{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T08:03:43Z","timestamp":1777104223287,"version":"3.51.4"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T00:00:00Z","timestamp":1602028800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T00:00:00Z","timestamp":1602028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/100016799","name":"Natural Science Foundation of Guangzhou","doi-asserted-by":"crossref","award":["2016A030313590"],"award-info":[{"award-number":["2016A030313590"]}],"id":[{"id":"10.13039\/100016799","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81500870"],"award-info":[{"award-number":["81500870"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971902"],"award-info":[{"award-number":["81971902"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Foundation Project of Director of Nanfang Hospital of Southern Medical University","award":["2017B013"],"award-info":[{"award-number":["2017B013"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Mol Neurobiol"],"published-print":{"date-parts":[[2022,4]]},"DOI":"10.1007\/s10571-020-00978-0","type":"journal-article","created":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T05:03:45Z","timestamp":1602047025000},"page":"807-816","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Establishment of a Convenient System for the Culture and Study of Perineurium Barrier In Vitro"],"prefix":"10.1007","volume":"42","author":[{"given":"Xuemin","family":"Yin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiao","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodan","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4004-9071","authenticated-orcid":false,"given":"Jin","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,10,7]]},"reference":[{"issue":"3","key":"978_CR1","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.neucir.2012.07.004","volume":"24","author":"R Al-Ghanem","year":"2013","unstructured":"Al-Ghanem R, Ramos-Pleguezuelos FM, P\u00e9rez-Darosa SI, Galicia-Bulnes JM, Cabrerizo-Carvajal F, El-Rubaidi OA (2013) Olfactory ensheathing cell tumour: case report and literature review. Neurocirugia (Astur) 24(3):130\u2013134. https:\/\/doi.org\/10.1016\/j.neucir.2012.07.004","journal-title":"Neurocirugia (Astur)"},{"issue":"6","key":"978_CR2","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1038\/nrc.2016.38","volume":"16","author":"M Amit","year":"2016","unstructured":"Amit M, Na'ara S, Gil Z (2016) Mechanisms of cancer dissemination along nerves. Nat Rev Cancer 16(6):399\u2013408. https:\/\/doi.org\/10.1038\/nrc.2016.38","journal-title":"Nat Rev Cancer"},{"issue":"9","key":"978_CR3","doi-asserted-by":"publisher","first-page":"678","DOI":"10.1097\/00000478-198809000-00004","volume":"12","author":"A Ariza","year":"1988","unstructured":"Ariza A, Bilbao JM, Rosai J (1988) Immunohistochemical detection of epithelial membrane antigen in normal perineurial cells and perineurioma. Am J Surg Pathol 12(9):678\u2013683. https:\/\/doi.org\/10.1097\/00000478-198809000-00004","journal-title":"Am J Surg Pathol"},{"issue":"4","key":"978_CR4","doi-asserted-by":"publisher","first-page":"755","DOI":"10.3892\/mmr.2012.1003","volume":"6","author":"W Chen","year":"2012","unstructured":"Chen W, Dong S, Zhou J, Sun M (2012) Investigation of myoepithelial cell differentiation into Schwann-like cells in salivary adenoid cystic carcinoma associated with perineural invasion. Mol Med Rep 6(4):755\u2013759. https:\/\/doi.org\/10.3892\/mmr.2012.1003","journal-title":"Mol Med Rep"},{"issue":"1","key":"978_CR5","first-page":"1","volume":"9","author":"SH Chen","year":"2019","unstructured":"Chen SH, Zhang BY, Zhou B, Zhu CZ, Sun LQ, Feng YJ (2019) Perineural invasion of cancer: a complex crosstalk between cells and molecules in the perineural niche. Am J Cancer Res 9(1):1\u201321","journal-title":"Am J Cancer Res"},{"issue":"24","key":"978_CR6","doi-asserted-by":"publisher","first-page":"4405","DOI":"10.1007\/s00018-017-2578-x","volume":"74","author":"S