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Occludin protein expression by immunohistochemistry was analyzed in 602 HGSC (417 effusions, 185 surgical specimens). Expression in mesothelioma (n\u2009=\u200987; 45 effusions, 42 surgical specimens) was studied for comparative purposes. Occludin protein expression was found in 587\/602 (98%) HGSC vs. 40\/87 (46%) mesotheliomas and was predominantly limited to\u2009&lt;\u20095% of cells in the latter (p\u2009&lt;\u20090.001). Occludin was additionally overexpressed in HGSC effusions compared to surgical specimens (p\u2009&lt;\u20090.001) and was overexpressed in post-chemotherapy\u00a0effusions\u00a0compared to\u00a0chemo-naive effusions tapped at diagnosis (p\u2009=\u20090.015). Occludin expression in HGSC surgical specimens was associated with poor chemoresponse (p\u2009&lt;\u20090.001) and primary resistance (p\u2009=\u20090.001). Expression in effusions and surgical specimens was unrelated to survival (p\u2009&gt;\u20090.05). In conclusion, occludin expression is higher in HGSC compared to mesothelioma, and this protein is overexpressed in HGSC effusions, possibly reflecting changes in adhesion related to anchorage-independent growth in this microenvironment. Overexpression in post-chemotherapy compared to chemo-na\u00efve effusions suggest a role in disease progression. Occludin expression in surgical specimens may be related to chemoresistance.<\/jats:p>","DOI":"10.1007\/s10585-023-10251-5","type":"journal-article","created":{"date-parts":[[2023,12,23]],"date-time":"2023-12-23T07:02:19Z","timestamp":1703314939000},"page":"69-76","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Occludin is overexpressed in tubo-ovarian high-grade serous carcinoma compared to mesothelioma and is a marker of tumor progression and chemoresistance"],"prefix":"10.1007","volume":"41","author":[{"given":"Margarida Varela","family":"dos Santos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arild","family":"Holth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharina","family":"Bischof","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3332-8427","authenticated-orcid":false,"given":"Ben","family":"Davidson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,12,23]]},"reference":[{"issue":"3","key":"10251_CR1","doi-asserted-by":"publisher","first-page":"209","DOI":"10.3322\/caac.21660","volume":"71","author":"H Sung","year":"2021","unstructured":"Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F (2021) Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. 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