{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T04:04:58Z","timestamp":1780027498393,"version":"3.53.1"},"reference-count":48,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T00:00:00Z","timestamp":1776470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"vor","delay-in-days":41,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100012669","name":"Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012669","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Biol Toxicol"],"DOI":"10.1007\/s10565-026-10193-0","type":"journal-article","created":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T16:35:19Z","timestamp":1776530119000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single-cell transcriptome profiling reveals the heterogeneity of ossification of the posterior longitudinal ligament (OPLL) and its immune microenvironment"],"prefix":"10.1007","volume":"42","author":[{"given":"Zhongyuan","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxiang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengya","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Tran Canh","family":"Tung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiharu","family":"Kawaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoyu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ManMan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xizhe","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyu","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,18]]},"reference":[{"issue":"1","key":"10193_CR1","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.spinee.2024.08.030","volume":"25","author":"H Alaa","year":"2025","unstructured":"Alaa H, Tung NTC, Ueno T, et al. Importance of gap evaluation in the ossification of posterior longitudinal ligament lesions using 3-dimensional computed tomography. Spine J. 2025;25(1):69\u201379.","journal-title":"Spine J"},{"key":"10193_CR2","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.00896","volume":"11","author":"Z Cai","year":"2020","unstructured":"Cai Z, Liu W, Chen K, et al. Aberrantly expressed lncRNAs and mRNAs of osteogenically differentiated mesenchymal stem cells in ossification of the posterior longitudinal ligament. Front Genet. 2020;11:896.","journal-title":"Front Genet"},{"issue":"8","key":"10193_CR3","doi-asserted-by":"publisher","first-page":"1757","DOI":"10.1007\/s00586-018-5663-4","volume":"27","author":"S Chen","year":"2018","unstructured":"Chen S, Zhu H, Wang G, Xie Z, Wang J, Chen J. Combined use of leptin and mechanical stress has osteogenic effects on ossification of the posterior longitudinal ligament. Eur Spine J. 2018;27(8):1757\u201366.","journal-title":"Eur Spine J"},{"issue":"7","key":"10193_CR4","doi-asserted-by":"publisher","first-page":"3993","DOI":"10.3390\/ijms23073993","volume":"23","author":"H Cherif","year":"2022","unstructured":"Cherif H, Mannarino M, Pacis AS, et al. Single-Cell RNA-Seq Analysis of Cells from Degenerating and Non-Degenerating Intervertebral Discs from the Same Individual Reveals New Biomarkers for Intervertebral Disc Degeneration. Int J Mol Sci. 2022;23(7):3993.","journal-title":"Int J Mol Sci"},{"issue":"7","key":"10193_CR5","doi-asserted-by":"publisher","first-page":"2021","DOI":"10.3390\/biomedicines11072021","volume":"11","author":"LY Fay","year":"2023","unstructured":"Fay LY, Kuo CH, Chang HK, et al. Comparative study of the cytokine profiles of serum and tissues from patients with the ossification of the posterior longitudinal ligament. Biomedicines. 2023;11(7):2021.","journal-title":"Biomedicines"},{"issue":"12","key":"10193_CR6","doi-asserted-by":"publisher","first-page":"6354","DOI":"10.1172\/JCI132518","volume":"130","author":"H Feng","year":"2020","unstructured":"Feng H, Xing W, Han Y, et al. Tendon-derived cathepsin K-expressing progenitor cells activate Hedgehog signaling to drive heterotopic ossification. J Clin Invest. 