","unstructured":"Paliwal S, Chaudhuri R, Agrawal A, Mohanty S. Human tissue-specific MSCs demonstrate differential mitochondria transfer abilities that may determine their regenerative abilities. Stem Cell Res Ther. 2018;9(1). https:\/\/doi.org\/10.1186\/s13287-018-1012-0","DOI":"10.1186\/s13287-018-1012-0"},{"issue":"3","key":"9869_CR40","doi-asserted-by":"publisher","first-page":"416","DOI":"10.1111\/febs.14186","volume":"285","author":"A Pe\u00f1a-Blanco","year":"2018","unstructured":"Pe\u00f1a-Blanco A, Garc\u00eda-S\u00e1ez AJ. Bax, Bak and beyond - mitochondrial performance in apoptosis. Febs j. 2018;285(3):416\u201331. https:\/\/doi.org\/10.1111\/febs.14186.","journal-title":"Febs j"},{"issue":"9","key":"9869_CR41","doi-asserted-by":"publisher","first-page":"4892","DOI":"10.1111\/jcmm.15194","volume":"24","author":"LD Popov","year":"2020","unstructured":"Popov LD. Mitochondrial biogenesis: an update. J Cell Mol Med. 2020;24(9):4892\u20139. https:\/\/doi.org\/10.1111\/jcmm.15194.","journal-title":"J Cell Mol Med"},{"key":"9869_CR42","doi-asserted-by":"publisher","unstructured":"Prola A, Pires Da Silva J, Guilbert A, Lecru L, Piquereau J, Ribeiro M, ... Lemaire C. SIRT1 protects the heart from ER stress-induced cell death through eIF2\u03b1 deacetylation. Cell Death Differ. 2017;24(2):343\u2013356. https:\/\/doi.org\/10.1038\/cdd.2016.138","DOI":"10.1038\/cdd.2016.138"},{"issue":"1","key":"9869_CR43","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.bbrc.2017.04.088","volume":"500","author":"R Quintana-Cabrera","year":"2018","unstructured":"Quintana-Cabrera R, Mehrotra A, Rigoni G, Soriano ME. Who and how in the regulation of mitochondrial cristae shape and function. Biochem Biophys Res Commun. 2018;500(1):94\u2013101. https:\/\/doi.org\/10.1016\/j.bbrc.2017.04.088.","journal-title":"Biochem Biophys Res Commun"},{"key":"9869_CR44","doi-asserted-by":"publisher","unstructured":"Radak D, Katsiki N, Resanovic I, Jovanovic A, Sudar-Milovanovic E, Zafirovic S, ... Isenovic ER. Apoptosis and acute brain ischemia in ischemic stroke. Curr Vasc Pharmacol. 2017;15(2):115\u2013122. https:\/\/doi.org\/10.2174\/1570161115666161104095522","DOI":"10.2174\/1570161115666161104095522"},{"key":"9869_CR45","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1016\/j.neuint.2017.04.012","volume":"109","author":"I Salvatori","year":"2017","unstructured":"Salvatori I, Valle C, Ferri A, Carri MT. SIRT3 and mitochondrial metabolism in neurodegenerative diseases. Neurochem Int. 2017;109:184\u201392. https:\/\/doi.org\/10.1016\/j.neuint.2017.04.012.","journal-title":"Neurochem Int"},{"key":"9869_CR46","doi-asserted-by":"publisher","unstructured":"Shi H, Tang H, Ai W, Zeng Q, Yang H, Zhu F, ... He Q. Schisandrin B Antagonizes Cardiotoxicity Induced by Pirarubicin by Inhibiting Mitochondrial Permeability Transition Pore (mPTP) opening and decreasing cardiomyocyte apoptosis. Front Pharmacol. 2021;12:733805. https:\/\/doi.org\/10.3389\/fphar.2021.733805","DOI":"10.3389\/fphar.2021.733805"},{"key":"9869_CR47","doi-asserted-by":"publisher","unstructured":"Shiotsuki H, Yoshimi K, Shimo Y, Funayama M, Takamatsu Y, Ikeda K, ... Hattori N. A rotarod test for evaluation of motor skill learning. J Neurosci Methods. 