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Antineoplastic drugs, such as cyclophosphamide and imatinib, are particularly concerning due to their cytotoxic effects at low concentrations. The purpose of this study was to determine the bioavailability and toxicity of these 2 drugs to freshwater mussels, known to thrive in urban areas. Freshwater mussels\n                    <jats:italic>Elliptio complanata<\/jats:italic>\n                    were exposed to increasing concentrations (4, 20, and 100 \u00b5gL\n                    <jats:sup>\u2212\u20091<\/jats:sup>\n                    ) of cyclophosphamide and imatinib for 96\u00a0h at 15\u00a0\u00b0C. Although a low bioaccumulation factor of 0.27 was measured for cyclophosphamide, its level in tissues was correlated with lipid peroxidation in gonads and gonadosomatic index (GSI), which could suggest that it is involved in oxidative stress and increased GSI. The metabolite carboxyphosphamide was never detected in mussel tissue. Imatinib was more bioavailable with a measured bioaccumulation factor of 2.3. Tissue levels of imatinib were correlated with DNA damage (DNAd) in both the digestive gland and gonads while decreased acetylcholine esterase activity was observed, suggesting that it is involved in decreased neural activity and DNAd in mussels. Based on these findings, imatinib could disrupt the repair of oxidized DNAd, leading to the accumulation of alkali-labile sites in DNA. While the short-term exposures resulted in low cytostatic concentrations in mussel tissues, chronic exposure from continuous wastewater releases with the rising use of cytostatic drugs for cancer treatments could still pose a potential environmental risk that warrants further investigation.\n                  <\/jats:p>","DOI":"10.1007\/s10646-025-02976-8","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T09:07:16Z","timestamp":1760087236000},"page":"2112-2121","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioaccumulation and toxicity of imatinib, cyclophosphamide and its transformation product carboxyphosphamide in the freshwater mussel Elliptio complanata"],"prefix":"10.1007","volume":"34","author":[{"given":"E","family":"Eysseric","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Vlassopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Andr\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Gagn\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Gagnon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,10]]},"reference":[{"key":"2976_CR1","doi-asserted-by":"publisher","unstructured":"Boxall ABA, Rudd MA, Brooks BW, Caldwell DJ, Choi K, Hickmann S, Innes E, Ostapyk K, Staveley JP, Verslycke T, Ankley GT, Beazley KF, Belanger SE, Berninger JP, Carriquiriborde P, Coors A, DeLeo PC, Dyer SD, Ericson JF, Gagn\u00e9 F, Giesy JP, Gouin T, Hallstrom L, Karlsson MV, Larsson DGJ, Lazorchak JM, Mastrocco F, McLaughlin A, McMaster ME, Meyerhoff RD, Moore R, Parrott JL, Snape JR, Murray-Smith R, Servos MR, Sibley PK, Straub JO, Szabo ND, Topp E, Tetreault GR, Trudeau VL (2012) Van der Kraak, G. 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