2020;130(12):6354\u201365.","journal-title":"J Clin Invest"},{"issue":"10","key":"10193_CR7","doi-asserted-by":"publisher","first-page":"1461","DOI":"10.1016\/j.spinee.2023.06.400","volume":"23","author":"R Fujita","year":"2023","unstructured":"Fujita R, Endo T, Takahata M, et al. High whole-body bone mineral density in ossification of the posterior longitudinal ligament. Spine J: Off J North Am Spine Soc. 2023;23(10):1461\u201370.","journal-title":"Spine J: Off J North Am Spine Soc"},{"issue":"1","key":"10193_CR8","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-021-00163-z","volume":"9","author":"Y Gan","year":"2021","unstructured":"Gan Y, He J, Zhu J, et al. Spatially defined single-cell transcriptional profiling characterizes diverse chondrocyte subtypes and nucleus pulposus progenitors in human intervertebral discs. Bone Res. 2021;9(1):37.","journal-title":"Bone Res"},{"issue":"12","key":"10193_CR9","doi-asserted-by":"publisher","first-page":"1490","DOI":"10.1038\/s41556-019-0417-z","volume":"21","author":"T Harvey","year":"2019","unstructured":"Harvey T, Flamenco S, Fan CM. A Tppp3(+)Pdgfra(+) tendon stem cell population contributes to regeneration and reveals a shared role for PDGF signalling in regeneration and fibrosis. Nat Cell Biol. 2019;21(12):1490\u2013503.","journal-title":"Nat Cell Biol"},{"issue":"2","key":"10193_CR10","doi-asserted-by":"publisher","first-page":"651","DOI":"10.14245\/ns.2346124.062","volume":"20","author":"Z He","year":"2023","unstructured":"He Z, Tung NTC, Makino H, et al. Assessment of cervical myelopathy risk in ossification of the posterior longitudinal ligament patients with spinal cord compression based on segmental dynamic versus static factors. Neurospine. 2023a;20(2):651\u201361.","journal-title":"Neurospine"},{"issue":"8","key":"10193_CR11","doi-asserted-by":"publisher","first-page":"2268","DOI":"10.1093\/rheumatology\/kead555","volume":"63","author":"Z He","year":"2023","unstructured":"He Z, Tung NTC, Yahara Y, et al. Association between serum interleukin-17 levels and ectopic bone formation in OPLL patients with DISH. Rheumatology (Oxford, England). 2023;63(8):2268\u201377.","journal-title":"Rheumatology (Oxford, England)"},{"issue":"1","key":"10193_CR12","doi-asserted-by":"publisher","first-page":"e2310685120","DOI":"10.1073\/pnas.2310685120","volume":"121","author":"H Hu","year":"2024","unstructured":"Hu H, Luo S, Lai P, et al. ANGPTL4 binds to the leptin receptor to regulate ectopic bone formation. Proc Natl Acad Sci USA. 2024;121(1):e2310685120.","journal-title":"Proc Natl Acad Sci USA"},{"issue":"1","key":"10193_CR13","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1038\/s41536-021-00178-4","volume":"6","author":"Y Huang","year":"2021","unstructured":"Huang Y, Wang X, Zhou D, Zhou W, Dai F, Lin H. Macrophages in heterotopic ossification: from mechanisms to therapy. NPJ Regen Med. 2021;6(1):70.","journal-title":"NPJ Regen Med"},{"issue":"2","key":"10193_CR14","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2024.101241","volume":"12","author":"C Huang","year":"2025","unstructured":"Huang C, Zeng B, Zhou B, et al. Single-cell transcriptomic analysis of chondrocytes in cartilage and pathogenesis of osteoarthritis. Genes Dis. 2025;12(2):101241.","journal-title":"Genes Dis"},{"issue":"17","key":"10193_CR15","doi-asserted-by":"publisher","first-page":"1600","DOI":"10.2106\/JBJS.23.01475","volume":"106","author":"S Ito","year":"2024","unstructured":"Ito S, Nakashima H, Segi N, et al. Ten-year follow-up of posterior decompression and fusion surgery for thoracic ossification of the posterior longitudinal ligament. J Bone Joint Surg. 2024;106(17):1600\u20139.","journal-title":"J Bone Joint Surg"},{"issue":"5","key":"10193_CR16","doi-asserted-by":"publisher","first-page":"552","DOI":"10.1007\/s11914-023-00814-6","volume":"21","author":"H Kato","year":"2023","unstructured":"Kato H, Braddock DT, Ito N. Genetics of diffuse idiopathic skeletal hyperostosis and ossification of the spinal ligaments. Curr Osteoporos Rep. 2023;21(5):552\u201366.","journal-title":"Curr Osteoporos Rep"},{"issue":"5","key":"10193_CR17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0174881","volume":"12","author":"Y Kawaguchi","year":"2017","unstructured":"Kawaguchi Y, Nakano M, Yasuda T, et al. Serum biomarkers in patients with ossification of the posterior longitudinal ligament (OPLL): inflammation in OPLL. PLoS ONE. 2017;12(5):e0174881.","journal-title":"PLoS ONE"},{"key":"10193_CR18","doi-asserted-by":"publisher","first-page":"e86514","DOI":"10.7554\/eLife.86514","volume":"12","author":"Y Koike","year":"2023","unstructured":"Koike Y, Takahata M, Nakajima M, et al. Genetic insights into ossification of the posterior longitudinal ligament of the spine. eLife. 2023;12:e86514.","journal-title":"eLife"},{"issue":"10","key":"10193_CR19","doi-asserted-by":"publisher","first-page":"3480","DOI":"10.7150\/thno.85077","volume":"13","author":"J Li","year":"2023","unstructured":"Li J, Fan C, Lv Z, et al. Microtubule stabilization targeting regenerative chondrocyte cluster for cartilage regeneration. Theranostics. 