2010;189(2):180\u2013185. https:\/\/doi.org\/10.1016\/j.jneumeth.2010.03.026","DOI":"10.1016\/j.jneumeth.2010.03.026"},{"key":"9869_CR48","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1016\/j.redox.2015.01.002","volume":"4","author":"H Sies","year":"2015","unstructured":"Sies H. Oxidative stress: a concept in redox biology and medicine. Redox Biol. 2015;4:180\u20133. https:\/\/doi.org\/10.1016\/j.redox.2015.01.002.","journal-title":"Redox Biol"},{"key":"9869_CR49","doi-asserted-by":"publisher","unstructured":"Song S, Chu L, Liang H, Chen J, Liang J, Huang Z, ... Chen X. Protective Effects of Dioscin Against Doxorubicin-Induced Hepatotoxicity Via Regulation of Sirt1\/FOXO1\/NF-\u03bab Signal. Front Pharmacol. 2019;10:1030. https:\/\/doi.org\/10.3389\/fphar.2019.01030","DOI":"10.3389\/fphar.2019.01030"},{"issue":"5","key":"9869_CR50","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1002\/1873-3468.12902","volume":"592","author":"R Stefanatos","year":"2018","unstructured":"Stefanatos R, Sanz A. The role of mitochondrial ROS in the aging brain. FEBS Lett. 2018;592(5):743\u201358. https:\/\/doi.org\/10.1002\/1873-3468.12902.","journal-title":"FEBS Lett"},{"issue":"2","key":"9869_CR51","doi-asserted-by":"publisher","first-page":"528","DOI":"10.1159\/000491810","volume":"48","author":"H Sun","year":"2018","unstructured":"Sun H, Zhong D, Wang C, Sun Y, Zhao J, Li G. MiR-298 exacerbates ischemia\/reperfusion injury following ischemic stroke by targeting Act1. Cell Physiol Biochem. 2018a;48(2):528\u201339. https:\/\/doi.org\/10.1159\/000491810.","journal-title":"Cell Physiol Biochem"},{"key":"9869_CR52","doi-asserted-by":"publisher","first-page":"7293861","DOI":"10.1155\/2018\/7293861","volume":"2018","author":"W Sun","year":"2018","unstructured":"Sun W, Liu C, Chen Q, Liu N, Yan Y, Liu B. SIRT3: a new regulator of cardiovascular diseases. Oxid Med Cell Longev. 2018b;2018:7293861. https:\/\/doi.org\/10.1155\/2018\/7293861.","journal-title":"Oxid Med Cell Longev"},{"key":"9869_CR53","doi-asserted-by":"publisher","unstructured":"Ten VS, Stepanova AA, Ratner V, Neginskaya M, Niatsetskaya Z, Sosunov S, Starkov A. Mitochondrial dysfunction and permeability transition in neonatal brain and lung injuries. Cells. 2021;10(3). https:\/\/doi.org\/10.3390\/cells10030569","DOI":"10.3390\/cells10030569"},{"key":"9869_CR54","doi-asserted-by":"publisher","unstructured":"Thomalla G, Simonsen CZ, Boutitie F, Andersen G, Berthezene Y, Cheng B, ... Investigators W-U. MRI-guided thrombolysis for stroke with unknown time of onset. N Engl J Med. 2018;379(7):611\u2013622. https:\/\/doi.org\/10.1056\/NEJMoa1804355","DOI":"10.1056\/NEJMoa1804355"},{"key":"9869_CR55","doi-asserted-by":"publisher","unstructured":"Tu LF, Cao LF, Zhang YH, Guo YL, Zhou YF, Lu WQ, ... He RR. Sirt3-dependent deacetylation of COX-1 counteracts oxidative stress-induced cell apoptosis. FASEB J. 2019;33(12):14118\u201314128. https:\/\/doi.org\/10.1096\/fj.201900708R","DOI":"10.1096\/fj.201900708R"},{"issue":"10","key":"9869_CR56","doi-asserted-by":"publisher","first-page":"6281","DOI":"10.1021\/acs.jctc.1c00645","volume":"17","author":"MS Valdes-Tresanco","year":"2021","unstructured":"Valdes-Tresanco MS, Valdes-Tresanco ME, Valiente PA, Moreno E. gmx_MMPBSA: a new tool to perform end-state free energy calculations with GROMACS. J Chem Theory Comput. 