{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T21:13:30Z","timestamp":1792876410365,"version":"4.5.0"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100013845","name":"Fondation contre le Cancer","doi-asserted-by":"publisher","award":["C\/2020\/1441"],"award-info":[{"award-number":["C\/2020\/1441"]}],"id":[{"id":"10.13039\/501100013845","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Clin Exp Metastasis"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Metastatic breast cancer (mBC) remains incurable and liver metastases (LM) are observed in approximately 50% of all patients with mBC. In some cases, surgical resection of breast cancer liver metastases (BCLM) is associated with prolonged survival. However, there are currently no validated marker to identify these patients. The interactions between the metastatic cancer cells and the liver microenvironment result in two main histopathological growth patterns (HGP): replacement (r-HGP), characterized by a direct contact between the cancer cells and the hepatocytes, and desmoplastic (d-HGP), in which a fibrous rim surrounds the tumor cells. In patients who underwent resection of BCLM, the r-HGP is associated with a worse postoperative prognosis than the d-HGP. Here, we aim at unraveling the biological differences between these HGP within ten patients presenting both HGP within the same metastasis. The transcriptomic analyses reveal overexpression of genes involved in cell cycle, DNA repair, vessel co-option and cell motility in r-HGP while angiogenesis, wound healing, and several immune processes were found overexpressed in d-HGP LM. Understanding the biology of the LM could open avenues to refine treatment of BC patients with LM.<\/jats:p>","DOI":"10.1007\/s10585-024-10279-1","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T23:01:45Z","timestamp":1711666905000},"page":"699-705","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Transcriptomic characterization of the histopathological growth patterns in breast cancer liver metastases"],"prefix":"10.1007","volume":"41","author":[{"given":"Sophia","family":"Leduc","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ha-Linh","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Richard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gitte","family":"Zels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amena","family":"Mahdami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxim","family":"De Schepper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Maetens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anirudh","family":"Pabba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joris","family":"Jaekers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Latacz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Bohlok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evy","family":"Vanderheyden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Van Brussel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bram","family":"Boeckx","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rogier","family":"Schepers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diether","family":"Lambrechts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Dirix","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Larsimont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Vankerckhove","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerio","family":"Lucidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baki","family":"Topal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imane","family":"Bachir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Donckier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Floris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Vermeulen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Desmedt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,29]]},"reference":[{"key":"10279_CR1","doi-asserted-by":"publisher","DOI":"10.1038\/S41572-019-0111-2","author":"N Harbeck","year":"2019","unstructured":"Harbeck N, Penault-Llorca F, Cortes J et al (2019) Breast cancer. Nat Rev Dis Primers. https:\/\/doi.org\/10.1038\/S41572-019-0111-2. 