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In addition, an alternative anatomical route at the choroid plexus attachment region has been proposed, yet its structural organization remains poorly defined. Here, we provide a detailed descriptive analysis of the choroid plexus attachment region and identify glial structures reminiscent of a glia limitans. We analyzed the morphology of the choroid plexus attachment region in control mice using immunohistochemical and ultrastructural techniques. Immune cell accumulation under neuroinflammatory conditions was examined in the CupEAE mouse model, and human post-mortem sections were analyzed for comparative insights. Our results show that the murine choroid plexus attachment region contains an astrocytic accumulation in close proximity to a continuous basal lamina facing the choroid plexus stroma. Ultrastructural analyses indicate the presence of components associated with junctional complexes. In the CupEAE model, CD3-positive immune cells were more frequently observed in the choroid plexus base than in the brain parenchyma adjacent to the attachment region. In humans, choroid plexus attachment regions also exhibit astrocytic accumulation, though with a different basal lamina pattern. These results underline the complex organization of the choroid plexus attachment region. This anatomical description might be the basis for further studies to evaluate the relevance of the choroid plexus attachment region in immune cell migration to the brain parenchyma.<\/jats:p>","DOI":"10.1007\/s10571-026-01742-6","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T21:14:37Z","timestamp":1779138877000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Astrocytic Accumulation at the Choroid Plexus Attachment Region"],"prefix":"10.1007","volume":"46","author":[{"given":"Katerina","family":"Manzhula","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5549-7534","authenticated-orcid":false,"given":"Theresa","family":"Greiner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise","family":"Baumann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannes","family":"Kaddatz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9559-7492","authenticated-orcid":false,"given":"Vladislav","family":"Yakimov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2517-4440","authenticated-orcid":false,"given":"Marcus","family":"Frank","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bj\u00f6rn","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5043-9052","authenticated-orcid":false,"given":"Markus","family":"Kipp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8654-8904","authenticated-orcid":false,"given":"Sarah","family":"Joost","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,5,18]]},"reference":[{"key":"1742_CR1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17204-5","volume":"7","author":"P Bankhead","year":"2017","unstructured":"Bankhead P, Loughrey MB, Fern\u00e1ndez JA, Dombrowski Y, McArt DG, Dunne PD, McQuaid S, Gray RT, Murray LJ, Coleman HG, James JA, Salto-Tellez M, Hamilton PW (2017) QuPath: open source software for digital pathology image analysis. 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The animal experiments were authorized in accordance with \u00a7\u00a08 of the German Animal Welfare Act (reference number of the Landesamt f\u00fcr Landwirtschaft, Lebensmittelsicherheit und Fischerei Mecklenburg-Vorpommern 7221.3-1-001\/19 and reference number of the Regierung Oberbayern 55.2-154-2532-73-15) and all applicable animal welfare guidelines were complied with.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval and Consent to Participate"}}],"article-number":"88"}}