Deborde","year":"2017","unstructured":"Deborde S, Wong RJ (2017) How Schwann cells facilitate cancer progression in nerves. Cell Mol Life Sci 74(24):4405\u20134420. https:\/\/doi.org\/10.1007\/s00018-017-2578-x","journal-title":"Cell Mol Life Sci"},{"issue":"4","key":"978_CR7","doi-asserted-by":"publisher","first-page":"1538","DOI":"10.1172\/jci82658","volume":"126","author":"S Deborde","year":"2016","unstructured":"Deborde S, Omelchenko T, Lyubchik A, Zhou Y, He S, McNamara WF, Chernichenko N, Lee SY, Barajas F, Chen CH, Bakst R, Wong RJ (2016) Schwann cells induce cancer cell dispersion and invasion. J Clin Invest 126(4):1538\u20131554. https:\/\/doi.org\/10.1172\/jci82658","journal-title":"J Clin Invest"},{"issue":"2","key":"978_CR8","doi-asserted-by":"publisher","first-page":"e88907","DOI":"10.1371\/journal.pone.0088907","volume":"9","author":"J Deng","year":"2014","unstructured":"Deng J, You Q, Gao Y, Yu Q, Zhao P, Zheng Y, Fang W, Xu N, Teng L (2014) Prognostic value of perineural invasion in gastric cancer: a systematic review and meta-analysis. PLoS ONE 9(2):e88907. https:\/\/doi.org\/10.1371\/journal.pone.0088907","journal-title":"PLoS ONE"},{"issue":"4\u20135","key":"978_CR9","doi-asserted-by":"publisher","first-page":"335","DOI":"10.3109\/01913129109016243","volume":"15","author":"RA Erlandson","year":"1991","unstructured":"Erlandson RA (1991) The enigmatic perineurial cell and its participation in tumors and in tumorlike entities. Ultrastruct Pathol 15(4\u20135):335\u2013351. https:\/\/doi.org\/10.3109\/01913129109016243","journal-title":"Ultrastruct Pathol"},{"key":"978_CR10","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11070893","author":"G Gasparini","year":"2019","unstructured":"Gasparini G, Pellegatta M, Crippa S, Lena MS, Belfiori G, Doglioni C, Taveggia C, Falconi M (2019) Nerves and pancreatic cancer: new insights into a dangerous relationship. Cancers (Basel). https:\/\/doi.org\/10.3390\/cancers11070893","journal-title":"Cancers (Basel)"},{"issue":"4","key":"978_CR11","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1097\/01.Mp.0000062654.83617.B7","volume":"16","author":"T Hirose","year":"2003","unstructured":"Hirose T, Tani T, Shimada T, Ishizawa K, Shimada S, Sano T (2003) Immunohistochemical demonstration of EMA\/Glut1-positive perineurial cells and CD34-positive fibroblastic cells in peripheral nerve sheath tumors. Mod Pathol 16(4):293\u2013298. https:\/\/doi.org\/10.1097\/01.Mp.0000062654.83617.B7","journal-title":"Mod Pathol"},{"issue":"8","key":"978_CR12","doi-asserted-by":"publisher","first-page":"800","DOI":"10.1097\/DAD.0b013e31824ba93b","volume":"34","author":"F Izquierdo","year":"2012","unstructured":"Izquierdo F, Su\u00e1rez-Vilela D, Honrado E (2012) Perineurial cells in granular cell tumors and neoplasms with perineural invasion: an immunohistochemical study. Am J Dermatopathol 34(8):800\u2013809. https:\/\/doi.org\/10.1097\/DAD.0b013e31824ba93b","journal-title":"Am J Dermatopathol"},{"issue":"1","key":"978_CR13","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.adro.2018.09.006","volume":"4","author":"RD Kraus","year":"2019","unstructured":"Kraus RD, Barsky A, Ji L, Garcia Santos PM, Cheng N, Groshen S, Vapiwala N, Ballas LK (2019) The perineural invasion paradox: is perineural invasion an independent prognostic indicator of biochemical recurrence risk in patients with pT2N0R0 Prostate Cancer? A multi-institutional study. Adv Radiat Oncol 4(1):96\u2013102. https:\/\/doi.org\/10.1016\/j.adro.2018.09.006","journal-title":"Adv Radiat Oncol"},{"issue":"2","key":"978_CR14","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.bbcan.2016.01.002","volume":"1865","author":"D Liang","year":"2016","unstructured":"Liang D, Shi S, Xu J, Zhang B, Qin Y, Ji S, Xu W, Liu J, Liu L, Liu C, Long J, Yu X (2016) New insights into perineural invasion of pancreatic cancer: more than pain. Biochim Biophys Acta 1865(2):111\u2013122. https:\/\/doi.org\/10.1016\/j.bbcan.2016.01.002","journal-title":"Biochim Biophys Acta"},{"issue":"15","key":"978_CR15","doi-asserted-by":"publisher","first-page":"3379","DOI":"10.1002\/cncr.24396","volume":"115","author":"C Liebig","year":"2009","unstructured":"Liebig C, Ayala G, Wilks JA, Berger DH, Albo D (2009) Perineural invasion in cancer: a review of the literature. Cancer 115(15):3379\u20133391. https:\/\/doi.org\/10.1002\/cncr.24396","journal-title":"Cancer"},{"key":"978_CR16","doi-asserted-by":"publisher","first-page":"597","DOI":"10.3389\/fnins.2018.00597","volume":"12","author":"Q Liu","year":"2018","unstructured":"Liu Q, Wang X, Yi S (2018) Pathophysiological changes of physical barriers of peripheral nerves after injury. Front Neurosci 12:597. https:\/\/doi.org\/10.3389\/fnins.2018.00597","journal-title":"Front Neurosci"},{"issue":"3","key":"978_CR17","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1016\/j.pathol.2017.09.013","volume":"50","author":"S Lubig","year":"2018","unstructured":"Lubig S, Thiesler T, M\u00fcller S, Vorreuther R, Leipner N, Kristiansen G (2018) Quantitative perineural invasion is a prognostic marker in prostate cancer. Pathology 50(3):298\u2013304. https:\/\/doi.org\/10.1016\/j.pathol.2017.09.013","journal-title":"Pathology"},{"issue":"1","key":"978_CR18","doi-asserted-by":"publisher","first-page":"4284","DOI":"10.1038\/s41467-018-06640-0","volume":"9","author":"M Madeo","year":"2018","unstructured":"Madeo M, Colbert PL, Vermeer DW, Lucido CT, Cain JT, Vichaya EG, Grossberg AJ, Muirhead D, Rickel AP, Hong Z, Zhao J, Vermeer PD (2018) Cancer exosomes induce tumor innervation. Nat Commun 9(1):4284. https:\/\/doi.org\/10.1038\/s41467-018-06640-0","journal-title":"Nat Commun"},{"issue":"3","key":"978_CR19","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1007\/s00401-010-0783-x","volume":"121","author":"AP Mizisin","year":"2011","unstructured":"Mizisin AP, Weerasuriya A (2011) Homeostatic regulation of the endoneurial microenvironment during development, aging and in response to trauma, disease and toxic insult. Acta Neuropathol 121(3):291\u2013312. https:\/\/doi.org\/10.1007\/s00401-010-0783-x","journal-title":"Acta Neuropathol"},{"issue":"18","key":"978_CR20","doi-asserted-by":"publisher","first-page":"4790","DOI":"10.1523\/jneurosci.2875-16.2017","volume":"37","author":"AD Morris","year":"2017","unstructured":"Morris AD, Lewis GM, Kucenas S (2017) Perineurial glial plasticity and the role of TGF-\u03b2 in the development of the blood-nerve barrier. J Neurosci 37(18):4790\u20134807. https:\/\/doi.org\/10.1523\/jneurosci.2875-16.2017","journal-title":"J Neurosci"},{"issue":"8","key":"978_CR21","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1111\/codi.14655","volume":"21","author":"H Nozawa","year":"2019","unstructured":"Nozawa H, Morikawa T, Kawai K, Hata K, Tanaka T, Nishikawa T, Sasaki K, Shuno Y, Kaneko M, Hiyoshi M, Emoto S, Ishihara S (2019) Obstruction is associated with perineural invasion in T3\/T4 colon cancer. Colorectal Dis 21(8):917\u2013924. https:\/\/doi.org\/10.1111\/codi.14655","journal-title":"Colorectal Dis"},{"issue":"2","key":"978_CR22","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1007\/s00261-017-1343-9","volume":"43","author":"BN Patel","year":"2018","unstructured":"Patel BN, Olcott E, Jeffrey RB (2018) Extrapancreatic perineural invasion in pancreatic adenocarcinoma. Abdom Radiol (NY) 43(2):323\u2013331. https:\/\/doi.org\/10.1007\/s00261-017-1343-9","journal-title":"Abdom Radiol (NY)"},{"issue":"5","key":"978_CR23","first-page":"480","volume":"57","author":"J Peltonen","year":"1987","unstructured":"Peltonen J, Jaakkola S, Virtanen I, Pelliniemi L (1987) Perineurial cells in culture. An immunocytochemical and electron microscopic study. Lab Invest 57(5):480\u2013488","journal-title":"Lab