2021;17(10):6281\u201391. https:\/\/doi.org\/10.1021\/acs.jctc.1c00645.","journal-title":"J Chem Theory Comput"},{"issue":"16","key":"9869_CR57","doi-asserted-by":"publisher","first-page":"1701","DOI":"10.1002\/jcc.20291","volume":"26","author":"D Van Der Spoel","year":"2005","unstructured":"Van Der Spoel D, Lindahl E, Hess B, Groenhof G, Mark AE, Berendsen HJ. GROMACS: fast, flexible, and free. J Comput Chem. 2005;26(16):1701\u201318. https:\/\/doi.org\/10.1002\/jcc.20291.","journal-title":"J Comput Chem"},{"key":"9869_CR58","doi-asserted-by":"publisher","unstructured":"Varanita T, Soriano ME, Romanello V, Zaglia T, Quintana-Cabrera R, Semenzato M, ... Scorrano L. The OPA1-dependent mitochondrial cristae remodeling pathway controls atrophic, apoptotic, and ischemic tissue damage. Cell Metab. 2015;21(6):834\u2013844. https:\/\/doi.org\/10.1016\/j.cmet.2015.05.007","DOI":"10.1016\/j.cmet.2015.05.007"},{"issue":"2","key":"9869_CR59","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1038\/s41580-021-00415-0","volume":"23","author":"I Vercellino","year":"2022","unstructured":"Vercellino I, Sazanov LA. The assembly, regulation and function of the mitochondrial respiratory chain. Nat Rev Mol Cell Biol. 2022;23(2):141\u201361. https:\/\/doi.org\/10.1038\/s41580-021-00415-0.","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"6","key":"9869_CR60","doi-asserted-by":"publisher","first-page":"605","DOI":"10.5582\/bst.2018.01263","volume":"12","author":"C Wang","year":"2019","unstructured":"Wang C, Yang Y, Zhang Y, Liu J, Yao Z, Zhang C. Protective effects of metformin against osteoarthritis through upregulation of SIRT3-mediated PINK1\/Parkin-dependent mitophagy in primary chondrocytes. Biosci Trends. 2019;12(6):605\u201312. https:\/\/doi.org\/10.5582\/bst.2018.01263.","journal-title":"Biosci Trends"},{"key":"9869_CR61","doi-asserted-by":"publisher","unstructured":"Wang Z, Sun R, Wang G, Chen Z, Li Y, Zhao Y, ... Tian X. SIRT3-mediated deacetylation of PRDX3 alleviates mitochondrial oxidative damage and apoptosis induced by intestinal ischemia\/reperfusion injury. Redox Biol. 2020;28:101343. https:\/\/doi.org\/10.1016\/j.redox.2019.101343","DOI":"10.1016\/j.redox.2019.101343"},{"key":"9869_CR62","doi-asserted-by":"publisher","first-page":"2047","DOI":"10.2147\/DDDT.S248628","volume":"14","author":"J Wu","year":"2020","unstructured":"Wu J, Yang Y, Gao Y, Wang Z, Ma J. Melatonin Attenuates anoxia\/reoxygenation injury by inhibiting excessive mitophagy through the MT2\/SIRT3\/FoxO3a signaling pathway in H9c2 cells. Drug Des Devel Ther. 2020;14:2047\u201360. https:\/\/doi.org\/10.2147\/DDDT.S248628.","journal-title":"Drug Des Devel Ther"},{"key":"9869_CR63","doi-asserted-by":"publisher","unstructured":"Wu J, Zeng Z, Zhang W, Deng Z, Wan Y, Zhang Y, ... Chen Z. Emerging role of SIRT3 in mitochondrial dysfunction and cardiovascular diseases. Free Radic Res. 2019;53(2):139\u2013149. https:\/\/doi.org\/10.1080\/10715762.2018.1549732","DOI":"10.1080\/10715762.2018.1549732"},{"key":"9869_CR64","doi-asserted-by":"publisher","unstructured":"Xiao X, Yong L, Liu D, Yang H, Liang C, Jia X, ... Song Y. Effects of in utero exposure to lanthanum on neurological behavior in rat offspring. Neurotoxicol Teratol. 