2023;13(10):3480\u201396.","journal-title":"Theranostics"},{"issue":"1","key":"10193_CR20","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-021-00175-9","volume":"10","author":"J Lin","year":"2022","unstructured":"Lin J, Yang Y, Zhou W, et al. Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx(-\/-) mice. Bone Res. 2022;10(1):4.","journal-title":"Bone Res"},{"issue":"10","key":"10193_CR21","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.13464","volume":"56","author":"P Lin","year":"2023","unstructured":"Lin P, Yan P, Zhu J, et al. Spatially multicellular variability of intervertebral disc degeneration by comparative single-cell analysis. Cell Prolif. 2023;56(10):e13464.","journal-title":"Cell Prolif"},{"key":"10193_CR22","doi-asserted-by":"publisher","first-page":"833420","DOI":"10.3389\/fcell.2021.833420","volume":"9","author":"Z Ling","year":"2021","unstructured":"Ling Z, Liu Y, Wang Z, et al. Single-Cell RNA-Seq analysis reveals macrophage involved in the progression of human intervertebral disc degeneration. Front Cell Dev Biol. 2021;9:833420.","journal-title":"Front Cell Dev Biol"},{"key":"10193_CR23","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/j.ejphar.2017.07.013","volume":"810","author":"X Liu","year":"2017","unstructured":"Liu X, Kumagai G, Wada K, et al. Suppression of osteogenic differentiation in mesenchymal stem cells from patients with ossification of the posterior longitudinal ligament by a histamine-2-receptor antagonist. Eur J Pharmacol. 2017;810:156\u201362.","journal-title":"Eur J Pharmacol"},{"issue":"1","key":"10193_CR24","doi-asserted-by":"publisher","DOI":"10.1007\/s10143-024-02735-z","volume":"47","author":"WKT Liu","year":"2024","unstructured":"Liu WKT, Yuet Siu KH, Cheung JP, Shea GK. Radiographic characterization of OPLL progression in patients receiving laminoplasty with a minimum of two-years follow-up. Neurosurg Rev. 2024a;47(1):505.","journal-title":"Neurosurg Rev"},{"issue":"6","key":"10193_CR25","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.1007\/s11684-024-1075-5","volume":"18","author":"X Liu","year":"2024","unstructured":"Liu X, Zhang L, Wang G, et al. Single-cell transcriptome profiling identifies the activation of type I interferon signaling in ossified posterior longitudinal ligament. Front Med. 2024;18(6):1087\u201399.","journal-title":"Front Med"},{"issue":"1","key":"10193_CR26","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1002\/jbmr.203","volume":"26","author":"X Lu","year":"2011","unstructured":"Lu X, Beck GR Jr, Gilbert LC, et al. Identification of the homeobox protein Prx1 (MHox, Prrx-1) as a regulator of osterix expression and mediator of tumor necrosis factor \u03b1 action in osteoblast differentiation. J Bone Min Res: Off J Am Soc Bone Min Res. 2011;26(1):209\u201319.","journal-title":"J Bone Min Res: Off J Am Soc Bone Min Res"},{"issue":"3","key":"10193_CR27","doi-asserted-by":"publisher","first-page":"544","DOI":"10.14245\/ns.19edi.014","volume":"16","author":"A Moghaddamjou","year":"2019","unstructured":"Moghaddamjou A, Fehlings MG. An age-old debate: anterior versus posterior surgery for ossification of the posterior longitudinal ligament. Neurospine. 2019;16(3):544\u20137.","journal-title":"Neurospine"},{"issue":"1","key":"10193_CR28","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/j.ajhg.2016.05.018","volume":"99","author":"M Nakajima","year":"2016","unstructured":"Nakajima M, Kou I, Ohashi H, Ikegawa S. Identification and functional characterization of RSPO2 as a susceptibility gene for ossification of the posterior longitudinal ligament of the spine. Am J Hum Genet. 2016;99(1):202\u20137.","journal-title":"Am J Hum Genet"},{"issue":"4","key":"10193_CR29","doi-asserted-by":"publisher","first-page":"638","DOI":"10.1016\/j.ajhg.2023.03.004","volume":"110","author":"M Nakajima","year":"2023","unstructured":"Nakajima M, Koido M, Guo L, Terao C, Ikegawa S. A novel CCDC91 isoform associated with ossification of the posterior longitudinal ligament of the spine works as a non-coding RNA to regulate osteogenic genes. Am J Hum Genet. 