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While glyphosate targets the shikimate pathway in plants, which is absent in animals, there is increasing evidence that glyphosate induces sublethal effects on arthropod predators. However, the factors modulating these effects remain unclear. Here, we investigated how physiological context, specifically pre-treatment with the broad-spectrum antibiotic tetracycline, commonly used to experimentally alter host associated microbial communities, modulates the sublethal hazard of glyphosate-based herbicide (GBH) on the predatory behavior of adult female\n                    <jats:italic>Pardosa<\/jats:italic>\n                    wolf spiders. Predation efficiency was measured using functional response assays across varying prey densities. Our results showed that GBH significantly reduced the spiders\u2019 predation rates, confirming its sublethal toxicity. However, spiders pre-treated with antibiotics exhibited higher capture rates following GBH exposure compared to the GBH only group. These findings demonstrate that the sublethal hazard of GBH on predators is context-dependent and can be altered by antibiotic-sensitive processes (such as host-symbiont interactions). This study highlights the complexity of pesticide effects on ecosystem services and suggests that the functional response of non-target predators depends strongly on their physiological background.\n                  <\/jats:p>","DOI":"10.1007\/s10646-026-03179-5","type":"journal-article","created":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:35:14Z","timestamp":1792740914000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Context-dependent sublethal hazard of glyphosate to non-target predators revealed by functional response analysis"],"prefix":"10.1007","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6767-4064","authenticated-orcid":false,"given":"Jana","family":"Niedobov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radek","family":"Michalko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,23]]},"reference":[{"key":"3179_CR2","doi-asserted-by":"publisher","first-page":"827384","DOI":"10.3389\/fnut.2022.827384","volume":"9","author":"JA Barnett","year":"2022","unstructured":"Barnett JA, Bandy ML, Gibson DL (2022) Is the use of glyphosate in modern agriculture resulting in increased neuropsychiatric conditions through modulation of the gut-brain-microbiome axis? 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