2020;77:106854. https:\/\/doi.org\/10.1016\/j.ntt.2019.106854","DOI":"10.1016\/j.ntt.2019.106854"},{"issue":"1","key":"9869_CR65","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1186\/s12967-022-03830-9","volume":"20","author":"W Xie","year":"2022","unstructured":"Xie W, Zhu T, Zhang S, Sun X. Protective effects of Gypenoside XVII against cerebral ischemia\/reperfusion injury via SIRT1-FOXO3A- and Hif1a-BNIP3-mediated mitochondrial autophagy. J Transl Med. 2022;20(1):622. https:\/\/doi.org\/10.1186\/s12967-022-03830-9.","journal-title":"J Transl Med"},{"key":"9869_CR66","doi-asserted-by":"publisher","unstructured":"Xie Z, Cao BQ, Wang T, Lei Q, Kang T, Ge CY, ... Hui H. LanCL1 attenuates ischemia-induced oxidative stress by Sirt3-mediated preservation of mitochondrial function. Brain Res Bull. 2018;142:216\u2013223. https:\/\/doi.org\/10.1016\/j.brainresbull.2018.07.017","DOI":"10.1016\/j.brainresbull.2018.07.017"},{"key":"9869_CR67","doi-asserted-by":"publisher","unstructured":"Xu D, Xia N, Hou K, Li F, Chen S, Hu Y, ... Li Y. Clematichinenoside facilitates recovery of neurological and motor function in rats after cerebral ischemic injury through inhibiting notch\/NF-\u03baB pathway. J Stroke Cerebrovasc Dis. 2019;28(11):104288. https:\/\/doi.org\/10.1016\/j.jstrokecerebrovasdis.2019.07.004","DOI":"10.1016\/j.jstrokecerebrovasdis.2019.07.004"},{"issue":"8","key":"9869_CR68","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1007\/s12264-020-00489-4","volume":"36","author":"W Yan","year":"2020","unstructured":"Yan W, Sun W, Fan J, Wang H, Han S, Li J, Yin Y. Sirt1-ROS-TRAF6 signaling-induced pyroptosis contributes to early injury in ischemic mice. Neurosci Bull. 2020;36(8):845\u201359. https:\/\/doi.org\/10.1007\/s12264-020-00489-4.","journal-title":"Neurosci Bull"},{"key":"9869_CR69","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.ejphar.2015.12.003","volume":"771","author":"Z Yang","year":"2016","unstructured":"Yang Z, Weian C, Susu H, Hanmin W. Protective effects of mangiferin on cerebral ischemia-reperfusion injury and its mechanisms. Eur J Pharmacol. 2016;771:145\u201351. https:\/\/doi.org\/10.1016\/j.ejphar.2015.12.003.","journal-title":"Eur J Pharmacol"},{"key":"9869_CR70","doi-asserted-by":"publisher","first-page":"2823454","DOI":"10.1155\/2017\/2823454","volume":"2017","author":"X Yang","year":"2017","unstructured":"Yang X, Si P, Qin H, Yin L, Yan LJ, Zhang C. The neuroprotective effects of SIRT1 on NMDA-Induced excitotoxicity. Oxid Med Cell Longev. 2017;2017:2823454. https:\/\/doi.org\/10.1155\/2017\/2823454.","journal-title":"Oxid Med Cell Longev"},{"key":"9869_CR71","doi-asserted-by":"publisher","unstructured":"Yi X, Guo W, Shi Q, Yang Y, Zhang W, Chen X, ... Li C. SIRT3-Dependent mitochondrial dynamics remodeling contributes to oxidative stress-induced melanocyte degeneration in vitiligo. Theranostics. 