2023;110(4):638\u201347.","journal-title":"Am J Hum Genet"},{"issue":"1","key":"10193_CR30","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-60248-3","volume":"10","author":"Y Oshima","year":"2020","unstructured":"Oshima Y, Doi T, Kato S, et al. Association between ossification of the longitudinal ligament of the cervical spine and arteriosclerosis in the carotid artery. Sci Rep. 2020;10(1):3369.","journal-title":"Sci Rep"},{"issue":"4","key":"10193_CR31","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1097\/BRS.0000000000003240","volume":"45","author":"S Park","year":"2020","unstructured":"Park S, Lee DH, Ahn J, et al. How does ossification of posterior longitudinal ligament progress in conservatively managed patients? Spine. 2020;45(4):234\u201343.","journal-title":"Spine"},{"key":"10193_CR32","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiomed.2023.106926","volume":"160","author":"Y Qu","year":"2023","unstructured":"Qu Y, Wang Y, Wang S, et al. A comprehensive analysis of single-cell RNA transcriptome reveals unique SPP1+ chondrocytes in human osteoarthritis. Comput Biol Med. 2023;160:106926.","journal-title":"Comput Biol Med"},{"issue":"6","key":"10193_CR33","doi-asserted-by":"publisher","first-page":"E78","DOI":"10.1097\/BRS.0000000000004557","volume":"48","author":"H Saito","year":"2023","unstructured":"Saito H, Yayama T, Mori K, et al. Increased Cellular Expression of Interleukin-6 in Patients With Ossification of the Posterior Longitudinal Ligament. Spine. 2023;48(6):E78-e86.","journal-title":"Spine"},{"issue":"18","key":"10193_CR34","doi-asserted-by":"publisher","first-page":"5386","DOI":"10.3390\/jcm11185386","volume":"11","author":"K Sakai","year":"2022","unstructured":"Sakai K, Yoshii T, Furuya T, Machino M. Research History, Pathology and Epidemiology of Ossification of the Posterior Longitudinal Ligament and Ligamentum Flavum. J Clin Med. 2022;11(18):5386.","journal-title":"J Clin Med"},{"issue":"3","key":"10193_CR35","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1136\/annrheumdis-2019-215926","volume":"79","author":"H Sun","year":"2020","unstructured":"Sun H, Wen X, Li H, et al. Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration. Ann Rheum Dis. 2020;79(3):408\u201317.","journal-title":"Ann Rheum Dis"},{"issue":"1","key":"10193_CR36","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-025-00463-8","volume":"13","author":"Z Sun","year":"2025","unstructured":"Sun Z, Liu H, Xu Y, et al. Pak4-mediated crosstalk between necroptotic macrophages and tendon stem\/progenitor cells contributes to traumatic heterotopic ossification formation. Bone Res. 2025a;13(1):88.","journal-title":"Bone Res"},{"issue":"1","key":"10193_CR37","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1186\/s12967-025-07562-4","volume":"24","author":"K Sun","year":"2025","unstructured":"Sun K, Yan C, Ji C, et al. Integrin-mediated SPP1 signaling from macrophages orchestrates extracellular matrix remodeling and chondrogenesis in heterotopic ossification. J Transl Med. 2025b;24(1):199.","journal-title":"J Transl Med"},{"issue":"33","key":"10193_CR38","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abn2138","volume":"8","author":"N Tachibana","year":"2022","unstructured":"Tachibana N, Chijimatsu R, Okada H, et al. RSPO2 defines a distinct undifferentiated progenitor in the tendon\/ligament and suppresses ectopic ossification. Sci Adv. 2022;8(33):eabn2138.","journal-title":"Sci Adv"},{"issue":"1","key":"10193_CR39","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.spinee.2024.09.023","volume":"25","author":"M Takahata","year":"2025","unstructured":"Takahata M, Koike Y, Endo T, et al. Adipokine dysregulation as an underlying pathology for diffuse ectopic ossification of spinal posterior longitudinal ligament in patients with obesity. Spine J: Off J North Am Spine Soc. 2025;25(1):80\u201390.","journal-title":"Spine J: Off J North Am Spine Soc"},{"issue":"1","key":"10193_CR40","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1186\/s12951-022-01655-8","volume":"20","author":"Y Tang","year":"2022","unstructured":"Tang Y, Sun Y, Zeng J, et al. Exosomal miR-140-5p inhibits osteogenesis by targeting IGF1R and regulating the mTOR pathway in ossification of the posterior longitudinal ligament. J Nanobiotechnology. 2022;20(1):452.","journal-title":"J Nanobiotechnology"},{"issue":"2","key":"10193_CR41","doi-asserted-by":"publisher","DOI":"10.1002\/jsp2.1247","volume":"6","author":"T Tang","year":"2023","unstructured":"Tang T, Zhu Z, He Z, et al. DLX5 regulates the osteogenic differentiation of spinal ligaments cells derived from ossification of the posterior longitudinal ligament patients via NOTCH signaling. JOR Spine. 