Invest"},{"issue":"1","key":"978_CR24","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1369\/jhc.5A6671.2005","volume":"54","author":"KP Pummi","year":"2006","unstructured":"Pummi KP, Aho HJ, Laato MK, Peltonen JT, Peltonen SA (2006) Tight junction proteins and perineurial cells in neurofibromas. J Histochem Cytochem 54(1):53\u201361. https:\/\/doi.org\/10.1369\/jhc.5A6671.2005","journal-title":"J Histochem Cytochem"},{"issue":"10","key":"978_CR25","first-page":"464","volume":"47","author":"MA Reina","year":"2000","unstructured":"Reina MA, L\u00f3pez A, Villanueva MC, de Andr\u00e9s JA, Le\u00f3n GI (2000) Morphology of peripheral nerves, their sheaths, and their vascularization. Rev Esp Anestesiol Reanim 47(10):464\u2013475","journal-title":"Rev Esp Anestesiol Reanim"},{"issue":"2","key":"978_CR26","first-page":"80","volume":"50","author":"MA Reina","year":"2003","unstructured":"Reina MA, L\u00f3pez A, Villanueva MC, De Andr\u00e9s JA, Mach\u00e9s F (2003) The blood-nerve barrier in peripheral nerves. Rev Esp Anestesiol Reanim 50(2):80\u201386","journal-title":"Rev Esp Anestesiol Reanim"},{"issue":"4","key":"978_CR27","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1016\/s0896-6273(02)23442-x","volume":"23","author":"JL Salzer","year":"1999","unstructured":"Salzer JL (1999) Creating barriers: a new role for Schwann cells and Desert hedgehog. Neuron 23(4):627\u2013629. https:\/\/doi.org\/10.1016\/s0896-6273(02)23442-x","journal-title":"Neuron"},{"issue":"6","key":"978_CR28","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1002\/wdev.198","volume":"4","author":"S Sasse","year":"2015","unstructured":"Sasse S, Neuert H, Kl\u00e4mbt C (2015) Differentiation of Drosophila glial cells. Wiley Interdiscip Rev Dev Biol 4(6):623\u2013636. https:\/\/doi.org\/10.1002\/wdev.198","journal-title":"Wiley Interdiscip Rev Dev Biol"},{"key":"978_CR29","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1007\/978-1-60761-938-3_6","volume":"686","author":"A Weerasuriya","year":"2011","unstructured":"Weerasuriya A, Mizisin AP (2011) The blood-nerve barrier: structure and functional significance. Methods Mol Biol 686:149\u2013173. https:\/\/doi.org\/10.1007\/978-1-60761-938-3_6","journal-title":"Methods Mol Biol"},{"issue":"2","key":"978_CR30","doi-asserted-by":"publisher","first-page":"204","DOI":"10.7518\/hxkq.2018.02.017","volume":"36","author":"M Zhang","year":"2018","unstructured":"Zhang M, Liang XH, Tang YL (2018) Advances in the molecular mechanisms of perineural invasion in salivary adenoid cystic carcinoma. Hua Xi Kou Qiang Yi Xue Za Zhi 36(2):204\u2013211. https:\/\/doi.org\/10.7518\/hxkq.2018.02.017","journal-title":"Hua Xi Kou Qiang Yi Xue Za Zhi"}],"container-title":["Cellular and Molecular Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10571-020-00978-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10571-020-00978-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10571-020-00978-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T11:19:54Z","timestamp":1646219994000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10571-020-00978-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,7]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["978"],"URL":"https:\/\/doi.org\/10.1007\/s10571-020-00978-0","relation":{},"ISSN":["0272-4340","1573-6830"],"issn-type":[{"value":"0272-4340","type":"print"},{"value":"1573-6830","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,7]]},"assertion":[{"value":"22 June 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 September 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 October 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethical Standards"}},{"value":"The authors declare that they have no conflicts of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"No human participants or animals were involved in this paper.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Research Involving Human and\/or Animal Rights"}}]}}