5:","journal-title":"Nat Rev Dis Primers"},{"key":"10279_CR2","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1097\/01.SLA.0000246847.02058.1B","volume":"244","author":"R Adam","year":"2006","unstructured":"Adam R, Aloia T, Krissat J et al (2006) Is liver resection justified for patients with hepatic metastases from breast Cancer? Ann Surg 244:897. https:\/\/doi.org\/10.1097\/01.SLA.0000246847.02058.1B","journal-title":"Ann Surg"},{"key":"10279_CR3","doi-asserted-by":"publisher","first-page":"1341","DOI":"10.3390\/cancers11091341","volume":"11","author":"R Bale","year":"2019","unstructured":"Bale R, Putzer D, Schullian P (2019) Local treatment of breast Cancer Liver Metastasis. Cancers (Basel) 11:1341. https:\/\/doi.org\/10.3390\/cancers11091341","journal-title":"Cancers (Basel)"},{"key":"10279_CR4","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1159\/000012098","volume":"58","author":"X Pivot","year":"2000","unstructured":"Pivot X, Asmar L, Hortobagyi GN et al (2000) A retrospective study of first indicators of breast Cancer recurrence. Oncology 58:185\u2013190. https:\/\/doi.org\/10.1159\/000012098","journal-title":"Oncology"},{"key":"10279_CR5","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/J.JSS.2008.12.030","volume":"161","author":"AM Leung","year":"2010","unstructured":"Leung AM, Vu HN, Nguyen KA et al (2010) Effects of Surgical Excision on Survival of patients with stage IV breast Cancer. J Surg Res 161:83\u201388. https:\/\/doi.org\/10.1016\/J.JSS.2008.12.030","journal-title":"J Surg Res"},{"key":"10279_CR6","doi-asserted-by":"publisher","first-page":"276","DOI":"10.3816\/CBC.2001.N.002","volume":"1","author":"M Cristofanilli","year":"2001","unstructured":"Cristofanilli M, Hortobagyi GN (2001) New Horizons in treating metastatic disease. Clin Breast Cancer 1:276\u2013287. https:\/\/doi.org\/10.3816\/CBC.2001.N.002","journal-title":"Clin Breast Cancer"},{"key":"10279_CR7","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1007\/S10549-016-3974-X\/FIGURES\/2","volume":"160","author":"LG Eng","year":"2016","unstructured":"Eng LG, Dawood S, Sopik V et al (2016) Ten-year survival in women with primary stage IV breast cancer. Breast Cancer Res Treat 160:145\u2013152. https:\/\/doi.org\/10.1007\/S10549-016-3974-X\/FIGURES\/2","journal-title":"Breast Cancer Res Treat"},{"key":"10279_CR8","doi-asserted-by":"publisher","unstructured":"Leduc S, De Schepper M, Vermeulen P et al (2023) Histopathological growth patterns and tumor infiltrating lymphocytes in breast cancer liver metastases. NPJ Breast Cancer Press 83. https:\/\/doi.org\/10.1158\/1538-7445.SABCS22-P6-14-06. P6-14-06","DOI":"10.1158\/1538-7445.SABCS22-P6-14-06"},{"key":"10279_CR9","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1038\/s41523-020-00209-1","volume":"6","author":"A Bohlok","year":"2020","unstructured":"Bohlok A, Vermeulen P, Leduc S et al (2020) Association between the histopathological growth patterns of liver metastases and survival after hepatic surgery in breast cancer patients. NPJ Breast Cancer 6:64. https:\/\/doi.org\/10.1038\/s41523-020-00209-1","journal-title":"NPJ Breast Cancer"},{"issue":"6","key":"10279_CR10","doi-asserted-by":"publisher","first-page":"988","DOI":"10.1038\/s41416-022-01859-7","volume":"127","author":"E Latacz","year":"2022","unstructured":"Latacz E, H\u00f6ppener D, Bohlok A et al (2022) Histopathological growth patterns of liver metastasis: updated consensus guidelines for pattern scoring, perspectives and recent mechanistic insights. Br J Cancer 2022 127(6):988\u20131013. https:\/\/doi.org\/10.1038\/s41416-022-01859-7","journal-title":"Br J Cancer 2022"},{"key":"10279_CR11","doi-asserted-by":"publisher","first-page":"5024","DOI":"10.1038\/s41467-023-40688-x","volume":"14","author":"C Fern\u00e1ndez Moro","year":"2023","unstructured":"Fern\u00e1ndez Moro C, Geyer N, Harrizi S et al (2023) An idiosyncratic zonated stroma encapsulates desmoplastic liver metastases and originates from injured liver. Nat Commun 14:5024. https:\/\/doi.org\/10.1038\/s41467-023-40688-x","journal-title":"Nat Commun"},{"key":"10279_CR12","doi-asserted-by":"publisher","first-page":"1427","DOI":"10.1038\/BJC.2017.334","volume":"117","author":"PJ Van Dam","year":"2017","unstructured":"Van Dam PJ, Van Der Stok EP, Teuwen LA et al (2017) International consensus guidelines for scoring the histopathological growth patterns of liver metastasis. Br J Cancer 117:1427\u20131441. https:\/\/doi.org\/10.1038\/BJC.2017.334","journal-title":"Br J Cancer"},{"key":"10279_CR13","unstructured":"Qiagen (2018) miRNeasy FFPE Handbook"},{"key":"10279_CR14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/S13059-017-1349-1\/FIGURES\/4","volume":"18","author":"D Aran","year":"2017","unstructured":"Aran D, Hu Z, Butte AJ (2017) xCell: digitally portraying the tissue cellular heterogeneity landscape. Genome Biol 18:1\u201314. https:\/\/doi.org\/10.1186\/S13059-017-1349-1\/FIGURES\/4","journal-title":"Genome Biol"},{"key":"10279_CR15","doi-asserted-by":"publisher","first-page":"113379","DOI":"10.1016\/j.ejca.2023.113379","volume":"195","author":"C Bosi","year":"2023","unstructured":"Bosi C, Bartha \u00c1, Galbardi B et al (2023) Pan-cancer analysis of antibody-drug conjugate targets and putative predictors of treatment response. Eur J Cancer 195:113379. https:\/\/doi.org\/10.1016\/j.ejca.2023.113379","journal-title":"Eur