2019;9(6):1614\u20131633. https:\/\/doi.org\/10.7150\/thno.30398","DOI":"10.7150\/thno.30398"},{"key":"9869_CR72","doi-asserted-by":"publisher","unstructured":"Zhai M, Li B, Duan W, Jing L, Zhang B, Zhang M, ... Yu S. Melatonin ameliorates myocardial ischemia reperfusion injury through SIRT3-dependent regulation of oxidative stress and apoptosis. J Pineal Res. 2017;63(2). https:\/\/doi.org\/10.1111\/jpi.12419","DOI":"10.1111\/jpi.12419"},{"key":"9869_CR73","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.neuroscience.2011.12.053","volume":"205","author":"Q Zhang","year":"2012","unstructured":"Zhang Q, Wu Y, Zhang P, Sha H, Jia J, Hu Y, Zhu J. Exercise induces mitochondrial biogenesis after brain ischemia in rats. Neuroscience. 2012;205:10\u20137. https:\/\/doi.org\/10.1016\/j.neuroscience.2011.12.053.","journal-title":"Neuroscience"},{"key":"9869_CR74","doi-asserted-by":"publisher","first-page":"7850154","DOI":"10.1155\/2019\/7850154","volume":"2019","author":"QY Zhang","year":"2019","unstructured":"Zhang QY, Wang ZJ, Miao L, Wang Y, Chang LL, Guo W, Zhu YZ. Neuroprotective effect of SCM-198 through stabilizing endothelial cell function. Oxid Med Cell Longev. 2019;2019:7850154. https:\/\/doi.org\/10.1155\/2019\/7850154.","journal-title":"Oxid Med Cell Longev"},{"key":"9869_CR75","doi-asserted-by":"publisher","unstructured":"Zhang J, Zou Y, Cheng-Jing Y, Xiang-Heng L, Wang XP, Yu XJ, ... Wang J. Pioglitazone alleviates cisplatin nephrotoxicity by suppressing mitochondria-mediated apoptosis via SIRT1\/p53 signalling. J Cell Mol Med. 2020;24(20), 11718\u201311728. https:\/\/doi.org\/10.1111\/jcmm.15782","DOI":"10.1111\/jcmm.15782"},{"key":"9869_CR76","doi-asserted-by":"publisher","unstructured":"Zhao Q, Tian Z, Zhou G, Niu Q, Chen J, Li P, ... Wang A. SIRT1-dependent mitochondrial biogenesis supports therapeutic effects of resveratrol against neurodevelopment damage by fluoride. Theranostics. 2020;10(11):4822\u20134838. https:\/\/doi.org\/10.7150\/thno.42387","DOI":"10.7150\/thno.42387"},{"key":"9869_CR77","doi-asserted-by":"publisher","unstructured":"Zheng M, Chen R, Chen H, Zhang Y, Chen J, Lin P, ... Liu N. Netrin-1 promotes synaptic formation and axonal regeneration via JNK1\/c-Jun pathway after the middle cerebral artery occlusion. Front Cell Neurosci. 2018;12:13. https:\/\/doi.org\/10.3389\/fncel.2018.00013","DOI":"10.3389\/fncel.2018.00013"}],"container-title":["Cell Biology and Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10565-024-09869-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10565-024-09869-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10565-024-09869-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T01:28:26Z","timestamp":1735867706000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10565-024-09869-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,20]]},"references-count":77,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["9869"],"URL":"https:\/\/doi.org\/10.1007\/s10565-024-09869-2","relation":{},"ISSN":["1573-6822"],"issn-type":[{"value":"1573-6822","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,20]]},"assertion":[{"value":"9 December 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 April 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 May 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The protocols observed the guidelines of National Institute of Health (NIH Publications NO. 80\u201323, revised in 1996). The experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of Fujian Medical University Fujian, China [IACUC FJMU 2022\u20130847].","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"31"}}