2023;6(2):e1247.","journal-title":"JOR Spine"},{"issue":"6","key":"10193_CR42","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1038\/s41592-019-0425-8","volume":"16","author":"L Tian","year":"2019","unstructured":"Tian L, Dong X, Freytag S, et al. Benchmarking single cell RNA-sequencing analysis pipelines using mixture control experiments. Nat Methods. 2019;16(6):479\u201387.","journal-title":"Nat Methods"},{"key":"10193_CR43","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/215140","volume":"2014","author":"T V\u00e4\u00e4n\u00e4nen","year":"2014","unstructured":"V\u00e4\u00e4n\u00e4nen T, Koskinen A, Paukkeri EL, et al. YKL-40 as a novel factor associated with inflammation and catabolic mechanisms in osteoarthritic joints. Mediators Inflamm. 2014;2014:215140.","journal-title":"Mediators Inflamm"},{"issue":"1","key":"10193_CR44","first-page":"191","volume":"21","author":"P Wang","year":"2020","unstructured":"Wang P, Teng Z, Liu X, Liu X, Kong C, Lu S. The COL6A1 rs201153092 single nucleotide polymorphism, associates with thoracic ossification of the posterior longitudinal ligament. Mol Med Rep. 2020;21(1):191\u2013200.","journal-title":"Mol Med Rep"},{"issue":"5","key":"10193_CR45","doi-asserted-by":"publisher","first-page":"1425","DOI":"10.1016\/j.ymthe.2024.03.025","volume":"32","author":"T Wang","year":"2024","unstructured":"Wang T, Kaneko S, Kriukov E, et al. SOCS3 regulates pathological retinal angiogenesis through modulating SPP1 expression in microglia and macrophages. Mol Ther: J Am Soc Gene Ther. 2024;32(5):1425\u201344.","journal-title":"Mol Ther: J Am Soc Gene Ther"},{"issue":"5","key":"10193_CR46","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/j.tem.2022.02.002","volume":"33","author":"X Weng","year":"2022","unstructured":"Weng X, Maxwell-Warburton S, Hasib A, Ma L, Kang L. The membrane receptor CD44: novel insights into metabolism. Trends Endocrinol Metab. 2022;33(5):318\u201332.","journal-title":"Trends Endocrinol Metab"},{"issue":"6","key":"10193_CR47","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.3760\/cma.j.issn.0366-6999.20123323","volume":"126","author":"L Yan","year":"2013","unstructured":"Yan L, Chang Z, Liu Y, Li YB, He BR, Hao DJ. A single nucleotide polymorphism in the human bone morphogenetic protein-2 gene (109T > G) affects the Smad signaling pathway and the predisposition to ossification of the posterior longitudinal ligament of the spine. Chin Med J. 2013;126(6):1112\u20138.","journal-title":"Chin Med J"},{"issue":"1","key":"10193_CR48","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-023-00272-x","volume":"11","author":"JH Yea","year":"2023","unstructured":"Yea JH, Gomez-Salazar M, Onggo S, et al. Tppp3(+) synovial\/tendon sheath progenitor cells contribute to heterotopic bone after trauma. Bone Res. 2023;11(1):39.","journal-title":"Bone Res"}],"container-title":["Cell Biology and Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10565-026-10193-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10565-026-10193-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10565-026-10193-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T03:56:06Z","timestamp":1780026966000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10565-026-10193-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,18]]},"references-count":48,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["10193"],"URL":"https:\/\/doi.org\/10.1007\/s10565-026-10193-0","relation":{},"ISSN":["1573-6822"],"issn-type":[{"value":"1573-6822","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,18]]},"assertion":[{"value":"25 December 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 April 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 April 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Ethical approval for the collection and use of human tissue specimens (No. 2020SYSUSH-055) was granted by the Ethics Committee of the Seventh Affiliated Hospital, Sun Yat-sen University. All animal procedures complied with the International Guide for the Care and Use of Laboratory Animals and were authorized by the Research Ethics Committee of the Sun Yat-Sen University (No. 2020000147).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Written informed consent was obtained from the patient.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Patient consent for publication"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Clinical trial number"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"73"}}