J Cancer"},{"key":"10279_CR16","doi-asserted-by":"publisher","unstructured":"Dong BC, Luo X, Qi C et al (2023) Targeting pioneer transcription factor Ascl1 to promote optic nerve regeneration. https:\/\/doi.org\/10.1101\/2023.07.20.549959. BioRxive","DOI":"10.1101\/2023.07.20.549959"},{"key":"10279_CR17","doi-asserted-by":"publisher","unstructured":"Garc\u00eda-G\u00f3mez P Valiente \u00b7 Manuel (123AD) vascular co-option in brain metastasis. Angiogenesis 23:3\u20138. https:\/\/doi.org\/10.1007\/s10456-019-09693-x","DOI":"10.1007\/s10456-019-09693-x"},{"key":"10279_CR18","doi-asserted-by":"publisher","first-page":"1294","DOI":"10.1038\/nm.4197","volume":"22","author":"S Frentzas","year":"2016","unstructured":"Frentzas S, Simoneau E, Bridgeman VL et al (2016) Vessel co-option mediates resistance to anti-angiogenic therapy in liver metastases. Nat Med 22:1294\u20131302. https:\/\/doi.org\/10.1038\/nm.4197","journal-title":"Nat Med"},{"key":"10279_CR19","doi-asserted-by":"publisher","unstructured":"Lingg L, Rottenberg S, Francica P (2022) Meiotic genes and DNA double strand break repair in Cancer. Front Genet 13. https:\/\/doi.org\/10.3389\/FGENE.2022.831620","DOI":"10.3389\/FGENE.2022.831620"},{"key":"10279_CR20","doi-asserted-by":"publisher","unstructured":"Hu M, Chen Z, Hu D et al (2022) Delineating the molecular landscape of different histopathological growth patterns in colorectal cancer liver metastases. Front Immunol 13:1045329. https:\/\/doi.org\/10.3389\/FIMMU.2022.1045329\/FULL","DOI":"10.3389\/FIMMU.2022.1045329\/FULL"},{"key":"10279_CR21","doi-asserted-by":"publisher","unstructured":"Stremitzer S, Vermeulen P, Graver S et al (2020) Immune phenotype and histopathological growth pattern in patients with colorectal liver metastases. Br J Cancer 122:1518\u20131524. https:\/\/doi.org\/10.1038\/s41416-020-0812-z","DOI":"10.1038\/s41416-020-0812-z"},{"key":"10279_CR22","doi-asserted-by":"publisher","unstructured":"Liang J-Y, Xi S-Y, Shao Q et al (2020) Histopathological growth patterns correlate with the immunoscore in colorectal cancer liver metastasis patients after hepatectomy. 69:2623\u20132634. https:\/\/doi.org\/10.1007\/s00262-020-02632-6","DOI":"10.1007\/s00262-020-02632-6"},{"key":"10279_CR23","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1038\/s41416-020-0881-z","volume":"123","author":"DJ H\u00f6ppener","year":"2020","unstructured":"H\u00f6ppener DJ, Nierop PMH, Hof J et al (2020) Enrichment of the tumour immune microenvironment in patients with desmoplastic colorectal liver metastasis. Br J Cancer 123:196\u2013206. https:\/\/doi.org\/10.1038\/s41416-020-0881-z","journal-title":"Br J Cancer"},{"issue":"13","key":"10279_CR24","doi-asserted-by":"publisher","first-page":"4587","DOI":"10.1245\/s10434-019-07910-x","volume":"26","author":"J Cheng","year":"2019","unstructured":"Cheng J, Wei J, Tong T et al (2019) Prediction of histopathologic growth patterns of Colorectal Liver metastases with a noninvasive imaging method. Ann Surg Oncol 26(13):4587\u20134598. https:\/\/doi.org\/10.1245\/s10434-019-07910-x","journal-title":"Ann Surg Oncol"},{"issue":"5","key":"10279_CR25","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1007\/s10585-021-10119-6","volume":"38","author":"MPA Starmans","year":"2021","unstructured":"Starmans MPA, Buisman FE, Renckens M et al (2021) Distinguishing pure histopathological growth patterns of colorectal liver metastases on CT using deep learning and radiomics: a pilot study. Clin Exp Metastasis 38(5):483\u2013494. https:\/\/doi.org\/10.1007\/s10585-021-10119-6","journal-title":"Clin Exp Metastasis"}],"container-title":["Clinical &amp; Experimental Metastasis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10585-024-10279-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10585-024-10279-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10585-024-10279-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T00:44:08Z","timestamp":1729730648000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10585-024-10279-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,29]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10279"],"URL":"https:\/\/doi.org\/10.1007\/s10585-024-10279-1","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-3708512\/v1","asserted-by":"object"}]},"ISSN":["0262-0898","1573-7276"],"issn-type":[{"value":"0262-0898","type":"print"},{"value":"1573-7276","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,29]]},"assertion":[{"value":"5 December 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 February 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 March 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The Authors declare no Competing Financial or Non-Financial Interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}